UID:
almahu_9949251246902882
Format:
1 online resource (277 p.)
Edition:
1st ed. 2001.
ISBN:
1-61737-204-8
,
9786610830374
,
1-280-83037-9
,
1-59259-234-1
Series Statement:
Methods in Molecular Biology, 170
Content:
Microarray technology, which permits the rapid, simultaneous, and highly sensitive analysis of large numbers of biological samples, is now coming into widespread use for advanced research on gene expression, mutation analysis, proteomics, and gene sequencing. In DNA Arrays: Methods and Protocols, Jang Rampal and a authoritative panel of researchers, engineers, and technologists explain in detail how to design and construct these DNA arrays, as well as how to hybridize them with biological samples for analysis. In step-by-step instructions these experts detail not only how to attach or print arrays on various matrices, but also biological sample preparation (DNA and RNA), hybridization conditions, signal detection, probe optimization, different printing technologies, and data collection and analysis (bioinformatics). Additional topics covered include genotyping, sequencing by hybridization, antisense reagents, HLA-DQA typing techniques, and gene expression analysis. Rounding out the technical presentation are three chapters that review the history of microarrays, the ethical ramifications of genetic analysis using DNA arrays, and the business aspects of biochip technologies. Forward-looking and state-of-the-art, DNA Arrays: Methods and Protocols provides all investigators engaged in biological and biomedical research the full range of effective, readily reproducible microarray techniques needed today to analyze on a large scale the many different genes and gene sequences now available from the Human Genome Project.
Note:
Description based upon print version of record.
,
DNA Microarrays -- Gel-Immobilized Microarrays of Nucleic Acids and Proteins -- Sequencing by Hybridization Arrays -- Ethical Ramifications of Genetic Analysis Using DNA Arrays -- Photolithographic Synthesis of High-Density Oligonucleotide Arrays -- Automated Genotyping Using the DNA MassArray™ Technology -- Ink-Jet-Deposited Microspot Arrays of DNA and Other Bioactive Molecules -- Printing DNA Microarrays Using the Biomek® 2000 Laboratory Automation Workstation -- Hybridization Analysis of Labeled RNA by Oligonucleotide Arrays -- Analysis of Nucleic Acids by Tandem Hybridization on Oligonucleotide Microarrays -- DNA Sequencing by Hybridization with Arrays of Samples or Probes -- Using Oligonucleotide Scanning Arrays to Find Effective Antisense Reagents -- Low-Resolution Typing of HLA-DQA1 Using DNA Microarray -- Gene Expression Analysis on Medium-Density Oligonucleotide Arrays -- Use of Bioinformatics in Arrays -- Confocal Scanning of Genetic Microarrays -- Business Aspects of Biochip Technologies.
,
English
Additional Edition:
ISBN 0-89603-822-X
Additional Edition:
ISBN 0-585-41896-9
Language:
English
Bookmarklink
