UID:
almahu_9949251376102882
Format:
1 online resource (XIV, 396 p. 82 illus., 48 illus. in color.)
Edition:
1st ed. 2015.
ISBN:
1-4939-2152-5
Series Statement:
Methods in Molecular Biology, 1254
Content:
This volume represents a valuable and readily reproducible collection of established and emerging techniques for neuronal cell death research. Conveniently divided into four parts, sections cover a series of techniques for the molecular, structural, functional and genomic characterization of dying neurons, a number of protocols that are of primary interest in neuropathology and in experimental neuropathology, a series of gene engineering techniques to obtain and manipulate neuronal stem cells and progenitors, to prepare HSV-1 vectors for the gene therapy, and to CNS transplantation of bone marrow stem cells, and finally, some very interesting protocols for the study of cell death in non-mammalian models. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Neuronal Cell Death: Methods and Protocols seeks to serve a large audience of scientists that are currently active in the field or are willing to enter such an exciting and still expanding area of neurobiology.
Note:
Bibliographic Level Mode of Issuance: Monograph
,
Neuronal Cell Death: An Overview of its Different Forms in Central and Peripheral Neurons -- Cell Volume Regulation Monitored with Combined Epifluorescence and Digital Holographic Microscopy -- Flow Cytometric Analysis of DNA Synthesis and Apoptosis in Central Nervous System Using Fresh Cell Nuclei -- Nuclear Signs of Pre-Neurodegeneration -- Multi-Parametric O2 Imaging in Three-Dimensional Neural Cell Models with the Phosphorescent Probes -- Calcium Imaging in Neuron Cell Death -- Monitoring Mitochondrial Membranes Permeability in Live Neurons and Mitochondrial Swelling through Electron Microscopy Analysis -- Real-Time Visualization of Caspase-3 Activation by Fluorescence Resonance Energy Transfer (FRET) -- Design and Cloning of Short Hairpin RNAs (shRNAs) into a Lentiviral Silencing Vector to -- Genomic Analysis Using Affymetrix Standard Microarray GeneChips (169 Format) in Degenerate Murine Retina -- Genomic Analysis of Transcriptional Changes Underlying Neuronal Apoptosis -- High-Throughput Cell Death Assays -- Image Analysis Algorithms for Immunohistochemical Assessment of Cell Death -- In Vitro Oxygen-Glucose Deprivation to Study Ischemic Cell Death -- Laser Microbeam Targeting of Single Nerve Axons in Cell Culture -- Real-Time Imaging of Retinal Cell Apoptosis by Confocal Scanning Laser Ophthalmoscopy -- Targeted Toxicants to Dopaminergic Neuronal Cell Death -- Stem Cells, Neural Progenitors, and Engineered Stem Cells -- Herpes Simplex Virus Type 1 (HSV-1)-Derived Recombinant Vectors for Gene Transfer and Gene Therapy -- Herpes Simplex Virus Type 1 (HSV-1)-Derived Amplicon Vectors for Gene Transfer and Gene Therapy -- Bone Marrow Transplantation for Research and Regenerative Therapies in the Central Nervous System -- Detection of Activated Caspase-8 in Injured Spinal Axons by Using Fluorochrome-Labeled Inhibitors of Caspases (FLICA) -- Generation of Zebrafish Models by CRISPR/Cas9 Genome Editing -- In Vivo Assessment of Neuronal Cell Death in Drosophila -- Drosophila Model for Studying Phagocytosis Following Neuronal Cell Death.
,
English
Additional Edition:
ISBN 1-4939-2151-7
Language:
English
Keywords:
Laboratory Manuals.
;
Laboratory Manuals.
;
Laboratory Manuals.
;
Laboratory Manuals.
DOI:
10.1007/978-1-4939-2152-2
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