UID:
almafu_9961427342102883
Format:
1 videorecording (46 min., 10 sec.) :
,
sound, color
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004610
Note:
Retrieved April 13, 2024, from https://hstalks.com/bs/2426/.
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Introduction -- Glycosyltransferases (1) -- Donors used by glycosyltransferases (1) -- Donors used by glycosyltransferases (2) -- Nucleotide donors -- Lipid donors for glycosyltransferases -- Acceptors used by glycosyltransferases -- Glycosyltransferase reactions -- Typical mammalian cell surface oligosaccharides -- Diversity of sugars (1) -- Diversity of sugars (2) -- Diversity of sugars (3) -- Blood group A and B structures -- Glycosyltransferases (2) -- Structural folds for nucleotide glycosyltransferases -- Model enzymes for glycosyltransferases -- History of blood group ABO (1) -- History of blood group ABO (2) -- History of blood group ABO (3) -- History of blood group ABO (4) -- History of blood group ABO (5) -- Glycosyltransferases GTA/GTB -- Blood group biochemistry and genetics -- Transmembrane structure and cloning -- Expression and crystallography trials -- GTA and GTB crystals -- GTA and GTB structure -- Findings from 2002 -- 3-deoxy-gal inhibitor -- Ordering of previously disordered structures -- Enzyme structure at substrate binding -- Inhibitors: interference with loop closure -- Electron density for intact donor at active site -- Excellent agreement with NMR conformation -- Comparison with other glycosyltransferases -- Recognition of UDP-gal by GTB: deoxy mapping -- Conclusions so far -- Mechanistic insights (1) -- What is the basis for donor specificity -- Only 2 of 4 critical residues in binding site (1) -- Only 2 of 4 critical residues in binding site (2) -- Which amino acid determines donor specificity? -- Mechanistic insights (2) -- Natural mutants identified in blood banks -- 11 of 200 mutations in blood bank screens -- P156L mutants: GTB no effect on kinetics -- P156L mutants: GTA activity reduced by half -- R176G BAAA: "super A" 4 X increase -- Single point mutation P234S -- M214R GTB near DXD metal binding motif -- Ten new mutations/new hot spots? -- Insights from natural mutants -- Acknowledgements -- Collaborations.
Language:
English
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