Kooperativer Bibliotheksverbund

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Format: XVI, 339 S. : Ill., graph. Darst.

ISBN: 0-12-604040-0 , 0-12-564146-X

Series Statement: Methods in cell biology 45

Language: English

Subjects: Biology

Keywords: Pathogene Bakterien ; Pathogenese ; Cytologie ; Pathogene Bakterien ; Eukaryontische Zelle ; Molekularbiologie

URL: http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006749095&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA

URL: Inhaltsverzeichnis

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Format: 1 online resource.

ISBN: 9781683671336 , 1683671333

Additional Edition: Print version: Phagocyte-pathogen interactions. Washington, D.C. : ASM, 2009 ISBN 9781555814014

Language: English

Keywords: Electronic books. ; Electronic books. ; Electronic books.

URL: https://onlinelibrary.wiley.com/doi/book/10.1128/9781555816650

URL: https://onlinelibrary.wiley.com/doi/book/10.1128/9781555816650

URL: https://onlinelibrary.wiley.com/doi/book/10.1128/9781555816650

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Format: xvi, 339 p. : , ill. (some col.).

Edition: Electronic reproduction. Ann Arbor, MI : ProQuest, 2016. Available via World Wide Web. Access may be limited to ProQuest affiliated libraries.

Series Statement: Methods in cell biology ; v. 45

Language: English

Keywords: Electronic books.

URL: Click to View

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Format: XV, 559 S. : , Ill., graph. Darst. ; , 29 cm.

ISBN: 1-555-81401-8 , 978-1-555-81401-4

Note: Literaturangaben

Language: English

Subjects: Biology

Keywords: Phagozyt ; Makrophage ; Parasit ; Wirt

URL: http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020413030&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA

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Format: 1 online resource (363 p.)

ISBN: 1-281-75562-1 , 9786611755621 , 0-08-085943-7

Series Statement: Methods in cell biology ; v. 45

Content: Microbes as Tools for Cell Biology bridges the gap between cell biology and microbiology. This laboratory guide provides a microbial tool kit for biologists who wish to use microbes as probes for basic cellular functions. The volume is organized into three sections, covering essential information on culture and genetic manipulation of microbes, assays for pathogen-host recognition, and analysis of intracellular parasitism. Each chapter outlines practical procedures and describes the rationale behind their development. This volume should prove useful to anyone interested in the biology o

Note: Description based upon print version of record. , Front Cover; Microbes as Tools for Cell Biology; Copyright Page; Contents; Contributors; Preface; Introduction; Chapter 1. Obtaining and Maintaining Microbial Pathogens; I. Introduction; II. Biosafety Considerations; III. Department of Health and Human Services Guidelines; IV. Federal and Institutional Requirements; V. Source of Pathogens; VI. Culture and Maintenance of Pathogens; VII. Conclusion; Part I: Manipulation of Pathogens; Chapter 2. Cultivation of Malaria Parasites; I. Introduction; II. Culture Medium; III. Serum; IV. Erythrocytes; V. Culture Systems; VI. Synchronization , VII. Gametocyte Production in CultureVIII. Cloning Methods; IX. Cryopreservation; X. Serum Replacement; XI. Axenic (Extracellular) Development of Erythrocytic Stages of P. falciparum; XII. Cultivation of Erythrocytic Stages of Other Species of Malaria Parasites; XIII. In Vitro Development of Preerythrocytic and Sporogonic Stages of the Life Cycle; References; Chapter 3. Molecular Tools for Genetic Dissection of the Protozoan Parasite Toxoplasma gondii; I. Introductory Overview; II. In Vitro Culture of T. gondii Tachyzoites; III. Molecular Transformation Systems for Toxoplasma , IV. Summary and OutlookReferences; Chapter 4. Transfection Experiments with Leishmania; I. Introduction; II. Stable and Transient Transfection; III. Transfection; References; Chapter 5. Mutagenesis and Variant Selection in Salmonella; I. Introduction; II. Mutagenesis; III. Screening of Variants; IV. Genetic Analysis; V. Perspectives; References; Chapter 6. Mycobacterium: Isolation, Maintenance, Transformation, and Mutant Selection; I. Introduction; II. Biosafety Considerations; III. Culture Media and Conditions; IV. Isolation of Mycobacteria; V. Maintenance of Stocks; VI. Genetic Techniques , VII. MutagenesisVIII. Strain Construction; IX. Mutant Selection and Isolation; References; Part II: Microbial Adherence and Invasion Assays; Chapter 7. Modulation of Murine Macrophage Behavior in Vivo and in Vitro; I. Introduction; II. Reagents and Solutions; III. Animal Husbandry and Maintenance of Immunocompromised Mice; IV. Eliciting and Harvesting Macrophages in Vivo; V. Adherence of Macrophages to Solid Substrates; VI. Assays for Macrophage Phagocytic Activity; VII. Analysis of MHC Class II Antigen Expression; References , Chapter 8. In Vitro Assays of Phagocytic Function of Human Peripheral Blood Leukocytes: Receptor Modulation and Signal TransductionI. Introduction; II. Phagocytosis Assays; III. Phagocytic Receptors; IV. Stimulation of Phagocytosis; V. Phagosome Isolation; References; Chapter 9. Bacterial Adhesion and Colonization Assays; I. Introduction; II. In Situ Screening of Host Receptor Distribution; III. Bacterial Adherence to Cells in Culture; IV. Biochemical Characterization of the Molecular Nature of Receptors in Situ; V. Bacterial Inhibition Experiments in Situ , VI. In Vitro Assays for Bacterial Adhesion , English

Additional Edition: ISBN 0-12-564146-X

Language: English

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Format: 1 online resource (586 p.)

ISBN: 1-283-03436-0 , 9786613034366 , 1-55581-665-7

Content: Featuring contributions from eminent immunologists, microbial geneticists, and cell and molecular biologists, this single volume brings together a current understanding of how phagocytes recognize and respond to potentially pathogenic microbes. It explores and explains the complex biology underlying the different phagocyte lineages that enables them to sense and respond to their environments.

Note: Description based upon print version of record. , Contents; Contributors; Foreword: What Would Darwin Have Said?; Preface; SECTION I: BIOLOGY OF THE PROFESSIONAL PHAGOCYTE; SECTION II: MEMBRANE RECEPTORS: RECOGNITION, ADHESION, PHAGOCYTOSIS, CHEMOTAXIS, AND MIGRATION; SECTION III: PHAGOCYTOSIS: SIGNALING, CYTOSKELETON, AND THE PHAGOSOME; SECTION IV: BRIDGING THE GAP BETWEEN THE INNATE AND ACQUIRED IMMUNE RESPONSE; SECTION V: PATHOGENS OF THE PROFESSIONAL PHAGOCYTE; SECTION VI: MODELS OF HOST-PATHOGEN INTERACTIONS; Index , English

Additional Edition: ISBN 1-55581-401-8

Language: English

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Format: 1 videorecording (53 min., 04 sec.) , 005304

Note: Retrieved April 12, 2024, from https://hstalks.com/bs/1309/. , Introduction -- Mycobacterium tuberculosis: the sobering facts -- The natural history of M. tuberculosis infection -- Immune control -- Controller vs. permissive phagocytes -- A fluorescent Mtb reporter strain for NO -- SSB-GFP fusion replication reporter -- Host cell heterogeneity impacts the bacterial burden -- Mtb is located in resident alveolar and recruited interstitial macrophages -- Expression of hspX-promoter-driven GFP (NO-mediated stress) -- Bacterial replication is higher in AM than in IM -- Impact of phagocyte populations of bacterial fitness -- The metabolic programming of the AM and IM lineages is markedly different -- Selective intoxication of IMs leads to expansion of the bacterial burden -- Impact of 2-DG & Etomoxir on bacterial growth inside macrophages in culture -- Mtb infection drives proliferation of uninfected, iNOS-ve macrophages -- Re-programming vs. pre-programming -- The metabolic state of the macrophages -- Metabolic flux experiment to demonstrate macrophage imprinting -- Profiling TB disease control and progression across species -- Dual RNA-seq of host and pathogen of in vivo infection -- Host cell and Mtb exhibit different transcriptional profiles -- Major cellular networks up-regulated in AMs and in IMs -- Differential host and bacterial responses to iron -- Mtb lives in a vacuole - where does the iron come from? -- Mycobacterial spp. modulate phagosome acidification to promote survival -- Transferrin cycles through the Mtb-containing vacuole -- EM autoradiography shows iron accumulation in Mtb -- Impact of immune status on iron acquisition & storage gene expression -- The impact of the host environment on drug action -- Screening in the host environment for 'smarter' antibiotics -- Summary of the hit compounds -- Carbon source influences compound activity in vitro -- New cholesterol-dependent inhibitors -- In vivo activity of an adenylate cyclase (rv1625c) active compound -- Host cell immune status impacts drug susceptibility -- A rational approach for identification of host-directed therapies -- Drugs and vaccines do not work independently -- Proposed workflow for analysis of metabolism of Mtb in vivo -- Co-incidence of HIV and TB infection in Sub-Saharan Africa -- How does HIV-1 impact human lung immunity? -- HIV-1 is preferentially located in the small alveolar macrophages -- HIV-1 does not trigger cell death in HIV-1-infected macrophages -- Regulatory lncRNAs are differentially induced by HIV-1 -- lncRNA-SAF expression is upregulated in HIV-1-positive AMs from humans -- Down-regulation of lncRNA-SAF leads to cell death in infected macrophages -- Induction of cell death shuts down viral productivity -- Take-home messages -- Thank you for listening.

Language: English