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Format: 1 Online-Ressource.

ISBN: 978-3-662-67273-0

Additional Edition: Erscheint auch als Druck-Ausgabe, Hardcover ISBN 978-3-662-67272-3

Additional Edition: Erscheint auch als Druck-Ausgabe, Paperback ISBN 978-3-662-67275-4

Language: English

DOI: 10.1007/978-3-662-67273-0

URL: Volltext  (kostenfrei)

URL: Volltext  (kostenfrei)

UID:

Format: 1 Online-Ressource.

ISBN: 978-3-662-67273-0

Additional Edition: Erscheint auch als Druck-Ausgabe, Hardcover ISBN 978-3-662-67272-3

Additional Edition: Erscheint auch als Druck-Ausgabe, Paperback ISBN 978-3-662-67275-4

Language: English

DOI: 10.1007/978-3-662-67273-0

URL: Volltext  (kostenfrei)

URL: Volltext  (kostenfrei)

UID:

Format: 1 Online-Ressource

ISBN: 9783662672730

Additional Edition: Erscheint auch als Druck-Ausgabe, Hardcover ISBN 978-3-662-67272-3

Additional Edition: Erscheint auch als Druck-Ausgabe, Paperback ISBN 978-3-662-67275-4

Language: English

DOI: 10.1007/978-3-662-67273-0

URL: Volltext  (kostenfrei)

URL: Volltext  (kostenfrei)

UID:

Format: 1 online resource (xx, 314 pages) : , illustrations (some color)

Edition: 1st ed. 2023.

ISBN: 3-662-67273-1

Content: This open-access book presents essential concepts and new, illustrated methods for mutation-assisted breeding of Coffea arabica L. (Arabica), one of the world's most important cash crops and beverages. Arabica coffee accounts for about 60% of the world's coffee production. Coffee leaf rust (CLR), caused by the fungus Hemileia vastatrix is the major disease affecting Arabica coffee resulting in losses of over $1 billion annually. The geographical distribution of CLR is expanding due to climate change. Moreover, the genetic improvement of Arabica coffee is constrained due to its very narrow genetic base. This protocol book covers practical methods to enhance genetic diversity in Arabica coffee through induced mutagenesis and for screening for resistance to CLR. Current breeding approaches, challenges, and opportunities for Arabica coffee improvement are briefly reviewed and a survey of common coffee diseases with emphasis on CLR is presented. Based on latest advances in science and technology, this book includes novel methods for single-cell mutagenesis using in vitro cell and tissue culture techniques and for genome-wide screening of induced mutations using genomics tools. Each protocol chapter provides step-by-step illustrated methods supported by example results. Given the impact of recent CLR epidemics on Arabica coffee production in Latin America, the book is intended to serve as a timely reference and guide for students and researchers in the agricultural sciences, plant pathologists and breeders, as well as growers and end-users interested in producing novel coffee genotypes for genetic studies, breeding, and commercial applications. .

Note: Introduction coffee breeding and challenges -- Choice of materials for mutation induction in arabica coffee -- Improved in-vitro establishment and germination of Coffea arabica seed -- Induced mutagenesis in coffee (Coffea arabica L.) using chemical agents -- Mutation induction using gamma irradiation and high frequency embryogenic callus from coffee (Coffea arabica) -- Chemical mutagenesis of Coffea arabica mature seed using EMS -- Physical mutagenesis of coffee seeds -- In-vitro regeneration of Coffea arabica var. Venecia through somatic embryogenesis -- Protocol on mutation induction in Coffea arabica using in vivo grafting and cuttings -- Protocol on mutation induction in coffee using in vitro tissue cultures -- Screening for resistance to coffee leaf rust -- Protocol to send samples of coffee leaf rust to CIFC -- Coffee leaf rust (Hemileia vastatrix) inoculation and evaluation under laboratory conditions -- Development of a PCR-Based Molecular Detection -- Technique for the Early Diagnosis of Coffee Leaf -- Rust Caused by Hemileia vastatrix -- Protocols for chromosome preparations: molecular cytogenetics and studying genome organization in coffee. .

Additional Edition: ISBN 3-662-67272-3

Language: English

DOI: 10.1007/978-3-662-67273-0

URL: kostenfrei

UID:

Format: 1 online resource (316 pages)

Edition: 1st ed.

ISBN: 9783662672730

Note: Intro -- Preface -- Acknowledgements -- Contents -- Contributors -- Introduction -- Mutation Breeding in Arabica Coffee -- 1 General Principles of Plant Mutation Breeding -- 2 Breeding Limitations in Arabica Coffee -- 3 Mutation Breeding in Arabica Coffee -- 3.1 Background -- 3.2 The Need for Radiosensitivity Testing -- 3.3 Choice of Material for Mutation Induction -- 3.4 Enabling Biotechnology and Genomics Tools -- References -- Coffee Leaf Rust Resistance: An Overview -- 1 Introduction -- 2 Coffee's Main Diseases -- 3 CLR Causal Agent: Hemileia vastatrix -- 3.1 Life Cycle and Infection Process -- 3.2 Genetic and Physiological Diversity -- 3.3 Implications of Rust Molecular Diversity and Population Dynamics in Coffee Breeding -- 4 Coffee Rust Resistance -- 4.1 Concepts of Plant Disease Resistance -- 4.2 Mechanisms of Coffee Resistance to H. vastatrix -- 5 Conclusions -- References -- Induced Mutagenesis of in Vitro Tissues and Cells of Coffea arabica L. -- In Vitro Plantlet Establishment of Coffea arabica from Cut Seed Explants -- 1 Introduction -- 2 Materials -- 2.1 Plant Material -- 2.2 Chemicals -- 2.3 Tools and Labware -- 2.4 Lab Equipment -- 3 Methods -- 3.1 Media Preparation -- 3.2 Seed Preparation -- 3.3 Explant Isolation -- 3.4 Surface Sterilization of the Cut Seed -- 3.5 Transfer of Explants and Culture Conditions -- 3.6 Germination and Plantlet Development -- 4 Notes -- References -- Somatic Embryogenesis and Temporary Immersion for Mass Propagation of Chimera-Free Mutant Arabica Coffee Plantlets -- 1 Introduction -- 2 Materials -- 2.1 Plant Material -- 2.2 Supplies, Reagents and Basic Equipment -- 2.3 Equipment -- 2.4 Stock Solutions and Tissue Culture Media -- 3 Methods -- 3.1 Media Preparation -- 3.2 Tissue Collection and Disinfection -- 3.3 In Vitro Leaf Disc Preparation and Primary Callus Induction. , 3.4 Embryogenic Callus Induction -- 3.5 Cell Multiplication and Establishing the Embryogenic Cell Suspension -- 3.6 Gamma-ray irradiation of the Embryogenic Cell Suspension -- 3.7 Somatic Embryo Differentiation and Germination -- 3.8 Development of Somatic Embryos and Conversion to Plantlets -- 4 Notes -- References -- Protocol on Mutation Induction in Coffee Using In Vitro Tissue Cultures -- 1 Introduction -- 2 Materials -- 2.1 Establishment of a Collection of Donor Material In Vivo -- 2.2 Establishment of In Vitro Shoot Cultures as Donor Material -- 2.3 Establishment of Tissue Culture Material for Mutation Induction -- 2.4 Mutagenesis by Physical Agents -- 3 Methods -- 3.1 Mutagenesis by Physical Agents -- 3.2 Selection and Treatment of Explants -- 3.3 Regeneration of Mutant Plant Lines -- 4 Notes -- References -- Mutation Induction Using Gamma-Ray Irradiation and High Frequency Embryogenic Callus from Coffee (Coffea arabica L.) -- 1 Introduction -- 2 Materials -- 2.1 Plant Material -- 2.2 Explants Collection and Disinfection -- 2.3 Induction of Embryogenic Callus -- 2.4 Embryogenic Callus Multiplication -- 2.5 Embryogenic Callus Irradiation -- 2.6 Regeneration of the Irradiated Embryogenic Callus -- 2.7 Embryo Germination -- 2.8 Development of Somatic Embryos into Plantlets -- 2.9 Media Culture: Preparation -- 3 Methods -- 3.1 Explants Collection and Disinfection -- 3.2 Induction of Embryogenic Callus -- 3.3 Embryogenic Callus Multiplication -- 3.4 Irradiation of the Embryogenic Callus -- 3.5 Regeneration of the Irradiated Embryogenic Callus -- 3.6 Embryo Germination -- 3.7 Regeneration of Plantlets -- 4 Notes -- References -- Chemical Mutagenesis of Embryogenic Cell Suspensions of Coffea arabica L. var. Catuaí Using EMS and NaN3 -- 1 Introduction -- 2 Materials -- 2.1 Plant Material -- 2.2 Reagents -- 2.3 Glassware and Minor Equipment. , 2.4 Equipment -- 2.5 Tissue Culture Media -- 2.6 Software -- 3 Methods -- 3.1 Preparation of Stock Solutions -- 3.2 Preparation of Tissue Culture Media -- 3.3 Germination of Coffee Zygotic Embryos Under In Vitro Conditions -- 3.4 Protocol for Plant Regeneration via Somatic Embryogenesis -- 3.5 Determination of the Viability of the Embryogenic Calli -- 3.6 Sodium Azide Dose Determination -- 3.7 Ethyl Methanesulphonate Dose Determination -- 3.8 Bulk Mutagenesis -- 4 Notes -- References -- Chemical Mutagenesis of Coffea arabica L. var. Venecia Cell Suspensions Using EMS -- 1 Introduction -- 2 Materials -- 2.1 Culture Medium -- 2.2 Chemical Toxicity Test -- 2.3 Calculation of Lethal Dose (LD) -- 3 Methods -- 3.1 Preparation of Liquid Culture Medium -- 3.2 Mutagenesis of Coffee Cell Suspensions: Chemical Toxicity Test -- 3.3 Calculation of Lethal Dose (LD) -- 3.4 Development of Somatic Embryos and Conversion into Plantlets -- 4 Notes -- References -- Chemical Mutagenesis of Zygotic Embryos of Coffea arabica L. var. Catuaí Using EMS and NaN3 -- 1 Introduction -- 2 Materials -- 2.1 Plant Material -- 2.2 Reagents -- 2.3 Glassware and Minor Equipment -- 2.4 Equipment -- 2.5 Software -- 2.6 Bulk Mutagenesis of Zygotic Embryos -- 3 Methods -- 3.1 Preparation of Stock Solutions -- 3.2 Preparation of Tissue Culture Medium -- 3.3 Seed Disinfection and Excision of Zygotic Embryos -- 3.4 Determination of the Viability of the Zygotic Embryos -- 3.5 Sodium Azide Dose Determination -- 3.6 Ethyl Methanesulphonate Dose Determination -- 3.7 Bulk Mutagenesis -- 3.8 Acclimatization of M1 Plantlets -- 4 Notes -- References -- Induced Mutagenesis of Seed and Vegetative Propagules of Coffea arabica L. -- Physical Mutagenesis of Arabica Coffee Seeds and Seedlings -- 1 Introduction -- 2 Materials -- 3 Methods -- 3.1 Seed Treatment -- 3.2 Coffee Seedlings Treatment. , 3.3 Example Radio-Sensitivity Testing of Arabica Coffee Seed Under Laboratory Conditions -- 4 Notes -- References -- Mutation Induction in Coffea arabica L. Using in Vivo Grafting and Cuttings -- 1 Introduction -- 2 Materials -- 2.1 Grafting -- 2.2 Cutting -- 3 Methods -- 3.1 Mutagenesis and Propagation of Coffee Through Bud Grafting -- 3.2 Mutagenesis and Propagation of Coffee Through Cuttings -- 4 Notes -- References -- Chemical Mutagenesis of Mature Seed of Coffea arabica L. var. Venecia Using EMS -- 1 Introduction -- 2 Materials -- 2.1 Chemical Toxicity Test -- 2.2 Calculation of Lethal Dose (LD) and Growth Reduction (GR) -- 2.3 Bulk Mutagenesis -- 2.4 Phenotyping -- 3 Methods -- 3.1 Seed Quality Control and Pre-treatment -- 3.2 Coffee Seed Mutagenesis -- 3.3 Post-treatment Handling of Seeds -- 3.4 Calculation of Lethal Dose (LD): Data Collection and Analyses -- 3.5 Bulk Mutagenesis -- 3.6 Phenotyping -- 4 Notes -- References -- Chemical Mutagenesis of Coffee Seeds (Coffea arabica L. var. Catuaí) Using NaN3 -- 1 Introduction -- 2 Materials -- 2.1 Plant Material -- 2.2 Reagents -- 2.3 Glassware and Minor Equipment -- 2.4 Equipment -- 2.5 Software -- 3 Methods -- 3.1 Preparation of Stock Solutions -- 3.2 Seed Germination Test -- 3.3 Sodium Azide Toxicity Test and Dose Optimization -- 3.4 Bulk Mutagenesis -- 3.5 Planting Mutagenized M1 Seedling -- 4 Notes -- References -- Mutant Phenotyping and CLR Resistance Screening Methods -- Use of Open-Source Tools for Imaging and Recording Phenotypic Traits of a Coffee (Coffea arabica L.) Mutant Population -- 1 Introduction -- 2 Materials -- 2.1 Establishment of the C. Arabica Mutant Populations -- 2.2 Phenotyping of the Mutant Populations -- 2.3 Data Analysis Tools -- 3 Methods -- 3.1 Establishing the M1 Mutant Population -- 3.2 Phenotypic Characterisation of the M1 Mutant Population. , 3.3 Image Analysis to Estimate the Canopy Size -- 3.4 Statistical Analysis -- 3.5 Establishment of M2 Population -- 4 Notes -- References -- Screening for Resistance to Coffee Leaf Rust -- 1 Introduction -- 2 Materials -- 2.1 To Collect and Store Inoculum -- 2.2 Spores Viability -- 2.3 Inoculation -- 2.4 Phenotyping of Coffee-Rust Interactions -- 3 Methods -- 3.1 Procedures to Collect and Store Inoculum -- 3.2 Spores Germination Tests -- 3.3 Inoculation Techniques -- 3.4 Phenotypic Scoring Method for Disease Resistance -- 4 Notes -- References -- Inoculation and Evaluation of Hemileia vastatrix Under Laboratory Conditions -- 1 Introduction -- 2 Materials -- 2.1 Preparation of Rust Inoculum -- 2.2 Rust Inoculation -- 2.3 Rust Evaluation -- 3 Methods -- 3.1 Preparation of Rust Inocula -- 3.2 Rust Inoculation -- 3.3 Rust Evaluation -- 4 Notes -- References -- Evaluation of Coffee (Coffea arabica L. var. Catuaí) Tolerance to Leaf Rust (Hemileia vastatrix) Using Inoculation of Leaf Discs Under Controlled Conditions -- 1 Introduction -- 2 Materials -- 2.1 Plant Material -- 2.2 Other Biological Materials -- 2.3 Consumables and Minor Equipment -- 2.4 Reagents and Agrochemicals -- 2.5 Equipment -- 3 Methods -- 3.1 Germination of M2 Seeds -- 3.2 Planting Seedlings M2 -- 3.3 Preparation of Coffee Leaf Rust Inoculum -- 3.4 Inoculation of the Coffee Leaf Discs with CLR -- 3.5 Evaluation of Plant Resistance Against CLR -- 4 Notes -- References -- A PCR-Based Assay for Early Diagnosis of the Coffee Leaf Rust Pathogen Hemileia vastatrix -- 1 Introduction -- 2 Materials -- 3 Methods -- 3.1 Designing the Specific Primers for Hemileia vastatrix -- 3.2 Total DNA Extraction from Suspected Diseased Leaves or Typical Diseased Samples -- 3.3 Preparation of the PCR Reaction Mixture and PCR Amplification -- 3.4 Gel Electrophoresis -- 4 Notes -- References. , Molecular Characterisation of Induced Mutations in Coffee.

Additional Edition: Print version: Ingelbrecht, Ivan L. W. Mutation Breeding in Coffee with Special Reference to Leaf Rust Berlin, Heidelberg : Springer Berlin / Heidelberg,c2023 ISBN 9783662672723

Language: English

Keywords: Electronic books.

URL: Click to View

UID:

Format: 1 online resource (xx, 314 pages) : , illustrations (some color)

Edition: 1st ed. 2023.

ISBN: 3-662-67273-1

Content: This open-access book presents essential concepts and new, illustrated methods for mutation-assisted breeding of Coffea arabica L. (Arabica), one of the world's most important cash crops and beverages. Arabica coffee accounts for about 60% of the world's coffee production. Coffee leaf rust (CLR), caused by the fungus Hemileia vastatrix is the major disease affecting Arabica coffee resulting in losses of over $1 billion annually. The geographical distribution of CLR is expanding due to climate change. Moreover, the genetic improvement of Arabica coffee is constrained due to its very narrow genetic base. This protocol book covers practical methods to enhance genetic diversity in Arabica coffee through induced mutagenesis and for screening for resistance to CLR. Current breeding approaches, challenges, and opportunities for Arabica coffee improvement are briefly reviewed and a survey of common coffee diseases with emphasis on CLR is presented. Based on latest advances in science and technology, this book includes novel methods for single-cell mutagenesis using in vitro cell and tissue culture techniques and for genome-wide screening of induced mutations using genomics tools. Each protocol chapter provides step-by-step illustrated methods supported by example results. Given the impact of recent CLR epidemics on Arabica coffee production in Latin America, the book is intended to serve as a timely reference and guide for students and researchers in the agricultural sciences, plant pathologists and breeders, as well as growers and end-users interested in producing novel coffee genotypes for genetic studies, breeding, and commercial applications. .

Note: Introduction coffee breeding and challenges -- Choice of materials for mutation induction in arabica coffee -- Improved in-vitro establishment and germination of Coffea arabica seed -- Induced mutagenesis in coffee (Coffea arabica L.) using chemical agents -- Mutation induction using gamma irradiation and high frequency embryogenic callus from coffee (Coffea arabica) -- Chemical mutagenesis of Coffea arabica mature seed using EMS -- Physical mutagenesis of coffee seeds -- In-vitro regeneration of Coffea arabica var. Venecia through somatic embryogenesis -- Protocol on mutation induction in Coffea arabica using in vivo grafting and cuttings -- Protocol on mutation induction in coffee using in vitro tissue cultures -- Screening for resistance to coffee leaf rust -- Protocol to send samples of coffee leaf rust to CIFC -- Coffee leaf rust (Hemileia vastatrix) inoculation and evaluation under laboratory conditions -- Development of a PCR-Based Molecular Detection -- Technique for the Early Diagnosis of Coffee Leaf -- Rust Caused by Hemileia vastatrix -- Protocols for chromosome preparations: molecular cytogenetics and studying genome organization in coffee. .

Additional Edition: ISBN 3-662-67272-3

Language: English

DOI: 10.1007/978-3-662-67273-0

UID:

Format: 1 online resource (xx, 314 pages) : , illustrations (some color)

Edition: 1st ed. 2023.

ISBN: 3-662-67273-1

Content: This open-access book presents essential concepts and new, illustrated methods for mutation-assisted breeding of Coffea arabica L. (Arabica), one of the world's most important cash crops and beverages. Arabica coffee accounts for about 60% of the world's coffee production. Coffee leaf rust (CLR), caused by the fungus Hemileia vastatrix is the major disease affecting Arabica coffee resulting in losses of over $1 billion annually. The geographical distribution of CLR is expanding due to climate change. Moreover, the genetic improvement of Arabica coffee is constrained due to its very narrow genetic base. This protocol book covers practical methods to enhance genetic diversity in Arabica coffee through induced mutagenesis and for screening for resistance to CLR. Current breeding approaches, challenges, and opportunities for Arabica coffee improvement are briefly reviewed and a survey of common coffee diseases with emphasis on CLR is presented. Based on latest advances in science and technology, this book includes novel methods for single-cell mutagenesis using in vitro cell and tissue culture techniques and for genome-wide screening of induced mutations using genomics tools. Each protocol chapter provides step-by-step illustrated methods supported by example results. Given the impact of recent CLR epidemics on Arabica coffee production in Latin America, the book is intended to serve as a timely reference and guide for students and researchers in the agricultural sciences, plant pathologists and breeders, as well as growers and end-users interested in producing novel coffee genotypes for genetic studies, breeding, and commercial applications. .

Note: Introduction coffee breeding and challenges -- Choice of materials for mutation induction in arabica coffee -- Improved in-vitro establishment and germination of Coffea arabica seed -- Induced mutagenesis in coffee (Coffea arabica L.) using chemical agents -- Mutation induction using gamma irradiation and high frequency embryogenic callus from coffee (Coffea arabica) -- Chemical mutagenesis of Coffea arabica mature seed using EMS -- Physical mutagenesis of coffee seeds -- In-vitro regeneration of Coffea arabica var. Venecia through somatic embryogenesis -- Protocol on mutation induction in Coffea arabica using in vivo grafting and cuttings -- Protocol on mutation induction in coffee using in vitro tissue cultures -- Screening for resistance to coffee leaf rust -- Protocol to send samples of coffee leaf rust to CIFC -- Coffee leaf rust (Hemileia vastatrix) inoculation and evaluation under laboratory conditions -- Development of a PCR-Based Molecular Detection -- Technique for the Early Diagnosis of Coffee Leaf -- Rust Caused by Hemileia vastatrix -- Protocols for chromosome preparations: molecular cytogenetics and studying genome organization in coffee. .

Additional Edition: ISBN 3-662-67272-3

Language: English

DOI: 10.1007/978-3-662-67273-0

UID:

Format: 1 Online-Ressource (314 p.)

ISBN: 9783662672730 , 9783662672723

Content: This open-access book provides a comprehensive overview of current methodologies for improving resistance to leaf rust in coffee, one of the world's most important cash crops and beverages. Coffea arabica L. (Arabica) accounts for about 60% of the world's coffee production. Coffee leaf rust (CLR), caused by the fungus Hemileia vastatrix is the major disease affecting Arabica coffee resulting in losses of over $1 billion annually. The geographical distribution of CLR is expanding due to climate change. Moreover, the genetic improvement of Arabica coffee is constrained due to its very narrow genetic base. This protocol book introduces essential concepts of mutation breeding as an efficient tool to increase the genetic diversity of Arabica coffee and presents practical methods on mutation induction and screening for resistance to CLR. Current breeding approaches, challenges and opportunities for Arabica coffee improvement are briefly reviewed and a survey of common coffee diseases with emphasis on CLR is presented. Practical protocols for mutation induction and screening for resistance to CLR are described, including novel methods for single-cell mutagenesis using in vitro cell and tissue culture techniques and for genome-wide screening of induced mutations using genomics tools. Each protocol chapter has an introduction and is supported by example results. Given the impact of recent CLR epidemics on Arabica coffee production in Latin America, the book is intended to serve as a timely reference and guide for students and researchers in the agricultural sciences, plant pathologists and breeders, as well as growers and end-users interested in producing novel coffee genotypes for genetic studies, breeding, and commercial applications

Note: English

Language: Undetermined

URL: kostenfrei

UID:

Format: 1 online resource (xx, 314 pages) : , illustrations (some color)

ISBN: 9783662672730 , 3662672731

Content: This open-access book presents essential concepts and new, illustrated methods for mutation-assisted breeding of Coffea arabica L. (Arabica), one of the world's most important cash crops and beverages. Arabica coffee accounts for about 60% of the world's coffee production. Coffee leaf rust (CLR), caused by the fungus Hemileia vastatrix is the major disease affecting Arabica coffee resulting in losses of over $1 billion annually. The geographical distribution of CLR is expanding due to climate change. Moreover, the genetic improvement of Arabica coffee is constrained due to its very narrow genetic base. This protocol book covers practical methods to enhance genetic diversity in Arabica coffee through induced mutagenesis and for screening for resistance to CLR. Current breeding approaches, challenges, and opportunities for Arabica coffee improvement are briefly reviewed and a survey of common coffee diseases with emphasis on CLR is presented. Based on latest advances in science and technology, this book includes novel methods for single-cell mutagenesis using in vitro cell and tissue culture techniques and for genome-wide screening of induced mutations using genomics tools. Each protocol chapter provides step-by-step illustrated methods supported by example results. Given the impact of recent CLR epidemics on Arabica coffee production in Latin America, the book is intended to serve as a timely reference and guide for students and researchers in the agricultural sciences, plant pathologists and breeders, as well as growers and end-users interested in producing novel coffee genotypes for genetic studies, breeding, and commercial applications.

Note: Introduction coffee breeding and challenges -- Choice of materials for mutation induction in arabica coffee -- Improved in-vitro establishment and germination of Coffea arabica seed -- Induced mutagenesis in coffee (Coffea arabica L.) using chemical agents -- Mutation induction using gamma irradiation and high frequency embryogenic callus from coffee (Coffea arabica) -- Chemical mutagenesis of Coffea arabica mature seed using EMS -- Physical mutagenesis of coffee seeds -- In-vitro regeneration of Coffea arabica var. Venecia through somatic embryogenesis -- Protocol on mutation induction in Coffea arabica using in vivo grafting and cuttings -- Protocol on mutation induction in coffee using in vitro tissue cultures -- Screening for resistance to coffee leaf rust -- Protocol to send samples of coffee leaf rust to CIFC -- Coffee leaf rust (Hemileia vastatrix) inoculation and evaluation under laboratory conditions -- Development of a PCR-Based Molecular Detection -- Technique for the Early Diagnosis of Coffee Leaf -- Rust Caused by Hemileia vastatrix -- Protocols for chromosome preparations: molecular cytogenetics and studying genome organization in coffee.

Additional Edition: ISBN 3-662-67272-3

Language: English

Keywords: Electronic books.

DOI: 10.1007/978-3-662-67273-0

URL: SpringerLink

URL: Springer Nature

URL: Click here to view book

UID:

Format: 1 Online-Ressource.

ISBN: 978-3-662-67273-0

Additional Edition: Erscheint auch als Druck-Ausgabe, Hardcover ISBN 978-3-662-67272-3

Additional Edition: Erscheint auch als Druck-Ausgabe, Paperback ISBN 978-3-662-67275-4

Language: English

DOI: 10.1007/978-3-662-67273-0

URL: Volltext  (kostenfrei)

URL: Volltext  (kostenfrei)

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