Kooperativer Bibliotheksverbund

UID:

Format: 1 online resource (XI, 284 p. 58 illus., 52 illus. in color.)

Edition: 2nd ed. 2018.

ISBN: 1-4939-8697-X

Series Statement: Methods in Molecular Biology, 1842

Content: This second edition volume expands on the previous edition with updated discussions on new genetic, molecular, and cellular methods used to study somatic stem cells. The chapters in this book focus on the isolation, classification, purity, and plasticity of these stem cells in a variety of organic tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Somatic Stem Cells: Methods and Protocols, Second Edition is a valuable resource for both novice and experienced molecular biologists, developmental biologists, tissue engineers, and geneticists who are interested in stem cell research and its potentials in regenerative medicine.

Note: An Update on the Therapeutic Potential of Stem Cell -- Single-Step Plasmid Based Reprogramming of Human Dermal Fibroblasts to Induced Neural Stem Cells -- Isolation and Analysis of Mesenchymal Progenitors of the Adult Hematopoietic Niche -- Identification and Isolation of Mice and Human Hematopoietic Stem Cells -- Identification and Characterization of Hair Follicle Stem Cells -- Methods of Mesenchymal Stem Cell Homing to the Blood-Brain Barrier -- 3D Bioprinting and Stem Cells -- Characterization of Gastrospheres Using 3D Coculture System -- Markers and Methods to Study Intestinal Stem Cells -- Quantitative Analysis of Intestinal Stem Cell Dynamics using Microfabricated Cell Culture Arrays -- Detection, Labeling, and Culture of Lung Stem and Progenitor Cells -- Isolation, Characterization, and Differentiation of Mouse Cardiac Progenitor Cells -- Isolating and Characterizing Adipose-Derived Stem Cells -- Enzyme-Free Isolation of Adipose-Derived Mesenchymal Stem Cell -- Identification and Characterizations of Annulus Fibrosus-Derived Stem Cells -- The Maintenance of Tendon Stem/Progenitor Cells in Culture -- Intravital Imaging to Understand Spatiotemporal Regulation of Osteogenesis and Angiogenesis in Cranial Defect Repair and Regeneration -- Beating Heart Cells from Hair Cells -- Generation of FLIP and FLIP-FlpE Targeting Vectors for Bi-Allelic Conditional and Reversible Gene Knockouts in Mouse and Human Cells -- Analytical Platforms and Techniques to Study Stem Cells Metabolism.

Additional Edition: ISBN 1-4939-8696-1

Language: English

DOI: 10.1007/978-1-4939-8697-2

UID:

Format: 1 online resource (XII, 273 p.)

Edition: 1st ed. 2008.

ISBN: 1-60327-214-3

Series Statement: Methods in Molecular Biology, 450

Content: The knowledge gained by studying germline stem cells, the source of human and animal reproduction, may find immediate application in preserving endangered wildlife, managing commercial livestock, overcoming fertility problems in humans, and treating testicular and ovary tumors. In Germline Stem Cells, leading experts explore the parameters that define germline stem cells and the mechanisms that regulate the cell behavior in order to better isolate, characterize and maintain them. Divided into two parts, the volume begins by providing protocols for germline stem cell identification and regulation in model organisms, and concludes with detailed chapters covering current techniques involving in vitro culture and the applications of the cells. Following the highly successful Methods in Molecular Biology™ series format, each chapter includes a brief introduction to the subject, a list of necessary equipment and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Germline Stem Cells is the perfect reference for scientists exploring this particular exciting and important subdivision in the fast-paced field of stem cell research.

Note: Bibliographic Level Mode of Issuance: Monograph , Identification and Regulation of Germline Stem Cells in Model Organisms -- The Development of Germline Stem Cells in Drosophila -- Analysis of the C. elegans Germline Stem Cell Region -- Immunohistological Techniques for Studying the Drosophila Male Germline Stem Cell -- Genetic Tools Used for Cell Lineage Tracing and Gene Manipulation in Drosophila Germline Stem Cells -- Structural Polarity and Dynamics of Male Germline Stem Cells in an Insect (Milkweed Bug Oncopeltus fasciatus) -- High-Resolution Light Microscopic Characterization of Spermatogonia -- Identification and Characterization of Spermatogonial Subtypes and Their Expansion in Whole Mounts and Tissue Sections from Primate Testes -- Epigenetic Control in Male Germ Cells -- GDNF Maintains Mouse Spermatogonial Stem Cells In Vivo and In Vitro -- In Vitro Culture and Applications of Germline Stem Cells -- Ectopic Grafting of Mammalian Testis Tissue into Mouse Hosts -- Spermatogonial Stem Cell Transplantation, Testicular Function, and Restoration of Male Fertility in Mice -- Isolating Highly Pure Rat Spermatogonial Stem Cells in Culture -- Deriving Mouse Spermatogonial Stem Cell Lines -- Production of Knockdown Rats by Lentiviral Transduction of Embryos with Short Hairpin RNA Transgenes -- Testicular Germ Cell Tumors in Mice -- Study Origin of Germ Cells and Formation of New Primary Follicles in Adult Human and Rat Ovaries. , English

Additional Edition: ISBN 1-61737-880-1

Additional Edition: ISBN 1-60327-213-5

Language: English

DOI: 10.1007/978-1-60327-214-8

UID:

Format: 1 online resource (XI, 217 p. 47 illus., 41 illus. in color.)

Edition: 2nd ed. 2021.

ISBN: 1-0716-1008-2

Series Statement: Methods in Molecular Biology, 2224

Content: This fully updated edition provides selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages, and modeling human diseases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Mouse Genetics: Methods and Protocols, Second Edition delivers fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students, and postdoctoral fellows working in the various disciplines of genetics, developmental biology, mouse genetics, and modeling human diseases.

Note: Generation of Mouse Model (KI & CKO) via Easi-CRISPR -- Analysis of Gene Expression Using lacZ Reporter Mouse Lines -- Linear Density Sucrose Gradients to Study Mitoribosomal Biogenesis in Tissue-Specific Knockout Mice -- Mouse Models for Studying Hippocampal Adult Neural Stem Cell Biology -- Retina as a Model to Study In Vivo Transmission of α-Synuclein in the A53T Mouse Model of Parkinson’s Disease -- Promoting Pro-Endocrine Differentiation and Graft Maturation Following Surgical Resection of the Mouse Pancreas -- Color-Coded Imaging of Cancer and Stromal Cells Interaction in the Pancreatic Cancer Tumor Microenvironment (TME) -- Generating Ins2+/-/miR-133aTg Mice to Model miRNA-Driven Cardioprotection of Diabetic Hearts in Humans -- Generating a Podocyte-Specific FcRn Knockout Mouse -- Mouse Models of Colitis-Associated Colon Cancer -- Colon Cancer Tumor Model Base on Colonoscopy Injection -- Generation of Transgenic Fluorescent Reporter Lines for Studying Hematopoietic Development in the Mouse -- Gene Inactivation in Adult Long Term-Hematopoietic Stem Cells Using Inducible Mouse Models -- Hematological Humanization of Immune Deficient Mice -- Antisense Oligonucleotide Treatment in a Humanized Mouse Model of Duchenne Muscular Dystrophy and Highly Sensitive Detection of Dystrophin Using Western Blotting.

Additional Edition: ISBN 1-0716-1007-4

Language: English

Keywords: Llibres electrònics ; Llibres electrònics ; Llibres electrònics ; Llibres electrònics

DOI: 10.1007/978-1-0716-1008-4

UID:

Format: 1 online resource (XVI, 546 p. 106 illus., 36 illus. in color.)

Edition: 1st ed. 2012.

ISBN: 1-61779-815-0

Series Statement: Methods in Molecular Biology, 879

Content:  Stem cells are found in almost all organisms from the early stages of development to the end of life. There are several types of stem cells and all of them may prove useful for medical research; however, each of the different types has both promise and limitations. Somatic Stem Cells: Methods and Protocols presents selected genetic, molecular, and cellular techniques used in somatic stem cell research and its clinical application. Chapters focus on the isolation, characterization, purity, plasticity, and clinical uses of somatic stem cells from a variety of human and animal tissues. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Through and intuitive, Somatic Stem Cells: Methods and Protocols seeks to provides scientists with the fundamental techniques of stem cell research and its potential application in regenerative medicine.

Note: Bibliographic Level Mode of Issuance: Monograph , Current thoughts on the therapeutic potential of stem cell / , Unique FACS method to isolate stem cells in planarian / , Identification of neural stem cells in the Drosophila larval brain / , Generation and staining of intestinal stem cell lineage in adult midgut / , Developing a quantitative in vivo tissue reconstitution assay to assess the relative potency of candidate populations of mouse oesophageal epithelial cells / , Identification, isolation, and culture of intestinal epithelial stem cells from murine intestine / , Isolation and characterization of distal lung progenitor cells / , Transplantation of mouse fetal liver cells for analyzing the function of hematopoietic stem and progenitor cells / , Convenient and efficient enrichment of the CD133+ liver cells from rat fetal liver as a source of liver stem/progenitor cells / , Assessing the potential clinical utility of transplantations of neural and mesenchymal stem cells for treating neurodegenerative diseases / , Functional identification of neural stem cell-derived oligodendrocytes / , Stem/progenitor cells in murine mammary gland : isolation and functional characterization / , Reporter assay to detect transfer and targeting of miRNAs in stem cell-breast cancer co-cultures / , Isolation, culture, and osteogenic/chondrogenic differentiation of bone marrow-derived mesenchymal stem cells / , Obtaining freshly isolated and cultured mesenchymal stem cells from human adipose tissue / , Collection, processing, and banking of umbilical cord blood stem cells for transplantation and regenerative medicine / , Generation of functional islets from human umbilical cord and placenta derived mesenchymal stem cells / , Isolation and characterization of human prostate Stem/Progenitor Cells / , Isolation and expansion of adult cardiac stem/progenitor cells in the form of cardiospheres from human cardiac biopsies and murine hearts / , Isolation and differentiation of human cardiomyocyte progenitor cells into cardiomyocytes / , Isolation, expansion, and characterization of human islet-derived progenitor cells / , Isolation and characterization of resident mesenchymal stem cells in human glomeruli / , Endothelial colony-forming progenitor cell isolation and expansion / , Isolation and characterization of stem cell-enriched human and canine hair follicle keratinocytes / , Human salivary gland stem cells : isolation, propagation, and characterization / , Identification, isolation, characterization, and banking of human dental pulp stem cells / , Isolation and differentiation potential of fibroblast-like stromal cells derived from human skin / , Immortalization of human mesenchymal stromal cells with telomerase and red fluorescence protein expression / , Genetic modification of mesenchymal stem cells / , Methodology, biology and clinical applications of human mesenchymal stem cells / , In vitro production of enucleated red blood cells from hematopoietic stem and progenitor cells / , Methods for cancer stem cell detection and isolation / , Biocompatible nanoparticle labeling of stem cells and their distribution in brain / , English

Additional Edition: ISBN 1-61779-814-2

Language: English

DOI: 10.1007/978-1-61779-815-3

UID:

Format: 1 online resource (XIV, 475 p. 92 illus., 68 illus. in color.)

Edition: 1st ed. 2014.

ISBN: 1-4939-1215-1

Series Statement: Methods in Molecular Biology, 1194

Content: Mouse Genetics: Methods and Protocols provides selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages, and modeling human diseases. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, a list of necessary materials, systematic, readily reproducible methods, and a notes section, which shares tips on troubleshooting in order to avoid known pitfalls.   Comprehensive and authoritative, Mouse Genetics: Methods and Protocols promises to deliver fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students, and postdoctoral fellows working in the various disciplines of mouse biology and modeling human disease.

Note: Bibliographic Level Mode of Issuance: Monograph , Development of Pronuclear Injection-Based Targeted Transgenesis in Mice Through Cre–loxP Site-Specific Recombination -- Generation of Conditional Knockout Mice -- Generating Mouse Lines for Lineage Tracing and Knockout Studies -- Generation of an Allelic Series of Knock-In Mice Using Recombinase-Mediated Cassette Exchange (RMCE) -- Generating Chimeric Mice from Embryonic Stem Cells via Vial Coculturing or Hypertonic Microinjection -- Genetic Inducible Fate Mapping in Adult Mice Using Tamoxifen-Dependent Cre Recombinases -- Intra cytoplasmic Sperm Injection (ICSI)-Mediated Transgenesis in Mice -- Generation of Bacterial Artificial Chromosome (BAC) Transgenic Mice -- Generation of Genetically Engineered Mice by the piggyBac Transposon System -- Generation and Applications of MADM-Based Mouse Genetic Mosaic System -- Generation of Mouse Lines Conditionally Over-Expressing MicroRNA Using the Rosa26-Lox-Stop-Lox System -- In Situ Hybridization (Both Radioactive and Non-Radioactive) and Spatiotemporal Gene Expression Analysis -- Isolation and Handling of Mouse Embryonic Fibroblasts -- Generation of Induced Pluripotent Stem Cells from Mouse Adipose Tissue -- Pdx1 (GFP/w) Mice for Isolation, Characterization and Differentiation of Pancreatic Progenitor Cells -- Generation of Transgenic Mouse Fluorescent Reporter Lines for Studying Hematopoietic Development -- Analyzing Gene Function in Adult Long-Term Hematopoietic Stem Cells Using the Interferon Inducible Mx1-Cre Mouse System -- Generation of Transgenic Mice by Exploiting Spermatogonial Stem Cells In Vivo -- Methods for the Detection of Genome Instability Derived from Replication Stress in Primary Mouse Embryonic Fibroblasts -- Transgenic Nude Mice Ubiquitously-Expressing Fluorescent Proteins for Color-Coded Imaging of the Tumor Microenvironment -- Genetically Engineered Insertional Mutagenesis in Mice to Model Cancer: Sleeping Beauty -- Generating Double Knockout Mice to Model Genetic Intervention for Diabetic Cardiomyopathy in Humans -- Experimental Osteoarthritis Models in Mice -- Mouse Models and Methods for Studying Human Disease, Acute Kidney Injury (AKI) -- Mouse Models of Acute and Chronic Colitis -- Manipulation and Assessment of Gut Microbiome for Metabolic Studies. , English

Additional Edition: ISBN 1-4939-1214-3

Language: English

Keywords: Laboratory Manuals. ; Handbooks, manuals, etc. ; Laboratory Manuals. ; Handbooks, manuals, etc. ; Laboratory Manuals. ; Handbooks, manuals, etc. ; Laboratory Manuals. ; Handbooks, manuals, etc.

DOI: 10.1007/978-1-4939-1215-5

UID:

Format: XVI, 546 Seiten , Ill., graph. Darst.

ISBN: 9781617798146

Series Statement: Methods in molecular biology 879

Language: English

Keywords: Regenerative Medizin ; Krebstherapie ; Stammzelle ; Aufsatzsammlung ; Aufsatzsammlung

UID:

Format: XVI, 546 S. : , Ill., graph. Darst.

ISBN: 978-1-61779-814-6 , 1-61779-814-2

Series Statement: Methods in molecular biology 879

Language: English

Subjects: Biology

Keywords: Stammzelle ; Somazelle

URL: http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024973838&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA

UID:

Format: XIII, 407 S. : , Ill., graph. Darst.

ISBN: 978-1-4899-8064-9

Language: English

Subjects: Biology

Keywords: miRNS ; Carcinogenese ; miRNS ; Krebs ; Wachstum

UID:

Format: Online-Ressource (XIII, 407 p. 47 illus., 38 illus. in color, online resource)

ISBN: 9781489980656

Series Statement: SpringerLink

Content: miRNAs are a class of endogenous, small non-protein coding RNA molecules (approx. 22 nucleotides) which are novel post-transcriptional regulators of gene expression. Since we have hundreds of miRNAs, the major challenge is now to understand their specific biological function. In fact the experimental evidence suggests that signaling pathways could be ideal candidates for miRNA-mediated regulation. Several studies suggest that miRNAs affect the responsiveness of cells to signaling molecules such as WNT, Notch, TGF-ß and EGFR. Altered expression of particular miRNAs has been implicated in the onset and development of cancer and could be used as potential biomarkers for the disease.Recently, many studies have found miRNAs have crucial regulatory roles in Cancer stem cells (CSCs) a kind of tumor initiating cells (TICs) and dormancy. Findings also suggest that DNA methylation may be important in regulating the expression of many miRNAs in several cancer initiating cells. Several miRNAs are known to either upregulated or downregulated in CSCs when compared to non-cancerous cells from the same tissues. CSCs are a small subpopulation of cells identified in a variety of tumors and involve in self-renewal, differentiation, chemoresistance and tumorigenesis. The volume will give a comprehensive account of important advancements in the area of miRNAs and cancer.

Note: Description based upon print version of record , Preface; Contents; Contributors; Part I; The Functions and Mechanisms of MicroRNAs; Chapter 1; miRNA Biogenesis and Function; 1 The Discovery of miRNAs; 2 Understanding miRNA Biogenesis; 2.1 Canonical miRNA Biogenesis: Dicer; 2.2 Canonical miRNA Biogenesis: Drosha; 2.3 Canonical miRNA Biogenesis: Nuclear Export; 2.4 Canonical miRNA Biogenesis: Transcription; 3 Noncanonical miRNA Biogenesis; 3.1 Microprocessor-Independent miRNA Biogenesis; 3.2 Dicer-Independent miRNA Biogenesis; 4 Regulation of miRNA Biogenesis; 5 Understanding miRNA Function; 5.1 Assembly of the RNA-Induced Silencing Complex , Selection of the Functional miRNA Strand Canonical miRNA Target Recognition; 6 Noncanonical miRNA Target Recognition; 7 Mechanisms of miRNA-Mediated Gene Silencing; 8 Regulation of miRNA Function; 9 Conclusions; References; Chapter 2; MicroRNAs in Cancer Progression; 1 Cancer Overview; 2 miRNAs; 3 miRNAs as Oncogenes; 4 miRNAs as Tumor Suppressors; 5 miRNA Function in Hallmarks of Cancer; 6 miRNAs and Chemoresistance; 7 Therapeutic and Clinical Implications of miRNAs; 8 Conclusions and Future Perspectives; References; Chapter 3; MicroRNA, DNA Repair, and Cancer ; 1 Introduction , 2 MicroRNA Biogenesis, Regulation, and Cancer2.1 MiRNA Biogenesis: Core Components; 2.2 RNA Modifications; 2.3 Protein Modifications; 2.4 Protein-RNA Interactions; 2.5 Protein-Protein Interactions; 3 MicroRNAs in DNA Repair; 3.1 DNA Repair Mechanisms; 3.2 Core MMR Proteins-miR-155, miR-422a, miR-21; 3.3 NER Proteins-miR-192; 3.4 H2AX-miR-24, miR-138; 3.5 BRCA1-miR182, miR-146a; 3.6 ATM-miRNA421, miR-18a, miR-100, miR-101, miR-181; 3.7 Rad51-miR-96; 3.8 RAD52-miR-210, 373, miR-302; 4 miRNAs in Other DNA Damage Response Events; 5 Conclusions and Future Prospective; References; Part II , MicroRNAs in Stem Cells and Progression of CancerChapter 4; Role of MicroRNAs in Stem Cell Regulation and Tumorigenesis in Drosophila; 1 Introduction; 2 miRNAs in Stem Cell Regulation; 3 miRNAs in Drosophila GSCs: Self-Renewal and Differentiation; 3.1 miRNA and Female GSC ; 3.2 miRNAs and male GSC ; 4 miRNAs in Drosophila SSCs; 5 miRNAs in Tumorigenesis in Drosophila; 6 Conclusion; References; Chapter 5; MicroRNAs in Stem Cells and Cancer Stem Cells; 1 Introduction; 2 Stem Cells: Overview; 2.1 Discovery of Stem Cells; 2.2 Defining Stem Cells; 2.3 Sources of Stem Cells , 2.4 Niche in Stem Cell Regulation2.5 Self-Renewal and Differentiation of Stem Cells; 2.6 Stem Cell Quiescence; 3 Cancer Stem Cell; 3.1 The CSC hypothesis; 3.2 Emerging Evidence on the CSC Hypothesis; 3.3 Targeted Therapy for Stem-Like Cancer Cells; 4 MicroRNA; 5 Connexins; 6 Stromal Cell-Derived Factor 1/CXCL12; 6.1 Overview; 6.2 Emerging Role of CXCL12 in Cancer Biology; 6.3 CXCL12-CXCR4 Signaling Loops in Cancer; 6.4 CXCL12 in Tumor Dormancy: Pharmacological Targeting; 7 miRNA and CSC; 8 Conclusion; References; Chapter 6 , MicroRNAs in Epithelial Mesenchymal Transition and Breast Cancer Progression

Additional Edition: ISBN 9781489980649

Additional Edition: Erscheint auch als Druck-Ausgabe MicroRNA in development and in the progression of cancer New York : Springer, 2014 ISBN 9781489980649

Language: English

Subjects: Biology

Keywords: miRNS ; Carcinogenese ; miRNS ; Krebs ; Wachstum ; miRNS ; Carcinogenese ; miRNS ; Krebs ; Wachstum

DOI: 10.1007/978-1-4899-8065-6

URL: Volltext

URL: Volltext

URL: Cover

UID:

Format: 1 online resource (529 p.)

ISBN: 1-61942-092-9

Series Statement: Cancer Etiology, Diagnosis and Treatments

Note: Description based upon print version of record. , English

Additional Edition: ISBN 1-61942-088-0

Language: English