Kooperativer Bibliotheksverbund

UID:

Format: 1 online resource (408 p.)

Edition: 1st ed.

ISBN: 0-12-803118-2 , 0-12-803117-4

Note: Description based upon print version of record. , Cover; Title Page; Copyright Page; Contents; List of Contributors; About the Editor; Preface; Chapter 1 - Origin of Chinmedomics; 1.1 - Introduction; 1.2 - Application and Challenges of TCM; 1.3 - Metabolomics: An Overview; 1.4 - Process of the Serum Pharmacochemistry of TCM; 1.5 - Establishment and Evolution of Chinmedomics; 1.6 - Potential Value of Chinmedomics; 1.7 - Future Perspectives; References; Chapter 2 - Methods and Protocols of Chinmedomics; 2.1 - Introduction; 2.2 - Analytical Technologies; 2.3 - Sampling; 2.4 - Data Extraction and Analysis , 2.5 - Marker Identification and Validation2.6 - Correlation Analysis between Marker Metabolites and Absorbed Chemical Components; 2.7 - Goals for Chinmedomics; 2.7.1 - Biological Mechanisms of the TCM Syndrome; 2.7.2 - Pharmacological Effects of Formulae; 2.7.3 - Correlation Analysis of Formulae and Syndrome for Effective Substances; 2.8 - Chinmedomics for Yinchenhao Tang: A Case Study; 2.9 - Conclusions; References; Chapter 3 - Chinmedomics Advancing Drug Discovery and Development from Yinchenhao Tang; 3.1 - Introduction; 3.2 - Experimental Detail; 3.2.1 - Chemicals and Materials , 3.3.4 - Cell Test to Verify the Therapeutic Efficacy of the Correlated Chemical Components3.4 - Conclusions; Chapter 4 - Chinmedomics Approach Dissects Therapeutic Properties of ShenQiWan Acting on ShenYangXu Syndrome; 4.1 - Introduction; 4.2 - Experimental Detail; 4.2.1 - Chemicals and Materials; 4.2.2 - Metabolomics Study; 4.2.2.1 - Animal Handling; 4.2.2.2 - Sample Collection and Preparation; 4.2.2.3 - UPLC-Q-TOF-HDMS Analysis; 4.2.2.4 - Metabolic Profiling and Metabolite Analysis; 4.2.3 - Constituents Analysis In Vitro and In Vivo; 4.2.3.1 - Animals , 4.2.3.2 - Preparation of SQW Samples for Analysis , English

Language: English

Keywords: Electronic books.

URL: Click to View

UID:

Format: X, 365 S. : , Ill., graph. Darst.

ISBN: 978-1-60327-324-4

Series Statement: Methods in molecular biology 652

Note: Literaturangaben

Language: English

Subjects: Biology

Keywords: Aufsatzsammlung ; Aufsatzsammlung ; Aufsatzsammlung

URL: http://digitool.hbz-nrw.de:1801/webclient/DeliveryManager?pid=3839717&custom_att_2=simple_viewer

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Format: 1 online resource (411 pages) : , illustrations (some color), photographs

ISBN: 0-12-811148-8

Note: Includes index. , Front Cover -- Serum Pharmacochemistry of Traditional Chinese Medicine -- Serum Pharmacochemistry of Traditional Chinese Medicine: Technologies, Strategies and Applications -- Copyright -- Contents -- List of Contributors -- Preface -- 1 - Traditional Chinese Medicine: Current State, Challenges, and Applications -- 1. CURRENT STATE -- 2. APPLICATION OF TCM -- 3. THE COMING ERA OF PRECISION MEDICINE: WHAT CAN WE LEARN FROM CHINESE MEDICINE? -- 4. THE CHALLENGES OF TCM -- 5. FUTURE PERSPECTIVE -- REFERENCES -- 2 - Serum Pharmacochemistry of Traditional Chinese Medicine: Historical Development and Strategies -- 1. HISTORICAL DEVELOPMENT OF SERUM PHARMACOCHEMISTRY OF TRADITIONAL CHINESE MEDICINE -- 2. THEORETICAL FOUNDATION AND RESEARCH CATEGORY OF SPT -- 3. SPT TECHNIQUES -- 4. APPLICATION AND FUTURE PERSPECTIVES OF SPT -- 5. CONCLUSION -- REFERENCES -- 3 - Integrated Serum Pharmacochemistry of TCM and Metabolomics Strategies for Innovative Drug Discovery -- 1. INTRODUCTION -- 2. METABOLOMICS IN EFFICACY EVALUATION OF FORMULAE -- 3. INTEGRATED SERUM PHARMACOCHEMISTRY OF TCM WITH METABOLOMICS -- 4. CONCLUSION -- REFERENCES -- 4 - UPLC/MS and Its Potential in Traditional Chinese Medicine Development -- 1. INTRODUCTION -- 2. POTENTIAL POWER OF UHPLC-MS -- 3. UHPLC-MS APPROACH IN TCM -- 4. IDENTIFICATION OF CHEMICAL CONSTITUENTS IN TCM SAMPLES -- 5. QUALITATIVE AND QUANTITATIVE ANALYSIS OF TCM -- 6. PHARMACOKINETICS -- 7. UHPLC-MS-BASED METABOLITE IDENTIFICATION -- 8. METABOLOMICS APPLICATION -- 9. QUALITY CONTROL OF TCM -- 10. PHARMACOLOGICAL STUDIES -- 11. CONCLUSIONS AND FUTURE PERSPECTIVE -- REFERENCES -- 5 - Analytical Characterization of Yin-Chen-Hao-Tang by Serum Pharmacochemistry of TCM -- 1. INTRODUCTION -- 2. EXPERIMENTAL -- 2.1 CHEMICALS AND MATERIALS -- 2.2 ANIMALS -- 2.3 PREPARATION OF YINCHENHAO TANG SAMPLES FOR LC/MS ANALYSIS. , 2.4 Q-TOF/MS ANALYSIS -- 2.5 DATA PROCESSING -- 3. RESULTS AND DISCUSSIONS -- 3.1 OPTIMIZATION OF MS CONDITIONS -- 3.2 UPLC-UV-Q-TOF/MS ANALYSIS OF YCHT SAMPLES IN VITRO -- 3.3 UPLC-UV-Q-TOF ANALYSIS OF PLASMA SAMPLE AFTER ORAL YCHT -- 4. CONCLUSION -- 6 - Pharmacokinetic Strategy for Screening the Effective Components From YCHT -- 1. INTRODUCTION -- 2. EXPERIMENTAL -- 2.1 CHEMICALS AND MATERIALS -- 2.2 INSTRUMENT AND ANALYTICAL CONDITIONS -- 2.3 PHARMACOKINETIC ANALYSIS IN VIVO -- 2.3.1 Animals -- 2.3.2 Preparation of YCHT Samples -- 2.3.3 Pharmacokinetic Experiments -- 2.3.4 Compartmental Analysis -- 2.4 PATTERN-RECOGNITION ANALYSIS -- 3. RESULTS -- 4. DISCUSSION -- 5. CONCLUSION -- REFERENCES -- 7 - Synergism Effects of Active Ingredients From YCHT -- 1. INTRODUCTION -- 2. MATERIALS AND METHODS -- 2.1 MATERIALS AND REAGENTS -- 2.2 CHROMATOGRAPHIC CONDITION -- 2.3 ANIMAL HANDLING -- 2.4 SAMPLES PREPARATION -- 2.5 METHOD VALIDATION -- 2.6 PHARMACOKINETICS -- 2.7 BIOCHEMICAL ANALYSIS -- 2.8 STATISTICAL ANALYSIS -- 3. RESULTS -- 3.1 METHOD VALIDATION OF PHARMACOKINETICS -- 3.2 THERAPEUTIC EFFICACY OF COC AS INDICATED BY A RAT HI MODEL -- 3.3 COC CAUSES SYNERGISTIC EFFECTS ON PHARMACOKINETICS -- 4. DISCUSSION -- 5. CONCLUSION -- 8 - Serum Pharmacochemistry of TCM for Screening the Active Ingredients From Wen-Xin Formulae -- 1. INTRODUCTION -- 2. EXPERIMENTAL -- 2.1 PREPARATION OF WEN-XIN-FORMULA SAMPLES FOR LC-MS/MS ANALYSIS -- 2.2 ANIMAL EXPERIMENTS -- 2.3 PREPARATION OF SERUM SAMPLES -- 2.4 CHROMATOGRAPHY ANALYSIS CONDITION -- 2.5 MASS SPECTROMETRY EXPERIMENTS -- 2.6 DATA PROCESSING -- 3. RESULTS AND DISCUSSION -- 3.1 OPTIMIZATION OF LC AND MS CONDITIONS -- 3.2 UPLC-ESI-Q-TOF-MS ANALYSIS OF WEN-XIN-FORMULA IN VITRO -- 3.3 CHARACTERIZATION OF CHEMICAL CONSTITUENTS FROM WEN-XIN-FORMULA IN VIVO. , 3.4 METABOLYNX ANALYSIS OF PROTOTYPE AND METABOLITES OF WEN-XIN-FORMULA -- 4. CONCLUSION -- REFERENCES -- 9 - Characterization and Pharmacokinetic Study of Multiple Constituents From Shengmai San -- 1. INTRODUCTION -- 2. EXPERIMENTAL -- 2.1 PREPARATION OF SMS SAMPLES -- 2.2 PREPARATION OF PLASMA SAMPLES -- 2.3 PHARMACOKINETIC STUDY -- 2.4 UPLC-Q-TOF-HDMS ANALYSIS -- 2.4.1 Liquid Chromatography Conditions -- 2.4.2 Mass Spectrometry Detection -- 2.5 DATA ANALYSIS -- 2.6 METHOD VALIDATION AND DATA ANALYSIS OF PHARMACOKINETICS -- 3. RESULTS AND DISCUSSION -- 3.1 ANALYTICAL CONSIDERATION FOR UPLC-Q-TOF-HDMS -- 3.2 OPTIMIZATION OF SAMPLE PREPARATION -- 3.3 IDENTIFICATION OF CONSTITUENTS OF SMS IN VIVO BY UPLC-Q-TOF-HDMS -- 3.4 STRUCTURAL CHARACTERIZATION OF PROPOSED BIOACTIVE COMPONENTS -- 3.5 APPLICATION OF METABOLYNX TO ANALYSIS COMPOUNDS IN VIVO -- 3.6 VALIDATION OF PHARMACOKINETIC METHOD -- 3.7 PHARMACOKINETIC ANALYSIS -- 4. CONCLUSION -- REFERENCES -- 10 - Multivariate Data Processing Tools to Screen the Active Ingredients From Kai-Xin-San -- 1. INTRODUCTION -- 2. MATERIAL AND METHODS -- 2.1 PREPARATION OF KAI-XIN-SAN SAMPLES -- 2.2 ANIMALS HANDLING -- 2.3 PREPARATION OF PLASMA SAMPLES IN VIVO -- 2.4 ULTRAHIGH PERFORMANCE LIQUID CHROMATOGRAPHY CONDITION -- 2.5 MASS SPECTROMETRY CONDITION -- 2.6 MULTIVARIATE DATA PROCESSING APPROACH -- 2.7 METABOLYNX PROCESSING APPROACH FOR METABOLITES ANALYSIS -- 3. RESULTS AND DISCUSSION -- 3.1 RAPID DISCOVERY AND GLOBAL CHARACTERIZATION OF MULTIPLE CONSTITUENTS FROM KAI-XIN-SAN -- 3.2 OPTIMIZATION OF CHROMATOGRAPHIC AND MASS SPECTROMETRY CONDITIONS -- 3.3 MULTIVARIATE STATISTICAL ANALYSIS -- 3.4 METABOLITES IDENTIFIED USING THE METABOLYNX TOOL -- 4. CONCLUSION -- REFERENCES -- 11 - Serum Pharmacochemistry of TCM for Determining the Active Ingredients of Shuanghuanglian Formulae -- 1. INTRODUCTION. , 2. MATERIAL AND METHODS -- 2.1 PREPARATION OF SHUANGHUANGLIAN FORMULAE SAMPLES FOR LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS -- 2.2 PREPARATION OF SERUM SAMPLES -- 2.3 HIGH-RESOLUTION LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS -- 2.4 IMPLEMENTATION OF THE BACKGROUND-SUBTRACTION ALGORITHM -- 2.5 MULTIPLE-DATA PROCESSING APPROACH -- 3. RESULTS -- 3.1 UPLC-MS CHARACTERIZATION OF CHEMICAL CONSTITUENTS FROM SHUANGHUANGLIAN FORMULAE -- 3.2 BACKGROUND-SUBTRACTION ALGORITHM -- 3.3 MULTIVARIATE STATISTICAL ANALYSIS FOR IN VIVO IDENTIFICATION OF SHUANGHUANGLIAN FORMULAE -- 3.4 CHARACTERIZATION ANALYSIS OF PROTOTYPE CONSTITUENTS AND METABOLITES OF SHUANGHUANGLIAN FORMULAE -- 4. DISCUSSION -- 5. CONCLUSION -- REFERENCES -- 12 - Pharmacokinetic-Pharmacodynamic Study of Zhi Zhu Wan -- 1. INTRODUCTION -- 2. EXPERIMENTAL -- 2.1 CHEMICALS AND REAGENTS -- 2.2 UPLC-MS/MS ANALYSIS -- 2.2.1 Liquid Chromatography Conditions -- 2.2.2 Mass Spectrometry Detection -- 2.3 ANIMAL HANDLING -- 2.4 ESTABLISHMENT OF DETERMINING METHOD FOR HESPERETIN AND NARINGENIN -- 2.4.1 Plasma Sample Preparation -- 2.4.2 Method Validation -- 2.5 PHARMACOKINETICS -- 2.6 PHARMACODYNAMICS STUDY -- 2.6.1 Isolated Mice Intestine -- 2.6.2 Effects on Mouse Small Intestinal Propulsion -- 2.7 DATA ANALYSIS -- 3. RESULTS -- 3.1 VALIDATION OF UPLC-MS/MS METHODS -- 3.2 PHARMACOKINETIC STUDY -- 3.3 PHARMACODYNAMIC STUDY -- 4. DISCUSSION -- 5. CONCLUSION -- REFERENCES -- 13 - Identification of the Absorbed Components of Shaoyao-Gancao Decoction -- 1. INTRODUCTION -- 2. MATERIALS AND METHODS -- 2.1 PREPARATION OF SHAOYAO-GANCAO DECOCTION SAMPLES -- 2.2 ANIMAL EXPERIMENTS -- 2.3 PREPARATION OF SERUM SAMPLES -- 2.4 CHROMATOGRAPHIC ANALYSIS SYSTEM -- 2.5 MASS SPECTROMETRY CONDITIONS -- 2.6 DATA PROCESSING -- 3. RESULTS AND DISCUSSION. , 3.1 UPLC-MS CHARACTERIZATION OF CHEMICAL CONSTITUENTS FROM SHAOYAO-GANCAO DECOCTION -- 3.2 CHARACTERIZATION OF IN VIVO CONSTITUENTS FROM SHAOYAO-GANCAO DECOCTION -- a, Radix Glycyrrhizae -- b, Radix Paeoniae Alba -- Api, apiose -- Glc, β-d-glucose -- GluA, glucuronic acid -- MW, Molecular weight -- Xyl, ... -- 3.3 METABOLYNX DETECTION OF THE ABSORBED COMPONENTS -- 3.4 DETECTION AND IDENTIFICATION OF CONJUGATED METABOLITES IN RAT PLASMA -- 4. CONCLUSIONS -- REFERENCES -- 14 - Rapid Analysis of Multiple Constituents of Suanzaoren Decoction by UPLC-MS -- 1. INTRODUCTION -- 2. EXPERIMENTAL -- 2.1 SAMPLE PREPARATION -- 2.2 CHROMATOGRAPHY AND MASS SPECTROMETRY CONDITIONS -- 2.3 ESTABLISHMENT OF AN IN-HOUSE MOLECULAR FORMULA DATABASE -- 3. RESULTS AND DISCUSSION -- 3.1 OPTIMIZATION OF UPLC AND MS CONDITIONS -- 3.2 UPLC-ESI-Q-TOF-MS ANALYSIS OF SZRD -- 4. CONCLUSION -- 15 - UPLC-MS Analysis of the Chemical Constituents of Liuwei Dihuang Wan -- 1. INTRODUCTION -- 2. MATERIAL AND METHODS -- 2.1. PREPARATION OF LDW SAMPLES FOR LC-MS ANALYSIS -- 2.2. CHROMATOGRAPHIC SEPARATION -- 2.3 MASS SPECTROMETRY -- 2.4 DATA PROCESSING -- 3. RESULTS AND DISCUSSION -- 4. CONCLUSION -- REFERENCE -- 16 - Multivariate Data Analysis for Rapid Identification of Chemical Constituents of Simiao Wan -- 1. INTRODUCTION -- 2. MATERIAL AND METHODS -- 2.1 PREPARATION OF SW SAMPLES FOR LC-MS ANALYSIS -- 2.2 UHPLC CONDITION -- 2.3 HIGH-RESOLUTION MS -- 2.4 DATA PROCESSING -- 3. RESULTS AND DISCUSSION -- 3.1 OPTIMIZATION OF UHPLC-ESI-Q-TOF-MS CONDITIONS -- 3.2 UHPLC-ESI-Q-TOF-MS ANALYSIS OF SW -- 3.3 MS/MS CHARACTERIZATION OF CHEMICAL CONSTITUENTS FROM SW -- 4. CONCLUSION -- 17 - Time-Course Study of Multicomponents After Oral Administration of Stemonae Radix -- 1. INTRODUCTION -- 2. EXPERIMENTAL -- 2.1 SAMPLE PREPARATION OF STEMONAE RADIX -- 2.2 LIQUID CHROMATOGRAPHY CONDITIONS. , 2.3 MASS SPECTROMETRY DETECTION.

Additional Edition: ISBN 0-12-811147-X

Language: English

UID:

Format: 1 online resource (X, 350 p.)

Edition: 1st ed. 2010.

ISBN: 1-60327-325-5

Series Statement: Methods in Molecular Biology, 652

Content: New and exciting biological functions are still being discovered for vitamin A derivatives, including the vast number of physiological activities of retinoids. In Retinoids: Methods and Protocols, expert researchers in the field present the most recent technical tools with diverse techniques for both in vitro and in vivo studies. Combining biochemical, biophysical, and cell biological techniques, the book addresses topics such as the detection and quantitation of retinoids using HPLC, mass spectrometry, and fluorescence, fluorescence anisotropy of retinol binding protein, cell culture models for studying retinoid transport and the role of retinol in embryonic stem cell culture, as well as many other detailed procedures. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Retinoids: Methods and Protocols seeks to aid beginning and experienced researchers from widely varied fields in the search to uncover even more vital aspects of vitamin A’s impact on the human body.

Note: Bibliographic Level Mode of Issuance: Monograph , Quantification of Endogenous Retinoids -- Culture of Highly Differentiated Human Retinal Pigment Epithelium for Analysis of the Polarized Uptake, Processing, and Secretion of Retinoids -- Feeder-Independent Culture of Mouse Embryonic Stem Cells Using Vitamin A/Retinol -- In Vitro Assays of Rod and Cone Opsin Activity: Retinoid Analogs as Agonists and Inverse Agonists -- Physiological Studies of the Interaction Between Opsin and Chromophore in Rod and Cone Visual Pigments -- Measurement of the Mobility of All-Trans-Retinol with Two-Photon Fluorescence Recovery After Photobleaching -- Microfluorometric Measurement of the Formation of All-Trans-Retinol in the Outer Segments of Single Isolated Vertebrate Photoreceptors -- HPLC?/?MSN Analysis of Retinoids -- Binding of Retinoids to ABCA4, the Photoreceptor ABC Transporter Associated with Stargardt Macular Degeneration -- Fluorescence-Based Technique for Analyzing Retinoic Acid -- The Interaction Between Retinol-Binding Protein and Transthyretin Analyzed by Fluorescence Anisotropy -- Assay of Retinol-Binding Protein–Transthyretin Interaction and Techniques to Identify Competing Ligands -- Molecular Biology and Analytical Chemistry Methods Used to Probe the Retinoid Cycle -- Visualization of Retinoid Storage and Trafficking by Two-Photon Microscopy -- Reverse-Phase High-Performance Liquid Chromatography (HPLC) Analysis of Retinol and Retinyl Esters in Mouse Serum and Tissues -- Detection of Retinoic Acid Catabolism with Reporter Systems and by In Situ Hybridization for CYP26 Enzymes -- Diet in Vitamin A Research -- Experimental Approaches to the Study of A2E, a Bisretinoid Lipofuscin Chromophore of Retinal Pigment Epithelium -- Analysis of the Retinoid Isomerase Activities in the Retinal Pigment Epithelium and Retina -- Techniques to Study Specific Cell-Surface Receptor-Mediated Cellular Vitamin A Uptake. , English

Additional Edition: ISBN 1-61779-690-5

Additional Edition: ISBN 1-60327-324-7

Language: English

DOI: 10.1007/978-1-60327-325-1

UID:

Format: 1 Online-Ressource (306 p.)

ISBN: 9783039432561 , 9783039432578

Content: This book is a compilation of works covering applied mathematical methods and their applications in complex engineering problems. The methods covered in this book include modeling and simulation, computation, analysis, control, optimization, data science, and machine learning. The engineering application topics include but are not limited to environmental engineering, cybersecurity, transportation, agriculture, smart grid, and next-generation manufacturing

Note: English

Language: English

URL: kostenfrei

UID:

Format: 200 S.

Edition: 1 p'an 1 swae

Original writing edition: 1판 1쇄

Original writing title: 한국 중산층의 생활문화 /

Original writing person/organisation: 문, 숙재

Original writing publisher: 서울 : : 집문당.

ISBN: 89-303-0804-x

Series Statement: Asan Chaedan yŏn'gu ch'ongsŏ che 68 chip

Language: Korean

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Format: 452 Seiten , Illustrationen, Diagramme , 26 cm

Edition: Ch'op'an

Original writing edition: 초판

Original writing title: 사이버 의사소통과 국어교육

Original writing publisher: 서울 : 박이정

ISBN: 979-11-5848-258-9

Note: "Cyber communication in Korean education":Rückseite

Language: Korean

Subjects: Comparative Studies. Non-European Languages/Literatures , Education , Sociology

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Format: 1 DVD-Video (115 min) , farbig , 12 cm

Uniform Title: Hei yan quan 〈Bengali, chines., malaiisch〉

Note: Original: Malaysia, China, Taiwan, Frankreich, Österreich 2006 , Bildformat 16:9 (anamorph) , Special features: interview with director Tsai Ming-Liang ; stills galleries , Taiwanisch, Malaiisch, Mandarin, Bengali - Untertitel: englisch

Language: Chinese

Subjects: General works

Keywords: Kuala Lumpur ; Soziale Situation ; Armut ; Luftverschmutzung ; Film ; DVD-Video

Author information: Cai, Mingliang 1957-

UID:

Format: 267 S , Ill., graph. Darst., Kt , 23 cm

ISBN: 9788993739411

Uniform Title: Chungguk ŭi t'albukcha kangje songhwan ŭro inhan inkwŏn ch'imhae silt'ae wa kaesŏn pangan. 〈engl.〉

Language: English

Subjects: Political Science

Keywords: China ; Nordkoreanischer Flüchtling ; Repatriierung ; Menschenrecht

UID:

Format: 1 electronic resource (306 p.)

Content: This book is a compilation of works covering applied mathematical methods and their applications in complex engineering problems. The methods covered in this book include modeling and simulation, computation, analysis, control, optimization, data science, and machine learning. The engineering application topics include but are not limited to environmental engineering, cybersecurity, transportation, agriculture, smart grid, and next-generation manufacturing.

Note: English

Additional Edition: ISBN 3-03943-256-7

Additional Edition: ISBN 3-03943-257-5

Language: English