Kooperativer Bibliotheksverbund

UID:

Format: XV, 171 p. 41 illus., 17 illus. in color. , online resource.

Edition: 1st ed. 2023.

ISBN: 9789819927647

Series Statement: Peking University Linguistics Research, 7

Content: This book is a current and comprehensive study that incorporates constructional frameworks to inquire grammatical metaphor of modality (MM) for more adequate description and explanation of grammatical metaphor. It conducts semantic analysis of MM via a refined cognitive model and investigation of its lexicogrammatical forms from corpus-based constructional approach. The result is a reliable and systemic record of MM in real use, coupled with theoretical refinement from a cognitive perspective to illuminate its 'metaphoricity' motivation. The constructional approach and methodology exemplified in this book provide fresh insights, rich data, and a feasible solution for the adequate description and explanation of grammatical metaphor phenomena and the form-meaning interactive complexity in modality.

Note: Chapter 1. Introduction -- Chapter 2. Grammatical Metaphor and Metaphors of Modality -- Chapter 3. A new proposal for modelling metaphors of modality as constructions -- Chapter 4. The semantics of Modal Metaphors -- Chapter 5. The lexico grammatical forms of MM -- Chapter 6. The functional accounts of MM -- Chapter 7. Conclusions.

In: Springer Nature eBook

Additional Edition: Printed edition: ISBN 9789819927630

Additional Edition: Printed edition: ISBN 9789819927654

Additional Edition: Printed edition: ISBN 9789819927661

Language: English

DOI: 10.1007/978-981-99-2764-7

URL: https://doi.org/10.1007/978-981-99-2764-7

URL: Volltext  (URL des Erstveröffentlichers)

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Format: 41 S. : , graph. Darst.

Series Statement: Working paper series / National Bureau of Economic Research 8092

Language: English

URL: Volltext  (kostenfrei)

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Format: XIX, 315, III Seiten.

Note: Dissertation Humboldt-Universität zu Berlin 2019

Additional Edition: Erscheint auch als Online-Ausgabe Hu, Jian Haftung für Erfüllungsgehilfen nach deutschem und chinesischem Recht 10.18452/20003

Language: German

Subjects: Law

Keywords: Hochschulschrift ; Hochschulschrift

URL: Volltext  (kostenfrei)

URL: Volltext  (kostenfrei)

UID:

Format: 1 Online-Ressource (XV, 171 p. 41 illus., 17 illus. in color).

Edition: 1st ed. 2023

ISBN: 978-981-9927-64-7

Series Statement: Peking University Linguistics Research 7

Additional Edition: Erscheint auch als Druck-Ausgabe ISBN 978-981-9927-63-0

Additional Edition: Erscheint auch als Druck-Ausgabe ISBN 978-981-9927-65-4

Additional Edition: Erscheint auch als Druck-Ausgabe ISBN 978-981-9927-66-1

Language: English

Keywords: Grammatik ; Metapher ; Modalität

DOI: 10.1007/978-981-99-2764-7

URL: Volltext  (URL des Erstveröffentlichers)

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Format: 1 online resource (366 pages)

Edition: 1st ed.

ISBN: 0-323-95777-3 , 0-323-95776-5

Note: Front Cover -- Series page -- Methods in ENZYMOLOGY -- Copyright -- Contents -- Contributors -- Preface -- Chapter One: Considerations in production of the prokaryotic ZIP family transporters for structural and functional studiesProduction of the prokaryotic ZIP family transporters -- 1 Introduction -- 1.1 Phylogenetic relationships and overall topology of ZIP transporters -- 1.2 ZIP transporters in plants, yeast and humans -- 1.3 Structural and functional overview of ZIP transporters -- 2 Plasmids construction -- 2.1 Choosing a protein of interest -- 2.2 Expression hosts -- 2.3 Topology of a membrane protein and affinity tags -- 2.4 Cloning methods and codon usage -- 3 Expression screens -- 4 Detergents screens -- 5 Target protein purifications -- 6 Stability screens -- 7 Function assays -- 8 Summary -- Acknowledgements -- References -- Chapter Two: Expression, purification, crystallization of a ZIP metal transporter from Bordetella bronchiseptica (BbZIP) -- 1 Introduction -- 2 Overexpression of BbZIP -- 2.1 Considerations of host, construct, and candidate for production of a ZIP protein -- 2.2 Equipment -- 2.3 Materials -- 2.4 Procedure and expected results -- 2.4.1 Generation of the construct for BbZIP overexpression -- 2.4.2 Preparation of the LB media and the LBE-5052 autoinduction media -- 2.4.3 Expression of BbZIP -- 3 Purification of BbZIP -- 3.1 Considerations of BbZIP purification -- 3.2 Equipment -- 3.3 Materials -- 3.4 Procedure and expected results -- 3.4.1 Preparation of the membrane fraction of spheroplasts -- 3.4.2 Purification of BbZIP -- 4 Crystallization of BbZIP -- 4.1 Considerations of BbZIP Crystallization -- 4.2 Equipment -- 4.3 Materials -- 4.4 Procedure and expected results -- 4.4.1 Crystallization of BbZIP in LCP -- 4.4.2 Harvest and test shooting of the LCP crystals -- 4.4.3 Crystallization of BbZIP in DDM -- 5 Summary. , Acknowledgments -- References -- Chapter Three: Expression, purification, and crystallization of the extracellular domain of a mammalian ZIP4 -- 1 Introduction -- 2 Before you begin -- 3 Key resource table -- 4 Materials and equipment -- 5 Step-by-step method details -- 5.1 Expression of pZIP4-ECD -- 5.2 Protein purification -- 5.3 Protein crystallization -- 6 Expected outcomes -- 7 Discussion -- References -- Chapter Four: Methods to visualise zinc transporter proteins of the SLC39A family in cells -- 1 Introduction -- 2 General methods -- 3 Special treatment considerations and probe choice -- 4 Transfection -- 4.1 Equipment and reagents -- 4.2 Procedure -- 5 Western blotting -- 5.1 Equipment and reagents -- 5.2 Buffers -- 5.2.1 Procedure -- 5.2.1.1 Lysis (on ice, 2-4 °C) -- 6 Immunofluorescence -- 6.1 Equipment and reagents -- 6.2 Buffers -- 6.2.1 Procedure -- 6.2.1.1 Fixing and staining coverslips -- 7 Proximity ligation assay -- 7.1 Equipment and reagents -- 7.2 Procedure -- 8 Summary and conclusions -- Appendix 1 Recommended probes for visualisation of ZIP6, ZIP7 and ZIP10 -- References -- Chapter Five: A simple and highly reproducible method for the detection of erythrocyte membrane ZIP metal transporters by immunoblotting -- 1 Introduction -- 2 Isolation of erythrocyte membrane from mouse blood -- 2.1 Major equipment required -- 2.2 Preparation of reagents -- 2.3 Protocol for erythrocyte membrane isolation -- 3 Detection of ZIP proteins by immunoblotting method -- 3.1 Major equipment required -- 3.2 Preparation of reagents -- 3.3 Protocol for the detection of ZIP proteins by immunoblotting -- 3.4 Essential controls for immunoblotting -- 4 Discussion -- Institutional review board statement -- Conflicts of interest -- Funding -- CRediT authorship contribution statement -- Appendix A Supporting information -- References. , Chapter Six: Single-molecule quantification of the oligomeric state of ZIP transporters in mammalian cells with fluorescence correlation spectroscopySingle-molecule quantification of ZIP transporters -- 1 Introduction -- 2 General safety measures -- 3 Molecular design of fluorescent protein tagged gene fusions -- 3.1 Equipment -- 3.2 Reagents -- 3.3 Procedure -- 3.4 Notes -- 4 Mammalian cell culture -- 4.1 Equipment -- 4.2 Reagents -- 4.3 Procedure -- 4.4 Notes -- 5 Confocal microscopy set up -- 5.1 Equipment -- 5.2 Reagents -- 5.3 Procedure -- 6 Fluorescence correlation spectroscopy data analysis -- 6.1 Equipment -- 6.2 Procedure -- 6.3 Notes -- 7 Summary and conclusions -- Acknowledgments -- References -- Chapter Seven: Cell-based transport assay to study kinetics and substrate specificity of human ZIPsCell-based transport assay to study kinetics and substrate specificity of human ZIPs -- 1 Introduction -- 2 Determination of zinc ion transport kinetics of human ZIP4 using cell-based transport assay -- 2.1 General considerations of activity measurement of transporters -- 2.2 Equipment -- 2.3 Materials -- 2.4 Procedure and expected results -- 2.4.1 Plasmid construct and preparation -- 2.4.2 Cell culture and transfection -- 2.4.3 Western blot -- 2.4.4 Time course of Zn2+ transport by human ZIP4 -- 2.4.5 Dose-dependent assay of human ZIP4 and curve fitting -- 3 Comparison of substrate specificities of human ZIP8 and human ZIP4 using cell-based transport assay -- 3.1 Considerations of studying a transporter's activities toward multiple substrates -- 3.2 Equipment -- 3.3 Materials -- 3.4 Procedure and expected results -- 3.4.1 Simultaneous detection of 65Zn and 109Cd -- 3.4.2 Plasmid preparation, transfection, and western blot -- 3.4.3 Comparison of the substrate preference of human ZIP8 and human ZIP4 -- 4 Summary -- Acknowledgments -- References. , Chapter Eight: Assessing metal ion transporting activity of ZIPs: Intracellular zinc and iron detectionAssessing metal ion transporting activity of ZIPs -- 1 Introduction -- 2 Intracellular zinc detection -- 2.1 Metal content measurement with ICP-MS -- 2.1.1 Materials and equipment -- 2.1.2 Protocols -- 2.2 Measurement of ALP activity -- 2.2.1 Materials and equipment -- 2.2.2 Protocols -- 2.3 Zinpyr-1 staining for intracellular labile Zn2+ -- 2.3.1 Materials and equipment -- 2.3.2 Protocols -- 2.4 Measuring the transcription level of metallothioneins -- 2.4.1 Materials and equipment -- 2.4.2 Protocols -- 3 Intracellular iron detection -- 3.1 Aconitase activity assay -- 3.1.1 Materials and equipment -- 3.1.2 Protocols -- 3.2 Iron staining -- 3.2.1 Detection of ferric iron in tissues -- 3.2.2 Materials and equipment -- 3.2.3 Protocols -- 3.2.4 Ferric iron staining of ferritin in protein samples after electrophoresis separation -- 3.2.5 Materials and equipment -- 3.2.6 Protocols -- 3.3 Detection of intracellular ferrous iron with iron probes -- 3.3.1 Cytosolic labile iron pool (LIP) measurement -- 3.3.1.1 Materials and equipment -- 3.3.1.2 Protocols -- 3.3.2 Mitochondrial LIP measurement -- 3.3.2.1 Materials and equipment -- 3.3.2.2 Protocols -- 3.3.3 Golgi LIP measurement -- 3.3.3.1 Materials and equipment -- 3.3.3.2 Protocols -- 3.4 Ferrozine-based colorimetric assay -- 3.4.1 Materials and equipment -- 3.4.2 Protocols -- 4 Summary -- Acknowledgments -- References -- Chapter Nine: In vitro studies of manganese transport and homeostasisIn vitro studies of manganese transport and homeostasis -- 1 Introduction -- 2 General method and statistical analysis -- 3 Vector construction and site-directed mutagenesis of SLC39A8 -- 3.1 Equipment -- 3.2 Reagents -- 3.3 Procedure -- 3.3.1 Vector construction -- 3.3.2 Site-directed mutagenesis -- 3.4 Notes. , 4 Transient transfection of SLC39A8 and its mutants into HeLa cells -- 4.1 Equipment -- 4.2 Reagents -- 4.3 Procedure -- 4.4 Notes -- 5 Measurement of total Mn concentration -- 5.1 Equipment -- 5.2 Reagents -- 5.3 Procedure -- 5.4 Notes -- 6 54Mn uptake assay -- 6.1 Equipment -- 6.2 Reagents -- 6.3 Procedure -- 6.4 Notes -- 7 GPP130 degradation assay -- 7.1 Equipment -- 7.2 Reagents -- 7.3 Procedure -- 7.3.1 Cell treatment and lysis -- 7.3.2 Immunoblot detection of GPP130 -- 7.4 Notes -- 8 Immunofluorescence imaging -- 8.1 Equipment -- 8.2 Reagents -- 8.3 Procedure -- 8.4 Notes -- 9 Summary and conclusions -- Acknowledgments -- References -- Chapter Ten: Simple in vitro method to evaluate ZIP zinc transport ability through zinc transporter 1 and metallothionein expression measurementsMethod to evaluate ZIP zinc transport ability -- 1 Introduction -- 2 Evaluation of Zn transport of transfected ZIPs by analyzing ZNT1 expression using immunofluorescence staining -- 2.1 Equipment -- 2.2 Reagents -- 2.3 Procedure -- 3 Evaluation of Zn transport of ZIPs by analyzing ZNT1 expression with immunoblot analysis -- 3.1 Establishment of Flp-In T-REx MDCK cells -- 3.2 Equipment -- 3.3 Reagents -- 3.4 Procedure -- 3.4.1 Establishment of clones of Flp-In T-REx MDCK cells stably expressing ZIPs -- 3.4.2 Evaluation of ZIPs-driven ZNT1 expression changes by immunoblot analysis -- 4 Use of ZNT1 expression to monitor the decreased Zn levels mediated by loss of ZIP expression -- 4.1 Equipment and reagents -- 4.2 Procedure -- 5 Monitoring ZIPs-driven ZNT1 expression in polarized cells -- 5.1 Equipment and reagents -- 5.2 Procedure -- 6 Evaluation of the Zn transport ability of ZIPs through analyzing MT expression -- 6.1 Equipment and regents -- 6.2 Procedure. , 7 In vitro screening through evaluating dietary components enhancing ZIP4-mediated Zn uptake: Application of the ZIP4-mediated MT expression response.

Language: English

UID:

Format: XIV, 399 S.

ISBN: 0-7923-9873-4

Series Statement: The Kluwer international series in engineering and computer science 392

Language: English

Subjects: Computer Science , Mathematics

Keywords: Monte-Carlo-Simulation ; Störungstheorie ; Gradientenverfahren

URL: Inhaltsverzeichnis

URL: Inhaltsverzeichnis

URL: Inhaltstext

URL: http://www.loc.gov/catdir/enhancements/fy0820/96054595-d.html

URL: http://www.loc.gov/catdir/enhancements/fy0820/96054595-t.html

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Format: 390 S.

Original writing title: 生存的哲学

Original writing publisher: 长沙 : 湖南人民出版社

ISBN: 7217003261

Uniform Title: Living philosophy

Content: '生存的哲学' 是指对生死, 价值, 宗教, 劳动和自由等问题的哲学探讨. 本书介绍了世界上一些伟大的思想家对生存诸问题的看法

Note: 附录: 东方思想 , SBB-PK Berlin

Language: Chinese

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Format: 1 Online-Ressource (XV, 399 p)

ISBN: 9781461562931

Series Statement: The Springer International Series in Engineering and Computer Science, Discrete Event Dynamic Systems 392

Content: Conditional Monte Carlo: Gradient Estimation and Optimization Applications deals with various gradient estimation techniques of perturbation analysis based on the use of conditional expectation. The primary setting is discrete-event stochastic simulation. This book presents applications to queueing and inventory, and to other diverse areas such as financial derivatives, pricing and statistical quality control. To researchers already in the area, this book offers a unified perspective and adequately summarizes the state of the art. To researchers new to the area, this book offers a more systematic and accessible means of understanding the techniques without having to scour through the immense literature and learn a new set of notation with each paper. To practitioners, this book provides a number of diverse application areas that makes the intuition accessible without having to fully commit to understanding all the theoretical niceties. In sum, the objectives of this monograph are two-fold: to bring together many of the interesting developments in perturbation analysis based on conditioning under a more unified framework, and to illustrate the diversity of applications to which these techniques can be applied. Conditional Monte Carlo: Gradient Estimation and Optimization Applications is suitable as a secondary text for graduate level courses on stochastic simulations, and as a reference for researchers and practitioners in industry

Additional Edition: Erscheint auch als Druck-Ausgabe ISBN 9781461378891

Language: English

Keywords: Monte-Carlo-Simulation ; Störungstheorie ; Gradientenverfahren

DOI: 10.1007/978-1-4615-6293-1

URL: Volltext  (URL des Erstveröffentlichers)

UID:

Format: S. 463-579

Original writing person/organisation: 胡健国

ISBN: 754040423X

In: 湖南省戏曲研究所, hu nan di fang ju zhong zhi cong shu, chang sha, 1989, (1989), 5, 754040423X

In: year:1989

In: number:5

Language: Chinese

UID:

Format: S. 615-720

Original writing person/organisation: 胡健国

ISBN: 7540405988

In: 湖南省戏曲研究所, hu nan di fang ju zhong zhi cong shu, chang sha, 1990, (1990), 5, 7540405988

In: year:1990

In: number:5

Language: Chinese