Kooperativer Bibliotheksverbund

Berlin Brandenburg

and
and

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
Type of Medium
Language
Year
  • 1
    Language: English
    In: Analytical and bioanalytical chemistry, November 2010, Vol.398(5), pp.2183-90
    Description: We establish a triple-stacking capillary electrophoresis (CE) separation method to monitor methotrexate (MTX) and its eight metabolites in cerebrospinal fluid (CSF). Three stacking methods with different mechanisms were combined and incorporated into CE separation. Complete stacking and sharp peaks were achieved. Firstly, the optimized buffer (60 mM phosphate containing 15% THF and 100 mM SDS) was filled into the capillary, which was followed by the higher conductivity buffer (100 mM phosphate, 2 psi for 45 s). The analytes extracted from CSF were injected at 2 psi for 99.9 s, which provided long sample zones and pH junction for focusing. Finally, the stacking step was performed by sweeping, and separation was achieved by micellar electrokinetic chromatography. The results of the linear regression equations indicated high linearity (r ≥ 0.9981) over the range of 0.5-7 μM. In intra- and inter-batch results, all data of RSD and RE were below 11%, indicating good precision and accuracy of this method. The LODs (S/N = 3) were 0.1 μM for MTX, 7-hydroxymethotrexate (7-OHMTX) and MTX-polyglutamates (MTX-(Glu)(n, n = 2-5)), 0.2 μM for MTX-(Glu)(6), and 0.3 μM for 2,4-diamino-N(10)-methylpteroic acid (DAMPA) and MTX-(Glu)(7). Our method was implemented for analysis of MTX and its metabolites in the CSF, and could be used for evaluation of its curative effects of acute lymphoblastic leukemia patients. The data were also confirmed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The results showed good coincidence.
    Keywords: Methotrexate ; Electrophoresis, Capillary -- Methods
    ISSN: 16182642
    E-ISSN: 1618-2650
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    In: Journal of Advanced Nursing, July 2014, Vol.70(7), pp.1653-1662
    Description: To purchase or authenticate to the full-text of this article, please visit this link: http://onlinelibrary.wiley.com/doi/10.1111/jan.12328/abstract Byline: Li-Min Wu, Shyh-Shin Chiou, Jiunn-Ming Sheen, Pei-Chin Lin, Yu Mei Liao, Hsing-Mei Chen, Chih-Cheng Hsiao Keywords: coping; intervention; nurses; nursing; paediatric cancer; symptom severity Abstract Aims To evaluate the acceptability and efficacy of a psycho-educational intervention designed to improve effective coping and reduce symptom severity in children with cancer. Background Cancer treatments increase survival rates and also cause physical and psychological effects on children with cancer. A psycho-educational intervention is used to assist children and adolescents with these effects and its efficacy has been described in several studies. Design A randomized controlled trial. Methods Participants being treated were recruited and randomly assigned to two groups from September 2011-February 2013 in Taiwan. The intervention group received a psycho-educational intervention in addition to standard care, while the control group received only standard care. Each participant was assessed using a paediatric cancer coping scale and perceived symptom severity was evaluated at three time points (baseline, 1 month and 3 months). A repeated-measures analysis of variance was used to estimate the effects of intervention. Qualitative findings were analysed using content analysis. Results No significant difference in coping scores was found between groups, but the experimental group reported significantly lower scores in gastrointestinal problems and pain. Most symptoms decreased significantly over time in both groups, except for gastrointestinal problems. The scores in pain, bone marrow suppression and body image showed significant interaction effects between groups on changes over time. Qualitative results reported that participants evaluated the intervention positively, especially about receipt of psychological support and learnt coping skills. Conclusions The psycho-educational intervention administered was acceptable for children with cancer and was found to reduce gastrointestinal problems and pain.
    Keywords: Coping ; Intervention ; Nurses ; Nursing ; Paediatric Cancer ; Symptom Severity
    ISSN: 0309-2402
    E-ISSN: 1365-2648
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 3
    Language: English
    In: Journal of Chromatography A, 2011, Vol.1218(15), pp.2114-2120
    Description: Two single nucleotide polymorphisms (SNPs) of 5,10-methylenetetrahydrofolate reductase ( ) gene, A1298C and C677T, were widely considered to be related with various neoplasia disorders. We established a simple and effective capillary electrophoresis (CE) method for detection of two SNPs in gene simultaneously. DNA samples were amplified by multiplex PCR with universal fluorescence-labeled primer and analyzed by single-strand conformation polymorphism (SSCP)-CE method. The CE method was performed using 1.5% hydroxyethyl cellulose in 1× TBE buffer containing 1 M urea. The PCR products after SSCP procedure were electrokinetically injected at −10 kV, 30 s. Separation voltage was −6 kV and the temperature was set at 20 °C. The optimal SSCP-CE method was applied to detect two polymorphisms in gene of acute lymphoblastic leukemia (ALL) and attention-deficit/hyperactivity disorder (ADHD) patients. Genotyping results were evaluated in terms of relationships between outcomes for ADHD patients after ALL chemotherapy and ALL disease. The SSCP-CE method and multiplex PCR with universal fluorescence primer were used as the fast technique for screening two SNPs in gene, A1298C and C677T. The genotyping data were coincident with DNA sequencing. This SSCP-CE method was found feasible for detecting mutation of gene in populations.
    Keywords: Acute Lymphoblastic Leukemia ; Capillary Electrophoresis ; 5,10-Methylenetetrahydrofolate Reductase ; Genotyping ; Chemistry
    ISSN: 0021-9673
    E-ISSN: 18733778
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 4
    Language: English
    In: Cancer Research, 10/01/2014, Vol.74(19 Supplement), pp.5600-5600
    ISSN: 0008-5472
    E-ISSN: 1538-7445
    Source: CrossRef
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 5
    Language: English
    In: Pediatrics & Neonatology, June 2014, Vol.55(3), pp.159-160
    Keywords: Medicine
    ISSN: 1875-9572
    E-ISSN: 22121692
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 6
    In: ELECTROPHORESIS, July 2016, Vol.37(14), pp.2091-2096
    Description: One CE method was established for detecting deferoxamine (DFO) and deferiprone (DFR) in plasma. For β‐thalassemia patients, DFO and DFR are major medicines to treat the iron overload caused by blood transfusion. Field‐amplified sample injection combined with sweeping was used for sensitivity enhancement in CE. This method was performed on an uncoated fused‐silica capillary. After liquid–liquid extraction, the plasma samples were electrokinetically injected into capillary at +10 kV for 180 s. The phosphate buffer (100 mM) containing 50 mM triethanolamine was used as the BGE (pH 6.6). Separation buffer was phosphate buffer (100 mM, pH 3.0) containing 150 mM SDS. This method showed good linearity ( ≥ 0.9960). Precision and accuracy were evaluated by the results of RSD and relative error of intrabatch and interbatch analyses, and all of the absolute values were less than 6.12%. The LODs (S/N = 3) were 200 ng/mL for DFO, and 25 ng/mL for DFR. The LOQ (S/N = 10) of DFO and DFR were 600 and 75 ng/mL, respectively. This method was applied for clinical applications of five β‐thalassemia patients.
    Keywords: Ce ; Deferiprone ; Deferoxamine ; Stacking ; Β‐Thalassemia
    ISSN: 0173-0835
    E-ISSN: 1522-2683
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 7
    Language: English
    In: Analytical and Bioanalytical Chemistry, 2014, Vol.406(22), pp.5447-5454
    Description: This is the first capillary electrophoresis (CE) analysis for diagnosis of hemophilia A (HA). The intron 22 inversion of factor VIII gene (F8) causes 40–50 % of severe bleeding disorder of HA in all human populations. Consequently, identification of the disease-causing mutations is becoming increasingly important for accurate genetic counseling and prenatal diagnosis. In this study, the key steps of inverse-shifting polymerase chain reaction (IS-PCR) and of short-end injection capillary electrophoresis were used for more specific and rapid genotyping of intron 22 inversion of F8. In IS-PCR, three specific primers were used to amplify 512-bp amplicon for wild type and 584-bp amplicon for patients with intron 22 inversion. The capillary gel electrophoresis (CGE) system was performed using 1× Tris–borate–EDTA (TBE) buffer containing 0.3 % ( w / v ) polyethylene oxide (PEO). The PCR amplicons were electrokinetically injected at 10 kV for 10 s at a temperature of 25 °C. The optimal short-end injection CGE was applied to detect the F8 gene of HA patients and carriers within 5 min. Intron 22 inversion was indeed found on some HA patients (13/35, 37.1 %). All genotyping results showed good agreement with DNA sequencing method and long-distance polymerase chain reaction (LD-PCR). The IS-PCR combined with short-end injection CGE method was feasible and efficient for intron 22 inversion screening of F8 in the HA populations.
    Keywords: Factor VIII gene ; Hemophilia A ; Intron 22 inversion ; Capillary electrophoresis ; Inverse-shifting PCR
    ISSN: 1618-2642
    E-ISSN: 1618-2650
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 8
    Language: English
    In: ELECTROPHORESIS, August 2011, Vol.32(15), pp.2021-2027
    Description: The γ‐glutamyl hydrolase () gene plays an important role in methotrexate (MTX) metabolism, ensuring that MTX polyglutamates (MTX‐(Glu)) could be converted back into MTX. Accumulation of MTX‐(Glu) is a problem in MTX therapy. SNP 452 C〉T has been reported to associate with lower catalytic activity and higher accumulation of long‐chain MTX‐(Glu) in patients treated with higher doses of MTX treatment. We propose and establish a simple and effective CE method for detecting SNP in gene. The DNA samples after amplification were analyzed by SSCP‐CE method. The CE conditions were generated by using 1× TBE buffer containing 1.5% w/v hydroxypropyl methyl cellulose under reverse polarity at 25°C. This method was applied to detect genotyping of acute lymphoblastic leukemia patients receiving MTX treatment. The results were confirmed by DNA sequencing with good agreement. Concentrations of MTX‐(Glu) in whole blood were analyzed by on‐line stacking CE method. MTX‐(Glu) levels and genotypes in gene of acute lymphoblastic leukemia patients were evaluated. The SSCP‐CE method was found to be feasible for SNP screening in the gene.
    Keywords: Γ‐Glutamyl Hydrolase Gene ; Snp ; Sscp‐Ce
    ISSN: 0173-0835
    E-ISSN: 1522-2683
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 9
    Language: English
    In: Supportive Care in Cancer, 2010, Vol.18(12), pp.1581-1587
    Description: Byline: Shyh-Shin Chiou (1), Ren-Chin Jang (1), Yu-Mei Liao (1), Pinchen Yang (2,3) Keywords: Cancer survivor; Health-related quality of life; Pediatric leukemia; Adolescent Abstract: Purpose Long-term survival of childhood leukemia has become a reality with treatment advancement hence, the need to assess the survivors' health-related quality of life (HRQL) is essential. Although a growing number of Western studies have documented the considerable impact of diagnosis and treatment on HRQL in pediatric leukemia survivors, little finding has been reported in non-Western developing countries. Methods We used a previously validated 14-dimensional questionnaire, Child Health Questionnaire 50-item Parent Form (CHQ-PF 50), to examine the perceived HRQL of 32 child/adolescent survivors, currently aged 13.17+-2.49 years, who had experienced first complete continuous remission from leukemia for at least 3 years. The HRQL status was compared with that obtained from community subjects (N=154) and survivors' nonadult siblings (N=30). Intelligence quotients (IQ) and computerized neuropsychological assessments were performed for subjects. Results The HRQL of leukemia survivors was noted to be worse than that of community children and nonadult siblings as reflected by significantly lower scores in both the physical summary and the psychosocial summary score of CHQ-PF 50. 15.6% of the survivors had impaired intelligence (estimated IQ below 70). 27.8% of the adolescents were impaired in the cognitive domains as assessed by neuropsychological tests. Conclusions In this Taiwanese single institution experience, pediatric leukemia survivors carried a morbidity burden into their teen years as reflected by worse HRQL than controls. These findings may guide the support required by this population. Author Affiliation: (1) Department of Pediatrics, College of Medicine, Kaohsiung Medical University and Kaohsiung Medical University Hospital, Kaohsiung, Taiwan (2) Department of Psychiatry, College of Medicine, Kaohsiung Medical University and Kaohsiung Medical University Hospital, Kaohsiung, Taiwan (3) Department of Psychiatry, Kaohsiung Medical University Hospital, 100, Shin-Chuan 1st Road, Kaohsiung, 807, Taiwan Article History: Registration Date: 09/11/2009 Received Date: 05/05/2009 Accepted Date: 09/11/2009 Online Date: 01/12/2009
    Keywords: Cancer survivor ; Health-related quality of life ; Pediatric leukemia ; Adolescent
    ISSN: 0941-4355
    E-ISSN: 1433-7339
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 10
    Language: English
    In: Cancer research, 01 August 2017, Vol.77(15), pp.4065-4077
    Description: The intestine-specific homeobox transcription factor intestine-specific homeobox (ISX) is an IL6-inducible proto-oncogene implicated in the development of hepatocellular carcinoma, but its mechanistic contributions to this process are undefined. In this study, we provide evidence that ISX mediates a positive feedback loop integrating inflammation, tryptophan catabolism, and immune suppression. We found that ISX-mediated IL6-induced expression of the tryptophan catabolic enzymes Indoleamine 2,3-dioxygenase 1 (IDO1) and tryptophan 2,3-dioxygenase in hepatocellular carcinoma cells, resulting in an ISX-dependent increase in the tryptophan catabolite kynurenine and its receptor aryl hydrocarbon receptor (AHR). Activation of this kynurenine/AHR signaling axis acted through a positive feedback mechanism to increase ISX expression and enhance cellular proliferation and tumorigenic potential. RNAi-mediated attenuation of ISX or AHR reversed these effects. In an IDO1-dependent manner, ectopic expression of ISX induced expression of genes encoding the critical immune modulators CD86 (B7-2) and programmed death ligand-1 (PD-L1), through which ISX conferred a significant suppressive effect on the CD8 T-cell response. In hepatocellular carcinoma specimens, expression of IDO1, kynurenine, AHR, and PD-L1 correlated negatively with survival. Overall, our results identified a feed-forward mechanism of immune suppression in hepatocellular carcinoma organized by ISX, which involves kynurenine-AHR signaling and PD-L1, offering insights into immune escape by hepatocellular carcinoma, which may improve its therapeutic management. .
    Keywords: Carcinoma, Hepatocellular -- Metabolism ; Homeodomain Proteins -- Metabolism ; Liver Neoplasms -- Metabolism ; Transcription Factors -- Metabolism ; Tryptophan -- Metabolism ; Tumor Escape -- Physiology
    ISSN: 00085472
    E-ISSN: 1538-7445
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. Further information can be found on the KOBV privacy pages