Format:
Online-Ressource (XIV, 398p. 60 illus., 1 illus. in color, digital)
ISBN:
9781617799402
Series Statement:
Stem Cell Biology and Regenerative Medicine
Content:
Advances in Stem Cell Research discusses recent advances in stem cell science, including therapeutic applications. This volume covers such topics as biomanufacturing iPS cells for therapeutic applications, techniques for controlling stem cell fate decisions, as well as current basic research in such areas as germ line stem cells, genomics and proteomics in stem cell research. It is a useful book for biology and clinical scientists, especially young investigators and stem cell biology students who are newly entering the world of stem cells research. The editors hope that the new knowledge and research outlined in this book will help contribute to new therapies for a wide variety of diseases that presently afflict humanity.
Note:
Description based upon print version of record
,
Advances in Stem Cell Research; Preface; Contents1; Contributors; 1 ES Cell Lines from Tetraploid Mouse Blastocysts; Abstract; 1.1…Introduction; 1.2…Results; 1.3…Discussion; 1.4…Materials and Methods; 1.4.1 Mouse Handling and Gamete Recovery; 1.4.2 Double Nuclear Transfer, ICSI, and Embryo Culture; 1.4.3 Derivation and Culture of ES Cells; 1.4.4 Alkaline Phosphatase (AP) Staining; 1.4.5 Karyotype Analysis; 1.4.6 Ploidy Analysis by FACS; 1.4.7 Embryoid Body (EB) Assay; 1.4.8 Teratoma Assay; 1.4.9 Statistical Analysis; References
,
3.1.1.1 Identification of Soluble Factors Regulating hPSC Self-Renewal3.1.1.2 Development of Fully Defined Media for hPSC Expansion; 3.1.2 Defined Substrates for hPSC Expansion; 3.1.2.1 Extracellular Matrix-Based Substrates; 3.1.2.2 Synthetic Substrates; 3.1.2.3 Mechanisms of Substrate Maintenance of Self-Renewal; 3.2…Large-Scale Production of Somatic Cells from hPSCs; 3.2.1 Embryoid Body Differentiation Processes; 3.2.2 Directed Differentiation; 3.2.2.1 Neuroepithelial Cells; 3.2.2.2 Cardiomyocytes; 3.2.2.3 Islet beta Cells; 3.2.2.4 Other Cells and Tissues
,
3.3…Scalable 3D Culture Systems for hPSC Expansion and Differentiation3.3.1 Rotary and Stirred Culture Systems; 3.3.2 Perfusion Bioreactors; 3.3.3 Expansion and Differentiation of hPSCs on Microcarriers; 3.3.4 Carrier-Free Suspension Culture of hPSCs; 3.4…Future Perspectives and Challenges; Acknowledgments; References; 4 hESC-Derived Hepatocytes; Abstract; 4.1…Introduction; 4.2…Human Embryonic Stem Cells; 4.2.1 Differentiation of Human Embryonic Stem Cells Toward the Hepatic Lineage; 4.3…Spontaneous Differentiation; 4.4…Stepwise Differentiation
,
4.4.1 Differentiation Toward Definitive Endoderm by Nodal Signaling Induction4.4.2 Hepatic InductionHepatic Induction; 4.4.3 Maturation; 4.5…Differentiation Toward Liver Precursor Cells; 4.6…Oxygen Pressure and Mitochondrial Function in Hepatocyte Differentiation; 4.7…Characterization and Functional Evaluation of Differentiated Hepatocytes; 4.8…In Vivo Studies; 4.9…Small Molecules; 4.10…Epigenetics; 4.11…The Tumorigenicity of Human Embryonic Stem Cells; 4.12…MicroRNAs During Hepatocyte Differentiation; 4.13…Future Directions and Challenges; References
,
5 Advances in Induced Pluripotent Stem Cell Biology
,
2 The Significance of Culture Adaptation of Embryonic Stem Cells for Regenerative MedicineAbstract; 2.1…The Principles of Culture Adaptation; 2.2…Culture Adaptation and Cancer; 2.3…Culture Adaptation and Regenerative Medicine; 2.4…Identification of Culture Adapted ES Cells; 2.5…Can Culture Adapted Cells be Utilised in Regenerative Medicine?; 2.6…Culture Adaptation as a Tool to Study Stem Cell Fate; 2.7…Summary; References; 3 Biomanufacturing Human Pluripotent Stem Cells for Therapeutic Applications; Abstract; 3.1…Development of Defined Culture Systems; 3.1.1 Defined Media for hPSC Expansion
Additional Edition:
ISBN 9781617799396
Additional Edition:
Buchausg. u.d.T. Advances in stem cell research New York : Humana Press, 2012 ISBN 9781617799396
Language:
English
Subjects:
Biology
Keywords:
Stammzelle
;
Stammzelle
;
Aufsatzsammlung
DOI:
10.1007/978-1-61779-940-2
Bookmarklink
