Format:
Online-Ressource (X, 476 p. 45 illus., 23 illus. in color, digital)
ISBN:
9783642294679
Series Statement:
Principles and Practice
Content:
This book provides a state-of-the-art approach to the molecular basis of hematologic diseases and its translation into improved diagnostics and novel therapeutic strategies. Several representative hemato-oncologic malignancies are analyzed in detail: acute lymphoblastic leukemia, acute myeloid leukemia, B-cell Non-Hodgkin lymphomas, multiple myeloma, chronic lymphocytic leukemia, chronic myeloid leukemia, myelodysplastic syndromes, and myeloproliferative neoplasms. Experts in the field describe the molecular methods applied for modern diagnostics and therapies, such as hematopoietic stem cell transplantation, donor recipient matching, banking of biological material, analyses of post-transplant chimerism, and minimal residual disease monitoring. The volume concludes with an extensive section comprising thorough step-by-step protocols of molecular techniques in hematology, all of them validated in the authors own laboratories.
Note:
Description based upon print version of record
,
Molecular Aspectsof HematologicMalignancies; Preface; Contents; Part I Molecular Biology of SelectedHemato-oncologic Diseases; 1 Molecular Biology of Acute Lymphoblastic Leukemia; Abstract; 1.1…Introduction; 1.2…Etiopathogenesis; 1.3…Classification of ALL and Risk Factors; 1.3.1 Morphological Versus Immunophenotypic Classification of ALL; 1.3.2 Conventional Versus Molecular-Based Prognostic Factors in ALL; 1.3.2.1 Chromosomal Abnormalities in BCP-ALL; 1.3.2.2 Chromosomal Abnormalities in T-ALL; 1.3.2.3 Minimal Residual Disease; 1.4…Implications of Recent Findings on Molecular Biology of ALL
,
1.4.1 Copy Number Alterations1.4.1.1 IKZF1; 1.4.1.2 PAX5; 1.4.2 Gene Expression Profiling; 1.4.2.1 Gene Expression Profiling for Improved Prognostic Classification of ALL; 1.4.2.2 Gene Expression Profiling of T-ALL; 1.4.3 Epigenetics; 1.4.3.1 CpG Island Methylation in Infant MLL-r ALL; 1.4.3.2 CpG Island Methylation in T-ALL; 1.4.4 Point Mutations in T-ALL; 1.4.4.1 NOTCH1; 1.4.4.2 FBXW7; 1.5…Immunoglobulin and T-Cell Receptor Gene Rearrangements; 1.5.1 Immunoglobulin/T-Cell Receptor Gene Rearrangements in Normal Cells: Implications for ALL Study; 1.5.2 Ig/TCR Gene Rearrangements in BCP-ALL
,
1.5.3 Ig/TCR Gene Rearrangements in T-ALL1.6…Conclusions; Acknowledgments; References; 2 Genetic Mechanisms and Molecular Markers of Neoplastic Transformation in Acute Myeloid Leukemia; Abstract; 2.1…Introduction; 2.2…Genetic Pathomechanisms of Acute Myeloid Leukemia; 2.2.1 Classical ''Two Hits'' Model of AML Transformation; 2.2.2 Extension of ''Two Hits'' Model by Other Mechanisms Leading to Leukemogenesis; 2.3…Gross Genetic Alterations in AML; 2.4…Cryptic Molecular Alterations Associated with Cytogenetically Normal AML; 2.4.1 Intragenic Aberrations; 2.4.1.1 FLT3; 2.4.1.2 NPM1; 2.4.1.3 CEBPA
,
2.4.1.4 MLL2.4.1.5 IDH1/2; 2.4.1.6 TET2; 2.4.1.7 Other Intragenic Aberrations Associated with CN-AML; 2.4.2 Gene Overexpression; 2.4.2.1 BAALC; 2.4.2.2 ERG; 2.4.2.3 MN1; 2.5…Intragenic Aberrations Associated with Cytogenetically Abnormal AML; 2.5.1 C-KIT; 2.5.2 N-RAS and K-RAS; 2.6…MicroRNAs in AML; 2.7…Acquired Copy Number Alterations Identified by SNP Arrays; 2.8…Epigenetic Mechanisms in AML; 2.9…Clinical Implications of Molecular Genetics in AML; 2.9.1 Risk Stratification Based on Cytogenetic and Molecular Features of AML; 2.9.2 Targeted Therapy of AML
,
2.9.3 Monitoring of Minimal Residual Disease2.10…Conclusions; Acknowledgments; References; 3 Molecular Pathogenesis of Aggressive B-cell Lymphomas; Abstract; 3.1…Introduction; 3.2…Cellular Origin of B-cell Lymphomas; 3.3…Transforming Events in B-cell Lymphomas; 3.4…Molecular Heterogeneity of DLBCL; 3.4.1 Similarities of DLBCL Cells to Putative Cell of Origin (COO Classification); 3.4.2 Classification of DLBCLs Without a Priori Assumptions (Comprehensive Clusters Classification, CCC); 3.5…Translating Basic Science to New Molecularly Targeted Therapies; 3.6…Conclusions; References
,
4 Molecular Biology and Classification of Multiple Myeloma
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Part 1.Molecular Biology of Selected Hemato-oncologic DiseasesMolecular Biology of Acute Lymphoblastic Leukemia
,
Genetic Mechanisms and Molecular Markers of Neoplastic Transformation in Acute Myeloid Leukemia
,
Molecular Pathogenesis of Aggressive B-cell Lymphomas
,
Molecular Biology and Classification of Multiple Myeloma
,
Chronic Lymphocytic Leukemia
,
Molecular Biology of Chronic Myeloid Leukemia
,
Molecular Biology of Myelodysplastic Syndromes
,
Myeloproliferative Neoplasms
,
Part 2.Diagnostics and Monitoring of Therapy of Hemato-oncologic DeseasesFlow Cytometric Immunophenotyping as Diagnostic Tool of Hematopoietic Malignancies
,
Cytogenetics in Hematology
,
Monitoring of Minimal Residual Disease in Acute Lymphoblastic Leukemia
,
Gene Expression Profiling in Hematologic Malignancies
,
Part 3.Molecular Procedures Involved in Hematopoietic Stem Cell TransplantationAllogeneic Transplantation of Hematopoietic Stem Cells
,
Genetic Basis of Donor-Recipient Matching in Allogeneic Transplantation of Hematopoietic Stem Cells
,
Chimerism Following Allogeneic Transplantation of Hematopoietic Stem Cells
,
Peritransplantation Monitoring of Minimal Residual Disease in Acute Lymphoblastic Leukemia
,
Biobanks of Cellular Material
,
Part 4.Molecular Diagnostic ProtocolsBiobanking of Cellular Material
,
Isolation of Mononuclear Cells from Human Blood and Bone Marrow by Density Gradient Centrifugation
,
Molecular Techniques Commonly Used in Hemato-oncology
,
Genetic Methods of HLA Typing
,
Post-Transplant Chimerism Analysis Through STR-PCR and RQ-PCR
,
Analysis of Minimal Residual Disease with the Use of Rearrangements of Ig/TCR Genes Through RQ-PCR
,
Molecular Diagnostics of Acute Myeloblastic Leukemia
,
Assessment of the Presence and the Level of BCR-ABL Fusion Gene Expression and Mutational Status in ABL Kinase Domain
,
Studies of Rearrangements and Somatic Hypermutation of IGHV Genes in Chronic Lymphocytic Leukemia
,
Molecular Biology Methods in the Diagnosis of Multiple Myeloma
,
Molecular Methods in Myeloproliferative Neoplasms
,
Informed Consent for Participation in Research Project
Additional Edition:
ISBN 9783642294662
Additional Edition:
Buchausg. u.d.T. Molecular aspects of hematologic malignancies Berlin : Springer, 2012 ISBN 9783642294662
Additional Edition:
ISBN 3642294669
Language:
English
Keywords:
Leukämie
;
Malignes Lymphom
;
Molekulare Medizin
;
Aufsatzsammlung
DOI:
10.1007/978-3-642-29467-9
URL:
Volltext
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