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Format: X, 37 p. 7 illus., 6 illus. in color. , online resource.

ISBN: 9783319265902

Content: This book offers effective, low-cost and user-friendly protocols for the pre-field selection of salt-tolerant mutants in cereal crops. It presents simple methods for measuring soil salinity, including soil sampling and the analysis of water-soluble salts, and describes a detailed, but simple, screening test for salt tolerance in rice, wheat and barley seedlings, which uses hydroponics. The protocols are devised for use by plant breeders and can be easily accommodated into breeding practice. .

Note: Introduction -- Objectives -- Protocol for measuring soil salinity -- Protocol for screening for salt tolerance in rice -- Protocol for screening for salt tolerance in barley and wheat.  .

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Language: English

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DOI: 10.1007/978-3-319-26590-2

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Format: 1 Online-Ressource.

ISBN: 978-3-662-67273-0

Additional Edition: Erscheint auch als Druck-Ausgabe, Hardcover ISBN 978-3-662-67272-3

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Language: English

DOI: 10.1007/978-3-662-67273-0

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Format: 1 Online-Ressource.

ISBN: 978-3-662-67273-0

Additional Edition: Erscheint auch als Druck-Ausgabe, Hardcover ISBN 978-3-662-67272-3

Additional Edition: Erscheint auch als Druck-Ausgabe, Paperback ISBN 978-3-662-67275-4

Language: English

DOI: 10.1007/978-3-662-67273-0

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Format: 1 online resource (42 pages)

ISBN: 9783319162591

Note: Intro -- Foreword -- Acknowledgements -- Contents -- Chapter 1: Introduction -- 1.1 Background -- 1.2 Methods Used to Isolate Genomic DNA from Plant Tissues -- 1.3 Methods for the Discovery and Characterization of Induced and Natural Nucleotide Variation in Plant Genomes -- References -- Chapter 2: Health and Safety Considerations -- 2.1 Guidelines -- 2.2 Preparation of a Home-Made Chemical Spill Kit -- Reference -- Chapter 3: Sample Collection and Storage -- 3.1 Background -- 3.2 Materials -- 3.3 Methods -- References -- Chapter 4: Low-Cost DNA Extraction -- 4.1 Materials -- 4.2 Methods -- 4.2.1 Preparation of Silica Powder DNA Binding Solution -- 4.2.2 Low-Cost Extraction of Genomic DNA -- 4.3 Alternative Buffers for DNA Extraction -- Reference -- Chapter 5: PCR Amplification for Low-Cost Mutation Discovery -- 5.1 Materials -- 5.2 Methods -- References -- Chapter 6: Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- 6.1 Materials -- 6.2 Methods -- Reference -- Chapter 7: Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes from Common Weedy Pl... -- 7.1 Materials -- 7.2 Methods -- 7.2.1 Enzyme Extraction -- 7.2.2 Concentration of Enzymes Using Amicon Ultra 10 kDa MWCO Centrifugal Filter Devices (for 0.5 ml Starting Volume -- in 1.5-m... -- 7.2.3 Test of Mismatch Cleavage Activity -- Reference -- Chapter 8: Example Data -- 8.1 Quality of Genomic DNA Obtained by Silica Powder-Based DNA Extraction Method -- 8.2 Quality of Genomic DNA Obtained by Silica Powder-Based DNA Extraction Method Using Alternative Buffers -- 8.2.1 Summary -- 8.3 Example of PCR Products Using TILLING Primers with Source Genomic DNA from a Commercial Kit and Low-Cost Silica Method -- 8.4 Example of Low-Cost Agarose Gel-Based TILLING Assays for the Discovery of Induced Point Mutations. , 8.5 Example of Enzyme Activity Recovered from Weeds Compared to Crude Celery Juice Extract -- 8.5.1 Summary -- References -- Chapter 9: Conclusions.

Additional Edition: Print version: Till, Bradley J. Low-Cost Methods for Molecular Characterization of Mutant Plants Cham : Springer International Publishing AG,c2015 ISBN 9783319162584

Language: English

Keywords: Electronic books. ; Electronic books. ; Electronic books.

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ISBN: 9783662649152

Additional Edition: Erscheint auch als Druck-Ausgabe, Hardcover ISBN 978-3-662-64914-5

Additional Edition: Erscheint auch als Druck-Ausgabe, Paperback ISBN 978-3-662-64917-6

Language: English

DOI: 10.1007/978-3-662-64915-2

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Format: 1 Online-Ressource

ISBN: 9783662672730

Additional Edition: Erscheint auch als Druck-Ausgabe, Hardcover ISBN 978-3-662-67272-3

Additional Edition: Erscheint auch als Druck-Ausgabe, Paperback ISBN 978-3-662-67275-4

Language: English

DOI: 10.1007/978-3-662-67273-0

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UID:

Format: 1 online resource (343 pages)

ISBN: 9783319450216

Note: Intro -- Preface -- Acknowledgements -- Contents -- Chapter Reviewers -- Contributors -- Part I: Introduction -- Chapter 1: Mutagenesis for Crop Breeding and Functional Genomics -- 1.1 Inducing Genetic Variation -- 1.1.1 Practical Considerations in Induced Crop Mutagenesis -- 1.1.2 Developing Crop Varieties Using Induced Mutations -- 1.1.3 Elite Crop Varieties Developed Through Induced Mutations -- 1.2 Phenotypic Screening -- 1.2.1 Phenotypic Traits Developed Through Plant Mutation Breeding -- 1.3 Genotypic Screening of Mutant Plants -- 1.3.1 Genotypic Methods -- 1.3.1.1 Lower-Cost Mutation Discovery and Genotyping Methods -- 1.3.1.2 Higher-Throughput Genotyping and Mutation Discovery Methods -- 1.3.1.3 Cloning Mutant Alleles Causative for Improved Traits -- 1.4 Conclusion -- References -- Part II: Mutation Induction and Chimera Dissociation -- Chapter 2: Chemical and Physical Mutagenesis in Jatropha curcas -- 2.1 Introduction -- 2.2 Materials -- 2.2.1 In Vivo Material -- 2.2.2 In Vitro Material -- 2.2.3 Mutagenesis by Chemical Agents (See Note 2) -- 2.2.4 Mutagenesis by Physical Agents -- 2.3 Methods -- 2.3.1 In Vivo Material -- 2.3.2 In Vitro Material -- 2.3.3 Mutagenesis by Chemical Agents -- 2.3.3.1 EMS Mutagenesis of In Vivo Material (See Note 10) -- 2.3.3.2 EMS Mutagenesis of In Vitro Material (See Notes 10 and 20 and Fig. 2.2) -- 2.3.4 Mutagenesis by Physical Agents -- 2.3.4.1 Gamma Irradiation of In Vivo Material (See Notes 21-22) -- 2.3.4.2 Gamma Irradiation of In Vitro Material -- 2.3.4.3 X-Rays (See Note 32, Fig. 2.4) -- 2.4 Further Analyses -- 2.5 Notes -- References -- Chapter 3: Chemical Mutagenesis and Chimera Dissolution in Vegetatively Propagated Banana -- 3.1 Introduction -- 3.2 Materials -- 3.2.1 Culture Medium (S-27) -- 3.2.2 Chemical Toxicity Test -- 3.2.3 Calculation of Growth Reduction (GR) -- 3.2.4 Bulk Mutagenesis. , 3.2.5 Chimera Dissolution -- 3.3 Methods -- 3.3.1 Preparation of Liquid Culture Medium -- 3.3.2 Preparation of Solid Culture Medium -- 3.3.3 Chemical Toxicity Test -- 3.3.4 Calculation of Growth Reduction (GR) -- 3.3.5 Bulk Mutagenesis -- 3.3.6 Chimera Dissolution -- 3.4 Notes -- References -- Chapter 4: Mutation Induction Using Gamma Irradiation and Embryogenic Cell Suspensions in Plantain (Musa spp.) -- 4.1 Introduction -- 4.1.1 Somatic Embryogenesis in Musa spp. -- 4.1.2 Mutation Induction in Musa spp. -- 4.2 Materials -- 4.2.1 Explant Preparation: Shoot-Tip Establishment and Multiplication -- 4.2.2 Culture Medium and Incubation Materials -- 4.2.3 Acclimatization -- 4.2.4 Mutation Induction Using Gamma Irradiation -- 4.3 Methods -- 4.3.1 Explant Preparation: Shoot-Tip Establishment and Multiplication -- 4.3.1.1 Shoot-Tip Establishment -- 4.3.1.2 Shoot-Tip Multiplication -- 4.3.2 Protocol for Plant Regeneration via Somatic Embryogenesis -- 4.3.2.1 Callus Formation with Embryogenic Structures -- 4.3.2.2 Establishment and Multiplication of Embryogenic Cell Suspensions -- 4.3.2.3 Formation of Somatic Embryos -- 4.3.2.4 Maturation of Somatic Embryos -- 4.3.2.5 Germination of Somatic Embryos -- 4.3.2.6 Acclimatization Phase of Somatic Embryos, Conversion into Plants -- 4.3.3 Mutation Induction Using Gamma Irradiation -- 4.4 Notes -- References -- Chapter 5: Optimisation of Somatic Embryogenesis in Cassava -- 5.1 Introduction -- 5.1.1 Production of Cyclic Embryos -- 5.1.2 Influence of Growth Regulators on Primary Embryo Induction -- 5.1.3 Somatic Embryo Conversion into Plants -- 5.2 Materials -- 5.2.1 Chemicals and Equipment -- 5.2.2 Culture Media -- 5.2.3 Shoot Initiation Medium (see Notes 2-4) -- 5.2.4 Embryo Initiation Medium -- 5.2.5 Embryo Maturation Medium -- 5.2.6 Somatic Embryo Conversion Medium -- 5.3 Methods. , 5.3.1 Preparation of Shoot Initiation Medium -- 5.3.2 Collection and Sterilisation of Donor Plants -- 5.3.3 Sterilisation and Culture for Shoot Initiation -- 5.3.4 Culture Incubation -- 5.3.5 Initiation of Primary Somatic Embryos -- 5.3.6 Cyclic Embryo Initiation and Production -- 5.3.7 Abscisic Acid Effect on Conversion of Somatic Embryos into Plant -- 5.3.8 Desiccation of Embryos for Plant Conversion -- 5.4 Notes -- 5.5 Conclusion -- References -- Chapter 6: Creation of a TILLING Population in Barley After Chemical Mutagenesis with Sodium Azide and MNU -- 6.1 Introduction -- 6.2 Materials -- 6.2.1 Mutagenesis -- 6.2.2 Handling of Mutated Population -- 6.2.3 DNA Isolation -- 6.2.4 Creation of a Database -- 6.3 Methods -- 6.3.1 Mutagenesis -- 6.3.1.1 General Remarks -- 6.3.1.2 Mutagenic treatment -- 6.3.1.3 Evaluation of a Critical Dose of Mutagens -- 6.3.2 Handling of the Mutated Generations and the Basic Phenotyping of M2 Plants and M3 Lines -- 6.3.3 DNA Isolation: Creating the M2 DNA Library -- 6.3.3.1 Isolate DNA from the M2 Plants According to the Modified Micro-CTAB Method (Doyle and Doyle 1987 and see Note 19) -- 6.3.3.2 Prepare Pools of DNA to Identify Plants Carrying Mutations Within the Gene of Interest -- 6.3.4 Creation of a Database -- 6.3.4.1 General Information -- 6.3.4.2 Creation of a Database: An Example -- Notes -- References -- Chapter 7: Site-Directed Mutagenesis in Barley by Expression of TALE Nuclease in Embryogenic Pollen -- 7.1 Introduction -- 7.1.1 Site-Directed Mutagenesis in Plants -- 7.1.2 Haploid Technology -- 7.2 Materials -- 7.2.1 Donor Plants -- 7.2.2 Stock Solutions and Culture Media -- 7.2.2.1 Stock Solutions -- 7.2.2.2 Medium for Agrobacterium Tumefaciens -- 7.2.2.3 Media for Plant Cell Culture -- 7.2.3 Materials for the Isolation of Embryogenic Pollen -- 7.2.4 Materials for Agrobacterium-Mediated Transformation. , 7.2.5 Materials for the Analysis of Transgenic Plants -- 7.2.5.1 Ploidy Determination and Colchicine-Induced Whole Genome Duplication -- 7.2.5.2 Molecular Analyses -- DNA Isolation, PCR, and DNA Gel Blot Analysis -- RNA Isolation and Reverse Transcriptase Reaction -- 7.3 Methods -- 7.3.1 Vector Construction and Bacterial Strains -- 7.3.2 Growth of Donor Plants -- 7.3.3 Isolation of Immature Pollen -- 7.3.3.1 Spike Pretreatment -- 7.3.3.2 Isolation, Purification, and Pre-cultivation of Immature Pollen -- 7.3.4 Agrobacterium-Mediated Gene Transfer to Embryogenic Pollen -- 7.3.4.1 Preparation of A. tumefaciens Stocks -- 7.3.4.2 Cocultivation of Embryogenic Pollen Cultures and A. tumefaciens -- 7.3.5 Regeneration of Transgenics -- 7.3.6 Analysis of Putative Transgenic Plants -- 7.3.6.1 Ploidy Determination and Colchicine-Induced Whole Genome Duplication -- 7.3.6.2 Molecular Analyses -- DNA Isolation, PCR, and DNA Gel Blot Analysis -- RNA Isolation and Reverse Transcriptase Reaction -- 7.4 Notes -- References -- Chapter 8: Doubled Haploidy as a Tool for Chimaera Dissolution of TALEN-Induced Mutations in Barley -- 8.1 Introduction -- 8.1.1 Generation of Primary Mutants by Cross-Combination of Parental Lines Carrying Complementary Single TALEN Units -- 8.1.2 Chimaerism Upon TALEN-Induced Targeted Mutagenesis -- 8.1.3 Haploid Technology -- 8.2 Materials -- 8.2.1 Growth of Parental Lines and Primary Mutant Plants -- 8.2.2 Stock Solutions and Culture Media -- 8.2.2.1 Solutions for Isolation, Purification and Induction of Embryogenic Development of Immature Pollen -- 8.2.2.2 Nutrient Media for Embryogenic Pollen Culture and Plant Regeneration -- 8.2.3 Materials for the Isolation of Embryogenic Pollen Culture and Plant Regeneration -- 8.2.4 Materials for the Molecular Analyses of Pollen-Derived Plants. , 8.2.5 Materials for Ploidy Determination and Colchicine-Induced Whole-Genome Duplication -- 8.3 Methods -- 8.3.1 Growth of Primary Mutant Plants -- 8.3.2 Crossing of Pairs of Complementary Single TALEN Plants and Analysis of Hybrid Plants -- 8.3.3 Spike Preparation -- 8.3.4 Isolation, Purification and Inductive Treatment of Immature Pollen -- 8.3.5 Regeneration of Pollen-Derived Plants -- 8.3.6 Analysis of Pollen-Derived Plants -- 8.3.6.1 Ploidy Determination -- 8.3.6.2 DNA Isolation, PCR and Sequencing -- 8.3.6.3 Mutant Comparison -- 8.4 Notes -- References -- Part III: Phenotypic Screening -- Chapter 9: Field Evaluation of Mutagenized Rice Material -- 9.1 Introduction -- 9.2 Materials -- 9.2.1 Plot Design -- 9.2.2 Field Preparation and Planting -- 9.2.3 Rice Culture -- 9.2.4 Field Observations and Trait Evaluation -- 9.3 Methods -- 9.3.1 Plot Design -- 9.3.2 Field Preparation and Planting -- 9.3.3 Rice Culture -- 9.3.4 Field Observations and Trait Evaluation -- 9.4 Notes -- References -- Chapter 10: Root Phenotyping Pipeline for Cereal Plants -- 10.1 Introduction -- 10.1.1 Issue of Root Phenotyping -- 10.1.2 Root Phenotyping of Cereal Plants -- 10.1.3 Proposed Root Phenotyping Pipeline -- 10.2 Materials -- 10.2.1 Design of a Plant Growth System -- 10.2.2 Root Scanning Setup -- 10.3 Methods -- 10.3.1 Preparation of Culture Media -- 10.3.2 Controlling the System and Monitoring the Medium Parameters -- 10.3.3 Experiment Preparation and Maintenance -- 10.3.4 Medium Exchange -- 10.3.5 Experiment Termination and Root System Cleaning -- 10.3.6 Root System Analysis Using WinRHIZO System -- 10.3.7 Root Image Analysis -- 10.4 Notes -- References -- Chapter 11: Breeding New Aromatic Rice with High Iron Using Gamma Radiation and Hybridization -- 11.1 Introduction -- 11.2 Materials -- 11.3 Methods -- 11.3.1 Preparing a Mutant Population. , 11.3.2 Phenotypic Analysis of Aroma.

Additional Edition: Print version: Jankowicz-Cieslak, Joanna Biotechnologies for Plant Mutation Breeding Cham : Springer International Publishing AG,c2016 ISBN 9783319450193

Language: English

Keywords: Electronic books. ; Electronic books. ; Electronic books. ; Electronic books.

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Format: 1 online resource (xx, 314 pages) : , illustrations (some color)

Edition: 1st ed. 2023.

ISBN: 3-662-67273-1

Content: This open-access book presents essential concepts and new, illustrated methods for mutation-assisted breeding of Coffea arabica L. (Arabica), one of the world's most important cash crops and beverages. Arabica coffee accounts for about 60% of the world's coffee production. Coffee leaf rust (CLR), caused by the fungus Hemileia vastatrix is the major disease affecting Arabica coffee resulting in losses of over $1 billion annually. The geographical distribution of CLR is expanding due to climate change. Moreover, the genetic improvement of Arabica coffee is constrained due to its very narrow genetic base. This protocol book covers practical methods to enhance genetic diversity in Arabica coffee through induced mutagenesis and for screening for resistance to CLR. Current breeding approaches, challenges, and opportunities for Arabica coffee improvement are briefly reviewed and a survey of common coffee diseases with emphasis on CLR is presented. Based on latest advances in science and technology, this book includes novel methods for single-cell mutagenesis using in vitro cell and tissue culture techniques and for genome-wide screening of induced mutations using genomics tools. Each protocol chapter provides step-by-step illustrated methods supported by example results. Given the impact of recent CLR epidemics on Arabica coffee production in Latin America, the book is intended to serve as a timely reference and guide for students and researchers in the agricultural sciences, plant pathologists and breeders, as well as growers and end-users interested in producing novel coffee genotypes for genetic studies, breeding, and commercial applications. .

Note: Introduction coffee breeding and challenges -- Choice of materials for mutation induction in arabica coffee -- Improved in-vitro establishment and germination of Coffea arabica seed -- Induced mutagenesis in coffee (Coffea arabica L.) using chemical agents -- Mutation induction using gamma irradiation and high frequency embryogenic callus from coffee (Coffea arabica) -- Chemical mutagenesis of Coffea arabica mature seed using EMS -- Physical mutagenesis of coffee seeds -- In-vitro regeneration of Coffea arabica var. Venecia through somatic embryogenesis -- Protocol on mutation induction in Coffea arabica using in vivo grafting and cuttings -- Protocol on mutation induction in coffee using in vitro tissue cultures -- Screening for resistance to coffee leaf rust -- Protocol to send samples of coffee leaf rust to CIFC -- Coffee leaf rust (Hemileia vastatrix) inoculation and evaluation under laboratory conditions -- Development of a PCR-Based Molecular Detection -- Technique for the Early Diagnosis of Coffee Leaf -- Rust Caused by Hemileia vastatrix -- Protocols for chromosome preparations: molecular cytogenetics and studying genome organization in coffee. .

Additional Edition: ISBN 3-662-67272-3

Language: English

DOI: 10.1007/978-3-662-67273-0

UID:

Format: 1 Online-Ressource (xx, 340 Seiten) , Diagramme

ISBN: 9783319450216

Note: Open Access

Additional Edition: Erscheint auch als Druck-Ausgabe ISBN 978-3-319-45019-3

Language: English

Keywords: Pflanzenzüchtung ; Biotechnologie ; Mutationszüchtung

DOI: 10.1007/978-3-319-45021-6

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UID:

Format: 1 online resource (X, 35 p. 9 illus., 3 illus. in color.)

Edition: 1st ed. 2015.

ISBN: 3-319-16259-4

Content: This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.

Note: Bibliographic Level Mode of Issuance: Monograph , Introduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions.  . , English

Additional Edition: ISBN 3-319-16258-6

Language: English

DOI: 10.1007/978-3-319-16259-1