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  • 1
    Book
    Book
    Dynamische Ermüdungsuntersuchungen an mikromechanischen Polysiliziumstrukturen (2007)
    Berlin : Univ.-Verl. der TU, Univ.-Bibliothek
    Details
    UID:
    b3kat_BV023010602
    Format: X, 131 S. , Ill., graph. Darst. , 21 cm, 208 gr.
    Edition: 1. Aufl.
    ISBN: 9783798320284 , 3798320284
    Note: Zugl.: Berlin, Techn. Univ., Diss., 2006
    Language: German
    Keywords: MEMS ; Materialermüdung ; Silicium ; Finite-Elemente-Methode ; Oxidschicht ; Hochschulschrift ; Hochschulschrift
    URL: Abstract
    URL: Inhaltsverzeichnis
    Author information: Xiao, Wei
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  • 2
    Online Resource
    Online Resource
    Yeast Protocols / (2014)
    New York, NY :Springer New York :
    Details
    UID:
    almahu_9949251674102882
    Format: 1 online resource (X, 313 p. 57 illus., 27 illus. in color.)
    Edition: 3rd ed. 2014.
    ISBN: 1-4939-0799-9
    Series Statement: Methods in Molecular Biology, 1163
    Content: Yeast Protocols, Third Edition presents up-to-date advances in research using yeasts as models. Chapters cover topics such as basic protocols in yeast culture and genomic manipulation, protocols that study certain organelles such as mitochondria and peroxisomes and their functions in autophagy, and assays commonly used in yeast-based studies that can be adapted to other organisms. As the first sequenced living organism, budding yeast S. cerevisiae and other model yeasts have helped greatly in life science research. The easy switch between the haploid and diploid state makes yeast a paradigm of genetic manipulation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Yeast Protocols, Third Edition seeks to serve both professionals and novices with newly-developed protocols to study this essential model organism.
    Note: Bibliographic Level Mode of Issuance: Monograph , Basic Investigations in Saccharomyces cerevisiae -- Isolation of Yeast Nucleic Acids -- Quantification of GFP Signals by Fluorescent Microscopy and Flow Cytometry -- Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method -- Targeted Gene Deletion in Saccharomyces cerevisiae and Schizosaccharomyces pombe -- See & Catch Method for Studying Protein Complexes in Yeast Cells: A Technique Unifying Fluorescence Microscopy and Mass Spectrometry -- Yeast Two Hybrid Liquid Screening -- Targeted Mutagenesis of a Specific Gene in Yeast -- Fluorescence Staining of Mitochondria for Morphology Analysis in S. cerevisiae -- Assays for Autophagy I: the Cvt Pathway and Non-Selective Autophagy -- Assays for Autophagy II: Mitochondrial Autophagy -- Biochemically Characterizing the Subcellular Localization of Peroxisomal Proteins by Fractionation, Protease Protection and Carbonate Extraction -- Yeast Survival and Growth Assays -- Spontaneous Mutagenesis Assay -- Mutational Specificity Analysis: Assay for Mutations in the Yeast SUP4-o Gene -- piggyBac Transposon-Based Insertional Mutagenesis for the Fission Yeast Schizosaccharomyces pombe -- Replicative and Chronological Life Span Assays -- Detection of Protein Arginine Methylation in Saccharomyces cerevisiae -- Detection of Protein Post-translational Modifications from Whole Cell Extracts in Saccharomyces cerevisiae -- Ligand Engineering Using Yeast Surface Display -- Screening Combinatorial Libraries of Cyclic Peptides Using the Yeast Two-Hybrid Assay. , English
    Additional Edition: ISBN 1-4939-0798-0
    Language: English
    Keywords: Laboratory Manuals. ; Laboratory Manuals. ; Laboratory Manuals. ; Laboratory Manuals.
    DOI: 10.1007/978-1-4939-0799-1
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  • 3
    Online Resource
    Online Resource
    Yeast Protocols / (2021)
    New York, NY :Springer US :
    Details
    UID:
    almahu_9949251335302882
    Format: 1 online resource (XI, 260 p. 55 illus., 44 illus. in color.)
    Edition: 4th ed. 2021.
    ISBN: 1-0716-0868-1
    Series Statement: Methods in Molecular Biology, 2196
    Content: This fourth edition provides all new methods on yeast that have not been included in the past three editions. Divided in four parts, chapters guide readers through general applications for genetic manipulation in yeast cells, genome-wide studies, systematically rearrange the yeast chromosomes, and pathway methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Yeast Protocol, Fourth Edition aims to compliment previous editions while offering new tools for the yeast research community. .
    Note: Use of Yeast Plasmids - Transformation and Inheritance Assays -- Construction of Tight Conditional Mutants Using the Improved Auxin-inducible Degron (iAID) Method in the Budding Yeast Saccharomyces cerevisiae -- CRISPR Nickase-Mediated Base Editing in Yeast -- Genomic Promoter Shuffling by Using Recyclable Cassettes -- Study Essential Gene Functions by Plasmid Shuffling -- Scarless Genomic Protein Labeling in Saccharomyces cerevisiae -- High-Copy Yeast Library Construction and High-Copy Rescue Genetic Screen in Saccharomyces cerevisiae -- Genome-Wide Imaging-Based Phenomic Screening Using Yeast (Saccharomyces cerevisiae) Strain Collections -- Applications of Oxford Nanopore Sequencing in Schizosaccharomyces pombe -- Measuring Genome-Wide Nascent Nucleosome Assembly Using ReIN-Map -- Targeted Integration of Complex Genetic Elements at Multi-Copy Loci by Golden Gate Assembly -- Chromosomal Rearrangements of Synthetic Yeast by SCRaMbLE -- Use YeastFab to Construct Genetic Parts and Multicomponent Pathways for Metabolic Engineering -- A Versatile Protocol to Generate Translocations in Yeast Genomes using CRISPR/Cas9 -- YeastNucleoplasmic Extracts and an Application to Visualize Chromatin Assembly on Single Molecules of DNA -- Assays for Autophagy III: Observing Dynamic Protein Trafficking -- Isolation of Aged Yeast Cells Using Biotin-Streptavidin Affinity Purification -- High-Throughput Rapid Yeast Chronological Lifespan Assay -- Long-Term Imaging and Dynamic Analysis of Cytoophidia in Yeast -- Monitoring 5’-end Resection at Site-specific Double-strand Breaks by Southern Blot Analysis. .
    Additional Edition: ISBN 1-0716-0867-3
    Language: English
    DOI: 10.1007/978-1-0716-0868-5
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  • 4
    Online Resource
    Online Resource
    Advances in Mechanical and Power Engineering : Selected Papers from The International Conference on Advanced Mechanical and Power Engineering (CAMPE 2021), October 18-21, 2021 (2023)
    Cham : Springer International Publishing | Cham : Springer
    Details
    UID:
    b3kat_BV048603589
    Format: 1 Online-Ressource (XVI, 378 p. 169 illus., 100 illus. in color)
    Edition: 1st ed. 2023
    ISBN: 9783031184871
    Series Statement: Lecture Notes in Mechanical Engineering
    Additional Edition: Erscheint auch als Druck-Ausgabe ISBN 978-3-031-18486-4
    Additional Edition: Erscheint auch als Druck-Ausgabe ISBN 978-3-031-18488-8
    Language: English
    DOI: 10.1007/978-3-031-18487-1
    URL: Volltext  (URL des Erstveröffentlichers)
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  • 5
    Online Resource
    Online Resource
    Epistemology of news frame / (2020)
    London ; : Routledge,
    Details
    UID:
    almahu_9949385895402882
    Format: 1 online resource
    ISBN: 9781003003922 , 1003003923 , 9781000759167 , 1000759164 , 9781000758795 , 1000758796 , 9781000758429 , 1000758427
    Series Statement: China perspectives in media & communication
    Content: ""Frame Analysis" has long been an active field in journalism and communication, but there are many chaotic, ambiguous definitions and duplicated studies. This book combines subjective philosophy with empirical research to fully explore what news framing is and how a media organization's news frame is constructed. Topics discussed include connotation and composition, facts and sources, functions and effects, construction and updates, competition and negotiation, presenting as a whole a clear and systematic epistemological framework and providing inspiration for news frame researchers, media practitioners and the public to understand the role of the news media. In addition, the book also examines and analyses empirical cases from different countries and regions, including particular emphasis on frame analysis in China, which can help foreign readers better understand Chinese media reports"--
    Note: Introduction: News Frame, Communication Subject and Reality Construction -- Literature Review and Research Approach -- Connotation and Composition of News Frame -- Source of News Frame -- Function and Effect of News Frame -- Construction and Update of News Frames -- News Frames Interaction: Competition and Negotiation Between Subjects -- Discussion on the News Framework from Different Perspectives -- Conclusion.
    Additional Edition: Print version: Xiao, Wei, 1971- Epistemology of news frame. London ; New York : Routledge, 2020 ISBN 9780367435349
    Language: English
    Keywords: Electronic books. ; Electronic books.
    URL: https://www.taylorfrancis.com/books/9781003003922
    URL: lizenzpflichtig
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    Stabi Berlin
    HU Berlin
  • 6
    Online Resource
    Online Resource
    Jherings Methode und Jherings Dogmatik : ein Vergleich (2024)
    Baden-Baden :Nomos,
    Details
    UID:
    almafu_BV049692894
    Format: 1 Online-Ressource (548 Seiten).
    ISBN: 978-3-7489-1665-9
    Series Statement: Rheinische Schriften zur Rechtsgeschichte Band 28
    Note: Dissertation Universität Köln 2023/2024
    Additional Edition: Erscheint auch als Druck-Ausgabe ISBN 978-3-7560-0917-6
    Language: German
    Keywords: Hochschulschrift ; Hochschulschrift
    DOI: 10.5771/9783748916659
    URL: Volltext  (URL des Erstveröffentlichers)
    URL: lizenzpflichtig
    URL: lizenzpflichtig
    URL: Inhaltstext
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  • 7
    Book
    Book
    Blockchain chicken farm : and other stories of tech in China's countryside (2020)
    New York :Farrar, Straus and Giroux,
    Details
    UID:
    almafu_BV047046462
    Format: 248 Seiten.
    Edition: First edition
    ISBN: 978-0-374-53866-8
    Series Statement: FSG Originals X Logic
    Content: "From FSGO x Logic: stories about rural China, food, and tech that reveal new truths about the globalized world"--
    Note: Includes bibliographical references and index
    Language: English
    Subjects: Economics , Ethnology
    RVK:
    QG 860
    RVK:
    LB 18440
    URL: Inhaltsverzeichnis
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  • 8
    Book
    Book
    Blockchain Hühnerfarm : Geschichten über Technologie und KI im ländlichen China (2023)
    Zürich :Diaphanes,
    Details
    UID:
    almahu_BV048991088
    Format: 263 Seiten.
    ISBN: 978-3-0358-0565-9
    Uniform Title: Blockchain Chicken Farm and other Stories of Tech in China's Countryside
    Language: German
    Subjects: Political Science , Ethnology , Sociology
    RVK:
    MH 50086
    RVK:
    MS 1630
    RVK:
    LB 40440
    Keywords: Ländlicher Raum ; Technologie ; Künstliche Intelligenz ; Sozialer Wandel ; Ländlicher Raum ; Digitale Revolution ; Electronic Commerce ; Künstliche Intelligenz ; Künstliche Intelligenz ; Reportagensammlung ; Reportagensammlung
    URL: Inhaltsverzeichnis
    URL: Inhaltsverzeichnis
    Author information: Schulz, Sabine
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  • 9
    Online Resource
    Online Resource
    Yeast Protocols / (2006)
    Totowa, NJ :Humana Press :
    Details
    UID:
    almahu_9949251239202882
    Format: 1 online resource (406 p.)
    Edition: 2nd ed.
    ISBN: 1-280-35881-5 , 9786610358816 , 1-59259-958-3
    Series Statement: Methods in Molecular Biology, 313
    Content: In this second edition of a widely used classic laboratory manual, leading experts utilize the tremendous progress and technological advances that have occurred during the last decade to create a completely new collection of not only the major basic techniques, but also advanced protocols for yeast research. Yeast Protocols, Second Edition contains 27 optimized and readily reproducible techniques suitable for both yeast researchers and investigators who wish to use yeast to study their favorite genes from other organisms. The authors provide detailed methods for the isolation of subcellular components, including organelles and macromolecules; for the basic cellular and molecular analysis specific for yeast cells; and for the creation of conditional mutant phenotypes that lend themselves to powerful genome manipulation. Additional protocols offer advanced approaches to study genetic interactions, DNA and chromatin metabolism, gene expression, as well as the foreign genes and gene products in yeast cells. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Yeast Protocols, Second Edition offers researchers a collection of traditional and novel protocols essential for studying yeasts, as well as genes from other organisms.
    Note: Description based upon print version of record. , Basic Investigations in Saccharomyces cerevisiae -- Isolation of Nucleic Acids -- Purification of Yeast Peroxisomes -- Isolation of Yeast Plasma Membranes -- Isolation of Yeast Mitochondria -- Extraction of Yeast Lipids -- Two-Dimensional Gel Electrophoresis of Total Yeast Proteins -- Pulsed-Field Gel Electrophoresis of Budding Yeast Chromosomes -- Analysis of Yeast Lipids -- Yeast Fluorescence Microscopy -- Intracellular Expression of Recombinant Antibody Fluorescent Protein Fusions for Localization of Target Antigens in Schizosaccharomyces pombe -- Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method -- Mutagenesis -- Gene Disruption in the Budding Yeast Saccharomyces cerevisiae -- Inducible Degron and Its Application to Creating Conditional Mutants -- Synthetic Lethal Screen -- Synthetic Genetic Array Analysis in Saccharomyces cerevisiae -- Two-Dimensional Agarose Gel Analysis of DNA Replication Intermediates -- Chromatin Assembly in a Crude Fraction From Yeast Cells -- Chromatin Immunoprecipitation to Study Protein-DNA Interactions in Budding Yeast -- Isolation of Yeast Nuclei and Micrococcal Nuclease Mapping of Nucleosome Positioning -- Study of Transcriptional Regulation Using a Reporter Gene Assay -- Assessing Telomeric Phenotypes -- Controlled Expression of Recombinant Genes and Preparation of Cell-Free Extracts in Yeast -- Production of Heterologous Proteins in Yeast With the Aid of the Hsp150? Carrier -- Yeast Two-Hybrid System Screening -- Import of Precursor Proteins Into Isolated Yeast Mitochondria. , English
    Additional Edition: ISBN 1-61737-569-1
    Additional Edition: ISBN 1-58829-437-4
    Language: English
    DOI: 10.1385/1592599583
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  • 10
    Article
    Article
    Hu shan ji san juan (2016)
    Show associated volumes
    Details
    UID:
    gbv_1745141308
    Edition: 清顺治刻本
    Original writing title: 壺山集三卷
    Original writing person/organisation: 陳孝威
    ISBN: 9787546153735
    Note: The work is written in long characters
    In: Ming bie ji cong kan ; 93: Di jiu shi san ce, He fei, 2016, (2016), Seite 539-572, 9787546153735
    In: year:2016
    In: pages:539-572
    Language: Chinese
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