UID:
almahu_9949251276102882
Format:
1 online resource (430 p.)
Edition:
1st ed. 2002.
ISBN:
1-280-83068-9
,
1-59259-284-8
,
9786610830688
Series Statement:
Methods in Molecular Biology, 197
Content:
Mutations within mitochondrial DNA (mtDNA) and the nuclear genes involved in the maintenance of mitochondrial DNA have been linked to a wide range of human diseases, including several of the most common diseases of aging. In Mitochondrial DNA: Methods and Protocols internationally recognized authorities describe in great detail the methods they have perfected to analyze mtDNA and the proteins involved in its maintenance. The analytical techniques cover the purification of mtDNA from a variety of sources and the analysis of DNA for both deletions, point mutations, and damage, for replication intermediates, and for following the fate of mtDNA outside of the mitochondria. Additional analytical methods are presented for analyzing the proteins and enzymes that maintain mtDNA. Each readily reproducible protocol includes step-by-step instructions, tips on avoiding pitfalls and extending the method to other situation, and introductory material explaining the theory behind the process. Comprehensive and timely, Mitochondrial DNA: Methods and Protocols offers both basic and clinical researchers proven cutting-edge methods for analyzing the role mtDNA plays in the aging process, apoptosis, and possibly some cancers, and for investigating the cause of mitochondrial dysfunction and disease.
Note:
Description based upon print version of record.
,
Methods for the Analysis of MTDNA -- Animal Models for Mitochondrial Disease -- Identification of Mutations in mtDNA from Patients Suffering Mitochondrial Diseases -- Screening for Aging-Dependent Point Mutations in mtDNA -- Scanning Low-Frequency Point Mutants in the Mitochondrial Genome Using Constant Denaturant Capillary Electrophoresis -- Identification of mtDNA Mutations in Human Cancer -- In Situ PCR of the Common Human mtDNA Deletion -- Reproducible Quantitative PCR of Mitochondrial and Nuclear DNA Copy Number Using the LightCycler™ -- Yeast Nuclear Genes for mtDNA Maintenance -- Measuring mtDNA Mutation Rates in Saccharomyces cerevisiae Using the mtArg8 Assay -- Measuring Oxidative mtDNA Damage and Repair Using Quantitative PCR -- Migration of mtDNA into the Nucleus -- 2D Gel Electrophoresis of mtDNA -- Methods for the Analysis of proteins involved in MTDNA Transactions -- Purification of Mitochondria for Enzymes Involved in Nucleic Acid Transactions -- Purification of Mitochondrial Uracil-DNA Glycosylase Using Ugi-Sepharose Affinity Chromatography -- Characterization of Specialized mtDNA Glycosylases -- Purification, Separation, and Identification of the Human mtDNA Polymerase With and Without Its Accessory Subunit -- Assay of mtDNA Polymerase ? from Human Tissues -- Purification Strategies for Drosophila mtDNA Replication Proteins in Native and Recombinant Form -- Purification Strategies for Drosophila mtDNA Replication Proteins in Native and Recombinant Form -- Expression, Purification, and In Vitro Assays of Mitochondrial Single-Stranded DNA-Binding Protein -- Recombinant Yeast mtDNA Helicases Purification and Functional Assays -- Isolation of DNA Topoisomerases I and II Activities from Mitochondria of Mammalian Heart -- 23 Endonuclease G Isolation and Assays -- 24 Targeting DNA Repair Proteins to Mitochondria -- 25 In Organello Footprinting of mtDNA -- Crosslinking of Proteins to mtDNA -- Breeding and Genotyping of Tfam Conditional Knockout Mice.
,
English
Additional Edition:
ISBN 0-89603-972-2
Additional Edition:
ISBN 0-585-40538-7
Language:
English
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