UID:
almahu_9948026249702882
Format:
1 online resource (613 p.)
ISBN:
1-281-04640-X
,
9786611046408
,
0-08-053450-3
Content:
Immunoassays are among the most powerful and sensitive technologies now available for patient diagnosis and monitoring. This book is an indispensable guide to information on the theory and practice of immunoassays. It discusses the scientific basis of these technologies in a logical, organized, and heuristic manner and provides protocols for specific assays. The contents of this unique book are balanced among theory, practical issues, quality control, automation, and subspecialty areas, making it ideal for health science students, laboratory scientists, and clinicians.Key Features*
Note:
Description based upon print version of record.
,
Front Cover; Immunoassay; Copyright Page; Contents; Contributors; Preface; CHAPTER 1. PAST, PRESENT AND FUTURE OF IMMUNOASSAYS; CHAPTER 2. IMMUNE FUNCTION AND ANTIBODY STRUCTURE; 1. Cellular and Molecular Basis of the Humoral Immune Response; 2. Antibody Structure; 3. Antigen Binding; 4. Antibody Structure and Immunologic Diversity; 5. Conclusion; CHAPTER 3. THEORY OF IMMUNOASSAYS; 1. Binding Theory; 2. Equilibria Involving Many Ligands and a Heterogeneous Population of Binding Sites; 3. Principles of Saturation Analysis; 4. Kinetics of Immunoassay; 5. The Theory of Two-Site Immunoassays
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6. Graphical Presentation of Immunoassay Data7. Curve Fitting of Immunoassay Data; 8. Detection Limit of Immunoassays; References; CHAPTER 4. DATA INTERPRETATION AND QUALITY CONTROL; 1. Introduction; 2. Assay Validation; 3. Determining Immunoassay Sensitivity; 4. Tests of Similarity (Parallelism); 5. Alternative Methods for Defining Cross Reactivity; 6. Quality Control; 7. Summary; References; CHAPTER 5. PRODUCTION AND PURIFICATION OF ANTIBODIES; 1. Introduction; 2. Polyclonal versus Monoclonal Antibodies; 3. Choice of Animal; 4. Immunization Procedures
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5. Obtaining and Processing Blood and Lymphocytes6. Monoclonal Antibody Production; 7. Antibody Purification; References; CHAPTER 6. ANTIBODY ENGINEERING; 1. Introduction; 2. Cloning Antibody Variable Region Genes; 3. Expression of Recombinant Antibodies; 4. Antibody Structure-Function Studies; 5. Therapeutic Antibodies; 6. Designer Antibodies; 7. Future Directions; References; CHAPTER 7. INTERFERENCES IN IMMUNOASSAYS; 1. Introduction; 2. Detecting Interferences; 3. Cross-Reactivity and Heterogeneous Analytes; 4. Reducing Cross-Reactive Interferences; 5. Heterophile and Anti-Animal Antibodies
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6. Recognizing and Reducing Interference from Endogenous Antibodies7. Interferences Due to Masking of Antigens; 8. Interference with the Indicator Mechanism; 9. Matrix Effects; 10. Interference Problems in the Future; References; CHAPTER 8. LABELING OF ANTIBODIES AND ANTIGENS; 1. Introduction; 2. Requirements for Quality of Labeled Reactants; 3. Chemical Reactions for Conjugation; 4. Reagents for Conjugation; 5. Conjugation of Antibody IgG to Enzymes; 6. Conjugation of Antibody Fragments to Enzymes; 7. Conjugation of Antigens, Avidin, and Streptavidin to Enzymes
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8. Conjugation of Small Molecules to Antibodies and AntigensReferences; CHAPTER 9. SOLID PHASES IN IMMUNOASSAY; 1. Interfacial Immunochemistry; 2. Diversity of Solid Phases; 3. Immobilization Procedures; 4. SPI in the Future; References; CHAPTER 10. IMMUNOASSAY CONFIGURATIONS; 1. Introduction; 2. Competitive Immunoassays; 3. Noncompetitive Immunoassays; 4. Indirect Detection of the Immunocomplexes; References; CHAPTER 11. THE AVIDIN-BIOTIN SYSTEM; 1. Introduction; 2. Components of the System; 3. Amplified Detection Systems; 4. Improvement of Capture Systems; 5. Auxiliary Enhancement Systems
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6. Selected Protocols
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English
Additional Edition:
ISBN 0-12-214730-8
Language:
English
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