UID:
almahu_9949251328002882
Format:
1 online resource (XII, 298 p. 53 illus., 40 illus. in color.)
Edition:
1st ed. 2016.
ISBN:
1-4939-3378-7
Series Statement:
Methods in Molecular Biology, 1402
Content:
This volume assembles a broad spectrum of methods used in long non-coding RNAs (lncRNA) research, ranging from computational annotation of lncRNA genes to molecular and cellular analyses of the function of individual lncRNA. Long Non-Coding RNAs: Methods and Protocols also discusses methods used to study circular RNAs and RNA splicing, as well as influential findings on lncRNA in human diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Long Non-Coding RNAs: Methods and Protocols is a must-have for molecular biologists, cell and developmental biologists, specialists who conduct disease-oriented research, and bioinformatics experts who seek a better understanding on lncRNA expression and function by computational analysis of the massive sequencing data that are rapidly accumulating in recent years.
Note:
Bibliographic Level Mode of Issuance: Monograph
,
LncRNA pulldown combined with mass spectrometry to identify the novel LncRNA-associated proteins /
,
Cross-linking immunoprecipitation and qPCR (CLIP-qPCR) analysis to map interactions between long noncoding RNAs and RNA-binding proteins /
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Characterization of long noncoding RNA-associated proteins by RNA-immunoprecipitation /
,
Isolation of protein complexes associated with long noncoding RNAs /
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Profiling long noncoding RNA expression using custom-designed microarray /
,
Long noncoding RNA expression profiling using arraystar LncRNA microarrays /
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Nuclear RNA isolation and sequencing /
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Targeted LncRNA sequencing with the SeqCap RNA enrichment system /
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ChIP-Seq : library preparation and sequencing /
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Stellaris® RNA fluorescence in situ hybridization for the simultaneous detection of immature and mature long noncoding RNAs in adherent cells /
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Simultaneous RNA-DNA FISH /
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Visualizing long noncoding RNAs on chromatin /
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Non-isotopic method for in situ LncRNA visualization and quantitation /
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Detection of long noncoding RNA expression by nonradioactive northern blots /
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Assessment of in vivo SiRNA delivery in cancer mouse models /
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Targeting long noncoding RNA with antisense oligonucleotide technology as cancer therapeutics /
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Characterization of circular RNAs /
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Methods for characterization of alternative RNA splicing /
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NONCODEv4 : annotation of noncoding RNAs with emphasis on long noncoding RNAs /
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Computational analysis of LncRNA from cDNA sequences /
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Analyzing MiRNA-LncRNA interactions /
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Long noncoding RNAs : an overview /
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English
Additional Edition:
ISBN 1-4939-3376-0
Language:
English
Keywords:
Laboratory Manuals
;
Laboratory Manuals
;
Laboratory Manuals
;
Laboratory Manuals
DOI:
10.1007/978-1-4939-3378-5
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