Format:
Online-Ressource (XXXI, 505 S.)
Edition:
Online-Ausg.
ISBN:
9780121821838
Series Statement:
Methods in enzymology 282
Content:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features * This volume and its companion Volumes 279, 280, and 281 provide * A collation of the most recent and useful methods for the identification, preparation, and quantification of vitamins and coenzymes * Details on physical, chemical, and biological properties of vitamins and coenzymes * Chemical and biological syntheses of vitamins, coenzymes, and their analogs * Aspects of transport and metabolism of vitamins and coenzymes
Note:
Includes bibliographical references and indexes
,
Atmospheric pressure chemical ionization and electron capture negative chemical ionization mass spectrometry in studying [beta]-Carotene conversion to retinol in humans -- Synthesis of Vitamin Db3s and 1[alpha],25-Dihydroxy Vitamin Db3s -- Assay of 1,25-dihydroxyvitamin Db3s from serum samples -- Quantitation of 25-Hydroxyvitamin D and 1,25-Dihydroxyvitamin D -- Assay of vitamin D derivatives and purification of vitamin D hydroxylases -- Assay of 25-Hydroxyvitamin D 1[alpha]-Hydroxlase and 24-Hydroxylase -- Molecular cloning of vitamin Db3s hydroxylases -- Role of 1[alpha],25-dihydroxyvitamin Db3s in osteoclast differentiation and function -- Assay of direct effect of 1,25-Dihydroxyvitamin Db3s on calcium ion -- Vitamin E status and immune function -- Inhibition of platelet adhesion as functional test for vitamin E status -- Inhibition of plasma cholesterol ester hydroperoxide -- [alpha]-Tocopherol-binding proteins -- [alpha]-Carboxyethyl 6-Hydrozychroman.
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Generation and characterization of cellular retinoic acid-binding proteins from Escherichia coli expression systems -- Generation and characterizing retinoid receptors from Escherichia coli and insect cell expression systems -- Expression and characterization of retinoid receptors in yeast -- Use of in situ hybridization techniques to study embryonic expression of retinoid receptors and binding proteins -- Use of quantitative polymerase chain reaction to study retinoid receptor expression -- Use of quantitative polymerase chain reaction to study cellular retinoic acid-binding protein-II mRNA expression -- Use of transgenic mice to study activation of retinoic acid-responsive promoters -- Use of transgenic mice to eliminate retinoic acid receptor function -- Use of reporter cells to study endogenous retinoid sources -- Preparation of radiolabeled 9-cis- and all-trans-retinoids -- Identification and quantification of retinoic acid and other metabolites from [beta]-Carotene excentric cleavage -- Assessing metabolism of [beta]-Carotene.
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Purification of vitamin K-dependent carboxylase from cultured cells -- Purification of native bovine carboxylase -- Purification of [gamma]-Glutamyl carboxylase -- Assay of vitamin K-dependent carboxylase activity -- Expression of human anticoagulation protein C and [gamma]-Carboxyglutamic acid mutants -- Determination of site-specific [gamma]-carboxyglutamin acid formation -- Purification of warfarin-sensitive vitamin K epoxide reductase -- Determination of vitamin K compounds in plasma or serum -- Assay of phylloquinone in plasma -- Assay of menaquinones in plasma -- Assay of phylloquinone and menaquinones in human liver -- Determination of phylloquinone in foods -- Assay of menaquinones in bacterial cultures, stool samples, and intestinal contents.
In:
Pt. L
Additional Edition:
Erscheint auch als Druck-Ausgabe Vitamins and coenzymes ; Pt. L San Diego, Calif. [u.a.] : Acad. Press, 1997 ISBN 0121821838
Language:
English
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