Kooperativer Bibliotheksverbund

UID:

Format: 1 online resource (XI, 335 p.)

Edition: 1st ed. 2009.

ISBN: 1-60327-977-6

Series Statement: Methods in Molecular Biology, 546

Content: In the last 20 years, research activity using the zebrafish Danio rerio has increased dramatically, due in part to the ease of breeding and raising them, their genetic tractability, embryonic accessibility, and their imaging potential. In Zebrafish: Methods and Protocols, expert researchers provide a collection of experimental techniques for the genetic modification, manipulation, and in vivo live imaging of zebrafish. This volume collects sophisticated and practical techniques, including several not yet widely known, from laboratories around the world. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Zebrafish: Methods and Protocols seeks to aid and inspire scientists utilizing this versatile animal model in their research.

Note: Bibliographic Level Mode of Issuance: Monograph , Mutagenesis and Mutants -- Highly Efficient ENU Mutagenesis in Zebrafish -- Production of Pseudotyped Retrovirus and the Generation of Proviral Transgenic Zebrafish -- Undertaking a Successful Gynogenetic Haploid Screen in Zebrafish -- Cryopreservation and In Vitro Fertilization at the Zebrafish International Resource Center -- Transgenesis -- Transient and Stable Transgenesis Using Tol2 Transposon Vectors -- Analysis of Genes and Genome by the Tol2-Mediated Gene and Enhancer Trap Methods -- Bacterial Artificial Chromosome Transgenesis for Zebrafish -- Simple and Efficient Transgenesis with Meganuclease Constructs in Zebrafish -- Tissue-Specific Manipulations -- Nitroreductase-Mediated Cell Ablation in Transgenic Zebrafish Embryos -- Focal Electroporation in Zebrafish Embryos and Larvae -- Tissue Micromanipulation in Zebrafish Embryos -- Analyzing Gene Expression -- Zebrafish Spotted-Microarray for Genome-Wide Expression Profiling Experiments. Part I: Array Printing and Hybridization -- Zebrafish Spotted-Microarray for Genome-Wide Expression Profiling Experiments: Data Acquisition and Analysis -- Validating microRNA Target Transcripts Using Zebrafish Assays -- Imaging -- Multiple Embryo Time-Lapse Imaging of Zebrafish Development -- Live Cell Imaging of Zebrafish Leukocytes -- Imaging Zebrafish Embryos by Two-Photon Excitation Time-Lapse Microscopy -- Morphological Analysis of the Zebrafish Digestive System -- In Toto Imaging of Embryogenesis with Confocal Time-Lapse Microscopy. , English

Additional Edition: ISBN 1-60327-976-8

Language: English

DOI: 10.1007/978-1-60327-977-2

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Format: Bildformat: Anamorphic 1.78:1 ; PAL

Note: Ländercode: 2 , Orig.: Japan, 2004 , Japan. mit engl. Untertiteln

In: Three ... Extremes : [DVD Video], [London], 2006, (2006)

Language: Japanese

Author information: Miike, Takashi

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Format: 2 DVD (ca. 126/95 Min.) , Tonformat: DD/5.1 , NTSC

Note: Extras: Disc 1: Audio commentary with Miike Takashi. Disc 2: Full-length feature "Dumplings". Making of Dumplings. , Japan. mit engl. Untertiteln

Language: Japanese

Author information: Miike, Takashi

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Format: Getr. Zählg. , Ill.

Series Statement: Yurashia bunkashi sensho 7

Language: Japanese

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Format: xii, 373 Seiten , Illustrationen

Edition: Second edition

ISBN: 9781493937691

Series Statement: Methods in molecular biology 1451

Content: Annotation, This second edition details new emerging areas of zebrafish research focusing on genetics and genomics, techniques for developing and analysing zebrafish disease models, and methods for neuroscience

Note: Includes bibliographical references and index , Part I. Genetics and genomics. Chemical screening in zebrafish , Chemical screening in zebrafish , Part II. Disease models and mechanism. Zebrafish as a model for the study of solid malignancies , Part III. Neuroscience. Targeted electroporation in embryonic, larval, and adult zebrafish , TALEN-mediated mutagenesis and genome editing , Homology-independent integration of plasmid DNA into the zebrafish genome , Detection of multiple genome modifications induced by the CRISPR/Cas9 system , Generation of targeted genomic deletions through CRISPR/Cas system in zebrafish , Testing of cis-regulatory elements by targeted transgene integration in zebrafish using PhiC31 integrase , Fluorescence-activated cell sorting and gene expression profiling of GFP-positive cells from transgenic zebrafish lines , Construction of the inbred strain , Zebrafish as a model for the study of solid malignancies , Melanoma regression and recurrence in zebrafish , Imaging of human cancer cell proliferation, invasion, and micrometastasis in a zebrafish xenogeneic engraftment model , Modeling leukemogenesis in the zebrafish using genetic and xenograft models , Enumerating hematopoietic stem and progenitor cells in zebrafish embryos , Live imaging of host-pathogen interactions in zebrafish larvae , Methodologies for inducing cardiac injury and assaying regeneration in adult zebrafish , Studying lipid metabolism and transport during zebrafish development , Targeted electroporation in embryonic, larval, and adult zebrafish , Studying axonal regeneration by laser microsurgery and high-resolution videomicroscopy , In vivo whole-cell patch-clamp recording in the zebrafish brain , Quantifying aggressive behavior in zebrafish , Correlating whole brain neural activity with behavior in head-fixed larval zebrafish , Practical guide to light sheet microscopy , Calcium imaging of neuronal activity in free-swimming larval zebrafish , Fiber optic-based photostimulation of larval zebrafish , Genetic ablation, sensitization, and isolation of neurons using nitroreductase and tetrodotoxin-insensitive channels

Additional Edition: ISBN 9781493937714

Language: English

Keywords: Zebrabärbling ; Tiermodell ; Molekularbiologie ; Genetik ; Krankheit ; Neurowissenschaften ; Laboratory Manuals

URL: Cover

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Format: 1 DVD, farb., stereo , 12 cm

Series Statement: Three ... Extremes 2

Note: Orig.: Japan 2004 , Enth. außerdem: 2 unselbständige Werke , japan., Untertitel in traditional chinese, simplified chinese, engl.

Language: Japanese

Author information: Miike, Takashi 1960-

UID:

Format: 1 DVD Video (ca. 105 Min.) , Tonformat: DD/Stereo, DD/5.1 , NTSC , 1 Beil. , Bildformat: Letter Box

Series Statement: Platinum classics : [DVD Video] 22

Content: Tsugumi, who lives in a small seaside town, has been in delicate health from birth. Her parents spoil her and she is rough and selfish. During summer, Tsugumi meets and falls in love with Kyoichi, who works in the art gallery of the town. One of Tsugumi's old boyfriends, the leader of a gang of juvenile delinquents, attacks Kyoichi and kills Tsugumi's pet dog out of jealousy. Tsugumi decides to plan revenge... (Covertext)

Note: Ländercode: 0 (Kennzeichnung 3) , Japan. mit chines., engl. Untertiteln

Language: Japanese

Author information: Yoshimoto, Banana

UID:

Format: 1 online resource (XIV, 360 p. 94 illus., 85 illus. in color.)

Edition: 3rd ed. 2024.

ISBN: 1-0716-3401-1

Series Statement: Methods in Molecular Biology, 2707

Content: This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the vertebrate nervous system, tools and approaches using zebrafish to study stem cell and regenerative biology, as well as techniques in genetics and genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Zebrafish: Methods and Protocols, Third Edition serves as an invaluable guide to propel advances in developmental biology, disease modeling, and regeneration research using zebrafish and medaka as model systems.

Note: Delivering Traumatic Brain Injury to Larval Zebrafish -- Functional Genomics of Novel Rhabdomyosarcoma Fusion-Oncogenes Using Zebrafish -- Methods to Study Liver Disease Using Zebrafish Larvae -- Developmental Toxicity Assessment Using Zebrafish-Based High Throughput Screening -- Cancer Modeling by Transgene Electroporation in Adult Zebrafish (TEAZ) -- Lineage Tracing of Bone Cells in the Regenerating Fin and during Repair of Bone Lesions -- Primary Culture of Neuronal Populations for Various Downstream Applications -- Holographic Optogenetic Activation of Neurons Eliciting Locomotion in Head-Embedded Larval Zebrafish -- Brain Imaging and Registration in Larval Zebrafish -- Simultaneous Behavioral and Neuronal Imaging by Tracking Microscopy -- Genetic Identification of Neural Circuits Essential for Active Avoidance Fear Conditioning in Adult Zebrafish -- Quantitative Live Imaging of Zebrafish Scale Regeneration: From Adult Fish to Signaling Patterns and Tissue Flows -- Generation of Conditional Knockout Zebrafish Using an Invertible Gene-Trap Cassette -- Spinal Cord Injury and Assays for Regeneration -- Selective Cell Ablation Using an Improved Prodrug-Converting Nitroreductase -- Section Immunostaining for Protein Expression and Cell Proliferation Studies of Regenerating Fins -- In Vivo Optogenetic Phase Transition of an Intrinsically Disordered Protein -- Colorimetric Barcoding to Track, Isolate, and Analyze Hematopoietic Stem Cell Clones -- Mutation Knock-In Methods Using Single-Stranded DNA and Gene Editing Tools in Zebrafish -- Generation of Transgenic Fish Harboring CRISPR/Cas9-Mediated Somatic Mutations via a tRNA-Based Multiplex sgRNA Expression -- Scalable CRISPR Screens in Zebrafish Using MIC-Drop -- The Goldfish Genome and Its Utility for Understanding Gene Regulation and Vertebrate Body Morphology.

Additional Edition: ISBN 1-0716-3400-3

Language: English

DOI: 10.1007/978-1-0716-3401-1

UID:

Format: 1 online resource (XIV, 360 p. 94 illus., 85 illus. in color.)

Edition: 3rd ed. 2024.

ISBN: 1-0716-3401-1

Series Statement: Methods in Molecular Biology, 2707

Content: This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the vertebrate nervous system, tools and approaches using zebrafish to study stem cell and regenerative biology, as well as techniques in genetics and genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Zebrafish: Methods and Protocols, Third Edition serves as an invaluable guide to propel advances in developmental biology, disease modeling, and regeneration research using zebrafish and medaka as model systems.

Note: Delivering Traumatic Brain Injury to Larval Zebrafish -- Functional Genomics of Novel Rhabdomyosarcoma Fusion-Oncogenes Using Zebrafish -- Methods to Study Liver Disease Using Zebrafish Larvae -- Developmental Toxicity Assessment Using Zebrafish-Based High Throughput Screening -- Cancer Modeling by Transgene Electroporation in Adult Zebrafish (TEAZ) -- Lineage Tracing of Bone Cells in the Regenerating Fin and during Repair of Bone Lesions -- Primary Culture of Neuronal Populations for Various Downstream Applications -- Holographic Optogenetic Activation of Neurons Eliciting Locomotion in Head-Embedded Larval Zebrafish -- Brain Imaging and Registration in Larval Zebrafish -- Simultaneous Behavioral and Neuronal Imaging by Tracking Microscopy -- Genetic Identification of Neural Circuits Essential for Active Avoidance Fear Conditioning in Adult Zebrafish -- Quantitative Live Imaging of Zebrafish Scale Regeneration: From Adult Fish to Signaling Patterns and Tissue Flows -- Generation of Conditional Knockout Zebrafish Using an Invertible Gene-Trap Cassette -- Spinal Cord Injury and Assays for Regeneration -- Selective Cell Ablation Using an Improved Prodrug-Converting Nitroreductase -- Section Immunostaining for Protein Expression and Cell Proliferation Studies of Regenerating Fins -- In Vivo Optogenetic Phase Transition of an Intrinsically Disordered Protein -- Colorimetric Barcoding to Track, Isolate, and Analyze Hematopoietic Stem Cell Clones -- Mutation Knock-In Methods Using Single-Stranded DNA and Gene Editing Tools in Zebrafish -- Generation of Transgenic Fish Harboring CRISPR/Cas9-Mediated Somatic Mutations via a tRNA-Based Multiplex sgRNA Expression -- Scalable CRISPR Screens in Zebrafish Using MIC-Drop -- The Goldfish Genome and Its Utility for Understanding Gene Regulation and Vertebrate Body Morphology.

Additional Edition: ISBN 1-0716-3400-3

Language: English

DOI: 10.1007/978-1-0716-3401-1

UID:

Format: 1 online resource (XIV, 360 p. 94 illus., 85 illus. in color.)

Edition: 3rd ed. 2024.

ISBN: 1-0716-3401-1

Series Statement: Methods in Molecular Biology, 2707

Content: This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the vertebrate nervous system, tools and approaches using zebrafish to study stem cell and regenerative biology, as well as techniques in genetics and genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Zebrafish: Methods and Protocols, Third Edition serves as an invaluable guide to propel advances in developmental biology, disease modeling, and regeneration research using zebrafish and medaka as model systems.

Note: Delivering Traumatic Brain Injury to Larval Zebrafish -- Functional Genomics of Novel Rhabdomyosarcoma Fusion-Oncogenes Using Zebrafish -- Methods to Study Liver Disease Using Zebrafish Larvae -- Developmental Toxicity Assessment Using Zebrafish-Based High Throughput Screening -- Cancer Modeling by Transgene Electroporation in Adult Zebrafish (TEAZ) -- Lineage Tracing of Bone Cells in the Regenerating Fin and during Repair of Bone Lesions -- Primary Culture of Neuronal Populations for Various Downstream Applications -- Holographic Optogenetic Activation of Neurons Eliciting Locomotion in Head-Embedded Larval Zebrafish -- Brain Imaging and Registration in Larval Zebrafish -- Simultaneous Behavioral and Neuronal Imaging by Tracking Microscopy -- Genetic Identification of Neural Circuits Essential for Active Avoidance Fear Conditioning in Adult Zebrafish -- Quantitative Live Imaging of Zebrafish Scale Regeneration: From Adult Fish to Signaling Patterns and Tissue Flows -- Generation of Conditional Knockout Zebrafish Using an Invertible Gene-Trap Cassette -- Spinal Cord Injury and Assays for Regeneration -- Selective Cell Ablation Using an Improved Prodrug-Converting Nitroreductase -- Section Immunostaining for Protein Expression and Cell Proliferation Studies of Regenerating Fins -- In Vivo Optogenetic Phase Transition of an Intrinsically Disordered Protein -- Colorimetric Barcoding to Track, Isolate, and Analyze Hematopoietic Stem Cell Clones -- Mutation Knock-In Methods Using Single-Stranded DNA and Gene Editing Tools in Zebrafish -- Generation of Transgenic Fish Harboring CRISPR/Cas9-Mediated Somatic Mutations via a tRNA-Based Multiplex sgRNA Expression -- Scalable CRISPR Screens in Zebrafish Using MIC-Drop -- The Goldfish Genome and Its Utility for Understanding Gene Regulation and Vertebrate Body Morphology.

Additional Edition: ISBN 1-0716-3400-3

Language: English

DOI: 10.1007/978-1-0716-3401-1