Kooperativer Bibliotheksverbund

Berlin Brandenburg

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  • 1
    Language: English
    In: The Journal of clinical investigation, April 2011, Vol.121(4), pp.1344-8
    Description: Pilocytic astrocytoma (PA) is the most common type of primary brain tumor in children and the second most frequent cancer in childhood. Children with incompletely resected PA represent a clinically challenging patient cohort for whom conventional adjuvant therapies are only moderately effective. This has produced high clinical demand for testing of new molecularly targeted treatments. However, the development of new therapeutics for PA has been hampered by the lack of an adequate in vivo tumor model. Recent studies have identified activation of MAPK signaling, mainly by oncogenic BRAF activation, as a hallmark genetic event in the pathogenesis of human PA. Using in vivo retroviral somatic gene transfer into mouse neural progenitor cells, we have shown here that ectopic expression of the activated BRAF kinase domain is sufficient to induce PA in mice. Further in vitro analyses demonstrated that overexpression of activated BRAF led to increased proliferation of primary mouse astrocytes that could be inhibited by treatment with the kinase inhibitor sorafenib. Our in vivo model for PA shows that the activated BRAF kinase domain is sufficient to induce PA and highlights its role as a potential therapeutic target.
    Keywords: Astrocytoma -- Etiology ; Brain Neoplasms -- Etiology ; Proto-Oncogene Proteins B-Raf -- Genetics
    ISSN: 00219738
    E-ISSN: 1558-8238
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  • 2
    Language: English
    In: Molecular & Cellular Oncology, 15 September 2014, Vol.1(3)
    Description: A high-throughput phenotypic screen in glioblastoma stem-like cells (GSCs) identified a novel molecular mechanism in which ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1) plays an important role in balancing the pool of nucleotides, thus maintaining GSCs in an undifferentiated proliferative state. This finding highlights the connection between cell cycle length and the stem-like tumor state.
    Keywords: Cell Cycle ; Differentiation ; Enpp1 ; Glioblastoma Stem-Like Cells ; High-Throughput Screen ; Phosphatase ; Medicine
    E-ISSN: 2372-3556
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  • 3
    Language: English
    In: Cancer Research, 04/15/2011, Vol.71(8 Supplement), pp.3458-3458
    ISSN: 0008-5472
    E-ISSN: 1538-7445
    Source: CrossRef
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  • 4
    In: Glia, October 2015, Vol.63(10), pp.1850-1859
    Description: Retinoic acid synthesis is impaired in glioblastoma. Most glioblastoma cultures are resistant to retinoic acid. Current retinoic acid treatment strategies in glioblastoma need to account for retinoid resistance in tumor cells.
    Keywords: Glioma ; Differentiation ; Retinoic Acid
    ISSN: 0894-1491
    E-ISSN: 1098-1136
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  • 5
    In: Journal of Pathology, September 2014, Vol.234(1), pp.23-33
    Description: Cancer cells with enhanced self‐renewal capacity influence tumour growth in glioblastoma. So far, a variety of surrogate markers have been proposed to enrich these cells, emphasizing the need to devise new characterization methods. Here, we screen a large panel of glioblastoma cultures ( = 21) cultivated under stem cell‐permissive conditions and identify several cell lines with enhanced self‐renewal capacity. These cell lines are capable of matrix‐independent growth and form fast‐growing, orthotopic tumours in mice. Employing isolation, re‐plating, and label‐retention techniques, we show that self‐renewal potential of individual cells is partitioned asymmetrically between daughter cells in a robust and cell line‐specific fashion. This yields populations of fast‐ and slow‐cycling cells, which differ in the expression of cell cycle‐associated transcripts. Intriguingly, fast‐growing cells keep their slow‐cycling counterparts in a reversible state of quiescence associated with high chemoresistance. Our results suggest that two different subpopulations of tumour cells contribute to aberrant growth and tumour recurrence after therapy in glioblastoma. Copyright © 2014 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
    Keywords: Self‐Renewal; Glioblastoma; Label Retention; Quiescence
    ISSN: 0022-3417
    E-ISSN: 1096-9896
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  • 6
    Language: English
    In: The Journal of pathology, September 2014, Vol.234(1), pp.23-33
    Description: Cancer cells with enhanced self-renewal capacity influence tumour growth in glioblastoma. So far, a variety of surrogate markers have been proposed to enrich these cells, emphasizing the need to devise new characterization methods. Here, we screen a large panel of glioblastoma cultures (n = 21) cultivated under stem cell-permissive conditions and identify several cell lines with enhanced self-renewal capacity. These cell lines are capable of matrix-independent growth and form fast-growing, orthotopic tumours in mice. Employing isolation, re-plating, and label-retention techniques, we show that self-renewal potential of individual cells is partitioned asymmetrically between daughter cells in a robust and cell line-specific fashion. This yields populations of fast- and slow-cycling cells, which differ in the expression of cell cycle-associated transcripts. Intriguingly, fast-growing cells keep their slow-cycling counterparts in a reversible state of quiescence associated with high chemoresistance. Our results suggest that two different subpopulations of tumour cells contribute to aberrant growth and tumour recurrence after therapy in glioblastoma.
    Keywords: Self-Renewal; Glioblastoma; Label Retention; Quiescence ; Brain Neoplasms -- Pathology ; Glioblastoma -- Pathology ; Neoplasm Recurrence, Local -- Pathology ; Neoplastic Stem Cells -- Pathology
    ISSN: 00223417
    E-ISSN: 1096-9896
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  • 7
    In: Brain Pathology, January 2013, Vol.23(1), pp.60-72
    Description: In various types of cancers including glioblastoma, accumulating evidence show the existence of cancer stem‐like cells (), characterized by stem cell marker expression, capability of differentiation and self‐renewal, and high potential for tumor propagation . whose expression is positively regulated by the signaling pathway, is a stem cell marker in intestinal mucosa and hair follicle in the skin. As signaling is also involved in brain development, the function of in the maintenance of brain is to be assessed. Our study showed that the transcript level was increased in . Co‐immunofluorescence staining demonstrated the co‐localization of 133‐ and ‐positive cells in glioblastoma tissue sections. Functionally, silencing of by lentiviral ‐mediated knockdown induced apoptosis in brain . Moreover, depletion led to a downregulation of cell adhesion molecule expression. In line with an important function in glioma tumorigenesis, expression increased with glioma progression and correlated with an adverse outcome. Our findings suggest that plays a role in maintenance and/or survival of brain .
    Keywords: Apoptosis ; Cancer Stem Cell ; Glioblastoma ; Glioma ; Lgr5
    ISSN: 1015-6305
    E-ISSN: 1750-3639
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  • 8
    Language: English
    In: Nature methods, August 2019, Vol.16(8), pp.750-756
    Description: The Drosophila wing disc has been a fundamental model system for the discovery of key signaling pathways and for our understanding of developmental processes. However, a complete map of gene expression in this tissue is lacking. To obtain a gene expression atlas in the wing disc, we employed single cell...
    Keywords: Gene Expression Regulation, Developmental ; Drosophila -- Genetics ; Drosophila Proteins -- Genetics ; Embryo, Nonmammalian -- Metabolism ; High-Throughput Nucleotide Sequencing -- Methods ; Single-Cell Analysis -- Methods ; Wings, Animal -- Metabolism
    ISSN: 15487091
    E-ISSN: 1548-7105
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  • 9
    Language: English
    In: Methods in molecular biology (Clifton, N.J.), 2019, Vol.1979, pp.73-85
    Description: Drop-Seq is a low-cost, high-throughput platform to profile thousands of cells by encapsualting them into individual droplets. Uniquely barcoded mRNA capture microparticles and cells are coconfined through a microfluidic device within the droplets where they undergo cell lysis and RNA hybridiztion. After breaking the droplets and pooling the hybridized particles, reverse transcription, PCR, and sequencing in single reactions allow to generate data from thousands of single-cell transcriptomes while maintaining information on the cellular origin of each transcript.
    Keywords: Cell Barcoding ; Drop-Seq ; Droplet Technology ; Microfluidics ; Single-Cell RNA-Sequencing ; Systems Biology ; Transcriptomics
    E-ISSN: 1940-6029
    Source: MEDLINE/PubMed (U.S. National Library of Medicine)
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