Kooperativer Bibliotheksverbund

UID:

Format: XV, 830 S. : Ill., graph. Darst.

Edition: 2. ed.

ISBN: 1-55581-128-0

Note: Literaturangaben

Language: English

Subjects: Biology

Keywords: Biotechnologie ; Industrielle Mikrobiologie

UID:

Format: 211 S. : Ill., graph. Darst.

ISBN: 3-89308-220-4

Language: German

Subjects: Biology

Keywords: 1929-2008 Zähner, Hans ; Mikroorganismus ; Sekundärstoffwechsel ; Mikroorganismus ; Sekundärmetabolit ; 1929-2008 Zähner, Hans ; Aufsatzsammlung ; Bibliografie ; Konferenzschrift ; Konferenzschrift ; Bibliografie ; Aufsatzsammlung ; Bibliografie ; Konferenzschrift ; Aufsatzsammlung ; Bibliografie ; Konferenzschrift

URL: http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006932240&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA

URL: Inhaltsverzeichnis

URL: http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006932240&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA

UID:

Format: Online-Ressource (digital)

ISBN: 9781592599769

Series Statement: SpringerLink

Content: Although the natural product drug discovery programs of the large drug companies are now equaled by programs for the high throughput screening of synthetic compounds generated through combinatorial chemistry, natural compounds still hold great promise to overcome such problems as antibiotic resistance, the emergence of new diseases, the failure to conquer old diseases, and the toxicity of some contemporary medical products. In Natural Products: Drug Discovery and Therapeutic Medicine, a panel of recognized experts and leaders in the field discuss the past successes of natural products as medicines and review future possibilities arising from both conventional and new technologies. High-performance liquid chromatography profiling, combinatorial synthesis, genomics, proteomics, DNA shuffling, bioinformatics, and genetic manipulation all now make it possible to rapidly evaluate the activities of extracts as well as purified components derived from microbes, plants, and marine organisms. The authors apply these methods to new natural product drug discovery, to accessing microbial diversity, to investigating specific groups of products (Chinese herbal drugs, antitumor drugs from microbes and plants, terpenoids, and arsenic compounds), and to exploiting specific sources (the sea, rainforest, and endophytes). These new opportunities show how research and development trends in the pharmaceutical industry can advance to include both synthetic compounds and natural products, and how this paradigm shift can be more productive and efficacious. State-of-the-art and forward looking, Natural Products: Drug Discovery and Therapeutic Medicine will inspire industrial and academic researchers, practitioners, and developers to once again explore natural products as key sources for the many new drugs needed to solve still unmet medical needs.

Note: Includes bibliographical references and index

Additional Edition: ISBN 9781588293831

Additional Edition: Buchausg. u.d.T. Natural products Totowa, N.J. : Humana Press, 2005 ISBN 1588293831

Additional Edition: ISBN 1592599206

Language: English

Subjects: Chemistry/Pharmacy , Medicine

Keywords: Naturstoff ; Arzneimittelforschung ; Naturstoff ; Arzneimittelforschung

DOI: 10.1007/978-1-59259-976-9

URL: Volltext

URL: Volltext  (lizenzpflichtig)

URL: Einführung/Vorwort

URL: Kapitel

URL: Cover

URL: Inhaltsverzeichnis

UID:

Format: 1 online resource

ISBN: 0-12-803746-6

Content: Biotechnology of Microbial Enzymes: Production, Biocatalysis and Industrial Applications provides a complete survey of the latest innovations on microbial enzymes, highlighting biotechnological advances in their production and purification along with information on successful applications as biocatalysts in several chemical and industrial processes under mild and green conditions.Applications of microbial enzymes in food, feed, and pharmaceutical industries are given particular emphasis. The application of recombinant DNA technology within industrial fermentation and the production of enzymes over the last 20 years have produced a host of useful chemical and biochemical substances. The power of these technologies results in novel transformations, better enzymes, a wide variety of applications, and the unprecedented development of biocatalysts through the ongoing integration of molecular biology methodology, all of which is covered insightfully and in-depth within the book.Features research on microbial enzymes from basic science through application in multiple industry sectors for a comprehensive approachIncludes information on metabolic pathway engineering, metagenomic screening, microbial genomes, extremophiles, rational design, directed evolution, and moreProvides a holistic approach to the research of microbial enzymes

Note: Front Cover -- Biotechnology of Microbial Enzymes -- Copyright Page -- Dedication -- Contents -- List of Contributors -- Preface -- 1 Useful Microbial Enzymes-An Introduction -- 1.1 The Enzymes: A Class of Useful Biochemicals -- 1.2 Microbial Enzymes for Industry -- 1.3 Improvement of Enzymes -- 1.4 Discovery of New Enzymes -- 1.5 Concluding Remarks -- Acknowledgements -- References -- 2 Production, Purification, and Application of Microbial Enzymes -- 2.1 Introduction -- 2.2 Production of Microbial Enzymes -- 2.2.1 Enzyme Production in Industries -- 2.2.2 Industrial Enzyme Production Technology -- 2.2.2.1 Submerged Fermentation -- 2.2.2.2 Solid State Fermentation -- 2.3 Strain Improvements -- 2.3.1 Mutation -- 2.3.2 Recombinant DNA (rDNA) Technology -- 2.3.3 Protein Engineering -- 2.4 Downstream Processing/Enzyme Purification -- 2.5 Product Formulations -- 2.6 Global Enzyme Market Scenarios -- 2.7 Industrial Applications of Enzymes -- 2.7.1 Food Industry -- 2.7.1.1 Starch Industry -- 2.7.1.2 Baking Industry -- 2.7.1.3 Brewing Industry -- 2.7.1.4 Fruit Juice Industry -- 2.7.2 Textile Industry -- 2.7.3 Detergent Industry -- 2.7.4 Pulp and Paper Industry -- 2.7.5 Animal Feed Industry -- 2.7.6 Leather Industry -- 2.7.7 Biofuel From Biomass -- 2.7.8 Enzyme Applications in the Chemistry and Pharma Sectors -- 2.7.8.1 Speciality Enzymes -- 2.7.8.2 Enzymes in Personal Care Products -- 2.7.8.3 Enzymes in DNA-Technology -- 2.8 Concluding Remarks -- References -- 3 Solid State Fermentation for Production of Microbial Cellulases -- 3.1 Introduction -- 3.2 Solid State Fermentation (SSF) -- 3.2.1 Comparative Aspects of Solid State and Submerged Fermentations -- 3.2.2 Cellulase-Producing Microorganisms in SSF -- 3.2.3 Extraction of Microbial Cellulase in SSF -- 3.2.4 Measurement of Cellulase Activity in SSF -- 3.2.4.1 Filter Paper Activity (FPase). , 3.2.4.2 Carboxymethyl Cellulase Activity (CMCase) -- 3.2.4.3 Xylanase Activity -- 3.2.4.4 β-Glucosidase Activity -- 3.3 Lignocellulosic Residues/Wastes as Solid Substrates in SSF -- 3.4 Pretreatment of Agricultural Residues -- 3.4.1 Physical/Mechanical Pretreatments -- 3.4.1.1 Mechanical Comminution -- 3.4.1.2 Grinding/Milling/Chipping -- 3.4.2 Physico-Chemical Pretreatments -- 3.4.2.1 Steam Explosion (Autohydrolysis) -- 3.4.3 Chemical Pretreatments -- 3.4.4 Biological Pretreatment -- 3.5 Environmental Factors Affecting Microbial Cellulase Production in SSF -- 3.5.1 Water Activity/Moisture Content -- 3.5.2 Temperature -- 3.5.3 Mass Transfer Processes: Aeration and Nutrient Diffusion -- 3.5.3.1 Gas Diffusion -- 3.5.3.2 Nutrient Diffusion -- 3.5.4 Substrate Particle Size -- 3.5.5 Other Factors -- 3.6 Strategies to Improve Production of Microbial Cellulase -- 3.6.1 Metabolic Engineering and Strain Improvement -- 3.6.2 Recombinant Strategy (Heterologous Cellulase Expression) -- 3.6.2.1 Yeast Expression Systems -- 3.6.2.2 Bacterial Expression Systems -- 3.6.2.3 Plant Expression System -- 3.6.3 Mixed-Culture (Coculture) Systems -- 3.7 Fermenter (Bioreactor) Design for Cellulase Production in SSF -- 3.7.1 Tray Type Bioreactor -- 3.7.2 Rotary Drum Bioreactor -- 3.7.3 Packed Bed Bioreactor -- 3.7.4 Fluidized Bed Bioreactor -- 3.8 Biomass Conversion and Application of Microbial Cellulases -- 3.8.1 Textile Industry -- 3.8.2 Laundry and Detergents -- 3.8.3 Food and Animal Feed -- 3.8.4 Pulp and Paper Industry -- 3.8.5 Biofuels -- 3.9 Concluding Remarks -- Abbreviations -- References -- 4 Hyperthermophilic Subtilisin-Like Proteases From Thermococcus kodakarensis -- 4.1 Introduction -- 4.2 Two Subtilisin-Like Serine Proteases From Thermococcus kodakarensis KOD1 -- 4.3 Tk-Subtilisin -- 4.3.1 Ca2+-Dependent Maturation of Tk-Subtilisin. , 4.3.2 Crystal Structures of Tk-Subtilisin -- 4.3.3 Requirement of Ca2+-Binding Loop for Folding -- 4.3.4 Ca2+ Ion Requirements for Hyperstability -- 4.3.5 Role of Tkpro -- 4.3.6 Role of the Insertion Sequences -- 4.3.7 Cold-Adapted Maturation Through Tkpro Engineering -- 4.3.8 Degradation of PrPSc by Tk-Subtilisin -- 4.3.9 Tk-Subtilisin Pulse Proteolysis Experiments -- 4.4 Tk-SP -- 4.4.1 Maturation of Pro-Tk-SP -- 4.4.2 Crystal Structure of Pro-S359A* -- 4.4.3 Role of proN -- 4.4.4 Role of the C-Domain -- 4.4.5 PrPSc Degradation by Tk-SP -- 4.5 Concluding Remarks -- Acknowledgments -- Abbreviations -- References -- 5 Enzymes from Basidiomycetes-Peculiar and Efficient Tools for Biotechnology -- 5.1 Introduction -- 5.2 Brown and White Rot Fungi -- 5.3 Isolation and Laboratory Maintenance of Wood Rot Basidiomycetes -- 5.4 Basidiomycetes as Producers of Enzymes Involved in Degradation of Lignocellulose Biomass -- 5.4.1 Enzymes Involved in the Degradation of Cellulose and Hemicelluloses -- 5.4.2 Enzymes Involved in Lignin Degradation -- 5.5 Production of Ligninolytic Enzymes by Basidiomycetes: Screening and Production in Laboratory Scale -- 5.6 General Characteristics of the Main Ligninolytic Enzymes with Potential Biotechnological Applications -- 5.6.1 Laccases -- 5.6.2 Peroxidases -- 5.7 Industrial and Biotechnological Applications of Ligninolytic Enzymes from Basidiomycetes -- 5.7.1 Application of Ligninolytic Enzymes in Delignification of Vegetal Biomass and Biological Detoxification for Biofuel P ... -- 5.7.2 Application of Ligninolytic Enzymes in the Degradation of Xenobiotic Compounds -- 5.7.3 Application of Ligninolytic Enzymes in the Degradation of Textile Dyes -- 5.7.4 Application of Ligninolytic Enzymes in Pulp and Paper Industry -- 5.8 Concluding Remarks -- Acknowledgments -- References. , 6 Microbial Production and Molecular Engineering of Industrial Enzymes: Challenges and Strategies -- 6.1 Introduction -- 6.2 Strategies for Achieving High-Level Expression of Industrial Enzymes in Microorganisms -- 6.2.1 Strategies for High-Level Expression of Microbial Enzymes in E. coli -- 6.2.1.1 High-Level Expression of Enzymes by Transcriptional Regulation in E. coli -- 6.2.1.2 High-Level Expression of Enzymes by Translational Regulation in E. coli -- 6.2.1.3 Enhancement of the Expression of Enzymes by Different Protein Formations in E. coli -- 6.2.1.4 Improving Enzyme Production Yield by Fusion Proteins or Molecular Chaperones in E. coli -- 6.2.1.5 High-Level Expression of Enzymes by Codon Optimization in E. coli -- 6.2.1.6 Fermentation Optimization of Enzyme Production in E. coli -- 6.2.2 High-Level Expression of Microbial Enzymes in Bacilli -- 6.2.3 High-Level Expression of Microbial Enzymes in Lactic Acid Bacteria -- 6.2.4 High-Level Expression of Microbial Enzymes in Yeasts -- 6.2.4.1 High-Level Expression of Microbial Enzymes in P. pastoris -- 6.2.4.2 High-Level Expression of Microbial Enzymes in S. cerevisiae -- 6.2.4.3 High-Level Expression of Microbial Enzymes in Other Yeast Hosts -- 6.2.5 High-Level Expression of Microbial Enzymes in Filamentous Fungi -- 6.2.5.1 High-Level Expression of Microbial Enzymes in Aspergillus Species -- 6.2.5.2 High-Level Expression of Microbial Enzymes in Trichoderma Species -- 6.2.5.3 High-Level Expression of Microbial Enzymes in Other Filamentous Fungi Species -- 6.3 Molecular Engineering Strategies -- 6.3.1 Directed Evolution -- 6.3.2 Site-Directed Mutagenesis -- 6.3.3 Saturation Mutagenesis -- 6.3.4 Truncation -- 6.3.5 Fusion -- 6.4 Concluding Remarks -- References -- 7 Metagenomics and the Search for Industrial Enzymes -- 7.1 Introduction -- 7.2 The Dilemma Between Known, Engineered, or Novel Enzymes. , 7.3 Metagenomics and Its Application to Enzyme Research -- 7.4 Success Stories of Naïve and Direct Sequencing Screens for New Enzymes -- 7.5 Success Stories for Introducing Environmental Enzymes into the Market -- 7.6 Enzyme Search: Limitations of Metagenomics and Solutions -- 7.7 Concluding Remarks -- Acknowledgments -- References -- 8 The Pocket Manual of Directed Evolution: Tips and Tricks -- 8.1 Introduction -- 8.2 Methods to Generate DNA Diversity -- 8.2.1 Mutagenic Methods -- 8.2.1.1 Random Mutagenesis -- 8.2.1.2 Saturation Mutagenesis -- 8.2.2 DNA Recombination Methods -- 8.2.2.1 In Vitro Methods -- 8.2.2.1.1 Homology-Dependent Recombination Methods -- 8.2.2.1.2 Homology-Independent Recombination Methods -- 8.2.2.2 In Vivo Methods -- 8.3 Computational Tools -- 8.4 Functional Expression Systems -- 8.5 Mutant Library Exploration -- 8.5.1 Genetic Selection Methods -- 8.5.2 High-Throughput Screening (HTS) Assays -- 8.5.3 Ultrahigh-Throughput Screening Assays -- 8.6 Forthcoming Trends in Directed Evolution -- 8.7 Concluding Remarks -- Acknowledgments -- Abbreviations -- References -- 9 Insights into the Structure and Molecular Mechanisms of β-Lactam Synthesizing Enzymes in Fungi -- 9.1 Introduction -- 9.1.1 Penicillin and Cephalosporin Biosynthesis: A Brief Overview -- 9.1.2 Genes Involved in Penicillin Biosynthesis -- 9.2 ACV Synthetase -- 9.2.1 The ACV Assembly Line -- 9.2.2 The Cleavage Function of the Integrated Thioesterase Domain -- 9.2.3 The Quality Control (Proofreading) Role of the Thioesterase Domain -- 9.2.4 ACV Analog Dipeptides and Tripeptides Synthesized by the ACVS in Vitro -- 9.3 Isopenicillin N Synthase -- 9.3.1 Binding and Lack of Cyclization of the LLL-ACV -- 9.3.2 The Iron-Containing Active Center -- 9.3.3 The Crystal Structure of IPNS -- 9.3.4 Oxidase and Oxygenase Activities of IPNS. , 9.3.5 Recent Advances on the Cyclization Mechanism.

Additional Edition: ISBN 0-12-803725-3

Language: English

UID:

Format: XI, 466 S. : graph. Darst.

ISBN: 0-914826-72-7

Language: English

Subjects: Biology

Keywords: Biotechnologie ; Industrielle Mikrobiologie

UID:

Format: XVIII, 573 S. : , Ill.

ISBN: 0-8053-2451-8

Series Statement: Biotechnology series 6

Language: English

Subjects: Biology

Keywords: Industrielle Mikrobiologie

UID:

Format: XVIII, 573 S. , Ill., graph. Darst.

ISBN: 0409901652

Series Statement: Biotechnology 6

Note: Nebent.: Biology of industrial microorganisms / Arnold L. Demain ; Nadine A. Solomon

Language: Undetermined

Subjects: Biology

Keywords: Industrielle Mikrobiologie

UID:

Format: 25 cm

Series Statement: Handbook of experimental pharmacology 67

Language: English

UID:

Format: 429, A11 Seiten , Illustrationen

ISBN: 9781908230546

Content: "In this timely book, respected international experts summarize the most important research to provide an overview of the field [antibiotics]. Opening chapters define 'antibiotic', explain why we need new compounds, outline the applications of antibiotics, both old and new, and describe the producing microbes. These are followed by chapters that cover antibiotic resistance, toxicity, overuse, new antimicrobial sources, new targets, novel technologies for antibiotic discover (e.g. silent gene clusters), lantibiotics, natural antivirals, new macrolide derivatives, and antibiotics in the pipeline.This book is essential reading for everyone working in antimicrobial research in academia, biotechnology companies, and the pharmaceutical industry and a recommended volume for all microbiology libraries." --

Note: ContributorsPreface ; What is an antibiotic? , New sources of antibiotics: caves , Main applications of antibiotics , Microorganisms producing antibiotics , The need for new antibiotics , Tackling antibiotic resistance , Toxicity of antibacterial drugs , Overuse of antibiotics: non-medical applications , Endophytes as a potential source of new antibiotics , Antibiotics from microorganisms from hot springs/geysers , New targets for antibacterial compounds , Novel antimicrobial and other bioactive metabolites obtained from silent gene clusters , Combinatorial biosynthesis for antibiotic discover , Lantibiotics and other antibacterial peptides , Antiviral compounds of natural origin , New compounds with antibacterial activity ; Patricia Veiga-Crespo [and others] ; Use of antibiotic core structures to generate new and useful macrolide antibiotics , Antibiotics in the pipeline , Index ; Colour plate.

Additional Edition: ISBN 9781908230553

Language: English

Subjects: Chemistry/Pharmacy

Keywords: Antibiotikum ; Aufsatzsammlung

UID:

Series Statement: Handbook of experimental pharmacology ...

Language: English