Journal of Chromatography A, July 4, 2014, Vol.1349, p.96(9)
To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.chroma.2014.04.087 Byline: David Gaddes, Jessica Westland, Frank L. Dorman, Srinivas Tadigadapa Abstract: This work focuses on the development and experimental evaluation of micromachined chromatographic columns for use in a commercial gas chromatography (GC) system. A vespel/graphite ferrule based compression sealing technique is presented using which leak-proof fluidic interconnection between the inlet tubing and the microchannel was achieved. This sealing technique enabled separation at temperatures up to 350[degrees]C on a [mu]GC column. This paper reports the first high-temperature separations in microfabricated chromatographic columns at these temperatures. A 2m microfabricated column using a double Archimedean spiral design with a square cross-section of 100[mu]mx100[mu]m has been developed using silicon microfabrication techniques. The microfabricated column was benchmarked against a 2m 100[mu]m diameter commercial column and the performance between the two columns was evaluated in tests performed under identical conditions. High temperature separations of simulated distillation (ASTM2887) and polycyclic aromatic hydrocarbons (EPA8310) were performed using the [mu]GC column in temperature programmed mode. The demonstrated [mu]GC column along with the high temperature fixture offers one more solution toward potentially realizing a portable [mu]GC device for the detection of semi-volatile environmental pollutants and explosives without the thermal limitations reported to date with [mu]GC columns using epoxy based interconnect technology. Author Affiliation: (a) Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA 16802, USA (b) Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA (c) Department of Electrical Engineering, The Pennsylvania State University, University Park, PA 16802, USA Article History: Received 14 February 2014; Revised 24 April 2014; Accepted 25 April 2014
Biomedical Engineering ; Epoxy Resins ; Polycyclic Aromatic Hydrocarbons ; Gas Chromatography
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