Format:
1 Online-Ressource (ix, 159 Seiten, 6003 KB)
,
Illustrationen, Diagramme
Content:
Microalgae have been recognized as a promising green production platform for recombinant proteins. The majority of studies on recombinant protein expression have been conducted in the green microalga C. reinhardtii. While promising improvement regarding nuclear transgene expression in this alga has been made, it is still inefficient due to epigenetic silencing, often resulting in low yields that are not competitive with other expressor organisms. Other microalgal species might be better suited for high-level protein expression, but are limited in their availability of molecular tools. The red microalga Porphyridium purpureum recently emerged as candidate for the production of recombinant proteins. It is promising in that transformation vectors are episomally maintained as autonomously replicating plasmids in the nucleus at a high copy number, thus leading to high expression values in this red alga. In this work, we expand the genetic tools for P. purpureum and investigate parameters that govern efficient transgene expression. We ...
Note:
Dissertation Universität Potsdam 2024
Additional Edition:
Erscheint auch als Druck-Ausgabe Hammel, Alexander Establishing the red microalga Porphyridium purpureum as a novel platform for the production of recombinant proteins Potsdam, 2024
Language:
English
Keywords:
Hochschulschrift
DOI:
10.25932/publishup-63270
URN:
urn:nbn:de:kobv:517-opus4-632709
Bookmarklink