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  • 1
    In: PLoS ONE, 2015, Vol.10(10)
    Description: In this study, six 2-phenylnaphthalenes with hydroxyl groups were synthesized in high yields by the demethylation of the corresponding methoxy-2-phenylnaphthalenes, and one 2-phenylnaphthalene with an amino group was obtained by hydrogenation. All of the 2-phenylnaphthalene derivatives were evaluated for cytotoxicity, and the structure-activity relationship (SAR) against human breast cancer (MCF-7) cells was also determined. The SAR results revealed that cytotoxicity was markedly promoted by the hydroxyl group at the C-7 position of the naphthalene ring. The introduction of hydroxyl groups at the C-6 position of the naphthalene ring and the C-4 ' position of the phenyl ring fairly enhanced cytotoxicity, but the introduction of a hydroxyl group at the C-3 ' position of the phenyl ring slightly decreased cytotoxicity. Overall, 6,7-dihydroxy-2-(4'-hydroxyphenyl)naphthalene ( PNAP - 6h ) exhibited the best cytotoxicity, with an IC 50 value of 4.8 μM against the MCF-7 cell line, and showed low toxicity toward normal human mammary epithelial cells (MCF-10A). PNAP - 6h led to cell arrest at the S phase, most likely due to increasing levels of p21 and p27 and decreasing levels of cyclin D1, CDK4, cyclin E, and CDK2. In addition, PNAP - 6h decreased CDK1 and cyclin B1 expression, most likely leading to G 2 /M arrest, and induced morphological changes, such as nuclear shrinkage, nuclear fragmentation, and nuclear hypercondensation, as observed by Hoechst 33342 staining. PNAP - 6h induced apoptosis, most likely by the promotion of Fas expression, increased PARP activity, caspase-7, caspase-8, and caspase-9 expression, the Bax/Bcl-2 ratio, and the phosphorylation of p38, and decreased the phosphorylation of ERK. This study provides the first demonstration of the cytotoxicity of PNAPs against MCF-7 cells and elucidates the mechanism underlying PNAP-induced cytotoxicity.
    Keywords: Research Article
    E-ISSN: 1932-6203
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  • 2
    In: 中国癌症研究:英文版 - Chinese Journal of Cancer Research, 2012, Vol.24(02), pp.109-115
    Description: Objective: To examine the apoptotic effect of ent-llα-hydroxy-15-oxo-kaur-16-en-19-oic-acid (5F), a compound isolated from Pteris semipinnata L (PsL), in human lung cancer A549 cells. Methods: A549 cells were treated with 5F (0-80 lag/ml) for different time periods. Cytotoxicity was examined using a Ml-I- method. Cell cycle was examined using propidium iodide staining. Apoptosis was examined using Hoechst 33258 staining, enzyme-linked immunosorbent assay (ELISA) and caspase-3 activity analysis. Expression of representative apoptosis-related proteins was evaluated by Western blot analysis. Reactive oxygen species (ROS) level was measured using standard protocols. Potential interaction of 5F with cisplatin was also examined. Results: 5F inhibited the proliferation of A549 cells in a concentration- and time-dependent manner. 5F increased the accumulation of cells in sub-G1 phase and arrested the cells in the G2 phase. Exposure to 5F induced morphological changes and DNA fragmentation that are characteristic of apoptosis. The expression of p21 was increased. 5F exposure also increased Bax expression, release of cytochrome c and apoptosis inducing factor (AIF), and activation of caspase-3. 5F significantly sensitized the cells to cisplatin toxicity. Interestingly, treatment with 5F did not increase ROS, but reduced ROS production induced by cisplatin. Conclusion: 5F could inhibit the proliferation of A549 cells by arresting the cells in G2 phase and by inducing mitochondrial-mediated apoptosis.
    Keywords: A549细胞 ; 细胞凋亡 ; 细胞周期 ; Caspase ; 3 ; 氧代 ; 羟基 ; 肺癌 ; 凋亡诱导因子 ; Pteris Semipinnata L; Lung Cancer; G2 Cell Cycle Arrest; Apoptosis; Reactive Oxygen Species
    ISSN: 1000-9604
    Source: 维普数据 (Chongqing VIP Information Co.)
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  • 3
    In: 环境与健康杂志 - Journal of Environment and Health, 2017, Vol.34(06), pp.516-520
    Description: 目的探讨颈动脉内中膜厚度增厚与否对血压与颈动脉内中膜厚度相关性的影响。方法研究人群来自北京大学第一医院心内科于2011年12月至2012年4月在北京市石景山区横断面调查的动脉粥样硬化研究队列。纳入标准为问卷调查资料完整并完成颈动脉超声图像的采集且未应用过任何降压药物,研究人群(n=2 569)以最大颈动脉内中膜厚度0.9 mm为分界点分为颈动脉内中膜厚度增厚组(n=1 597)及非增厚组(n=972),分析血压与颈动脉内中膜厚度的关系。结果多重线性回归分析显示,在调整了性别、年龄、BMI、目前吸烟饮酒状况、血肌酐、空腹血糖、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、甘油三酯、降糖降脂药物使用、心血管疾病患病情况等因素后,总体人群各项血压指标(收缩压、舒张压、脉压及平均动脉压)均与颈动脉内中膜厚度平均值呈显著正相关(收缩压:β=0.009,P〈0.000 1;舒张压:β=0.002,P〈0.000 1;脉压:β=0.007,P〈0.000 1;平均动脉压:β=0.009,P〈0.000 1);在颈动脉内中膜厚度≥0.9 mm组各项血压指标依然与颈动脉内中膜厚度平均值呈显著正相关(收缩压:β=0.006,P=0.000 1;舒张压:β=0.001,P=0.000 1;脉压:β=0.005,P〈0.0001;平均动脉压:β=0.005,P=0.023 8),而非增厚组中各项血压指标与颈动脉内中膜厚度平均值均无明显相关。结论本研究发现在颈动脉内中膜厚度增厚的人群中血压指标与颈动脉内中膜厚度显著相关,非增厚人群中无显著相关。提示血管壁增厚影响血压的作用机制在二者相关性机制方面可能占据更重要的地位。
    Description: Objective The aim of this study was to investigate the influence of the thickening of carotid intima-media thickness(cIMT) on the association between peripheral blood pressure and carotid intima-media thickness in a Chinese community-based population. Methods From December 2011 to April 2012, a total of 2 569 subjects with normal blood pressure or with hypertension but not receiving antihypertensive drugs were enrolled in this study from an atherosclerosis cohort in Shijingshan district, Beijing. Subjects were divided into two groups according to whether their maximum thickness of cIMT was greater than0.9 mm. The association between blood pressure and cIMT was evaluated in the whole population, and in the population whose cIMT 0.9 mm, and in those whose cIMT≥0.9 mm, respectively. Results In the whole population, systolic blood pressure(SBP,β=0.009,P〈0.000 1), diastolic blood pressure(DBP, β=0.002,P〈0.000 1), pulse pressure(PP, β=0.007,P〈0.000 1) and mean arterial pressure(MAP, β=0.009,P〈0.000 1) were positively correlated with the average of cIMT respectively after adjusting for sex, age, BMI, current smoking and drinking, serum creatinine, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, total triglycerides, fasting blood glucose, cardiovascular disease history, antidiabetic medications and hypolipidemic medications. In the subjects with maximum cIMT ≥0.9 mm, the association between blood pressure and cIMT remained significant(SBP: β =0.009,P =0.000 1; DBP: β =0.001,P =0.000 1; PP: β =0.009, P 0.000 1; MAP: β =0.005, P =0.023 8).Conclusion The study showed that blood pressurewas correlated with cIMT only in the subjects with thickening cIMT.It is suggested that the influence of the artery thickening on peripheral blood pressure may have a major position for the association between them.
    Keywords: 颈动脉内中膜厚度 ; 收缩压 ; 舒张压 ; 脉压 ; 平均动脉压 ; Carotid Intima-Media Thickness; Systolic Blood Pressure; Diastolic Blood Pressure; Pulse Pressure; Mean Arterial Pressure;
    ISSN: 1001-5914
    Source: 维普数据 (Chongqing VIP Information Co.)
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  • 4
    Description: 碩士 國立臺灣大學 微生物學研究所 107 Protein aggregation can be found in a variety of diseases such as Parkinson’s and Alzheimer’s diseases. Many stresses at the molecular and cellular levels have been identified to provoke aging and play an important role in neurodegenerative diseases. Upon these stresses induce DNA damage response, ATM...
    Source: Networked Digital Library of Theses and Dissertations
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  • 5
    In: 中国血管外科杂志(电子版) - Chinese Journal of Vascular Surgery(Electronic Version), 2014, Issue 01, pp.38-40
    Description: 目的探讨去股浅动脉交感神经化治疗血栓闭塞性脉管炎(TAO)的效果。方法回顾分析本院自2013年1月至2013年8月间3例用常规动脉腔内球囊扩张术的同时施行血管腔内射频消融术的TAO病例,通过踝肱指数(ABI)、血管通畅程度等评判临床疗效。结果 3例患者手术均成功,术后临床症状均有明显改善,ABI从术前的0.3、0.2和0.3上升至术后1.0、0.8和0.8。患者在随访期间临床症状保持良好,均无复发,CT血管造影显示动脉血流通畅。结论利用血管腔内技术射频消融去股浅动脉交感神经化治疗TAO,既可达到腰交感神经节切除的治疗效果,又可避免并发症的发生。但因例数较少,随访时间较短,其中远期效果仍有待于进一步的研究。
    Keywords: 血栓闭塞性脉管炎 ; 射频消融术 ; 去神经化 ; 股浅动脉
    ISSN: 1674-7429
    Source: 维普数据 (Chongqing VIP Information Co.)
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  • 6
    Language: English
    In: Acta pharmacologica Sinica, July 2002, Vol.23(7), pp.597-600
    Description: To investigate the mechanism of xiaoyu tablet on reduction of smooth muscle cells (SMC) in atherosclerotic vessel wall. The atherosclerotic model was performed in male New Zealand rabbits that were given high fat diet and abrasion of the abdominal aorta endothelial cells. The rabbits were then administered with xiaoyu tablet 0.16-0.32 g x kg(-1) x d(-1) for 16 weeks. Changes in morphology, endothelin (ET)-1, nitric oxide (NO), and apoptotic cells of atherosclerotic vessel wall were determined by the microscopy, radioimmunoassay, colorimetric method, the techniques of DNA in situ end labeling, and image pattern analysis, respectively. After 16 weeks of xiaoyu tablet treatment, intimal thickness and SMC in atherosclerotic vessel wall were diminished, ET-1 was decreased by 8.2 %-42.6 %, NO was increased by 7.5 %-54.2 %, and labeled apoptotic nuclei were markedly decreased, the area and integral optical density of positive granule were (846+/-308) microm2 and 3425+/-1374 in atherosclerotic group and (225+/-60) microm2 and 1445+/-606 in xiaoyu tablet 0.32 g/kg group, respectively. Xiaoyu tablet not only inhibited proliferation of SMC through reducing ET-1 in atherosclerotic vessel wall, but also induced apoptosis of SMC by increasing NO in vessel wall.
    Keywords: Apoptosis -- Drug Effects ; Arteriosclerosis -- Pathology ; Drugs, Chinese Herbal -- Pharmacology ; Endothelin-1 -- Metabolism ; Nitric Oxide -- Metabolism
    ISSN: 1671-4083
    E-ISSN: 17454083
    Source: MEDLINE/PubMed (U.S. National Library of Medicine)
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  • 7
    In: 现代预防医学 - Modern Preventive Medicine, 2012, Vol.39(11), pp.2806-2808
    Description: 目的研究围生期暴露于双酚A对SD大鼠子代的行为发育的影响。方法 120只孕鼠随机分为6组,每组20只。从孕期d 6至仔鼠出生d 21断乳每天分别灌胃给予0、0.05、0.5、5、50、500mg/kg的双酚A。于仔鼠出生后d 3检测前、后肢定位反射;出生后4 d检测仔鼠的平面翻正、悬崖回避反射;出生后d 5检测负向地性;出生后d 11检测空中翻正反射。结果 0.05、0.5mg/kg剂量组的所有的神经反射均与对照组无明显差异,5mg/kg剂量组的平面翻正反射、负向地性反射、空中翻正反射达标天数与对照组相比显著延迟;50mg/kg剂量组,在前、后肢定位反射、空中翻正反射达标天数与对照组相比显著延迟;500mg/kg剂量组,在前、后肢定位反射、负向地性反射、空中翻正反射以及悬崖回避反射的达标天数与对照组相比显著延迟。结论大鼠围生期暴露于双酚A,可影响子代大鼠的神经行为发育。
    Description: OBJECTIVE To explore the effect of perinatal exposure to Bisphenol A on behavioral development of the offspring of rats.METHODS 120 pregnant Sprague-Dawley rats were randomly divided into 6 groups.Each group had 20 rats.Rats were received by gavage 0,0.05,0.5,5,50 or 500mg/kd/day of bisphenol A from gestation day(G)6 to postnatal day(PND)21.Forelimb reflex and rear limb reflex were tested on offspring from PND3.Surface righting reflex and cliff avoidance reflex were tested on offspring from PND4.Negative geotaxis reflex were tested on offspring from PND5.Air righting reflex was tested on offspring from PND11.RESULTS Delay in forelimb reflex,rear limb reflex of offspring were observed in 50mg/kg and 500mg/kg groups.Retardation in surface righting reflex of offspring was observed in 5mg/kg group.Delay in cliff avoidance reflex of offspring was observed in 500mg/kg group.Delay in negative geotaxis reflex of offspring was observed in 5mg/kg and 500mg/kg groups.Delay in air righting reflex of offspring was observed in 5mg/kg,50mg/kg and 500mg/kg groups.CONCLUSION Perinatal exposure to bisphenol A could affect the behavioral development of rat offspring.
    Keywords: 围生期 ; 双酚a ; 大鼠子代 ; 行为发育 ; Bisphenol A; Perinatal Exposure; Rats; Behavioral Development
    ISSN: 1003-8507
    Source: 维普数据 (Chongqing VIP Information Co.)
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  • 8
    Book
    Book
    Shenyang Shi: Liaoning ke xue ji shu chu ban she
    Language: Chinese
    Description: 20, 1678 p.
    Description: Includes indexes
    Keywords: Chinese Language--Dictionaries--Japanese ; Chinese Language--English--Dictionaries ; Medicine--Dictionaries--Chinese
    ISBN: 7538119590
    ISBN: 9787538119596
    Source: HathiTrust
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  • 9
    In: 时珍国医国药 - Lishizhen Medicine and Materia Medica Research, 2007, Vol.18(1), pp.102-103
    Description: 目的建立草药半边旗中木犀草素含量的准确测定方法,并测定不同采收期半边旗中木犀草素的含量。方法采用高效液相色谱法,色谱柱为Diamonsil C18流动相为甲醇水梯度洗脱,流速0.2ml·min^-1,检测波长为283nm,柱温度35℃。结果木犀草素进样量在0.062-0.62μg范围内线性关系良好(R^2=0.9987),平均回收率为99.5%(RSD=1.98%)。结论该方法操作简便,结论可靠,可用于半边旗草药的质量控制。
    Description: Objective To establish an HPLC method for content determination of luteolin from PSL. Methods The separation was performed on a Diamonsil C18 column, the mobile phase was run with a mixture of methanol(B) and water(A) with a linear gradient elution at a flow rate of 0.2 ml · min , the ultraviolet wavelength...
    Keywords: 高效液相色谱 ; 半边旗 ; 黄酮 ; 木犀草素 ; Hplc; Pteris Semipinnata; Flavonoid; Luteolin
    ISSN: 1008-0805
    Source: 维普数据 (Chongqing VIP Information Co.)
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  • 10
    Language: Chinese
    In: 上海中醫藥雜誌 / Shanghai Journal of Traditional Chinese Medicine, 2008-10-10, 42卷10期 (Vol.42, Issue 10), pp.48-50
    Description: Objective To observe the therapeutic effects of electroacupuncture for chronic fatigue syndrome (CFS). Methods Sixty CFS patients were randomly divided into electroacupuncture acupoint group and electroacupuncture non-acupoint group, and respectively treated by Shenshu (BL 23) and Zusanli (ST 36) or by non-acupoints. The severity of fatigue, potential symptoms, pain severity and the quality of life were evaluated. Results The electroacupuncture at acupoints Shenshu (BL23) and Zusanli (ST36) decreased the integral of FSS, SPHERE, VAS and enhanced the integral of SF-36 (P<0.01). Compared with control group, there was considerable differences (P<0.01). Conclusion Electroacupuncture at Shenshu (BL 23) and Zusanli (ST 36) can ease the clinical symptoms and improve the quality of life of CFS.
    Keywords: 慢性疲劳综合征 ; 电针 ; 肾俞 ; 足三里 ; 随机对照试验 ; Chronic Fatigue Syndrome ; Electroacupuncture ; Shenshu Bl 23 ; Zusanli St 36 ; Randomized Controlled Trial
    ISSN: 1007-1334
    Source: 中文電子期刊服務 - Chinese Electronic Periodical Services (CEPS) (Airiti Library)〈img src="http://exlibris-pub.s3.amazonaws.com/airiti_logo.gif" style="vertical-align:middle;margin-left:7px"〉
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