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  • 1
    Language: English
    In: Der Pathologe, Sept, 2013, Vol.34(5), p.429(7)
    Description: Byline: A. Erbersdobler (1) Keywords: Morphologische Erscheinungsformen; Atrophie; Hyperplasie; Metaplasie; Entzundung; Morphologic appearance; Atrophy; Hyperplasia; Metaplasia; Inflammation Abstract (German): Zu den nichtneoplastischen Veranderungen der Prostata gehoren entzundliche, atrophische, hyper- und metaplastische Reaktionsmuster des Drusenepithels und des fibromuskularen Stromas. Ausserdem sind im Biopsiematerial gelegentlich histoanatomische Strukturen von ausserhalb des Prostatadrusengewebes enthalten. Die Kenntnis der morphologischen Erscheinungsformen benigner reaktiver Lasionen ist zur differenzialdiagnostischen Abgrenzung gegenuber malignen Prozessen wichtig. Das Wissen um die genaue Lokalisation der Gewebeentnahme und immunhistologische Zusatzuntersuchungen sind hierzu oft hilfreich oder erforderlich. Abstract: Non-neoplastic changes in the prostatic gland include inflammatory, atrophic, hyperplastic and metaplastic reaction patterns of the glandular epithelium and the fibromuscular stroma. Furthermore, histoanatomical structures from outside the prostatic gland are sometimes included in biopsy material. Knowledge of the morphological appearance of benign, reactive lesions is important in order to differentiate them from malignancies. To this aim knowing the precise location of tissue sampling as well as ancillary immunohistochemical investigations are often useful or necessary. Author Affiliation: (1) Institut fur Pathologie, Universitatsmedizin Rostock, Strempelstr. 14, 18055, Rostock, Deutschland Article History: Registration Date: 15/05/2013 Online Date: 25/07/2013
    ISSN: 0172-8113
    Source: Cengage Learning, Inc.
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  • 2
    In: PLoS ONE, 2015, Vol.10(8)
    Description: University and museum collections are very important sources of biological samples that can be used to asses the past and present genetic diversity of many species. Modern genetic and immunohistochemical techniques can be used on long-term preserved fixed tissues from museum specimens to answer epidemiological questions. A proof of principle was established to apply modern molecular genetics and immunohistochemical methods to these old specimens and to verify the original diagnosis. We analysed 19 specimens from our university collection including human organs that had been in fixative for more than 80 years. The tissues originated from lung, colon, brain, heart, adrenal gland, uterus and skin. We isolated amplifiable DNA from these wet preparations and performed mutational analysis of BRAF, KRAS and EGFR. The tissues were also embedded in paraffin and used for modern histology and immunohistochemistry. Our data show that amplifiable DNA is extractable and ranged from 0.25 to 22.77 μg of total DNA. In three specimens BRAF V600E or KRAS G12D mutations were found. Additionally, expression of different proteins like vimentin and GFAP was detected immunohistochemical in six investigated specimens. On the basis of our results the original diagnosis was altered in three specimens. Our work showed that it is possible to extract amplifiable DNA suitable for sequence analysis from long-term fixed tissue. Furthermore, histology and immunohistochemistry is feasible in specimens fixed long time ago. We conclude that these old preparations are suitable for further epidemiological research and that our methods open up new opportunities for future studies.
    Keywords: Research Article
    E-ISSN: 1932-6203
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  • 3
    Language: English
    In: Oncology Reports, February 2012, Vol.27(2), pp.389-395
    Description: Solid tumours need to induce their own vascular supply, and microvessel density (MVD) has emerged as a prognostic factor in several tumours. We hypothesized that mRNA levels of some endothelial factors in prostate cancer tissue would correlate with histologically measured MVD, or other pathological parameters. Expression levels of the endothelial factors CD31, CD34, CD105, CD144, CD146, CAV1 and VEGFR2 were assessed by RT-qPCR in matched freshly frozen normal and tumour tissues from 69 patients that underwent radical prostatectomy. The results were compared to pathological parameters and the MVD in the corresponding paraffin-embedded material, as determined by immunohistochemistry against CD31 and CD34. Comparing mRNA expression in matched normal and tumour samples, only CAV1 showed relevant differences, being down-regulated in tumour tissues (fold change = -1.89, P〈0.0001). CAV1 down-regulation correlated with pT category (P=0.006) and the Gleason score (P=0.041). In a univariate analysis, lower CAV1 mRNA expression was associated with biochemical recurrence (P=0.019). By immunohistochemistry, CAV1 was mainly localized in endothelial and stromal cells and showed a weaker staining pattern in the tumour compared to normal tissue. Furthermore, MVD significantly correlated with tumour grade and pT category. There was no significant association between endothelial mRNA expression and histologically determined MVD in tumour tissues, but only a trend for CD31 mRNA (P=0.074) and an inverse trend for CAV1 mRNA (P=0.056). In conclusion, there is only a weak correlation between the mRNA expression of endothelial factors and MVD in prostatic tumour tissue. However, loss of CAV1 mRNA expression may play a role in prostate cancer progression.
    Keywords: Surgical Stapler;
    ISSN: 1021-335X
    E-ISSN: 17912431
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  • 4
    Language: English
    In: The Journal of nutrition, September 2012, Vol.142(9), pp.1638-44
    Description: We aimed to investigate the effects of increased intake of α-linolenic acid (ALA), EPA, or DHA incorporated into a food matrix on the fatty acid composition of erythrocytes and on biomarkers of oxidant/antioxidant status. To this end, a controlled dietary study was conducted in 74 healthy men and women. The participants were randomly assigned to 1 of 3 interventions in which margarines fortified with either 10 weight percent ALA, EPA, or DHA ethyl esters replaced their normal spread for 6 wk. The total intakes of ALA, EPA, and DHA were 4.4, 2.2, and 2.3 g/d, respectively. Consuming EPA increased the erythrocyte proportion of EPA (394%) and the omega-3 index (sum of EPA and DHA, 38%). Consumption of DHA increased erythrocyte DHA (91%), the omega-3 index (98%), and EPA (137%). The omega-3 index increased to a significantly greater extent in the DHA group than in the EPA group. ALA did not increase erythrocyte EPA or the omega-3 index. We found no change in plasma uric acid or antioxidant capacity in any of the groups. Plasma malondialdehyde (MDA) increased with the EPA and DHA interventions. All 3 interventions decreased erythrocyte linoleic acid hydroperoxides but did not affect their MDA concentrations. In conclusion, the intake of both isolated EPA and DHA incorporated into margarine resulted in an enhanced incorporation of EPA and DHA into erythrocytes. Our findings indicate that DHA is quantitatively superior to EPA in view of the EPA+DHA tissue incorporation and also that 4 g/d ALA is not sufficient to increase the omega-3 index over a 6-wk period.
    Keywords: Food, Fortified ; Margarine ; Antioxidants -- Metabolism ; Erythrocytes -- Drug Effects ; Fatty Acids, Omega-3 -- Administration & Dosage
    ISSN: 00223166
    E-ISSN: 1541-6100
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  • 5
    Language: English
    In: Clinical Genitourinary Cancer, April 2017, Vol.15(2), pp.192-195
    Description: Carcinoma of the penis is a rare tumor in the United States and in western European countries. Clinical management has become more complex in recent years, because organ-preserving strategies are being favored. Furthermore, our understanding of the pathogenesis of this malignancy has grown considerably. As a result of these developments, the demands on the pathology reports of surgical specimens from the penis have increased. There are also some peculiarities with the current World Health Organization and TNM classification systems of penile cancer as compared with other tumor entities. This review outlines the most relevant aspects that have to be considered in the pathologic handling and typing of penile carcinoma.
    Keywords: Classification ; Histology ; Pathology ; Penile Cancer ; Prognosis ; Medicine
    ISSN: 1558-7673
    E-ISSN: 1938-0682
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  • 6
    Language: German
    In: Der Urologe, 2018, Vol.57(4), pp.391-397
    Description: To access, purchase, authenticate, or subscribe to the full-text of this article, please visit this link: http://dx.doi.org/10.1007/s00120-018-0592-8 Byline: A. Erbersdobler (1) Keywords: Humane Papillomaviren; Neoplasie; Vorlauferlasion; Malignom; Plattenepithelkarzinom; Human papillomavirus; Neoplasms; Precancerous conditions; Malignancy; Squamous cell carcinoma Abstract (German): Hintergrund Das Peniskarzinom ist in Deutschland und in den westlichen Industrienationen ein seltener Tumor. Das Wissen uber die Pathogenese und die Pathologie dieses Malignoms hat in den letzten Jahren erheblich zugenommen. Fragestellung Das klinische Management ist komplexer geworden und organerhaltende Therapiestrategien werden heute bevorzugt. Damit steigt auch die Anforderung an die pathohistologische Begutachtung von Biopsien und Operationspraparaten des Penis. Material und Methoden Diese Ubersichtsarbeit erlautert auf der Grundlage der Leitlinien und der relevanten Literatur die wichtigsten Aspekte, die bei der Klassifikation und bei der pathohistologischen Befunderstellung des Peniskarzinoms berucksichtigt werden mussen. Ergebnisse Die korrekte histologische Subtypisierung des Peniskarzinoms ist wichtig fur die Prognose und die Therapieentscheidung. Es gibt auch Besonderheiten in der aktuellen TNM-Klassifikation ("tumour, node, metastasis") dieses Tumors im Vergleich zu anderen Entitaten. Schlussfolgerungen Die Bearbeitung der Gewebepraparate und die histopathologische Typisierung erfordern vom Pathologen Kenntnisse der aktuellen Entwicklungen zur Pathogenese, Klassifikation und Therapie des Peniskarzinoms. Abstract: Background Penile cancer is rare in Germany and in western European countries. Our understanding of the pathogenesis and pathology of this malignancy has increased considerably in recent years. Objectives Clinical management has become more complex, with organ-preserving strategies being increasingly favored. Associated with these developments, the demands on the pathology reports of biopsies and surgical specimens from the penis have also increased. Materials and methods According to guidelines and the relevant literature, this review outlines the most important aspects that must be considered in the classification and pathological reporting of penile cancer. Results Correct histological subtyping of penile cancer is important for prognostic and therapeutic considerations. There are also some peculiarities with the current TNM classification system of this tumor compared to other entities. Conclusion Handling of specimens and histopathological typing must be performed by experienced pathologists according to recent developments in the pathogenesis, classification, and therapeutic strategies of penile cancer. Author Affiliation: (1) 0000 0000 9737 0454, grid.413108.f, Institut fur Pathologie, Universitatsmedizin Rostock, Strempelstra[sz]e 14, 18057, Rostock, Deutschland Article History: Registration Date: 31/01/2018 Online Date: 21/02/2018
    Keywords: Human papillomavirus ; Neoplasms ; Precancerous conditions ; Malignancy ; Squamous cell carcinoma
    ISSN: 0340-2592
    E-ISSN: 1433-0563
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  • 7
    Language: German
    In: Der Pathologe, 2013, Vol.34(5), pp.429-435
    Description: Zu den nichtneoplastischen Veränderungen der Prostata gehören entzündliche, atrophische, hyper- und metaplastische Reaktionsmuster des Drüsenepithels und des fibromuskulären Stromas. Außerdem sind im Biopsiematerial gelegentlich histoanatomische Strukturen von außerhalb des Prostatadrüsengewebes enthalten. Die Kenntnis der morphologischen Erscheinungsformen benigner reaktiver Läsionen ist zur differenzialdiagnostischen Abgrenzung gegenüber malignen Prozessen wichtig. Das Wissen um die genaue Lokalisation der Gewebeentnahme und immunhistologische Zusatzuntersuchungen sind hierzu oft hilfreich oder erforderlich. Non-neoplastic changes in the prostatic gland include inflammatory, atrophic, hyperplastic and metaplastic reaction patterns of the glandular epithelium and the fibromuscular stroma. Furthermore, histoanatomical structures from outside the prostatic gland are sometimes included in biopsy material. Knowledge of the morphological appearance of benign, reactive lesions is important in order to differentiate them from malignancies. To this aim knowing the precise location of tissue sampling as well as ancillary immunohistochemical investigations are often useful or necessary.
    Keywords: Morphologic appearance ; Atrophy ; Hyperplasia ; Metaplasia ; Inflammation
    ISSN: 0172-8113
    E-ISSN: 1432-1963
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  • 8
    Language: English
    In: PLoS ONE, 01 January 2017, Vol.12(1), p.e0170353
    Description: University anatomical-pathological collections represent huge sources of human tissues and preparations from a variety of different diseases. With the help of modern genetic and histological methods, preserved fixed tissues from pathological collections can be used to re-evaluate former diagnoses. We analysed 25 specimens from our pathological collection with ages ranging from 78 to 112 years. The tissues originated from the oral cavity, lip, tongue, lung, bone, kidney, spleen, thymus, larynx, lymph node, penis and uterine cervix with an original diagnosis of epithelial cancers or tuberculosis. Amplifiable DNA was extracted and in epithelial cancers, potential HPV infection was investigated. Specimens with an original diagnosis of tuberculosis were examined for mycobacterial infection. The tissues were also examined using modern histological methods. Our data showed that in 24/25 specimens the histological structure was preserved and in 10/11 specimens the diagnosis of squamous cell carcinoma could be confirmed. Additionally, HPV type 16 was detected in 8 specimens. The histological pattern of tuberculosis was found in 11/14 specimens and the Mycobacterium tuberculosis complex was ascertained in four specimens. Our study showed that pathogens such as HPV or Mycobacterium tuberculosis can be detected in historical pathological preparations, and that these collections are suitable for further epidemiological research.
    Keywords: Sciences (General)
    E-ISSN: 1932-6203
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  • 9
    Language: English
    In: The Journal of Urology, March 2012, Vol.187(3), pp.899-907
    Description: We assessed the prognostic role of p16 expression in penile cancer with respect to cancer specific survival. Based on a multi-institutional collaboration wax embedded tissues from 92 surgically treated patients, including 27 with total and 65 with partial penectomy, were retrospectively evaluated. After a central histopathological review by 1 pathologist a tissue microarray was constructed for p16 immunostaining. Two independent pathologists evaluated p16 expression, which was correlated with cancer specific survival. The κ statistic was used to assess interobserver variability. Univariate and multivariate Cox proportional hazards analysis was applied to assess the independent effects of prognostic factors on cancer specific survival during a median postoperative followup of 32 months (IQR 6–66). The κ statistic revealed excellent interobserver agreement (κ 0.934, p 〈0.001). Two and 5-year cancer specific survival rates for the entire study cohort were 86% and 74%, respectively. The 2 and 5-year rates for patients without and with p16 expression differed significantly (73% and 57% vs 95% and 85%, respectively, p = 0.011). Univariate analysis revealed p16 expression as a significant prognostic factor with respect to cancer specific survival (p = 0.018). Multivariate analysis identified koilocytosis (HR 0.24, 95% CI 0.07–0.83, p = 0.024), p16 expression (HR 0.44, 95% CI 0.23–0.84, p = 0.013), and histological stage (HR 3.54, 95% CI 1.88–6.67, p 〈0.001) and grade (HR 2.47, 95% CI 1.00–6.09, p = 0.049) as independent prognostic factors for cancer specific survival. Results show that p16 seems to be a prognostic parameter for primary invasive penile cancer with excellent interobserver reproducibility. At pathology laboratories without antibodies against p16 conventional histological determination of koilocytosis by the pathologist also appears to provide important prognostic information for cancer specific survival.
    Keywords: Penis ; Carcinoma ; Squamous Cell ; Cyclin-Dependent Kinase Inhibitor P16 ; Prognosis ; Mortality ; Medicine
    ISSN: 0022-5347
    E-ISSN: 1527-3792
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  • 10
    Language: English
    In: The Journal of Urology, April 2013, Vol.189(4), pp.e390-e391
    Keywords: Medicine
    ISSN: 0022-5347
    E-ISSN: 1527-3792
    Source: ScienceDirect Journals (Elsevier)
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