Format:
Online-Ressource (X, 144 p. 47 illus., 40 illus. in color, digital)
ISBN:
9783642295546
,
1283944855
,
9781283944854
Series Statement:
Springer Series on Biofilms 7
Content:
During the recent transition between acute diseases caused by swarms of single planktonic bacteria, and chronic infections caused by bacteria growing in slime-enclosed biofilms, a general clinical consensus has emerged that pathologies with bacterial etiologies are frequently culture negative. Because biofilm infections now affect 17 million Americans per year (killing approximately 450,000), the suggestion that these common and lethal infections regularly go unnoticed by the only FDA-approved method for their detection and characterization is a matter of urgent concern. Biologically, we would expect that planktonic bacterial cells would colonize any new surface, including the surface of an agar plate, while the specialized sessile cells of a biofilm community would have no such proclivity. In the study of biofilm diseases ranging from otitis media to prostatitis, it was found that direct microscopy and DNA- and RNA-based molecular methods regularly document the presence of living bacteria in tissues and samples that are culture negative. The editors selected orthopedic biofilm infections as the subject of this book because these infections occur against a background of microbiological sterility in which modern molecular methods would be expected to find bacterial DNA, RNA-based microscopic methods would be expected to locate bacterial cells, and cultures would be negative. Moreover, in Orthopedics we find an already biofilm-adapted surgical group in which current strategies are based on the meticulous removal of compromised tissues, antibiotic options as based on high biofilm-killing local doses, and there are practical bedside strategies for dealing with biofilm infections. So here is where the new paradigm of biofilm infection meets the equally new paradigm of the culture negativity of biofilms, and this volume presents a conceptual synthesis that may soon combine the most effective molecular methods for the detection and identification of bacteria with a surgical discipline that is ready to help patients.
Note:
Description based upon print version of record
,
Culture Negative Orthopedic Biofilm Infections; In Memoriam; Preface; Contents; The Problem of Culture-Negative Infections; 1 Silos in Clinical Medicine; 2 The Rational Basis of the Problem of Culture Negativity; 3 Molecular Answers to the Culture-Negative Problem; 4 Multiple Paradigm Shifts; 5 Historical Origins of the Problem of Culturability; 6 Two Solitudes; References; Culture-Negative Infections in Orthopedic Surgery; 1 Culture Methods for Bacterial Detection and Identification; 2 The Failure of Culture Methods in the Detection of Chronic Biofilm Infections
,
3 The Solution to the Problem of Negative Cultures4 The IBIS PLEX-ID; 5 Summary; References; Improved Diagnosis of Biofilm Infections Using Various Molecular Methods; 1 Introduction; 2 Overview of Molecular Techniques for Chronic Infection Evaluation; 2.1 Polymerase Chain Reaction; 2.2 Fluorescence In Situ Hybridization; 2.3 Fingerprinting; 2.4 Clone Library; 3 Molecular Techniques Highlight Biofilm Diversity in Chronic Wounds; 3.1 Diversity Identification is Maximized by Evaluating Culture and Culture-Independent Techniques; 3.2 Molecular Techniques Reinforce Sampling Requirements
,
4 Applying Molecular Techniques to Prosthetic Joint Infection4.1 Methodology Considerations; 4.2 Preliminary Results; 4.3 Case Presentation; 5 Conclusion; References; Improved Outcomes Via Integrated Molecular Diagnostics and Biofilm Targeted Therapeutics; 1 Introduction; 2 Biofilm-Based Treatment Strategies for Chronic Infection; 2.1 Physical Removal/Debridement; 2.2 Accurate Diagnostics; 2.2.1 Limitations of Traditional Culturing Techniques and Sensitivity Testing; 2.2.2 Molecular Diagnostics: PCR in Combination with PSEQ; 2.3 Antibiofilm Agents; 2.4 Antibiotics
,
3 Outcomes Example: A Fully Leveraged Four-Leg Approach3.1 DNA-Identification to Define Chronic Wound Pathogens; 3.2 Clinical Outcomes Driven by DNA-Guided Personalized Medicine; 4 Conclusion; References; Immunological Methods for Staphylococcus aureus Infection Diagnosis and Prevention; 1 Introduction to Staphylococcus aureus and Biofilms; 2 Immune Evasion from the Bacterial and Host Perspective; 2.1 Immune Evasion Strategies of S. aureus; 2.2 Host Immune Response to Biofilm Infection; 3 Incorporating the Biofilm Perspective into Vaccine Development and Translational Research
,
3.1 Growing In Vitro S. aureus Biofilms for Analysis3.2 Anti-Biofilm Vaccine Development; 3.3 Rapid Diagnosis Using Lateral Flow Immunoassay; 4 Conclusion; References; Diagnosing Periprosthetic Joint Infection: Cultures, Molecular Markers, and the Ibis Technology; 1 Introduction; 2 Imperative to Identify PJI; 3 Traditional Markers of PJI; 3.1 Periprosthetic Cultures; 3.2 Inflammatory Markers for Diagnosing Periprosthetic Joint Infections; 4 New Technologies for Detecting PJI; 4.1 Molecular Markers; 4.2 Ibis Technology; 4.3 Molecular Markers and Ibis Technology
,
5 Future Directions for Diagnosing PJI
,
The Problem of Culture Negative Infections -- Culture Negative Infections in Orthopaedic Surgery -- Improved Diagnosis of Biofilm Infections Using Various Molecular Methods -- Improved Outcomes via Integrated Molecular Diagnostics and Biofilm Targeted Therapy -- Immunological Methods for Staphylococcus aureus Infection Diagnosis and Prevention -- Diagnosing Periprosthetic Joint Infections : Cultures, Molecular Markers, and the Ibis technology -- Infections Associated with Severe Open Tibia Fractures -- Second Generation Molecular Diagnostics and Strategies for Preventing Periprosthetic Joint Infections -- Treatment of Orthopedic Infections : Addressing the Biofilm Issue -- Valete : Towards a New Paradigm in the Diagnosis and Treatment of Orthopedic Infections..
Additional Edition:
ISBN 9783642295539
Additional Edition:
Buchausg. u.d.T. Culture negative orthopedic biofilm infections Berlin : Springer, 2012 ISBN 9783642295539
Additional Edition:
ISBN 3642295533
Language:
English
Subjects:
Medicine
Keywords:
Bewegungsapparat
;
Bakterielle Infektion
;
Biofilm
;
Aufsatzsammlung
DOI:
10.1007/978-3-642-29554-6
URL:
Volltext
(lizenzpflichtig)
Bookmarklink
