Bioorganic & Medicinal Chemistry, 01 July 2013, Vol.21(13), pp.3866-3872
The addition of a DNA methyltransferase inhibitor, 5-azacytidine, to fungus culture broth changed its secondary metabolites profile. Analysis of the culture broth extract led to the isolation of three new bisabolane-type sesquiterpenoids: (7 )-(+)-7- -methylsydonol ( ), (7 ,11 )-(+)-12-hydroxysydonic acid ( ) and 7-deoxy-7,14-didehydrosydonol ( ), along with eight known compounds. The isolated compounds were evaluated for their anti-diabetic and anti-inflammatory activities. Among the isolates, ( )-(+)-sydonol ( ) did not only potentiate insulin-stimulated glucose consumption but also prevented lipid accumulation in 3T3-L1 adipocytes. Additionally, ( )-(+)-sydonol ( ) exhibited significant anti-inflammatory activity through inhibiting superoxide anion generation and elastase release by fMLP/CB-induced human neutrophils. This is the first report on isolating a secondary metabolite with anti-diabetic and anti-inflammatory activities from microorganisms.
Epigenetic ; Aspergillus Sydowii ; Bisabolane-Type ; Anti-Diabetic ; Anti-Inflammatory ; Medicine ; Chemistry ; Anatomy & Physiology
View record in ScienceDirect (Access to full text may be restricted)