Format:
Online-Ressource (XI, 138p. 35 illus., 6 illus. in color, digital)
ISBN:
9781603272193
,
9781283445726
Series Statement:
Infectious Disease
Content:
David S. Wunschel
Content:
Combining the disciplines of biological, physical and chemical science, microbial forensics has a rapidly rising profile in a world increasingly troubled by the threat of 'biocrime' and 'bioterrorism'. This valuable resource is a major addition to a body of literature reckoned to lack sufficient breadth. It presents a variety of phenotypic and trace signature methodologies associated with cultured microorganisms that, despite being genetically identical, may be characterized by differing cultural environments. One of the central challenges faced by those working in this field is the sheer dive
Note:
Includes bibliographical references and index
,
Chemical and Physical Signatures for Microbial Forensics; Foreword; Preface; Contents; Contributors; 1: Statistical Foundations and Data Integration for Microbial Forensics; 1.1 The Role of Statistics in Microbial Forensics; 1.1.1 Daubert V. Merrell Dow Pharmaceuticals; 1.1.1.1 SWGMGF; 1.1.2 Experimental Planning; 1.1.2.1 Conducting Proof-of-Concept Studies; 1.1.2.2 Establishing Standard Operating Protocols and Quality Control Procedures; 1.1.2.3 Performing Final Validation Studies; 1.1.3 Statistical Methods for Characterizing Unknown Samples
,
1.1.3.1 Feature Extraction and Dimension Reduction1.1.3.2 Comparing Unknown Samples to Known Samples; 1.1.3.3 Comparing Unknown Samples to a Database of Reference Signatures; Constructing a Reference Database; Matching an Unknown to a Database Signature; 1.1.4 Integrated Microbial Forensics; 1.1.4.1 Combining Multiple Signatures; 1.1.4.2 Example: A Bayesian Network for Integrated Microbial Forensics; 1.1.5 Summary; References; 2: The Microbe: The Basics of Structure, Morphology, and Physiology as They Relate to Microbial Characterization and Attribution; 2.1 Introduction
,
2.2 How Microbiologists Look at Microbes2.2.1 Classical Methods Applied to Forensic Characterization of Unknown Samples; 2.2.2 Culture Conditions as a Consideration in Forensic Microbiology; 2.2.3 Viable but Not Culturable as a Microbial Life Stage; 2.2.4 Impact of Culture on Microbial Identification and Attribution Elements; 2.3 Terminology Important to Distinguishing Microbes; 2.3.1 Microbial Speciation Is a Continuum; 2.3.2 Adopted Information as a Consideration for Attribution; 2.4 Nucleic Analysis for Microbial Characterization: The New Gold Standard
,
2.4.1 Polymerase Chain Reaction (PCR): An Enabling Technology for Microbial Genomics2.4.2 Ribosomal RNA for Microbial Identification of Species and Strain; 2.4.3 Repetitive Element Pattern Analysis for Bacterial Identification; 2.4.4 Whole Genome Analysis: Canonical Sequences and SNP Analysis; 2.5 Proteome and Intent as Determined by Mass Spectrometry and Other Multitarget Approaches; 2.6 Conclusion: A Toolbox for Characterization of Microbes for Forensics; References; 3: Fatty Acids and Lipids; 3.1 Introduction; 3.1.1 Lipids and Their Role in Microbes; 3.2 Lipid Variation in Microbes
,
3.2.1 Fatty Acids3.2.1.1 Factors Affecting Fatty Acid Composition: Taxonomy and Cell Type; 3.2.1.2 Growth and Culturing Conditions; 3.2.1.3 Methods for Extracting Fatty Acids from Whole Cells; Chemical Extraction of Fatty Acids; In Situ Fatty Acid Extraction; Phospholipid Fatty Acid (PLFA) Extraction; 3.2.1.4 Methods for Separation and Detection of Fatty Acid Derivatives; Gas Chromatography; Mass Spectrometry; High-Performance Liquid Chromatography (HPLC); Infrared Spectroscopy; Isotope Ratio Mass Spectrometry (IRMS); 3.2.2 Lipopolysaccharides (LPS); LPS Variation: Lipid A; 3.2.2.2 O Antigen
,
3.2.2.3 Methods for LPS Extraction
Additional Edition:
ISBN 9781603272179
Additional Edition:
Buchausg. u.d.T. Chemical and physical signatures for microbial forensics New York, NY : Springer, 2012 ISBN 9781603272179
Language:
English
Keywords:
Mikrobiologie
;
Forensik
;
Mikroorganismus
;
Mikrobiologie
;
Forensik
;
Mikroorganismus
DOI:
10.1007/978-1-60327-219-3
URL:
Volltext
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