UID:
almafu_9958080607402883
Format:
1 online resource (417 p.)
ISBN:
1-281-05685-5
,
9786611056858
,
0-08-053646-8
Series Statement:
Methods in Neurosciences ; Volume 27
Content:
This practical book provides the detailed methodology and expert guidance required for measuring and manipulating cytosolic ion concentrations. In addition, the strengths, weaknesses, and pitfalls of various techniques are presented. It is an invaluable source for those needing an objective evaluation of current methodologies and for those contemplating setting up such procedures.Key Features* A one-source reference for measuring and manipulating intracellular ions and for comparing and evaluating current methodologies* Includes overviews of* Optical probes and reagents
Note:
Description based upon print version of record.
,
Front Cover; Measurement and Manipulation of Intracellular Ions; Copyright Page; Table of Contents; Contributors to Volume 27; Preface; Volumes in Series; Chapter 1. Light-Optical-Based Reagents for the Measurement and Manipulation of Ions, Metabolites, and Macromolecules in Living Cells; Chapter 2. Use of Ion-Selective Microelectrodes for Recording Intracellular Ion Levels; Chapter 3. Use of NMR Spectroscopy to Measure Intracellular Ion Concentrations; Chapter 4. Use of Ionophores for Manipulating Intracellular Ion Concentrations
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Chapter 5. Fluorescence Imaging of Cytosolic Calcium: An Introduction to Basic Experimental PrinciplesChapter 6. Fluorescence Measurements of Cytosolic Calcium: Combined Photometry with Electrophysiology; Chapter 7. Use of Chelators and Photoactivatable Caged Compounds to Manipulate Cytosolic Calcium; Chapter 8. Measurement of Cytosolic Calcium by 19F NMR; Chapter 9. Fluorescence Measurement of Cytosolic pH in Cultured Rodent Astrocytes; Chapter 10. Brain Cell Acid-Base Changes Measured with Ion-Selective Microelectrodes
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Chapter 11. Measurement of Cytosolic pH by Nuclear Magnetic Resonance SpectroscopyChapter 12. Manipulation and Regulation of Cytosolic pH; Chapter 13. Fluorescence Measurements of Cytosolic Sodium Concentration; Chapter 14. Measurement of Cytosolic Sodium Using Ion-Selective Microelectrodes; Chapter 15. A Practical Guide to the Use of Fluorescent Indicators for the Measurement of Cytosolic Free Magnesium; Chapter 16. Measurement of Cytosolic Magnesium by 31P NMR Spectroscopy; Chapter 17. Chloride-Sensitive Fluorescent Indicators
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Chapter 18. Measurement of Cytosolic Chloride Activity by Ion-Selective MicroelectrodesChapter 19. Use of Ion-Selective Microelectrodes and Fluorescent Probes to Measure Cell Volume; Index
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English
Additional Edition:
ISBN 0-12-185297-0
Language:
English
Keywords:
Electronic books.
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