Format:
Online-Ressource
ISSN:
1522-2683
Content:
Abstract: A simple procedure is described for counting labeled protein bands after polyacrylamide slab gel electsrophoresis. Proteins labeled with radioisotopes are separated on conventional polyacrylamide slab gels. After fiction and staining, the slab gels are dried onto filter papers under vacuum. The labeled protein patterns are recorded by autoradiography or fluorography. The sample tracks are separated from each other by cutting the dried slab gel lengthwise. Each sample track is cut into predetermined sections by making parallel cuts with a film cutter. The sections are deposited into scintillation vials and digested with 50% hydrogen peroxide. The digested samples are counted in an aqueous scintillator. This procedure provides a direct quantitation of 3h and 14C‐labeled protein bands by reproducibility counting. The results also show that both high resolution and excellent reproducibility are obtainable by this relatively fast and inexpensive method.
In:
volume:2
In:
number:1
In:
year:2005
In:
pages:60-63
In:
extent:4
In:
Electrophoresis, Weinheim : Wiley-Blackwell, 1980-, 2, Heft 1 (2005), 60-63 (gesamt 4), 1522-2683
Language:
English
DOI:
10.1002/elps.1150020110
URN:
urn:nbn:de:101:1-2024040505071426698180
URL:
https://doi.org/10.1002/elps.1150020110
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2024040505071426698180
URL:
https://d-nb.info/1325304859/34
URL:
https://doi.org/10.1002/elps.1150020110
Bookmarklink
