Format:
1 Online-Ressource (XII, 538 Seiten)
,
Illustrationen
Edition:
Second edition
ISBN:
9781118382912
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1283644274
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9781283644273
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9780471692140
Content:
Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags.The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy. PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website: www.wiley.com/go/murphy/lightmicroscopy.
Content:
Intro -- Title page -- Copyright page -- Contents -- Preface -- Acknowledgments -- CHAPTER 1: Fundamentals of Light Microscopy -- Overview -- Optical Components of the Light Microscope -- Aperture and Image Planes in a Focused, Adjusted Microscope -- Koehler Illumination -- Adjusting the Microscope for Koehler Illumination -- Fixed Tube Length versus Infinity Optical Systems -- Precautions for Handling Optical Equipment -- Care and Maintenance of the Microscope -- CHAPTER 2: Light and Color -- Overview -- Light as a Probe of Matter -- The Dual Particle- and Wave-Like Nature of Light -- The Quality of Light -- Properties of Light Perceived by the Eye -- Physical Basis for Visual Perception and Color -- Addition and Subtraction Colors -- CHAPTER 3: Illuminators, Filters, and the Isolation of Specific Wavelengths -- Overview -- Illuminators and Their Spectra -- Illuminator Alignment and Bulb Replacement -- Filters for Adjusting the Intensity and Wavelength of Illumination -- Neutral Density Filters -- Colored Glass Filters -- Interference Filters -- Effects of Light on Living Cells -- CHAPTER 4: Lenses and Geometrical Optics -- Overview -- Reflection and Refraction of Light -- Image Formation by a Simple Lens -- Rules of Ray Tracing for a Simple Lens -- Object-Image Math -- The Principal Aberrations of Lenses -- Designs and Specifications of Objectives -- Special Objective Designs -- Markings on the Barrel of an Objective -- Image Brightness -- Objective Parfocality -- Condensers -- Oculars -- Microscope Slides and Coverslips -- The Care and Cleaning of Optics -- Dust -- Immersion Oil -- Scratches and Abrasions -- Mechanical Force -- CHAPTER 5: Diffraction and Interference in Image Formation -- Overview -- Diffraction and Interference -- The Diffraction Image of a Point Source of Light -- The Constancy of Optical Path Length between Object and Image.
Note:
Description based on publisher supplied metadata and other sources
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Fundamentals of light microscopyLight and color -- Illuminators, filters, and the isolation of specific wavelengths -- Lenses and geometrical optics -- Diffraction and interference in image formation -- Diffraction and spatial resolution -- Phase contrast microscopy and darkfield microscopy -- Properties of polarized light -- Polarization microscopy -- Differential interference contrast (DIC) microscopy and modulation contrast microscopy -- Fluorescence microscopy -- Fluorescence imaging of dynamic molecular processes -- Confocal laser scanning microscopy -- Two-photon excitation fluorescence microscopy -- Superresolution imaging -- Imaging living cells with the microscope -- Fundamentals of digital imaging -- Digital imaging processing.
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Title page; Copyright page; Contents; Preface; Acknowledgments; CHAPTER 1: Fundamentals of Light Microscopy; Overview; Optical Components of the Light Microscope; Aperture and Image Planes in a Focused, Adjusted Microscope; Koehler Illumination; Adjusting the Microscope for Koehler Illumination; Fixed Tube Length versus Infinity Optical Systems; Precautions for Handling Optical Equipment; Care and Maintenance of the Microscope; CHAPTER 2: Light and Color; Overview; Light as a Probe of Matter; The Dual Particle- and Wave-Like Nature of Light; The Quality of Light
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Properties of Light Perceived by the EyePhysical Basis for Visual Perception and Color; Addition and Subtraction Colors; CHAPTER 3: Illuminators, Filters, and the Isolation of Specific Wavelengths; Overview; Illuminators and Their Spectra; Illuminator Alignment and Bulb Replacement; Filters for Adjusting the Intensity and Wavelength of Illumination; Neutral Density Filters; Colored Glass Filters; Interference Filters; Effects of Light on Living Cells; CHAPTER 4: Lenses and Geometrical Optics; Overview; Reflection and Refraction of Light; Image Formation by a Simple Lens
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Rules of Ray Tracing for a Simple LensObject-Image Math; The Principal Aberrations of Lenses; Designs and Specifications of Objectives; Special Objective Designs; Markings on the Barrel of an Objective; Image Brightness; Objective Parfocality; Condensers; Oculars; Microscope Slides and Coverslips; The Care and Cleaning of Optics; Dust; Immersion Oil; Scratches and Abrasions; Mechanical Force; CHAPTER 5: Diffraction and Interference in Image Formation; Overview; Diffraction and Interference; The Diffraction Image of a Point Source of Light
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The Constancy of Optical Path Length between Object and ImageEffect of Aperture Angle on Diffraction Spot Size; Diffraction by a Grating and Calculation of Its Line Spacing, D; Abbé's Theory for Image Formation in the Microscope; A Diffraction Pattern Is Formed in the Rear Aperture of the Objective; Preservation of Coherence: Essential Requirement for Image Formation; CHAPTER 6: Diffraction and Spatial Resolution; Overview; Numerical Aperture; Spatial Resolution; Depth of Field and Depth of Focus; Optimizing the Microscope Image: A Compromise between Spatial Resolution and Contrast
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CHAPTER 7: Phase Contrast Microscopy and Darkfield MicroscopyOverview; Phase Contrast Microscopy; The Behavior of Waves from Phase Objects in Brightfield Microscopy; Wave Terminology and the Importance of Coherence; Depiction of Wave Interactions with Sine Wave and Vector Diagrams; The Role of Differences in Optical Path Lengths; The Optical Design of the Phase Contrast Microscope; Alignment; Interpreting the Phase Contrast Image; Darkfield Microscopy; Theory and Optics; Image Interpretation; CHAPTER 8: Properties of Polarized Light; Overview; The Generation of Polarized Light
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Polarization by Reflection and Scattering
Additional Edition:
ISBN 9780471692140
Additional Edition:
Erscheint auch als Druck-Ausgabe Murphy, Douglas B., 1945 - Fundamentals of light microscopy and electronic imaging Hoboken, NJ : Wiley-Blackwell, 2013 ISBN 9780471692140
Additional Edition:
ISBN 047169214X
Language:
English
Subjects:
Physics
Keywords:
Mikroskopie
URL:
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