Format:
1 Online-Ressource (xii, 343 Seiten)
,
Illustrationen, Diagramme
ISBN:
9781788016322
Series Statement:
Food chemistry, function and analysis 15
Content:
Cover; Rapid Antibody-based Technologies in Food Analysis; Contents; Chapter 1 -- Introduction to Antibody-based Food Analysis; Acknowledgments; References; Chapter 2 -- Production and Use of Antibodies; 2.1 Introduction; 2.2 Antibodies: An Introduction; 2.3 Antibody Production; 2.4 Advantages and Limitations of Different Types of Antibodies; 2.5 Selection of a Host for Antibody Production; 2.6 Antibody Selection and Screening; 2.6.1 Limiting Dilution Method; 2.6.2 In Vitro Antibody Display Technologies; 2.6.3 Fluorescence-activated Cell Sorting; 2.6.4 Other Antibody Screening Techniques
Content:
2.7 Antibody Characterization and Validation2.8 Examples of Antibody Applications in the Food Industry; 2.9 Conclusion; References; Chapter 3 -- The Importance of Bioanalytical Parameters and Statistical Analysis in Immunoassays for Food Analytes; 3.1 Introduction; 3.2 Bioanalytical Parameters of an Immunoassay; 3.2.1 Precision and Accuracy; 3.2.2 Sensitivity and Specificity; 3.2.3 Calibration Curve; 3.2.4 Stability; 3.2.5 Recovery; 3.2.6 Bioanalytical Performance; 3.2.6.1 Limit of Blank (LOB), Limit of Detection (LOD), and Limit of Quantification (LOQ)
Content:
3.2.6.2 Lower Limit of the Linear Interval (LLLI) and Lower Limit of the Measuring Interval (LLMI)3.2.6.3 Linear Range and Analytical Measurement Range; 3.2.6.4 Carryover and Errors; 3.2.6.5 Interference and Cross-reactivity; 3.2.6.6 Bias and Method Comparison; 3.2.6.7 Hook Effect and Quality Controls (QCs); 3.3 Critiques and Outlook; 3.4 Conclusions; References; Chapter 4 -- Designing Appropriate Immunoassays for Food Analysis; 4.1 Introduction; 4.2 Detection Methods; 4.3 Appropriate Antibody Selection; 4.4 Immunoassay Design; 4.5 Enhancing Antibody Characteristics for Assay Applications
Content:
4.6 Broad-specificity Immunoassays4.7 Multiplex Assays; 4.8 Functionalisation; 4.9 Assay Variables; 4.10 Sample Preparation and Enrichment; 4.11 Future Perspectives and Rapid Assays; 4.12 Conclusions; References; Chapter 5 -- Targeting Marine Toxins and Other Adulterants in Fish; 5.1 Introduction; 5.2 Antibody-based Methods Commonly Used in the Detection of Marine Contaminants; 5.3 Marine Biotoxins: Lipophilic, Hydrophilic, and Emerging Toxins; 5.3.1 Detection Regulations; 5.3.2 Lipophilic Marine Toxins; 5.3.3 Hydrophilic Toxins; 5.3.4 Screening and Detection Methods
Content:
5.3.4.1 Immunoassays as Screening Methods5.3.4.2 Lateral Flow Immunoassays; 5.3.4.3 Portable and Autonomous Detection Devices; 5.3.5 Emerging Toxins and Their Detection Using Antibody-based Systems; 5.3.6 Bacterially Derived Toxin; 5.4 Other Critical Adulterants Affecting Marine Fish; 5.4.1 Viruses That Mainly Affect Fish; 5.4.1.1 Nodavirus; 5.4.1.2 Infectious Salmon Anemia Virus (ISAV); 5.4.1.3 Infectious Pancreatic Necrosis Virus (IPNV); 5.4.1.4 Shrimp-associated Viruses; 5.4.2 Zoonotic Viruses in Fish That Can Infect Humans; 5.4.2.1 Norovirus
Content:
This book provides a description of antibody-based technologies used in food analysis. It focuses on key applications outlining the approaches used, their advantages and limitations, and future areas for development
Additional Edition:
ISBN 9781788017916
Additional Edition:
ISBN 9781788013901
Additional Edition:
Erscheint auch als Druck-Ausgabe ISBN 9781788013901
Language:
English
DOI:
10.1039/9781788016322
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