Kooperativer Bibliotheksverbund

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Format: 1 online resource (XII, 434 p. 73 illus., 17 illus. in color.)

Edition: 1st ed. 2016.

ISBN: 1-4939-3372-8

Series Statement: Methods in Molecular Biology, 1400

Content: This volume covers the latest protocols designed to identify and characterize TEs in genomes, ancient or recently inserted. Additionally, this book includes a series of protocols designed to understand how host genomes act to regulate the activity of TEs, from elegant genetic mobilization assays to key biochemical methods. Finally, this book also includes chapters that describe how TEs can be used for biotechnological applications. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Transposons and Retrotransposons: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Note: Bibliographic Level Mode of Issuance: Monograph , Study of Transposable Elements and their Genomic Impact -- Bacterial Group II Introns: Identification and Mobility Assay -- In silico Methods to Identify Exapted Transposable Element Families -- Retrotransposon Capture Sequencing (RC-seq): A Targeted, High-Throughput Approach to Resolve Somatic L1 Retrotransposition in Humans -- Long Interspersed Element Sequencing (L1-Seq): A Method to Identify Somatic LINE-1 Insertions in the Human Genome -- Combining Amplification Typing of L1 Active Subfamilies (ATLAS) with High Throughput Sequencing -- RNA-seq Analysis to Measure the Expression of SINE Retroelements -- Qualitative and Quantitative Assays of Transposition and Homologous Recombination of the Retrotransposon Tf1 in Schizosaccharomyces pombe -- LINE Retrotransposition Assays in Saccharomyces cerevisiae -- LINE-1 Cultured Cell Retrotransposition Assay -- L1 Retrotransposition in Neural Progenitor Cells -- Characterization of Engineered L1 Retrotransposition Events: The Recovery Method -- SINE Retrotransposition: Evaluation of Alu Activity and Recovery of de novo Inserts -- The Engineered SVA Trans-Mobilization Assay -- Detection of LINE-1 RNAs by Northern Blot -- Monitoring Long Interspersed Nuclear Element 1 Expression during Mouse Embryonic Stem Cells Differentiation -- Immuno-Detection of Human LINE-1 Expression in Cultured Cells and Human Tissues -- Cellular Localization of Engineered Human LINE-1 RNA and Proteins -- Purification of L1-ribonucleoprotein Particles (L1-RNPs) from Cultured Human Cells -- Characterization of L1-RiboNucleoprotein Particles -- LEAP: L1 Element Amplification Protocol -- Biochemical Approaches to Study LINE-1 Reverse Transcriptase Activity In Vitro -- Methylated DNA Immunoprecipitation Analysis of Mammalian Endogenous Retroviruses -- Profiling DNA Methylation and Hydroxymethylation at Retrotransposable Elements -- A Large-Scale Functional Screen to Identify Epigenetic Repressors of Retrotransposon Expression -- Reprogramming of Human Fibroblasts to Induced Pluripotent Stem Cells with Sleeping Beauty Transposon-based Stable Gene Delivery. . , English

Additional Edition: ISBN 1-4939-3370-1

Language: English

Keywords: Laboratory Manuals ; Laboratory Manuals ; Laboratory Manuals ; Laboratory Manuals

DOI: 10.1007/978-1-4939-3372-3

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Format: X, 184 Bl.

Edition: 1. Aufl.

ISBN: 389649113X

Series Statement: Konstanzer Schriften zur Rechtswissenschaft 111

Note: Zugl.: Konstanz, Univ., Diss., 1996 , Literaturverz. S. 170 - 184

Language: German

Keywords: Mexiko Ley de Inversión extranjera ; USA ; Freihandelsabkommen ; Kanada ; Mexiko ; Hochschulschrift

URL: Inhaltsverzeichnis

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Format: 1 DVD-Video (120 Min., PAL) , color, Regionalcode 2, DVD 9, Bildformat 16:9, Dolby Digital 5.1 , 12 cm

Note: Orig.: E 2007 , Contenidos extra: Trailer, Filmografias, Making Of , Sprache: spanisch - Untertitel: englisch

Language: Spanish

Keywords: Film ; DVD-Video

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Format: 1 Online-Ressource (476 p.)

ISBN: 9788447538010

Series Statement: Col·lecció Filologia UB

Content: The underlying theme of Three Centuries of Greek Culture under the Roman Empire is the idea that, under Roman rule, Greek culture was still alive and dynamic and continued to exert a degree of cultural domination, either real or apparent. So, we hope to analyse the meanings of concepts such as “Greek” or “Greece” in the Empire. Are we right to assume that there was a clear opposition between Greek and Roman? Or would it be more accurate to speak of a “Graeco-Roman world”? It would certainly be possible to make a list of “elements of identity”, on both sides —Greek and Roman—, but, in this case, where should the borders between identity and community be placed? Three Centuries of Greek Culture under the Roman Empire presents several approaches to the period between the second and fourth centuries AD from a variety of angles, perspectives and disciplines. Until now, this time has usually been considered to be the junction of the decline between the classical world and the emergence of the medieval world; however, this book establishes a basis for considering the Imperial period as a specific stage in cultural, historical and social development with a distinct personality of its own

Note: English

Language: English

URL: kostenfrei

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Format: X, 306 S.

ISBN: 978-84-475-3463-0

Note: Zsfassungen in engl. und span. Sprache

Language: Spanish

Subjects: Ancient Studies

Keywords: Latein ; Versdichtung ; Konferenzschrift ; Konferenzschrift ; Aufsatzsammlung

URL: http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024462033&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA

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Format: 1 online resource (259 pages)

Edition: 1st ed.

ISBN: 1-5231-3274-4 , 1-63081-581-0

Series Statement: Technology management and professional development library

Note: Intro -- Practical Model-BasedSystems Engineering -- Contents -- Preface -- Acknowledgments -- CHAPTER 1 Introduction -- 1.1 Book Goals and Readers -- 1.2 Book Content -- 1.3 Diverse Paths for Reading the Book -- References -- CHAPTER 2 Systems Engineering -- 2.1 Definition and Properties of a System -- 2.2 The System Life Cycle -- 2.3 Systems Engineering: A Discipline to Deal with Complexity -- 2.3.1 The Need for Systems Engineering -- 2.3.2 Key Tasks of Systems Engineering -- 2.4 System Development Alternatives -- 2.4.1 Sequential Approach -- 2.4.2 Incremental Approach -- 2.4.3 Evolutionary Approach -- 2.5 Summary -- 2.6 Questions and Exercises -- References -- CHAPTER 3 Model-Based Systems Engineering -- 3.1 Why Is Model-Based Engineering Necessary? -- 3.2 What Is Modeling in MBSE? -- 3.2.1 Why Are Models Used? -- 3.2.1 Models and Views -- 3.3 Modeling Languages, Methods, and Tools for MBSE -- 3.3.1 Modeling Languages -- 3.3.2 MBSE Methodologies -- 3.3.3 MBSE Tools -- 3.4 Summary -- 3.5 Questions and Exercises -- References -- CHAPTER 4 The ISE& -- PPOOA Process -- 4.1 Integrating Systems Engineering and Software Architecting -- 4.2 Challenges of the ISE& -- PPOOA Process -- 4.2.1 Implementing Nonfunctional Requirements -- 4.2.2 Dealing with Functional and Physical Interfaces -- 4.3 Conceptual Model for Systems Engineering with ISE& -- PPOOA -- 4.4 Dimensions and Main Steps of the ISE& -- PPOOA Process -- 4.4.1 The Systems Engineering Subprocess -- 4.4.2 The Software Architecting Subprocess -- 4.5 An Extension of the Process for Energy Efficiency Concerns -- 4.6 Summary -- 4.7 Questions and Exercises -- References -- CHAPTER 5 Functional Architecture -- 5.1 The Importance of Functional Architecture -- 5.1.1 Functional Architecture in Systems Engineering -- 5.1.2 What About Functional Architecture for Software Intensive Systems?. , 5.2 A Function is a Transformation -- 5.2.1 Main Concepts Related to the Functional Architectural Model -- 5.2.2 Functional Architecture Models -- 5.3 Modeling the Functional Hierarchy -- 5.3.1 Top-Down Approach for a Functional Hierarchy -- 5.3.2 Bottom-Up Approach for a Functional Hierarchy -- 5.4 Modeling the Functional Flows -- 5.5 Describing Functions and Functional Interfaces -- 5.6 Functional Requirements -- 5.7 Summary -- 5.8 Questions and Exercises -- References -- CHAPTER 6 Heuristics to Apply in the Engineering of Systems -- 6.1 Heuristics Framework -- 6.2 Systems Architecting Heuristics -- 6.2.1 Heuristics for Step 3: Functional Architecture of the ISE Process -- 6.2.2 Heuristics for Step 4: Physical Architecture of the ISE Process -- 6.3 Reliability and Maintainability Heuristics -- 6.3.1 Reliability and Maintainability Heuristics for Step 4: Physical Architecture of the ISE Process -- 6.3.2 Maintainability Heuristics for Software Architecting: PPOOA Process -- 6.3.3 Heuristics for Restricting the Visibility of Responsibilities -- 6.3.4 Heuristics for Preventing Unintended Effects -- 6.4 Efficiency Heuristics -- 6.4.1 Heuristics for Managing Demand -- 6.4.2 Heuristics for Arbitrating Demand -- 6.4.3 Heuristics for Managing Multiple Resources -- 6.5 Safety Heuristics -- 6.5.1 Heuristics for Hazard Avoidance -- 6.5.2 Heuristics for Hazard Reduction -- 6.5.3 Heuristics for Hazard Control -- 6.5.4 Heuristics for Mitigation of the Effects -- 6.6 Resilience Heuristics -- 6.6.1 Heuristics for Surviving a Threat -- 6.6.2 Heuristics for Adapting to a Threat -- 6.6.3 Heuristics to Degrade Gracefully -- 6.6.4 Heuristics to Act as a Whole in the Face of a Threat -- 6.7 Software Architecting Heuristics Using the PPOOA Framework -- 6.8 Summary -- 6.9 Questions and Exercises -- References -- CHAPTER 7 Physical Architecture. , 7.1 Physical Architecture in Systems Engineering -- 7.1.1 Main Concepts Related to the Physical Architectural Model -- 7.1.2 Functional, Physical, and Quality Trees in ISE& -- PPOOA -- 7.1.3 Other Architecture Models -- 7.2 Allocation and Modularity -- 7.2.1 Representation of the Modular Architecture -- 7.2.2 Allocation -- 7.3 Design Heuristics for Refining the Architecture -- 7.3.1 Control Monitor Pattern -- 7.3.2 Triple Modular Redundancy Pattern -- 7.4 Software Architecting with the PPOOA Framework -- 7.4.1 Domain Model -- 7.4.2 Software Components and the PPOOA Vocabulary -- 7.4.3 Coordination Mechanisms -- 7.4.4 Software Behavior and Causal Flow of Activities -- 7.5 Summary -- 7.6 Questions and Exercises -- References -- CHAPTER 8 Example of Application: Unmanned Aerial Vehicle-Electrical Subsystem -- 8.1 Example Overview, Needs, and Capabilities -- 8.1.1 Operational Scenarios and Use Cases -- 8.1.2 System Capabilities -- 8.2 Functional Architecture and System Requirements -- 8.2.1 Functional Architecture -- 8.2.2 System Requirements -- 8.3 Physical Architecture and Heuristics Applied -- 8.3.1 Heuristics Applied -- 8.3.2 Physical Architecture -- 8.4 Summary -- References -- CHAPTER 9 Example of Application: Collaborative Robot -- 9.1 Example Overview, Needs, and Capabilities -- 9.1.1 Step 1: Identify Operational Scenarios -- 9.1.2 Step 2.a: Capabilities and High-Level Functional Requirements -- 9.1.3 Step 2.b: Quality Attributes and System NFRs -- 9.2 Functional Architecture and System Requirements -- 9.2.1 Functional Architecture -- 9.2.2 System Requirements -- 9.3 Physical Architecture and Heuristics Applied -- 9.3.1 Modular Architecture -- 9.3.2 Application of Heuristics to Refine the Physical Architecture -- 9.3.3 Representation of the Refined Physical Architecture -- 9.4 Software Architecture -- 9.4.1 Software Components. , 9.4.2 Casual Flows of Activities -- 9.4.3 Safety Heuristics -- 9.5 Summary -- References -- CHAPTER 10 Examples of Application: Energy Efficiency for the Steam Generation Process of a Coal Power Plant -- 10.1 Example Overview -- 10.2 Functional Architecture of the Steam Generation Process -- 10.3 Physical Architecture of the Steam Generation Subsystem -- 10.4 Equations and Correlations of the Matter and Energy Balances -- 10.5 Results -- 10.6 Summary -- 10.7 Questions and Exercises -- References -- CHAPTER 11 Trade-Off Analysis -- 11.1 Trade-Off and the Architecture Decision Process -- 11.2 Trade-Off Assessment Criteria and Utility Functions -- 11.3 A Trade-Off Subprocess to be Used with the ISE& -- PPOOA Process -- 11.4 Summary -- References -- CHAPTER 12 Other Topics of Interest and Next Steps -- 12.1 Agile Development -- 12.1.1 Principles and Misconceptions about Agility -- 12.1.2 Scalability in Agile Approaches -- 12.1.3 ISE& -- PPOOA Process and Agility -- 12.2 Architecture Evaluation and Model Checking -- 12.2.1 Architecture Evaluation -- 12.2.2 Diverse Practices for Architecture Evaluation -- 12.2.3 Model Assessment -- 12.3 Next Steps Recommended to the Reader -- 12.4 Summary -- References -- Appendix A SysML Notation -- A.1 Use of SysML in the ISE& -- PPOOA Methodology -- A.1.1 SysML Diagrams in ISE& -- PPOOA -- A.2 SysML for the ISE& -- PPOOA Structural Perspective -- A.2.1 Blocks and Block Definition Diagram -- A.2.2 SysML Internal Block Diagram: Parts, Ports, Connectors, and Flows -- A.3 SysML for the ISE& -- PPOOA Behavioral Perspective -- A.3.1 Activity Nodes -- A.3.2 Control Nodes -- A.4 Other SysML Elements and Views in ISE& -- PPOOA -- A.4.1 Allocation -- A.4.2 Use Case Diagram -- A.4.3 Constraint Blocks and Parametric Diagrams -- A.4.4 Requirements -- A.5 Complementing SysML: PPOOA Architecture Diagram. , References -- Appendix B Requirements Framework -- B.1 Needs, Capabilities, and Requirements -- B.2 Requirements Classification -- B.3 Requirements Flowdown in Systems Development -- B.4 Models and Requirements -- B.5 Requirements Templates -- B.6 Summary -- References -- About the Authors -- Index. , Also available in print.

Additional Edition: ISBN 1-63081-579-9

Language: English

Keywords: Electronic books.

URL: Click to View

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Format: 254 S , zahlr. Ill

ISBN: 848504178X

Language: Spanish

UID:

Format: 1 Online-Ressource (72 S.)

Edition: [Electronic ed.

ISBN: 9788492424368

Series Statement: Documento de trabajo / Laboratorio de Alternativas 2008,137

Note: Literaturverz. S. 63 - 68. - Electronic ed.: [Madrid], 2008. - Speicherung: 18.12.2012. - Adresse: http://www.falternativas.org/en/content/download/13307/410534/version/6/file/Doc137.pdf

Language: Spanish

URL: http://www.falternativas.org/en/content/download/13307/410534/version/6/file/Doc137.pdf

URL: Volltext

Author information: Monereo Pérez, José Luis

UID:

Format: 225 S.

ISBN: 9788498368901

Series Statement: Colección critica del derecho / Derecho vivo : Derecho vivo 43

Language: Spanish

Author information: Monereo Pérez, José Luis

UID:

Format: 253 S

ISBN: 8481643254

Series Statement: Colección Estructuras y procesos

Language: Spanish

Keywords: Spanien ; Sozialrecht ; Arbeitsrecht

URL: Inhaltsverzeichnis

Author information: Monereo Pérez, José Luis