UID:
almahu_9948026401702882
Format:
1 online resource (197 p.)
ISBN:
1-281-03276-X
,
9786611032760
,
0-08-053690-5
,
1-4356-0809-7
Content:
This essential methods manual for immunohematologists (or hematologists and immunohematologists) provides information on genes that encode antigens on red blood cells, platelets and neutrophils. The book begins by covering general concepts in molecular biology and specific protocols such as DNA preparation, PCR-RFLP and allele-specific PCR. Information on the erythrocyte, platelet and neutrophil antigen systems and the molecular basis of polymorphisms are presented clearly in a gene facts sheet format. Database accession numbers and useful adjuncts such as Request forms, worksheets for PCR/enz
Note:
Description based upon print version of record.
,
Front Cover; Molecular Protocols in Transfusion Medicine; Copyright Page; Contents; Preface; List of Abbreviations; Part 1: Introduction; 1.1 Background; 1.2 Aim, Scope and Purpose of the Book; 1.3 Organization of the Book; 1.4 Terminology; 1.5 Regulatory Compliance; 1.6 References; Part 2: General Considerations; 2.1 Clinical Information; 2.2 Basic Principles of Molecular Analysis; 2.3 Troubleshooting; 2.4 References; Part 3: Protocols; 3.1 Sample Shipment and Storage; 3.2 Reagents; 3.3 Harvesting WBCs by RBC Sedimentation; 3.4 Short-term Cell Cultures from Amniotic Fluid
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3.5 Cultured Amniocyte Cell Harvesting3.6 Cell Preparation and DNA Extraction; 3.7 Assessment of DNA Quality, Quantity and Purity; 3.8 PCR Amplification; 3.9 Restriction Enzyme Digestion for PCR-RFLP Analysis; 3.10 Allele-specific PCR; 3.11 Agarose Gel Preparation and Electrophoresis; 3.12 Acrylamide Gel Preparation and Electrophoresis; 3.13 Single-strand Conformation Polymorphism (SSCP); 3.14 Optimizing a PCR; 3.15 Gene Zygosity Assessment by Quantitative PCR Amplification; 3.16 VNTR and STR Analysis by PCR Amplification; 3.17 Multiplex PCR Analysis; 3.18 Southern Analysis; 3.19 References
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Part 4: Red Cell Blood Groups4.1 Terminology; 4.2 Clinical Applications; 4.3 Situations where Genotype and Phenotype do not Agree; 4.4 References; 4.5 Facts Sheets, Gene Maps and Molecular Protocols; Part 5: Platelet Blood Groups; 5.1 Terminology; 5.2 Clinical Applications; 5.3 References; 5.4 Facts Sheets, Gene Maps and Molecular Protocols; Part 6: Neutrophil Blood Groups; 6.1 Terminology; 6.2 Clinical Applications; 6.3 References; 6.4 Facts Sheets, Gene Maps and Molecular Protocols; Part 7: Appendices; 7.1 Database Information; 7.2 Glossary; 7.3 References
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7.4 Works Sheets and Request FormsIndex; Useful Tables
,
English
Additional Edition:
ISBN 0-12-209370-4
Language:
English
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