Soil Biology and Biochemistry, 2015, Vol.84, p.21(7)
To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.soilbio.2015.02.010 Byline: Hannes Schmidt, Doris Vetterlein, John M. Kohne, Thilo Eickhorst Abstract: X-ray Microfocused Computed Tomography (X-ray [mu]-CT) allows a non-destructive and three-dimensional observation of microbial habitats (i.e. pore space) in soil. A major premise for microbiological studies integrating X-ray [mu]-CT is that soil microorganisms are not affected by irradiation dose in terms of physiology and composition. However, the compatibility of X-ray [mu]-CT and soil biological experiments has been evaluated controversially. We performed an incubation experiment with packed microcosms to assess the effect of X-ray [mu]-CT on native microbial populations with emphasis on soil archaea and bacteria. Before (14 days) and after (1 and 14 days) scanning we analyzed (i) respiration, (ii) enzyme activity, (iii) microbial biomass, (iv) abundance and (v) community structure in scanned and control treatments. None of the microbial parameters exhibited significant differences among scanned and unscanned soil samples at all sampling times with the exception of lower archaeal cell numbers subsequent to X-ray [mu]-CT. Incubation time was the main factor that induced a significant alteration of microbial soil populations while irradiation had no or only very little effect thereupon. Taken together, three-dimensional in situ data obtained via X-ray [mu]-CT may well be combined with microbiological analyses in soil. Author Affiliation: (a) Soil Microbial Ecology, University of Bremen, 28359 Bremen, Germany (b) Department of Microbiology and Ecosystem Science, Division of Microbial Ecology, University of Vienna, 1090 Vienna, Austria (c) Department of Soil Physics, UFZ - Helmholtz Center for Environmental Research, 06120 Halle, Germany Article History: Received 18 November 2014; Revised 7 February 2015; Accepted 9 February 2015
Enzymes – Physiological Aspects ; Enzymes – Analysis ; Soil Microbiology – Physiological Aspects ; Soil Microbiology – Analysis ; Bacteria – Physiological Aspects ; Bacteria – Analysis ; Cat Scans – Physiological Aspects ; Cat Scans – Analysis ; Ecosystems – Physiological Aspects ; Ecosystems – Analysis
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