Kooperativer Bibliotheksverbund

UID:

Format: XI, 341 S. , Ill., graph. Darst.

Edition: 2. ed.

ISBN: 9781607616283

Series Statement: Methods in molecular biology 630

Language: English

Subjects: Biology

Keywords: Reverse Transkriptase-Polymerase-Kettenreaktion ; Methode ; Aufsatzsammlung ; Aufsatzsammlung

URL: Inhaltsverzeichnis

URL: Inhaltsverzeichnis

UID:

Format: 1 online resource (XI, 341p. 65 illus., 4 illus. in color.)

Edition: Second Edition.

ISBN: 1-60761-629-7

Series Statement: Methods in Molecular Biology, 630

Content: Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. User friendly and up-to-date, RT-PCR Protocols, Second Edition acts as a handy companion to scientists from numerous diverse backgrounds who wish to explore further the marvels of gene expression.

Note: Bibliographic Level Mode of Issuance: Monograph , The RT-PCR Detective: Hunting Down the Best Method -- Single Cell RT-PCR on Mouse Embryos: A General Approach for Developmental Biology -- Poly(A) cDNA Real-Time PCR for Indicator Gene Measurement in Cancer -- Transcriptome Profiling of Host–Microbe Interactions by Differential Display RT-PCR -- Quantitative RT-PCR Methods for Mature microRNA Expression Analysis -- Detection of Influenza A Virus Neuraminidase and PB2 Gene Segments by One Step Reverse Transcription Polymerase Chain Reaction -- Detection and Identification of CD46 Splicing Isoforms by Nested RT-PCR -- Simultaneous Detection of Bluetongue Virus RNA, Internal Control GAPDH mRNA, and External Control Synthetic RNA by Multiplex Real-Time PCR -- Detection of West Nile Viral RNA from Field-Collected Mosquitoes in Tropical Regions by Conventional and Real-Time RT-PCR -- Detection of Antisense RNA Transcripts by Strand-Specific RT-PCR -- RT-PCR Amplification and Cloning of Large Viral Sequences -- The RT-PCR Mathematician: Assessing Gene and RNA Expression -- One-Step RT-LATE-PCR for mRNA and Viral RNA Detection and Quantification -- Changes in Gene Expression of Caveolin-1 After Inflammatory Pain Using Quantitative Real-Time PCR -- Real-Time Quantitative Reverse Transcriptase Polymerase Chain Reaction -- The Use of Comparative Quantitative RT-PCR to Investigate the Effect of Cysteine Incubation on GPx1 Expression in Freshly Isolated Cardiomyocytes -- The Renaissance of Competitive PCR as an Accurate Tool for Precise Nucleic Acid Quantification -- The RT-PCR Master Chef: Finding the Right Ingredients -- Skeletal Muscle RNA Extraction in Preparation for RT-PCR -- Reverse Transcription of the Ribonucleic Acid: The First Step in RT-PCR Assay -- Primer Design for RT-PCR -- Hot Start PCR -- Real-time RT-PCR for Automated Detection of HIV-1 RNA During Blood Donor Screening. , English

Additional Edition: ISBN 1-60761-628-9

Language: English

DOI: 10.1007/978-1-60761-629-0

UID:

Format: 1 online resource (394 p.)

Edition: 1st ed. 2002.

ISBN: 9786610830848 , 1-59259-283-X , 1-280-83084-0

Series Statement: Methods in Molecular Biology, 193

Content: RT-PCR, the most widely used technique in biomedical research for analyzing gene expression, has proven a significant boon to the molecular investigation of disease pathogenesis. In RT-PCR Protocols, Joe O'Connell has assembled a panel of highly regarded molecular biologists and clinical researchers to describe in detail their most novel, useful, and interesting RT-PCR applications. Here the newcomer will find readily reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For the expert seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering. Each protocol emphasizes the technical steps critical for experimental success and includes tips on avoiding potential pitfalls, notes explaining the method's scientific basis and its possible sources of error, and comments on how it might best be accommodated to various experimental circumstances. Diverse and wide-ranging, RT-PCR Protocols offers investigators powerful techniques to be used in the molecular investigation of disease pathogenesis and shows how RT-PCR can complement other technological advances in the design of today's new therapeutic strategies.

Note: Description based upon print version of record. , RT-PCR in Biomedicine -- The Basics of RT-PCR -- Highly Sensitive Detection and Analysis of mRNA -- Using the Quantitative Competitive RT-PCR Technique to Analyze Minute Amounts of Different mRNAs in Small Tissue Samples -- Detection of mRNA Expression and Alternative Splicing in a Single Cell -- Nested RT-PCR -- Quantitative RT-PCR -- Quantitative RT-PCR -- Rapid Development of a Quantitative-Competitive (qc) RT-PCR Assay Using a Composite Primer Approach -- Quantitation of Gene Expression by RT-PCR and HPLC Analysis of PCR Products -- Time-Resolved Fluorometric Detection of Cytokine mRNAs Amplified by RT-PCR -- Mimic-Based RT-PCR Quantitation of Substance P mRNA in Human Mononuclear Phagocytes and Lymphocytes -- Detection and Analysis of RNA Viruses -- Detection and Quantification of the Hepatitis C Viral Genome -- Semi-Quantitative Detection of Hepatitis C Virus RNA by “Real-Time” RT-PCR -- RT-PCR for the Assessment of Genetically Heterogenous Populations of the Hepatitis C Virus -- In Situ Localization of mRNA Expression -- in situ Immuno-PCR -- RT-PCR from Laser-Capture Microdissected Samples -- Mycobacterium paratuberculosis Detected by Nested PCR in Intestinal Granulomas Isolated by LCM in Cases of Crohn’s Disease -- RT-PCR-Based Approaches to Generate Probes for mRNA Detection by In Situ Hybridization -- Differential mRNA Expression -- Amplified RNA for Gene Array Hybridizations -- Semi-Quantitative Determination of Differential Gene Expression in Primary Tumors and Matched Metastases by RT-PCR -- Genetic Analysis -- Detection of Single Nucleotide Polymorphisms Using a Non-Isotopic RNase Cleavage Assay -- RT-PCR in Immunology -- Detection of Clonally Expanded T-Cells by RT-PCR-SSCP and Nucleotide Sequencing of T-Cell Receptor ?-CDR3 Regions -- Generation of scFv from a Phage Display Mini-Library Derived from Tumor-Infiltrating B-Cells -- Generation of Murine scFv Intrabodies from B-Cell Hybridomas -- Quantitation of mRNA Levels by RT-PCR in Cells Purified by FACS -- RT-PCR in Anti-Sense Technology -- Detection of Anti-Sense RNA Transcripts by Anti-Sense RT-PCR -- RT-PCR in cDNA Cloning -- RT-PCR in cDNA Library Construction -- An RT-PCR-Based Protocol for the Rapid Generation of Large, Representative cDNA Libraries for Expression Screening. , English

Additional Edition: ISBN 0-89603-875-0

Additional Edition: ISBN 0-585-44216-9

Language: English

DOI: 10.1385/159259283X

UID:

Format: X, 197 S.

ISBN: 0-7486-1115-0

Series Statement: Tendencies: identities, texts, cultures

Additional Edition: Erscheint auch als Online-Ausgabe ISBN 978-0-7486-1116-4

Language: English

Subjects: American Studies , English Studies

Keywords: Englisch ; Roman ; Identität ; Englisch ; Roman ; Autobiografie ; Englisch ; Ich-Roman ; Erinnerung ; Identität ; Englisch ; Autobiografischer Roman ; Erinnerung ; Identität ; Autobiografischer Roman ; Erinnerung ; Identität ; Ich-Roman ; Erinnerung ; Identität ; Frauenliteratur ; Autobiografischer Roman

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Format: 1 online resource (208 p.)

ISBN: 9780748611164

Series Statement: Tendencies: Identities, Texts, Cultures : TITC

Content: It is commonly accepted that identity or a sense of self is constructed by and through narrative - the stories we tell ourselves and each other about our lives. This book explores the complex relationships that exist between memory, nostalgia, writing and identity. The author examines a range of autobiographical and first-person fictional texts from holocaust literature, women's writing and popular fiction. Each text foregrounds issues of memory, history and trauma in the construction of identity. There are close readings of texts including Sylvia Fraser's My Father's House, Margaret Atwood's Cats Eye, Barbara Vine's A DarkAdapted Eye, Toni Morrison's Beloved, George Perec's W Or the Memory of Childhood, and Anne Michael's Fugitive Pieces. Reading these texts of memory shows that 'remembering the self' depends not on restoring an original identity, but on 're-membering', on putting past and present selves together, moment by moment, in a process of provisional reconstruction. This is a powerful contribution to the growing field of 'trauma' and holocaust studies. It will be of relevance to those working in the areas of literary and cultural studies, which are witnessing a steady growth of interest in autobiography, theories of narrative, and the relationship between trauma, history and memory.Key FeaturesClose readings of recent fiction and autobiography, including Toni Morrison's Beloved, Anne Michaels' Fugitive Pieces and the popularthrillers of Barbara VineAn interrogation of the models of memory which underpin the constructionof narratives and of identities, and of cultureA focus on meaning as constructed by the readers of the narrative textAn exploration of the myths of 'origin'

Note: Frontmatter -- , Contents -- , Acknowledgements -- , Series Editor’s Introduction -- , Introduction: ‘But we didn’t know that then’ -- , 1. Memory in Theory -- , 2. Present Imperfect Translation: Ronald Fraser’s In Search o f a Past and Carolyn Steedman’s Landscape fo r a Good Woman -- , 3. ‘A life entire’: Narrative Reconstruction in Sylvia Fraser’s My Father's House and Margaret Atwood’s Cat's Eye -- , 4. Myths of Origin: Identity, Memory and Detection in Barbara Vine’s A Dark Adapted Eye and Asta's Book -- , 5. Holocaust, Memory, Representation: Georges Perec’s W or The Memory o f Childhood and Anne Michaels’ Fugitive Pieces -- , 6. Rememory and Reconstruction: Toni Morrison’s Beloved -- , Afterword -- , Bibliography -- , Index , In English.

Additional Edition: ISBN 9780748611157

Language: English

DOI: 10.1515/9780748611164

URL: https://doi.org/10.1515/9780748611164

URL: https://www.degruyter.com/isbn/9780748611164

URL: https://doi.org/10.1515/9780748611164

URL: https://www.degruyter.com/isbn/9780748611164

UID:

Format: X, 197 S.

Edition: [Nachdr.]

ISBN: 0-7486-1115-0

Series Statement: Tendencies: identities, texts, cultures

Language: English

Subjects: American Studies , English Studies

Keywords: Englisch ; Roman ; Identität ; Englisch ; Roman ; Autobiografie ; Englisch ; Ich-Roman ; Erinnerung ; Identität ; Englisch ; Autobiografischer Roman ; Erinnerung ; Identität ; Autobiografischer Roman ; Erinnerung ; Identität ; Ich-Roman ; Erinnerung ; Identität ; Frauenliteratur ; Autobiografischer Roman

UID:

Format: 1 online resource (208 p.)

Edition: 1st ed.

ISBN: 0-7486-1116-9 , 0-585-44174-X

Series Statement: Tendencies Memory, narrative, identity

Content: It is commonly accepted that identity or a sense of self is constructed by and through narrative - the stories we tell ourselves and each other about our lives. This book explores the complex relationships that exist between memory, nostalgia, writing and identity. The author examines a range of autobiographical and first-person fictional texts from holocaust literature, women's writing and popular fiction. Each text foregrounds issues of memory, history and trauma in the construction of identity. There are close readings of texts including Sylvia Fraser's My Father's House, Margaret Atwood's Cats Eye, Barbara Vine's A DarkAdapted Eye, Toni Morrison's Beloved, George Perec's W Or the Memory of Childhood, and Anne Michael's Fugitive Pieces. Reading these texts of memory shows that 'remembering the self' depends not on restoring an original identity, but on 're-membering', on putting past and present selves together, moment by moment, in a process of provisional reconstruction. This is a powerful contribution to the growing field of 'trauma' and holocaust studies. It will be of relevance to those working in the areas of literary and cultural studies, which are witnessing a steady growth of interest in autobiography, theories of narrative, and the relationship between trauma, history and memory.Key FeaturesClose readings of recent fiction and autobiography, including Toni Morrison's Beloved, Anne Michaels' Fugitive Pieces and the popularthrillers of Barbara VineAn interrogation of the models of memory which underpin the constructionof narratives and of identities, and of cultureA focus on meaning as constructed by the readers of the narrative textAn exploration of the myths of 'origin'

Note: Bibliographic Level Mode of Issuance: Monograph , Frontmatter -- , Contents -- , Acknowledgements -- , Series Editor’s Introduction -- , Introduction: ‘But we didn’t know that then’ -- , 1. Memory in Theory -- , 2. Present Imperfect Translation: Ronald Fraser’s In Search o f a Past and Carolyn Steedman’s Landscape fo r a Good Woman -- , 3. ‘A life entire’: Narrative Reconstruction in Sylvia Fraser’s My Father's House and Margaret Atwood’s Cat's Eye -- , 4. Myths of Origin: Identity, Memory and Detection in Barbara Vine’s A Dark Adapted Eye and Asta's Book -- , 5. Holocaust, Memory, Representation: Georges Perec’s W or The Memory o f Childhood and Anne Michaels’ Fugitive Pieces -- , 6. Rememory and Reconstruction: Toni Morrison’s Beloved -- , Afterword -- , Bibliography -- , Index , English

Additional Edition: ISBN 0-7486-1115-0

Language: English

DOI: 10.1515/9780748611164

UID:

Format: 7 , graph. Darst.

ISSN: 1471-2954

Content: The oceanic abyss (depths greater than 3000m), one of the largest environments on the planet, is characterized by absence of solar light, high pressures and remoteness from surface food supply necessitating special molecular, physiological, behavioural and ecological adaptations of organisms that live there. Sampling by trawl, baited hooks and cameras we show that the Chondrichthyes (sharks, rays and chimaeras) are absent from, or very rare in this region. Analysis of a global data set shows a trend of rapid disappearance of chondrichthyan species with depth when compared with bony fishes. Sharks, apparently well adapted to life at high pressures are conspicuous on slopes down to 2000m including scavenging at food falls such as dead whales. We propose that they are excluded from the abyss by high-energy demand, including an oil-rich liver for buoyancy, which cannot be sustained in extreme oligotrophic conditions. Sharks are apparently confined to ca 30% of the total ocean and distribution of many species is fragmented around sea mounts, ocean ridges and ocean margins. All populations are therefore within reach of human fisheries, and there is no hidden reserve of chondrichthyan biomass or biodiversity in the deep sea. Sharks may be more vulnerable to over-exploitation than previously thought.

In: Royal Society (London), Proceedings of the Royal Society of London. Series B, Biological sciences, London : The Royal Society, 1905, (2006), 1471-2954

In: year:2006

In: extent:7

Language: English

DOI: 10.1098/rspb.2005.3461