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  • 1
    Language: English
    In: European Journal of Cancer, Sept, 1997, Vol.33, p.S280
    Description: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/S0959-8049(97)86179-5 Byline: M. Lorenz, G. Janshon, S. Heinrich, H. Petrowsky, A. Encke
    Keywords: Pancreatic Cancer -- Care And Treatment ; Cancer Patients -- Care And Treatment
    ISSN: 0959-8049
    Source: Cengage Learning, Inc.
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  • 2
    In: Transplantation, 1995, Vol.59(7), pp.1023-1028
    Description: Expression of adhesion molecules and human leukocyte antigens on the surface of hepatocytes (HC) may play an important role in the immune reaction in different types of infectious and noninfectious hepatitis, liver graft rejection, and autoimmune liver diseases.The aim of this study was to evaluate the influence of the proinflammatory cytokines IFN-α, IFN-γ, and IL-1α on the expression of intercellular adhesion molecule- 1 (ICAM-1) and HLA-A, -B, -C, and -DR on highly purified primary human HC in cell culture. Expression was assessed by semiquantitative measurement of HC in cell culture by means of computer-aided fluorometry after immunofluorescent labeling. Avidinbiotin- immunoperoxidase staining was applied on parallel cultures to evaluate cell purity (〉99%) and to confirm the results obtained by fluorometry.ICAM-1 was expressed constitutively on untreated HC in vitro. Stimulation of HC with IFN-γ and IL-1α for 24 hr resulted in an increase of ICAM-1 expression. Cultured HC were moderately HLA-A, -B, and -C positive, but HLA-DR negative. Stimulation of HC with 500 U/ml IFN-g for 72 hr resulted in an increase of HLA-A, -B, -C, and -DR expression, whereas stimulation with 10 U/ml IL-1α for 72 hr had no influence. By using 5000 U/ml IFN-α for 72 hr, we achieved an increase of HLA-A, -B, and -C expression; effects on the other tested antigens were not significant. In contrast to endothelial cells and transformed human hepatocytic cell lines, ICAM-1 on HC was changed more intensively by IFN-γ than by IL-1α. Furthermore, the results reveal differences in HLA and ICAM-1 expression between HC in vivo and in vitro.
    Keywords: Inflammation ; Hepatocytes ; Histocompatibility Antigen HLA ; Man ; Cellular Components ; Cytokines ; Dr Determinant ; A Determinant ; B Determinant ; C Determinant ; Intercellular Adhesion Molecule 1 ; A Determinant ; B Determinant ; C Determinant ; Dr Determinant ; Cytokines ; Intercellular Adhesion Molecule 1;
    ISSN: 0041-1337
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  • 3
    Language: English
    In: Zeitschrift fur Ernahrungswissenschaft, March, 1993, Vol.32(1), p.56(11)
    Description: Byline: M. Sachs (1), F. Asskali (1,2), H. Forster (1,2), A. Encke (1) Keywords: Hereditare Fructoseintoleranz; Klinik; Stoffwechseleffekte; Hereditary fructose intolerance; clinical symptoms; metabolic changes Abstract (German): Wir berichten uber eine erwachsene Patientin mit zunachst nicht diagnostizierter hereditarer Fructoseintoleranz (HFI), die zweimal innerhalb von 2 Jahren nach elektiven Operationen und mehrmaliger perioperativer Gabe von Fructose und Sorbit ein aLeber- und Nierenversagen unklarer Genese" entwickelte und uberlebte. Wir konnten spater bei der Patientin und einem ihrer Bruder, bei beiden war seit ihrer fruhesten Kindheit eine Obst- und Sussspeisenunvertraglichkeit bekannt, durch einen Fructosetoleranztest die Diagnose einer HFI sichern. Zusatzlich wurden in der Literatur erwahnte Todesfalle nach parenteraler Gabe von Fructose und Sorbit ausgewertet. Wahrend der Fructoseinfusion konnten bei beiden Geschwistern mit HFI folgende Stoffwechselveranderungen nachgewiesen werden: Hypoglycamie, verstarkter Anstieg der Blutfructosekonzentration, Hyperlactatamie und Hyperammoniakamie. Diese Stoffwechselveranderungen waren nach Beendigung der Fructoseinfusion reversibel. Die Literaturauswertung ergab, dass bei den Todesfallen nach parenteraler Fructoseapplikation bei allen erfassten Patienten mit HFI eine Obst- bzw. Sussspeisenunvertraglichkeit bekannt war und dass offenbar keine regelmassigen Stoffwechselkontrollen durchgefuhrt wurden. Trotz eindeutiger Symptomatik wurde bei den in der Literatur erwahnten Todesfallen die Diagnose aHFI" nicht gestellt und damit die Entscheidung zum Abbruch der Infusionstherapie mit Zuckeraustauschstoffen nicht getroffen. Abstract: The present paper reports on an adult female patient whose hereditary fructose intolerance (HFI) was at first not diagnosed and who, within the space of 2 years after repeated elective surgery and the perioperative administration of fructose and sorbitol, developed "hepatic and renal failure of unclear origin." At a later stage we were able to establish the diagnosis of HFI by means of a fructose tolerance test in both she and her brother, for whom intolerance to fruit and desserts had been known since early childhood. In addition, literature references to fatalities following the parenteral application of fructose and sorbitol were analyzed. During the course of fructose infusion in both the patient and her brother with HFI, the following metabolic changes were noted: hypoglycemia, elevated rise in the blood fructose concentration, hyperlactacidemia, elevated rise in the blood fructose concentration, hyperlactacidemia, and hyperammonemia. These metabolic changes proved to be reversible after discontinuing the fructose infusion. Analysis of the literature on the fatalities following parenteral fructose administration established that fruit and dessert intolerance was known for all collated patients with HFI, and that, clearly, no regular metabolic tests had been conducted. Author Affiliation: (1) Klinik fur Allgemein- und Abdominalchirurgie, Germany (2) Abteilung fur Experimentelle Anaesthesiologie, Klinikum der Johann Wolfgang Goethe-Universitat, Frankfurt am Main, Germany Article History: Registration Date: 18/04/2005 Received Date: 15/06/1992 Accepted Date: 10/10/1992
    Keywords: Fructose
    ISSN: 0044-264X
    Source: Cengage Learning, Inc.
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  • 4
    Book chapter
    Book chapter
    Language: German
    In: Viszeralchirurgie, Chapter Kapitel 34, pp.669-704
    ISBN: 978-3-437-23710-2
    Source: ScienceDirect (Elsevier B.V.)
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  • 5
    In: Transplantation, 2000, Vol.69(9), pp.1977-1981
    Description: BACKGROUND.: Prostaglandin E2 (PGE2) is a powerful endogenous immune suppressant and interferes with various T-cell functions. However, it is not known in detail whether immunosuppressive drugs influence the PGE2-driven immune response in transplant patients. Therefore, we investigated the effect of several immunosuppressive compounds, in particular the novel drug mycophenolate mofetil (MMF), on endothelial PGE2 release. METHODS.: Endothelial cells (HUVEC) were activated by either allogeneic CD4 or CD8 T cells, or by the cytokines interleukin-1 or γ-interferon. Using an enzyme-linked immunosorbent assay, we analyzed PGE2 release of the activated HUVEC in the presence of MMF, cyclosporine, or tacrolimus. As verapamil and mibefradil also possess immunosuppressive properties, they were included in the study as well. RESULTS.: Activation of HUVEC with interleukin-1 or T cells resulted in a drastic accumulation of PGE2 in the supernatant. Cyclosporine or tacrolimus had no effect on PGE2 release. However, Ca channel blockers, when applied at higher dosages, caused a significant increase in PGE2. Interestingly, MMF strongly diminished the PGE2 level in the cell culture supernatant in a concentration-dependent manner. CONCLUSION.: The results demonstrate an inhibitory effect of MMF on PGE2 production, which may lower the benefits of the PGE2-triggered immune response after organ transplantation.
    Keywords: Endothelium ; Cytokines ; Lymphocytes T ; Immunosuppression ; Transplantation ; Interleukin 1 ; ^G-Interferon ; Prostaglandin E2 ; Mycophenolate Mofetil ; Clinical ; Man ; Immunology ; Gamma -Interferon ; Immunology ; Man ; Mycophenolate Mofetil ; Prostaglandin E2;
    ISSN: 0041-1337
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  • 6
    In: Transplantation, 2000, Vol.69(4), pp.588-597
    Description: BACKGROUND.: Cyclosporine A (CsA) and tacrolimus prevent proliferation but not transendothelial migration of alloreactive lymphocytes into donor organs. As a result, serious adverse effects, such as nephrotoxicity and neurotoxicity, have been observed under CsA/tacrolimus therapy. The incorporation of new drugs with infiltration blocking properties might enhance the efficacy of the current immunosuppressive protocol, allowing lower CsA/tacrolimus dosage. Because Ca plays a critical role in cell-cell interaction, the Ca-channel blocker verapamil might be a good cany.didate for supporting CsA/tacrolimus-based therap METHODS.: A T-cell endothelial cell coculture model or immobilized immunoglobulin G globulin chimeras were employed to investigate how S- and R- verapamil interfere with the lymphocytic infiltration process. The expression and arrangement of membranous adhesion receptors and cytoskeletal F-actin filaments were analyzed by fluorometric method in the presence of. verapamil. RESULTS.: Both verapamil enantiomers strongly inhibited lymphocyte infiltration. CD4 and CD8 T-cells were influenced to a similar extent with regard to horizontal locomotion (CD4=CD8), but to a different extent with regard to adhesion and penetration (CD4 〉 CD8). Moreover, penetration was blocked to a higher extent than was adhesion. ID50-values were 31 μM (CD4-adhesion) and 11 μM (CD4-penetration). Verapamil reduced P-selectin expression on endothelial cells and effectively down-regulated binding of T-cells to immobilized P-selectin immunoglobulin G globulins (ID50=4.4 μM; CD4). A verapamil-induced reduction of intracellular F-actin in T-lymphocytes was proven to be mainly responsible for diminished cell locomotion. CONCLUSIONS.: The prevention of CD4 T-cell penetration by verapamil might argue for its use as an adjunct to CsA/tacrolimus-based immunosuppressive therapy.
    Keywords: Immunosuppression ; Endothelium ; Lymphocytes T ; Immunosuppressive Agents ; Cell Motility ; Verapamil ; Calcium Channel Blockers ; Experimental ; Function ; Immunology ; Calcium Channel Blockers ; Cell Motility ; Immunology ; Verapamil;
    ISSN: 0041-1337
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  • 7
    Language: German
    In: Astronomische Nachrichten, 1846, Vol.24(19), pp.277-292
    ISSN: 0004-6337
    E-ISSN: 1521-3994
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  • 8
    Language: German
    In: Zentralblatt für Chirurgie, 2001, Vol.126(11), pp.861-861
    ISSN: 0044-409X
    E-ISSN: 1438-9592
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  • 9
    Language: German
    In: Rontgen-Blatter; Zeitschrift fur Rontgen-Technik und medizinisch-wissenschaftliche Photographie, December 1972, Vol.25(12), pp.557-61
    Keywords: Angiography -- Instrumentation
    ISSN: 0300-8592
    Source: MEDLINE/PubMed (U.S. National Library of Medicine)
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  • 10
    Language: English
    In: International Journal of Colorectal Disease, 2007, Vol.22(2), pp.215-223
    Description: Byline: E. Ryschich (1), G. Huszty (1), N. Wentzensen (2), E. Schmidt (1), H. P. Knaebel (1), J. Encke (3), A. Marten (1), M. W. Buchler (1), J. Schmidt (1) Keywords: Pancreatic cancer; Experimental therapy; Flt3 ligand Abstract: Background Fms-like tyrosine kinase 3 receptor (Flt3) is an important receptor expressed on the cell membrane of immature antigen-presenting cells. The binding of Flt3 to its ligand (FL) activates the proliferation of dendritic cells (DCs). This mechanism is currently being evaluated in the therapy of malignant tumors. The aim of the present study was to study the effect of FL gene transfer on the immune response and tumor growth in experimental pancreatic cancer. Materials and methods The rat FL was sequenced and cloned from total mRNA extract of the spleen. Transfection efficiency of subcutaneously growing rat duct-like pancreatic cancer (DSL6A) with DOTAP-/cholesterol-based liposomes was tested using a pcDNA3.1-lacZ construct. Flt3 ligand production of in vitro transfected tumor cells and in vivo transfected tumors was measured by enzyme-linked immunosorbent assay. Tumor induction was achieved in Lewis rats by a subcutaneous inoculation of syngeneic pancreatic tumor cells (DSL6A). The animals were allocated into three groups: control, mock treatment, and treatment with FL plasmid. The plasmid was injected intratumorally three times per week for 2 weeks. The total observation time was 6 weeks. Results The tumor volume was significantly lower in the FL-transfected group during the first 3 weeks. The number of responders was significantly higher in the FL group compared with control and mock treatment. The number of CD80+ DCs in the spleen was significantly higher after FL gene transfer. The responders showed a significantly higher number of splenic natural killer (NK) cells. There were no differences of infiltrating lymphocytes, proliferation, and tumor blood vessels between the groups. Conclusion Intratumoral gene transfer of FL in rats activated proliferation of DCs and NK cells, which causes a moderate reduction of tumor growth. This improvement of local tumor control during the first weeks could be explained by an improved antigen presentation. Author Affiliation: (1) Dept. of Surgery, University of Heidelberg, Im Neuenheimer Feld 110, 69120, Heidelberg, Germany (2) Dept. of Molecular Pathology, University of Heidelberg, Heidelberg, Germany (3) Dept. of Gastroenterology, University of Heidelberg, Heidelberg, Germany Article History: Registration Date: 06/02/2006 Accepted Date: 03/02/2006 Online Date: 10/03/2006 Article note: The first two authors contributed equally to the work.
    Keywords: Pancreatic cancer ; Experimental therapy ; Flt3 ligand
    ISSN: 0179-1958
    E-ISSN: 1432-1262
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