New Phytologist, Sept, 2010, Vol.187, p.1058(17)
To authenticate to the full-text of this article, please visit this link: http://dx.doi.org/10.1111/j.1469-8137.2010.03346.x Byline: Robert Szczesny, Daniela Buttner, Lucia Escolar, Sebastian Schulze, Anja Seiferth, Ulla Bonas Keywords: AvrRxv; bacterial spot disease; effectors; pepper; protease; transacetylase; YopJ Abstract: Summary Pathogenicity of the Gram-negative plant pathogen Xanthomonas campestris pv. vesicatoria (Xcv) depends on a type III secretion system that translocates a cocktail of 〉 25 type III effector proteins into the plant cell. In this study, we identified the effector AvrBsT as a suppressor of specific plant defense. AvrBsT belongs to the YopJ/AvrRxv protein family, members of which are predicted to act as proteases and/or acetyltransferases. AvrBsT suppresses the hypersensitive response (HR) that is elicited by the effector protein AvrBs1 from Xcv in resistant pepper plants. HR suppression occurs inside the plant cell and depends on a conserved predicted catalytic residue of AvrBsT. Yeast two-hybrid based analyses identified plant interaction partners of AvrBs1 and AvrBsT, including a putative regulator of sugar metabolism, SNF1-related kinase 1 (SnRK1), as interactor of AvrBsT. Intriguingly, gene silencing experiments revealed that SnRK1 is required for the induction of the AvrBs1-specific HR. We therefore speculate that SnRK1 is involved in the AvrBsT-mediated suppression of the AvrBs1-specific HR. Article History: Received: 29 April 2010Accepted: 18 May 2010 Article note: Author for correspondence:, Ulla Bonas, Tel: +49 345 5526290, Email: email@example.com
Proteases -- Physiological Aspects ; Carbohydrate Metabolism -- Physiological Aspects ; Glucose Metabolism -- Physiological Aspects ; Bacterial Infections -- Physiological Aspects ; Cytological Research -- Physiological Aspects ; Proteins -- Physiological Aspects
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