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  • 1
    Language: English
    In: Immuno-analyse et biologie specialisee, June, 2012, Vol.27(3), p.112(5)
    Description: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.immbio.2012.02.005 Byline: A. Bousfiha, L. Aarab Keywords: Viande de poulet; IgE specifique; Traitement thermique; Allergie au poulet; Blanc d'Auf Abstract: The aim is to assess the sensitivity of the population of Fez and Casablanca in Morocco to chicken meat and the effect of heating on this sensitivity. The work is based on a serum bank consisting of 146 sera from patients with suspected allergy. The evaluation of specific IgE to chicken meat showed that 46% of children and 38% of adults have positive values with 12.3% of children and 10% of adults have a higher rate than 20UI/mL. This high sensitivity shows only partial cross-recognition to the egg whites ranging from 16 to 60%. Heat treatment of meat proteins in chicken showed that more than 80% of these patients show a decrease in the recognition of proteins above 30% after 1hour at 80[degrees]C. This demonstrates a high thermolability of epitopes recognized by sera from these patients indicating conformational antigenic sites rather than sequential. Author Affiliation: Unite d'immunologie et de pharmacologie, laboratoire des molecules bioactives (LMBSF), faculte des sciences et techniques, route d'Immouzer, BP 2202, Fes, Maroc Article History: Received 25 October 2011; Accepted 21 February 2012
    Keywords: Immunoglobulin E ; Proteins ; Meat ; Chicken (Meat) ; Antigenic Determinants ; Dressed Poultry ; Immunoglobulins
    ISSN: 0923-2532
    Source: Cengage Learning, Inc.
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  • 2
    Language: English
    In: Proceedings of the National Academy of Sciences of the United States of America, 14 June 2016, Vol.113(24), pp.6713-8
    Description: Principal component analysis (PCA), homozygosity rate estimations, and linkage studies in humans are classically conducted through genome-wide single-nucleotide variant arrays (GWSA). We compared whole-exome sequencing (WES) and GWSA for this purpose. We analyzed 110 subjects originating from different regions of the world, including North Africa and the Middle East, which are poorly covered by public databases and have high consanguinity rates. We tested and applied a number of quality control (QC) filters. Compared with GWSA, we found that WES provided an accurate prediction of population substructure using variants with a minor allele frequency 〉 2% (correlation = 0.89 with the PCA coordinates obtained by GWSA). WES also yielded highly reliable estimates of homozygosity rates using runs of homozygosity with a 1,000-kb window (correlation = 0.94 with the estimates provided by GWSA). Finally, homozygosity mapping analyses in 15 families including a single offspring with high homozygosity rates showed that WES provided 51% less genome-wide linkage information than GWSA overall but 97% more information for the coding regions. At the genome-wide scale, 76.3% of linked regions were found by both GWSA and WES, 17.7% were found by GWSA only, and 6.0% were found by WES only. For coding regions, the corresponding percentages were 83.5%, 7.4%, and 9.1%, respectively. With appropriate QC filters, WES can be used for PCA and adjustment for population substructure, estimating homozygosity rates in individuals, and powerful linkage analyses, particularly in coding regions.
    Keywords: Exome Sequencing ; Genotyping Array ; Homozygosity Mapping ; Linkage Analysis ; Population Structure ; Consanguinity ; Genetic Linkage ; Genome-Wide Association Study ; Homozygote
    ISSN: 00278424
    E-ISSN: 1091-6490
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  • 3
    Language: English
    In: European Journal of Pediatrics, 2010, Vol.169(9), pp.1069-1074
    Description: Major histocompatibility complex class II plays a key role in the immune response, by presenting processed antigens to CD4+ lymphocytes. Major histocompatibility complex class II expression is controlled at the transcriptional level by at least four trans -acting genes: CIITA, RFXANK , RFX5 and RFXAP . Defects in these regulatory genes cause MHC class II immunodeficiency, which is frequent in North Africa. The aim of this study was to describe the immunological and molecular characteristics of ten unrelated Moroccan patients with MHC class II deficiency. Immunological examinations revealed a lack of expression of MHC class II molecules at the surface of peripheral blood mononuclear cells, low CD4+ T lymphocyte counts and variable serum immunoglobulin (IgG, IgM and IgA) levels. In addition, no MHC class II (HLA DR) expression was observed on lymphoblasts. The molecular analysis identified the same homozygous 752delG26 mutation in the RFXANK genes of all patients. This finding confirms the association between the high frequency of the combined immunodeficiency and the defect in MHC class II expression and provides strong evidence for a founder effect of the 752delG26 mutation in the North African population. These findings should facilitate the establishment of molecular diagnosis and improve genetic counselling for affected Moroccan families.
    Keywords: MHC class II immunodeficiency ; gene ; Molecular diagnosis ; Founder effect ; Morocco
    ISSN: 0340-6199
    E-ISSN: 1432-1076
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  • 4
    Language: English
    In: Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 2014, Vol.369(1645), pp.20130428
    Description: Only a small fraction of individuals exposed to Mycobacterium tuberculosis develop clinical tuberculosis (TB). Over the past century, epidemiological studies have shown that human genetic factors contribute significantly to this interindividual variability, and molecular progress has been made over the past decade for at least two of the three key TB-related phenotypes: (i) a major locus controlling resistance to infection with M. tuberculosis has been identified, and (ii) proof of principle that severe TB of childhood can result from single-gene inborn errors of interferon-γ immunity has been provided; genetic association studies with pulmonary TB in adulthood have met with more limited success. Future genetic studies of these three phenotypes could consider subgroups of subjects defined on the basis of individual (e.g. age at TB onset) or environmental (e.g. pathogen strain) factors. Progress may also be facilitated by further methodological advances in human genetics. Identification of the human genetic variants controlling the various stages and forms of TB is critical for understanding TB pathogenesis. These findings should have major implications for TB control, in the definition of improved prevention strategies, the optimization of vaccines and clinical trials and the development of novel treatments aiming to restore deficient immune responses.
    Keywords: Mendelian Predisposition ; Complex Genetic Predisposition ; Genetic Variant ; Latent Tuberculosis Infection ; Primary Tuberculosis ; Pulmonary Tuberculosis ; Genetic Variation ; Phenotype ; Genetic Predisposition to Disease -- Genetics ; Tuberculosis -- Epidemiology
    ISSN: 09628436
    E-ISSN: 1471-2970
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  • 5
    Language: English
    In: Iranian journal of allergy, asthma, and immunology, 28 August 2013, Vol.12(4), pp.304-11
    Description: The aim of this study was to evaluate the sensitivity of the population of Fez and Casablanca in Morocco to dry white beans (Phaseolus Vulgaris) and to investigate the effect of food processing (heat and/or enzymatic hydrolysis by pepsin) on this sensitivity. Work was based on a bank consisting of 146 sera from patients with atopic hypersensitivity in order to evaluate specific immunoglobulin E (IgE) levels to native and processed white bean proteins by ELISA. Under the same conditions, we assessed the immunoreactivity of rabbit IgG obtained by immunization with native white bean proteins.Evaluation of specific IgE to the white bean proteins showed that 51% of children and 39% of adults had positive values. The heat treatment and pepsin hydrolysis of dry bean proteins showed a reduction of 68% of IgE binding recognition in more than 65% of all patients. After heating, all patients indicated a reduction greater than 36%. With rabbit IgG, we observed a decrease by 25% of binding under heat treatment while enzymatic digestion reduced IgG recognition by 46.6%.These findings suggest that epitopes recognized by IgE in the studied population were conformational sites whereas those recognized by rabbit IgG were probably sequential. In conclusion, these results demonstrate that the Moroccan population was very sensitive to white beans and this sensitivity could be reduced after heat treatment or enzymatic hydrolysis.
    Keywords: Hot Temperature ; Fabaceae -- Immunology ; Food Hypersensitivity -- Immunology ; Immunoglobulin E -- Blood ; Immunoglobulin G -- Blood ; Pepsin A -- Pharmacology
    ISSN: 1735-1502
    E-ISSN: 17355249
    Source: MEDLINE/PubMed (U.S. National Library of Medicine)
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  • 6
    Language: French
    In: Immuno-analyse et biologie spécialisée, June 2012, Vol.27(3), pp.112-116
    Description: The aim is to assess the sensitivity of the population of Fez and Casablanca in Morocco to chicken meat and the effect of heating on this sensitivity. The work is based on a serum bank consisting of 146 sera from patients with suspected allergy. The evaluation of specific IgE to chicken meat showed that 46% of children and 38% of adults have positive values with 12.3% of children and 10% of adults have a higher rate than 20 UI/mL. This high sensitivity shows only partial cross-recognition to the egg whites ranging from 16 to 60%. Heat treatment of meat proteins in chicken showed that more than 80% of these patients show a decrease in the recognition of proteins above 30% after 1 hour at 80 °C. This demonstrates a high thermolability of epitopes recognized by sera from these patients indicating conformational antigenic sites rather than sequential. L’objectif est d’évaluer la sensibilité de la population de Fès et de Casablanca au Maroc à la viande de poulet ainsi que l’effet du chauffage sur cette sensibilité. Le travail repose sur une sérothèque constituée de146 sérums de patients suspects d’allergie. L’évaluation des IgE spécifiques à la viande de poulet a montré que 46 % des enfants et 38 % des adultes ont des valeurs positives dont 12,3 % des enfants et 10 % des adultes ont un taux supérieur à 20 UI/mL. Cette forte sensibilité ne montre qu’une partielle reconnaissance croisée avec le blanc d’œuf variant de 16 à 60 %. Le traitement thermique des protéines de la viande de poulet a montré que plus de 80 % de patients présentent une diminution de la reconnaissance des protéines supérieure à 30 % après une heure à 80 °C. Cela montre une forte thermolabilité des épitopes reconnus par les sérums de ces patients indiquant des sites antigéniques plutôt conformationnels que séquentiels.
    Keywords: Chicken Meat ; Specific Ige ; Heat Treatment ; Allergy to Chicken ; Egg White ; Viande de Poulet ; Ige Spécifique ; Traitement Thermique ; Allergie Au Poulet ; Blanc D’œuf ; Biology
    ISSN: 0923-2532
    E-ISSN: 1878-1365
    Source: ScienceDirect Journals (Elsevier)
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  • 7
    Language: English
    In: Allergologia et Immunopathologia, January 2014, Vol.42(1), pp.29-34
    Description: The aim of this study was to assess the sensitivity profile of the population of Fez and Casablanca in Morocco to dry broad bean ( ), and to investigate the effect of food processing (heat and/or enzymatic hydrolysis by pepsin) on the human IgE binding capacity to broad bean proteins (BBP). Sera samples from 146 patients with atopic hypersensitivity were recruited in order to evaluate specific IgE levels to native and processed broad bean proteins by ELISA. Under the same conditions, we assessed the immunoreactivity of rabbit IgG obtained by immunisation with native BBP. High IgE levels to BBP were found; in fact, 79.3% of children and 80.4% of adults had positive values. The heat treatment (70 °C during 60 min) of dry beans proteins showed slight reduction in recognition of these antigens by rabbit IgG (22%) and by human IgE (12%). Pepsin hydrolysis decreased rabbit-IgG recognition by 55% in the first 30 min of treatment. In contrast, and under the same conditions, pepsin increased human-IgE recognition with an average of 143% for all patients. However, the combination of the two treatments (heating and pepsin digestion) showed a decrease of 16% in BBP recognition for all patients. This study demonstrates a high sensitivity of a Moroccan population to broad bean proteins which was resistant to heat and digestion by pepsin.
    Keywords: Dry Broad Bean ; Enzymatic Hydrolysis ; Food Hypersensitivity ; Heat Treatment ; Specific Ige ; Medicine
    ISSN: 0301-0546
    E-ISSN: 1578-1267
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  • 8
    In: Annals of the New York Academy of Sciences, November 2011, Vol.12381(1), pp.42-52
    Description: The study of inbred populations has contributed remarkably to the description of new autosomal recessive primary immunodeficiencies (PIDs). Here, we examine the pattern of PIDs in North African populations and assess the impact of highly prevalent consanguinity. This review reports on the current status of pediatricians’ awareness of PIDs in Egypt, Morocco, and Tunisia, where awareness of PIDs is relatively recent. The phenotypic distribution of PIDs is reported and compared among the three countries and with other populations. Data analysis reveals a prevalence of autosomal recessive forms and a peculiar distribution of major PID categories, particularly more combined immunodeficiencies than antibody disorders. In these endogamous communities, molecular diagnosis is critical to developing a genetic‐based preventive approach. The organization of diagnosis and care services in these resource‐limited settings faces many obstacles. Autosomal recessive PIDs are overrepresented; thus, it is critical to continue investigation of these diseases in order to better understand the underlying mechanisms and to improve patient care.
    Keywords: Immunodeficiency ; Pediatrics ; Consanguinity ; Recessive ; North Africa
    ISSN: 0077-8923
    E-ISSN: 1749-6632
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  • 9
    Language: English
    In: PLoS ONE, 01 January 2017, Vol.12(5), p.e0176516
    Description: The MYO7A gene encodes a protein belonging to the unconventional myosin super family. Mutations within MYO7A can lead to either non syndromic hearing loss or to the Usher syndrome type 1B (USH1B). Here, we report the results of genetic analyses performed on Moroccan families with autosomal recessive non syndromic hearing loss that identified two families with compound heterozygous MYO7A mutations. Five mutations (c.6025delG, c.6229T〉A, c.3500T〉A, c.5617C〉T and c.4487C〉A) were identified in these families, the latter presenting two differently affected branches. Multiple bioinformatics programs and molecular modelling predicted the pathogenic effect of these mutations. In conclusion, the absence of vestibular and retinal symptom in the affected patients suggests that these families have the isolated non-syndromic hearing loss DFNB2 (nonsyndromic autosomal recessive hearing loss) presentation, instead of USH1B.
    Keywords: Sciences (General)
    E-ISSN: 1932-6203
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  • 10
    In: Chemical Communications, 2018, Vol.54(43), pp.5414-5417
    Description: The mild (electro)chemical oxidation of pyridin-2-ylthio- meso substituted Ni( ii ) porphyrins affords CN fused cationic and dicationic pyridinium-based derivatives. These porphyrins are fully characterized and the molecular structure of one of them was confirmed by X-ray crystallography. A mechanism for the intramolecular oxidative CN coupling is proposed based on theoretical calculations and cyclic voltammetry analyses.
    Keywords: Crystallography ; Oxidation ; Porphyrins ; Coupling (Molecular) ; Substitutes ; Molecular Structure ; Molecular Chains;
    ISSN: 1359-7345
    E-ISSN: 1364-548X
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