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  • 1
    Language: English
    In: Proceedings of the National Academy of Sciences of the United States of America, 25 August 2015, Vol.112(34), pp.E4772-81
    Description: Horizontal gene transfer via plasmid conjugation is a major driving force in microbial evolution but constitutes a complex process that requires synchronization with the physiological state of the host bacteria. Although several host transcription factors are known to regulate plasmid-borne transfer genes, RNA-based regulatory circuits for host-plasmid communication remain unknown. We describe a posttranscriptional mechanism whereby the Hfq-dependent small RNA, RprA, inhibits transfer of pSLT, the virulence plasmid of Salmonella enterica. RprA employs two separate seed-pairing domains to activate the mRNAs of both the sigma-factor σ(S) and the RicI protein, a previously uncharacterized membrane protein here shown to inhibit conjugation. Transcription of ricI requires σ(S) and, together, RprA and σ(S) orchestrate a coherent feedforward loop with AND-gate logic to tightly control the activation of RicI synthesis. RicI interacts with the conjugation apparatus protein TraV and limits plasmid transfer under membrane-damaging conditions. To our knowledge, this study reports the first small RNA-controlled feedforward loop relying on posttranscriptional activation of two independent targets and an unexpected role of the conserved RprA small RNA in controlling extrachromosomal DNA transfer.
    Keywords: Hfq ; Rpra ; Feedforward Control ; Plasmid Conjugation ; Srna ; Chromosomes, Bacterial ; DNA, Bacterial -- Genetics ; RNA, Bacterial -- Genetics ; Salmonella -- Genetics
    ISSN: 00278424
    E-ISSN: 1091-6490
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  • 2
    In: Molecular Microbiology, March 2014, Vol.91(6), pp.1057-1069
    Description: is a quiescent ‐type regulator belonging to the ‐ regulon. Activation of transcription represses expression of pathogenicity island 1 (‐1) in serovar yphimurium and inhibits invasion of epithelial cells. Loss of suppresses ‐mediated downregulation of ‐1. Activation of transcription reduces the level of protein, and loss of restores the wild type level. Hence, ‐mediated downregulation of ‐1 may involve inhibition of activity by , a view consistent with the fact that is a inhibitor. analyses using β‐galactosidase fusions indicate that activates transcription. analyses by slot blotting, electrophoretic mobility shift analysis and footprinting show that binds the promoter region. Although residual ‐1 repression by is observed in the absence of , the ‐‐ ‘pathway’ appears to be the major mechanism. Because both and ‐1 are repressed by ‐, activation of transcription may provide a backup mechanism for ‐1 repression under conditions that impair ‐‐mediated silencing.
    Keywords: Salmonella -- Analysis;
    ISSN: 0950-382X
    E-ISSN: 1365-2958
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  • 3
    Language: English
    In: Genetics, April 2010, Vol.184(4), pp.947-58
    Description: The virulence plasmid of Salmonella enterica (pSLT) is an F-like conjugative plasmid. High rates of pSLT transfer occur in the mammalian gut, a microaerobic environment. In this study, we describe genetic screens for host-encoded activators and repressors of the transfer operon (tra) of pSLT. We show that the transcription factor ArcA is an activator of conjugation, especially under microaerobiosis. In turn, succinate dehydrogenase (SdhABCD) is a repressor of mating in aerobiosis. ArcA binds upstream of the main tra promoter (p(traY)) and activates tra transcription, as previously described in F, R1, and R100. In the absence of ArcA, transfer of pSLT decreased 7-fold in aerobiosis and 〉100-fold in microaerobiosis. In aerobiosis, ArcA activates the traY promoter in an ArcB-independent manner, as described in other F-like plasmids. In microaerobiosis, however, the ArcB sensor is necessary for activation of p(traY). Lack of Sdh causes a 〉20-fold increase in pSLT transfer in aerobiosis, but has little effect under microaerobiosis. Sdh inhibits conjugal transfer by reducing traJ transcription, probably in an indirect manner. In turn, the sdhCDAB operon is repressed by the ArcAB system under microaerobiosis. Hence, the ArcAB two-component system of S. enterica stimulates pSLT transfer under microaerobiosis by two concerted actions: activation of the tra operon and repression of the sdhCDAB operon.
    Keywords: Aerobiosis ; Conjugation, Genetic ; Bacterial Proteins -- Metabolism ; Salmonella Enterica -- Genetics ; Succinate Dehydrogenase -- Metabolism ; Transcription Factors -- Metabolism
    ISSN: 00166731
    E-ISSN: 1943-2631
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  • 4
    Language: English
    In: Molecular Microbiology, Sept, 2012, Vol.85, p.1072(18)
    Keywords: Transcription (Genetics) -- Genetic Aspects ; Transcription (Genetics) -- Analysis ; Bacterial Genetics -- Genetic Aspects ; Bacterial Genetics -- Analysis ; Preventive Medicine -- Analysis ; Dna Binding Proteins -- Genetic Aspects ; Dna Binding Proteins -- Analysis ; Genes -- Genetic Aspects ; Genes -- Analysis ; Rna -- Genetic Aspects ; Rna -- Analysis ; Salmonella -- Analysis
    ISSN: 0950-382X
    Source: Cengage Learning, Inc.
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  • 5
    Language: English
    In: Molecular Microbiology, Sept, 2012, Vol.85, p.1072(18)
    Description: To purchase or authenticate to the full-text of this article, please visit this link: http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2012.08162.x/abstract Byline: Shane C. Dillon(1), Elena Espinosa(3), Karsten Hokamp(2), David W. Ussery(4), Josep Casadesus(3), Charles J. Dorman(1) Summary We report the first investigation of the binding of the Salmonella enterica LeuO LysR-type transcription regulator to its genomic targets in vivo. Chromatin-immunoprecipitation-on-chip identified 178 LeuO binding sites on the chromosome of S. enterica serovar Typhimurium strain SL1344. These sites were distributed across both the core and the horizontally acquired genome, and included housekeeping genes and genes known to contribute to virulence. Sixty-eight LeuO targets were co-bound by the global repressor protein, H-NS. Thus, while LeuO may function as an H-NS antagonist, these functions are unlikely to involve displacement of H-NS. RNA polymerase bound 173 of the 178 LeuO targets, consistent with LeuO being a transcription regulator. Thus, LeuO targets two classes of genes, those that are bound by H-NS and those that are not bound by H-NS. LeuO binding site analysis revealed a logo conforming to the TN.sub.11A motif common to LysR-type transcription factors. It differed in some details from a motif that we composed for Escherichia coli LeuO binding sites; 1263 and 1094 LeuO binding site locations were predicted in the S. Typhimurium SL1344 and E. coli MG1655 genomes respectively. Despite differences in motif composition, many LeuO target genes were common to both species. Thus, LeuO is likely to be a more important global regulator than previously suspected. Author Affiliation: (1)Department of Microbiology, School of Genetics and Microbiology, Moyne Institute of Preventive Medicine (2)Department of Genetics, School of Genetics and Microbiology, Smurfit Institute, Trinity College Dublin, Dublin 2, Ireland (3)Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Apartado 1095, Seville 41080, Spain (4)Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark Correspondence: (*) E-mail cjdorman@tcd.ie; Tel. (+353) 1 8962013; Fax (+353) 1 6799294. Accepted 29 June, 2012. Supporting information: Additional Supporting Information may be found in the online version of this article
    Keywords: Transcription (Genetics) -- Genetic Aspects ; Transcription (Genetics) -- Analysis ; Bacterial Genetics -- Genetic Aspects ; Bacterial Genetics -- Analysis ; Preventive Medicine -- Analysis ; Dna Binding Proteins -- Genetic Aspects ; Dna Binding Proteins -- Analysis ; Genes -- Genetic Aspects ; Genes -- Analysis ; Rna -- Genetic Aspects ; Rna -- Analysis ; Salmonella -- Analysis
    ISSN: 0950-382X
    Source: Cengage Learning, Inc.
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  • 6
    In: International journal of obesity (2005), 2018, Vol.42(3), p.302-309
    Description: Background/Objectives The clinical relevance of the metabolically healthy overweight/obese (MHO) phenotype is controversial and the relationships between weight change and the development of cardiometabolic risk factors is unknown. Therefore, we aim to: 1) Assess the long-term risk of developing one or more components of the metabolic syndrome in MHO adults compared with metabolically healthy normal weight (MHNW); 2) Evaluate risk of a composite of death, CVD, and risk of developing type 2 diabetes between adults defined according to baseline BMI and metabolic health. Subjects/Methods Retrospective cohort study of adults 18–65 years of age seen at our institution between 1998 and 2000 who lived in Olmsted County. Metabolically healthy was defined as the absence of all components of the metabolic syndrome (except for waist circumference). Main outcome was the development of metabolic risk factors. The secondary outcome was a composite of mortality, CVD and heart failure. Results Of the 18071 individuals with complete data at baseline, 1805 (10%) were MHO (mean age 38 ± 11 years) and 3048 were MHNW (mean age 35 ± 11 years). After a median follow up of 15 years, IQR 10–17, 80% of MHO vs. 68% of MHNW developed at least one cardiometabolic risk factor ( p 〈 0.001). In multivariate analysis, MHO individuals who gained ≥ 10% of their body weight were more likely to have developed metabolic complications compared to MHO individuals that did not gain weight ( p =0.001 for 10–15%, p 〈 0.001 for 〉 15% weight gain). The risk for the secondary composite endpoint was similar between MHO and MHNW, number of events 218/1805 vs. 217/3048 for MHO and MHNW, respectively, (HR: 1.16, 95% CI 0.96–1.40). Conclusions MHO are more likely to develop metabolic complications than MHNW, especially if they gain weight.
    Keywords: Article ; Obesity ; Weight Gain ; Metabolic Health ; Cardiovascular Disease ; Diabetes
    ISSN: 0307-0565
    E-ISSN: 1476-5497
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  • 7
    In: Molecular Microbiology, September 2012, Vol.85(6), pp.1072-1089
    Description: We report the first investigation of the binding of the LeuO LysR‐type transcription regulator to its genomic targets . Chromatin‐immunoprecipitation‐on‐chip identified 178 LeuO binding sites on the chromosome of serovar Typhimurium strain SL1344. These sites were distributed across both the core and the horizontally acquired genome, and included housekeeping genes and genes known to contribute to virulence. Sixty‐eight LeuO targets were co‐bound by the global repressor protein, H‐NS. Thus, while LeuO may function as an H‐NS antagonist, these functions are unlikely to involve displacement of H‐NS. RNA polymerase bound 173 of the 178 LeuO targets, consistent with LeuO being a transcription regulator. Thus, LeuO targets two classes of genes, those that are bound by H‐NS and those that are not bound by H‐NS. LeuO binding site analysis revealed a logo conforming to the TNA motif common to LysR‐type transcription factors. It differed in some details from a motif that we composed for LeuO binding sites; 1263 and 1094 LeuO binding site locations were predicted in the . Typhimurium SL1344 and MG1655 genomes respectively. Despite differences in motif composition, many LeuO target genes were common to both species. Thus, LeuO is likely to be a more important global regulator than previously suspected.
    Keywords: Salmonella ; Genes ; Binding Sites ; Chromosomes ; Gene Expression ; Genomes;
    ISSN: 0950-382X
    E-ISSN: 1365-2958
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  • 8
    Language: Spanish
    In: Roca: Revista Científico - Educaciones de la provincia de Granma, 2017, Vol.13(4), pp.141-154
    Description: The work that is presented is been of a doctorate thesis and an investigation project. The student of the specialty Accounting needs to develop professional abilities to solve the problems of the countable practice. The diagnosis, throws difficulties that bear to outline as problem, the inadequacies that are presented in the professional formation of the students, specialty Accounting that you/they limit the solution of professional problems. The objective that pursues this investigation is the elaboration of a didactic strategy sustained in a didactic model for the development of the professional ability to Register economic facts. The didactic pattern is contentions of the problematización, construction and generalization of the development of the professional ability to Register economic facts. The strategy is composed by stages, planning, implementation and control, each one integrated by actions. Investigation methods were used, theoretical, empiric and statistical-mathematical. The application in the pedagogic practice reveals its effectiveness.  
    Description: El trabajo que se presenta es resultado de una tesis de doctorado y un proyecto de investigación. El estudiante de la especialidad Contabilidad necesita desarrollar habilidades profesionales para resolver los problemas de la práctica contable. El diagnóstico realizado, arroja dificultades que conllevan a plantear como problema, las insuficiencias que se presentan en la formación profesional de los estudiantes, especialidad Contabilidad, que limitan la solución de problemas profesionales. El objetivo que persigue esta investigación es la elaboración de una estrategia didáctica sustentada en un modelo didáctico para el desarrollo de la habilidad profesional Registrar hechos económicos en la asignatura Contabilidad. El modelo didáctico es contentivo de la problematización, construcción y generalización del desarrollo de la habilidad profesional Registrar hechos económicos. La estrategia está compuesta por etapas, planificación, implementación y control, cada una integrada por acciones. La aplicación en la práctica pedagógica revela su efectividad.
    ISSN: 2074-0735
    Source: Fundación Dialnet
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  • 9
    In: The Journal of Trauma: Injury, Infection, and Critical Care, 2010, Vol.69(5), pp.1282-1287
    Description: BACKGROUND:: The Surviving Sepsis Campaign was launched in 2002, aiming at a 25% reduction in mortality in sepsis during a 5-year period. We hypothesized that the compliance with an adapted sepsis bundle would improve intensive care unit (ICU) survival in a cohort of surgical septic shock patients. METHODS:: A retrospective, observational study was performed in surgical ICUs from two University hospitals. Seven quality indicators were considered to study the compliance with the sepsis bundle in 182 patients: (1) administration of antibiotics within 6 hours from diagnosis of septic shock, (2) initial effective antibiotic treatment, (3) adequate resuscitation within 6 hours after the diagnosis of septic shock, (4) administration of steroids, (5) use of activated protein C, (6) glucose control, and (7) protective ventilation. Univariate and multivariate logistic regression analyses were performed to make a predictive model to study the probability of survival according to the number of therapeutic guidelines fulfilled and to adjust for other predictive factors. RESULTS:: Compliance with individual guidelines was considered adequate in more than 60% of the cases, except in the case of glucose control. For all quality indicators, ICU survival was higher in the bundle-compliant patients. Survival (61%) was associated with the fulfilment of increasing number of therapeutic guidelines (odds ratio, 1.64; 95% confidence interval, 1.28–2.1; p 〈 0.001). CONCLUSIONS:: In surgical septic shock patients, the outcome was significantly related to the number of fulfilled therapeutic guidelines included in a sepsis bundle.
    Keywords: Mortality ; Activated Protein C ; Ventilation ; Glucose ; Survival ; Antibiotics ; Septic Shock ; Steroid Hormones ; Trauma ; Models ; Sepsis ; Intensive Care Units ; Regression Analysis ; Hospitals ; Human Diseases;
    ISSN: 0022-5282
    E-ISSN: 15298809
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  • 10
    Language: Spanish
    In: Ería: Revista cuatrimestral de geografía, 2013, Issue 92, pp.321-322
    ISSN: 0211-0563
    Source: Fundación Dialnet
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