Kooperativer Bibliotheksverbund

UID:

Format: 1 online resource : , illustrations

ISBN: 9783527655946 , 3527655948 , 9783527655977 , 3527655972 , 9781299178762 , 1299178766

Note: Machine generated contents note: 1. Lipidomics Perspective: From Molecular Lipidomics to Validated Clinical Diagnostics / Kim Ekroos -- 1.1. Introduction -- 1.2. Hierarchical Categorization of the Analytical Lipid Outputs -- 1.2.1. Lipid Class -- 1.2.2. Sum Compositions -- 1.2.3. Molecular Lipids -- 1.2.4. Structurally Defined Molecular Lipids -- 1.3. The Type of Lipid Information Delivers Different Biological Knowledge -- 1.4. Untying New Biological Evidences through Molecular Lipidomic Applications -- 1.5. Molecular Lipidomics Approaches Clinical Diagnostics -- 1.6. Current Roadblocks in Lipidomics -- 1.7. Conclusions -- References -- 2. Lipids in Cells / Michal Surma -- 2.1. Introduction -- 2.2. Basis of Cellular Lipid Distribution -- 2.3. Lipid Distribution by Nonvesicular Routes -- 2.4. Lipids in Different Cell Types -- 2.5. Functional Implications of Membrane Lipid Composition -- 2.6. Outlook: Collectives and Phase Separation -- References -- 3. High-Throughput Molecular Lipidomics / Kim Ekroos -- 3.1. Introduction -- 3.2. Lipid Diversity -- 3.3. Function of Molecular Lipids -- 3.4. Automated Sample Preparation -- 3.5. Different Approaches to Molecular Lipidomics -- 3.5.1. Untargeted versus Targeted Approaches -- 3.5.2. Shotgun Lipidomics -- 3.5.3. Analytical Validation of the Shotgun Approach -- 3.5.4. Targeted LC-MS Lipidomics -- 3.6. Data Processing and Evaluation -- 3.7. Lipidomic Workflows -- 3.8. Conclusions and Future Perspectives -- References -- 4. Multidimensional Mass Spectrometry-Based Shotgun Lipidomics / Xianlin Han -- 4.1. Introduction -- 4.2. Multidimensional Mass Spectrometry-Based Shotgun Lipidomics -- 4.2.1. Intrasource Separation -- 4.2.2. The Principle of Multidimensional Mass Spectrometry -- 4.2.3. Variables in Multidimensional Mass Spectrometry -- 4.2.3.1. Variables in Fragment Monitoring by Tandem MS Scans -- 4.2.3.2. Variables Related to the Infusion Conditions -- 4.2.3.3. Variables under Ionization Conditions -- 4.2.3.4. Variables under Collision Conditions -- 4.2.3.5. Variables Related to the Sample Preparations -- 4.3. Application of Multidimensional Mass Spectrometry-Based Shotgun Lipidomics for Lipidomic Analysis -- 4.3.1. Identification of Lipid Molecular Species by 2D Mass Spectrometry -- 4.3.1.1. Identification of Anionic Lipids -- 4.3.1.2. Identification of Weakly Anionic Lipids -- 4.3.1.3. Identification of Charge Neutral but Polar Lipids -- 4.3.1.4. Identification of Sphingolipids -- 4.3.1.5. The Concerns of the MDMS-Based Shotgun Lipidomics for Identification of Lipid Species -- 4.3.2. Quantification of Lipid Molecular Species by MDMS-Based Shotgun Lipidomics -- 4.3.2.1. The Principle of Quantification of Individual Lipid Species by MS -- 4.3.2.2. Quantification by Using a Two-Step Procedure in MDMS-Based Shotgun Lipidomics -- 4.3.2.3. Quantitative Analysis of PEX7 Mouse Brain Lipidome by MDMS-Based Shotgun Lipidomics -- 4.4. Conclusions -- References -- 5. Targeted Lipidomics: Sphingolipidomics / M. Cameron Sullards -- 5.1. Introduction -- 5.2. Sphingolipids Description and Nomenclature -- 5.3. Sphingolipids Analysis via Targeted LC-MS/MS -- 5.3.1. Sphingolipid Internal Standards -- 5.3.2. Biological Sample Preparation and Storage -- 5.3.3. Sphingolipid Extraction Protocol -- 5.3.4. Liquid Chromatography -- 5.3.4.1. LCBs and Cer1P -- 5.3.4.2. Cer, HexCer, LacCer, SM, ST, and Cer1P -- 5.3.4.3. Separation of GlcCer and GalCer -- 5.3.5. Mass Spectrometry -- 5.3.5.1. Electrospray Ionization -- 5.3.5.2. Tandem Mass Spectrometry -- 5.3.5.3. Multiple Reaction Monitoring -- 5.3.6. Generation of Standard Curves -- 5.3.7. Data Analysis -- 5.3.8. Quality Control -- 5.4. Applications of Sphingolipidomics in Biology and Disease -- 5.4.1. LC-MS/MS -- 5.4.2. Transcriptomic Guided Tissue Imaging Mass Spectrometry -- 5.5. Conclusions -- References -- 6. Structural Lipidomics / Stephen J. Blanksby -- 6.1. Introduction -- 6.2. Lipid Structure -- 6.3. Structural Analysis of Lipids by Mass Spectrometry -- 6.4.sn Position -- 6.5. Double Bond Position -- 6.5.1. Untargeted Fragmentation -- 6.5.2. Targeted Fragmentation -- 6.6. Double Bond Stereochemistry -- 6.7. Conclusions -- References -- 7. Imaging Lipids in Tissues by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry / Robert C. Murphy -- 7.1. Introduction -- 7.2. Sample Preparation -- 7.3. Matrix -- 7.3.1. Techniques for Matrix Application -- 7.3.2. Matrix Compounds -- 7.4. Instrumentation -- 7.4.1. Lasers and Rastering -- 7.4.2. Ion Formation -- 7.4.3. Mass Analyzers and Ion Detection -- 7.5. Data Processing -- 7.6. Conclusions -- References -- 8. Lipid Informatics: From a Mass Spectrum to Interactomics / Kirill Tarasov -- 8.1. Introduction -- 8.2. Lipid Nomenclature -- 8.3. Basic Properties of Lipid Mass spectrometric Data -- 8.3.1. Mass Spectrum -- 8.3.2. Mass Accuracy and Reproducibility -- 8.3.3. Isotopes, Deisotoping, and Isotope Correction -- 8.4. Data Processing -- 8.4.1. De Novo Lipid Identification -- 8.4.2. Targeted Export of Lipidomic Data -- 8.4.3. Normalization of lipidomic Data -- 8.5. Lipidomic Data Mining and Visualization -- 8.5.1.Comparative Lipidomics -- 8.5.2. Multivariate Data Analysis -- 8.5.3. Lipidomics in Biomarker Research -- 8.6. Lipidomic Data Integration -- 8.7. Conclusions and Future Perspectives -- References -- 9. Lipids in Human Diseases / Scott A. , Summers -- 9.1. Introduction -- 9.2. Obesity -- 9.3. Dyslipidemia -- 9.4. Diabetes -- 9.5. Cardiovascular Disorders -- 9.6. Hereditary Sensory Neuropathy -- 9.7. Neurodegeneration -- 9.8. Cancer -- 9.9. Lysosomal Storage Disorders -- 9.10. Cystic Fibrosis -- 9.11. Anti-Inflammatory Lipid Mediators -- 9.12. Conclusions -- References -- 10. Lipidomics in Lipoprotein Biology / Anatol Kontush -- 10.1. Introduction -- 10.2. Metabolism of Lipoproteins -- 10.3. Lipoproteinomics in Normolipidemic Subjects -- 10.3.1. Phospholipids -- 10.3.1.1. Phosphatidylcholine -- 10.3.1.2. Lysophosphatidylcholine -- 10.3.1.3. Phosphatidylethanolamine -- 10.3.1.4. Phosphatidylethanolamine Plasmalogens -- 10.3.1.5. Phosphatidylinositol, Phosphatidylserine, Phosphatidylglycerol, and Phosphatidic Acid -- 10.3.1.6. Cardiolipin -- 10.3.1.7. Isoprostane-Containing PC -- 10.3.2. Sphingolipids -- 10.3.2.1. Sphingomyelin -- 10.3.2.2. Lysosphingolipids -- 10.3.2.3. Ceramide -- 10.3.2.4. Minor Sphingolipids -- 10.3.3. Sterols -- 10.3.4. Cholesteryl Esters -- 10.3.5. Triacylglycerides -- 10.3.6. Minor Lipids -- 10.4. Altered Lipoproteinomics in Dyslipidemia -- 10.4.1. Phospholipids -- 10.4.1.1. Phosphatidylcholine -- 10.4.1.2. Lysophosphatidylcholine -- 10.4.1.3. Phosphatidylethanolamine -- 10.4.1.4. Phosphatidylethanolamine Plasmalogens -- 10.4.1.5. Phosphatidylinositol -- 10.4.1.6. Isoprostane-Containing PC -- 10.4.2. Sphingolipids -- 10.4.2.1. Sphingomyelin -- 10.4.2.2. Lysosphingolipids: S1P and Dihydro S1P -- 10.4.2.3. Ceramide -- 10.4.3. Free Cholesterol -- 10.4.4. Cholesteryl Esters -- 10.4.5. Triacylglycerides -- 10.4.6. Minor Lipids -- 10.4.6.1. Nonesterified Fatty Acids -- 10.4.6.2. Ganglioside GM1 -- 10.4.6.3. Oxidized Lipids -- 10.5. Conclusions -- References -- 11. Mediator Lipidomics in Inflammation Research / Yosuke Isobe -- 11.1. Introduction -- 11.2. PUFA-Derived Lipid Mediators: Formation and Action -- 11.3. LC-ESI-MS/MS-Based Lipidomics -- 11.3.1. Sample Preparation -- 11.3.2. LC-ESI-MS/MS Analysis -- 11.4. Mediator Lipidomics in Inflammation and Resolution -- 11.5. Conclusion and Future Perspective -- References -- 12. Lipidomics for Elucidation of Metabolic Syndrome and Related Lipid Metabolic Disorder / Hiroki Nakanishi -- 12.1. Introduction -- 12.2. Basic Strategy of Lipidomics for Elucidating Metabolic Changes of Lipids at the Level of their Molecular Species in Metabolic Syndrome and Related Diseases -- 12.3. Analytical Systems by Mass Spectrometry in Lipidomics -- 12.3.1. LC-MS and LC-MS/MS Analyses for Global Detection of Phospholipids and Triglycerides -- 12.3.2. Infusion Analysis with Precursor Ion and Neutral Loss Scanning -- 12.3.3. Targeted Analysis by Multiple Reaction Monitoring for Oxidized Lipids and Lipid Mediators by LC-MS/MS on Triple-Stage Quadrupole Mass Spectrometers -- 12.4. Lipidomic Data Processing -- 12.4.1. Strategy of Lipid Search -- 12.4.2. Application and Identification Results of "Lipid Search" -- 12.5. Analysis of Lipids as Markers of Metabolic Syndrome -- 12.5.1. Oxidized Phospholipids -- 12.5.1.1. Application for Myocardial Ischemia-Reperfusion Model -- 12.5.2. Bioactive Acidic Phospholipids -- 12.5.2.1. Lysophosphatidic Acid -- 12.5.2.2. Phosphoinositides -- 12.5.3. Oxidative Triglycerides -- 12.5.3.1. Application for Mouse White Adipose Tissue -- 12.5.4. Sphingolipids -- 12.5.4.1. Application for Sphinogolipid Metabolism -- 12.6. Direct Detection of Lipid Molecular Species in Specific Tissue Domains by Disease-Specific Changes -- 12.7. Conclusions -- References -- 13. Lipidomics in Atherosclerotic Vascular Disease / Reijo Laaksonen -- 13.1. Introduction -- 13.2. Lipids and Atherosclerotic Vascular Disease -- 13.2.1. Lipoproteins -- 13.2.2. Atherosclerotic Plaque -- 13.2.3. Molecular Lipids -- 13.2.3.1. Eicosanoids -- 13.2.3.2. Sphingolipids and Cholesterol -- 13.2.3.3. Phospholipids -- 13.2.4. Animal Models of Atherosclerotic Research -- 13.3. Diagnostics and Treatment -- 13.3.1. Diagnostic Biomarkers of Atherosclerosis -- 13.3.2. Lipidomics in Efficacy and Safety Measurements -- 13.4. Conclusions -- References -- 14. Lipid Metabolism in Neurodegenerative Diseases / Markus R. Wenk -- 14.1. Introduction -- 14.1.1. Brain Lipids -- 14.1.2. Mass Spectrometry of Brain Lipids -- 14.2. Alzheimer's Disease -- 14.2.1. Cholesterol and Cholesterol Esters -- 14.2.2. Sulfatides -- 14.2.3. Plasmalogen Ethanolamines -- 14.2.4. Phospholipases -- 14.2.4.1. Phospholipase A2 -- 14.2.4.2. Phospholipase C and Phospholipase D -- 14.3. Parkinson's Disease -- 14.3.1. Cerebrosides -- 14.3.2. Coenzyme Q -- 14.3.3. Endocannabinoids -- 14.4. Conclusions -- References -- 15. The Tumor Mitochondrial Lipidome and Respiratory Bioenergetic Insufficiency / Michael A. Kiebish -- 15.1. Introduction -- 15.1.1. Lipidomic Abnormalities in Tumor Mitochondria.

Additional Edition: Print version: Lipidomics. Weinheim : Wiley-VCH, ©2012 ISBN 9783527330980

Language: English

Subjects: Chemistry/Pharmacy , Biology , Medicine

Keywords: Electronic books. ; Aufsatzsammlung ; Electronic books. ; Electronic books.

DOI: 10.1002/9783527655946

URL: https://onlinelibrary.wiley.com/doi/book/10.1002/9783527655946

URL: Volltext

URL: https://onlinelibrary.wiley.com/doi/book/10.1002/9783527655946

URL: https://onlinelibrary.wiley.com/doi/book/10.1002/9783527655946

UID:

Format: XX, 336 S. : , Ill., graph. Darst. ; , 25 cm.

ISBN: 978-3-527-33098-0

Note: Literaturangaben

Language: English

Subjects: Chemistry/Pharmacy , Biology , Medicine

Keywords: Lipidomik ; Aufsatzsammlung ; Aufsatzsammlung

URL: Inhaltstext

URL: Inhaltsverzeichnis

URL: Inhaltsverzeichnis

URL: Inhaltstext

URL: Cover

UID:

Format: 1 online resource (750 pages)

ISBN: 9783527836499 , 3527836497 , 3527836500 , 9783527836505 , 9783527836512 , 3527836519

Note: 4.2.2 Hydrophilic Interaction Liquid Chromatography , Cover -- Volume 1 -- Title Page -- Copyright Page -- Contents -- Preface -- Chapter 1 Introduction to Lipidomics -- 1.1 Preface -- 1.2 Historical Perspective -- 1.3 Sampling and Preanalytics -- 1.4 Reference Materials and Biological Reference Ranges -- 1.5 Clinical Lipidomics -- 1.6 Identification and Annotation -- 1.7 Quantitation -- 1.8 Lipid Ontology -- References -- Part I Analytical Methodologies in Lipidomics -- Chapter 2 Preanalytics for Lipidomics Analysis -- 2.1 Safety -- 2.2 Introduction -- 2.3 Sample Origin -- 2.4 Sample Collection -- 2.5 Tissue Homogenization , 2.5.1 Mortar and Pestle -- 2.5.2 Rotor-Stator -- 2.5.3 Blender -- 2.5.4 Potter-Elvehjem -- 2.5.5 Bead Mill -- 2.6 Liquid-Liquid Extraction (LLE) -- 2.6.1 Folch Method -- 2.6.2 Bligh and Dyer (BD) Method -- 2.6.3 Modified Folch and Bligh/Dyer (BD) Methods -- 2.6.4 Rose and Oaklander (RO) Method -- 2.6.5 Matyash or Methyl-tert-Butyl Ether (MTBE) Method -- 2.6.6 BUME Method -- 2.6.7 Alshehry Method -- 2.6.8 Three-Phase Lipid Extraction (3PLE) -- 2.7 Resuspension and Solubilization -- 2.8 Automation -- 2.9 Tips and Tricks -- Acknowledgments -- References , Chapter 3 Direct Infusion (Shotgun) Electrospray Mass Spectrometry -- 3.1 Introduction -- 3.2 Complexity of Crude Lipid Extracts -- 3.2.1 Main Lipid Classes in Mammalian Samples -- 3.2.2 Bond Types as Structural Features -- 3.2.3 Fatty Acids as the Major Building Blocks -- 3.2.4 Lipid Species and Double-Bond Series -- 3.3 Introduction to Mass Spectrometry of Lipids -- 3.3.1 Annotation of Lipid Structures Analyzed by MS -- 3.3.2 Isomers -- 3.3.3 Isobars and the Type-II Isotopic Overlap -- 3.4 Overview of Direct Infusion MS Workflows -- 3.5 Sample Preparation , 3.5.1 Preanalytics - Sample Stability -- 3.5.2 Lipid Extraction -- 3.5.3 Solvents, Additives, and Lipid Concentration -- 3.5.4 Sample Derivatization -- 3.6 Direct Infusion -- 3.7 Mass Spectrometry Analysis -- 3.7.1 Electrospray Ionization of Lipids -- 3.7.2 Tandem Mass Spectrometry -- 3.7.3 Multidimensional MS Shotgun Lipidomics -- 3.7.4 High-Resolution Mass Spectrometry -- 3.8 Lipid Identification -- 3.8.1 Identification by MS/MS -- 3.8.2 Identification by HRMS -- 3.8.3 Consideration of Type-II Overlap -- 3.8.4 Identification Hierarchy -- 3.8.5 Caveats/Pitfalls -- 3.9 Lipid Quantification , 3.9.1 Internal Standards -- 3.9.2 Type-I Isotopic Effect -- 3.9.3 Evaluation and Correction of Isotopic Overlap -- 3.9.4 Species Response -- 3.9.5 Calculation of Concentration -- 3.10 Data Analysis/Software -- 3.11 Limitations -- 3.12 Selected Applications -- 3.12.1 Analysis of Plasma -- 3.12.2 Analysis of Tissues and Cells -- 3.12.3 Analysis of Lipid Metabolism -- 3.13 Outlook -- References -- Chapter 4 Liquid Chromatography -- and Supercritical Fluid Chromatography -- Mass Spectrometry -- 4.1 Introduction -- 4.2 Lipid Class Separation -- 4.2.1 Normal-Phase Liquid Chromatography

Additional Edition: Print version: Holcapek, Michal Mass Spectrometry for Lipidomics Newark : John Wiley & Sons, Incorporated,c2023 ISBN 9783527350155

Language: English

DOI: 10.1002/9783527836512

URL: https://onlinelibrary.wiley.com/doi/book/10.1002/9783527836512

URL: https://onlinelibrary.wiley.com/doi/book/10.1002/9783527836512

URL: https://onlinelibrary.wiley.com/doi/book/10.1002/9783527836512

UID:

Format: 1 Online-Ressource (2 Bände)

ISBN: 9783527836512 , 9783527836505

Content: Cover -- Volume 1 -- Title Page -- Copyright Page -- Contents -- Preface -- Chapter 1 Introduction to Lipidomics -- 1.1 Preface -- 1.2 Historical Perspective -- 1.3 Sampling and Preanalytics -- 1.4 Reference Materials and Biological Reference Ranges -- 1.5 Clinical Lipidomics -- 1.6 Identification and Annotation -- 1.7 Quantitation -- 1.8 Lipid Ontology -- References -- Part I Analytical Methodologies in Lipidomics -- Chapter 2 Preanalytics for Lipidomics Analysis -- 2.1 Safety -- 2.2 Introduction -- 2.3 Sample Origin -- 2.4 Sample Collection -- 2.5 Tissue Homogenization -- 2.5.1 Mortar and Pestle -- 2.5.2 Rotor-Stator -- 2.5.3 Blender -- 2.5.4 Potter-Elvehjem -- 2.5.5 Bead Mill -- 2.6 Liquid-Liquid Extraction (LLE) -- 2.6.1 Folch Method -- 2.6.2 Bligh and Dyer (BD) Method -- 2.6.3 Modified Folch and Bligh/Dyer (BD) Methods -- 2.6.4 Rose and Oaklander (RO) Method -- 2.6.5 Matyash or Methyl-tert-Butyl Ether (MTBE) Method -- 2.6.6 BUME Method -- 2.6.7 Alshehry Method -- 2.6.8 Three-Phase Lipid Extraction (3PLE) -- 2.7 Resuspension and Solubilization -- 2.8 Automation -- 2.9 Tips and Tricks -- Acknowledgments -- References -- Chapter 3 Direct Infusion (Shotgun) Electrospray Mass Spectrometry -- 3.1 Introduction -- 3.2 Complexity of Crude Lipid Extracts -- 3.2.1 Main Lipid Classes in Mammalian Samples -- 3.2.2 Bond Types as Structural Features -- 3.2.3 Fatty Acids as the Major Building Blocks -- 3.2.4 Lipid Species and Double-Bond Series -- 3.3 Introduction to Mass Spectrometry of Lipids -- 3.3.1 Annotation of Lipid Structures Analyzed by MS -- 3.3.2 Isomers -- 3.3.3 Isobars and the Type-II Isotopic Overlap -- 3.4 Overview of Direct Infusion MS Workflows -- 3.5 Sample Preparation -- 3.5.1 Preanalytics - Sample Stability -- 3.5.2 Lipid Extraction -- 3.5.3 Solvents, Additives, and Lipid Concentration -- 3.5.4 Sample Derivatization.

Additional Edition: Erscheint auch als Druck-Ausgabe ISBN 978-3-527-35015-5

Language: English

Subjects: Chemistry/Pharmacy

Keywords: Lipidomik ; Massenspektrometrie ; Lipide ; Aufsatzsammlung

DOI: 10.1002/9783527836512

URL: Volltext  (URL des Erstveröffentlichers)

UID:

ISBN: 978-3-527-35015-5

Additional Edition: Erscheint auch als Online-Ausgabe, PDF ISBN 978-3-527-83649-9

Additional Edition: Erscheint auch als Online-Ausgabe, EPUB ISBN 978-3-527-83650-5

Additional Edition: Erscheint auch als Online-Ausgabe, oBook ISBN 978-3-527-83651-2

Language: English

Subjects: Chemistry/Pharmacy

Keywords: Aufsatzsammlung

URL: Kurzbeschreibung

URL: Inhaltsverzeichnis