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  • 1
    Language: English
    In: Journal of Bacteriology, 2012, Vol. 194(10), p.2646
    Description: Escherichia coli K-12 WcaJ and the Caulobacter crescentus HfsE, PssY, and PssZ enzymes are predicted to initiate the synthesis of colanic acid (CA) capsule and holdfast polysaccharide, respectively. These proteins belong to a prokaryotic family of membrane enzymes that catalyze the formation of a phosphoanhydride bond joining a hexose-1-phosphate with undecaprenyl phosphate (Und-P). In this study, in vivo complementation assays of an E. coli K-12 wcaJ mutant demonstrated that WcaJ and PssY can complement CA synthesis. Furthermore, WcaJ can restore holdfast production in C. crescentus. In vitro transferase assays demonstrated that both WcaJ and PssY utilize UDP-glucose but not UDP-galactose. However, in a strain of Salmonella enterica serovar Typhimurium deficient in the WbaP O antigen initiating galactosyltransferase, complementation with WcaJ or PssY resulted in O-antigen production. Gas chromatography-mass spectrometry (GC-MS) analysis of the lipopolysaccharide (LPS) revealed the attachment of both CA and O-antigen molecules to lipid A-core oligosaccharide (OS). Therefore, while UDP-glucose is the preferred substrate of WcaJ and PssY, these enzymes can also utilize UDP-galactose. This unexpected feature of WcaJ and PssY may help to map specific residues responsible for the nucleotide diphosphate specificity of these or similar enzymes. Also, the reconstitution of O-antigen synthesis in Salmonella, CA capsule synthesis in E. coli, and holdfast synthesis provide biological assays of high sensitivity to examine the sugar-1-phosphate transferase specificity of heterologous proteins. ; p. 2646-2657.
    Keywords: Gas-Chromatography-Mass Spectrometry ; Oligosaccharides ; Bioassays ; Glucose 1-Phosphate ; Lipopolysaccharides ; Salmonella Enterica Subsp. Enterica Serovar Typhimurium ; Transferases ; Proteins ; Escherichia Coli K12 ; Antigens ; Caulobacter Crescentus ; Mutants;
    ISSN: 1098-5530
    ISSN: 10985530
    ISSN: 00219193
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  • 2
    Language: English
    In: Journal of Bacteriology, May, 2012, Vol.194(9-10), p.2646(12)
    Description: Escherichia coli K-12 WcaJ and the Caulobacter crescentus HfsE, PssY, and PssZ enzymes are predicted to initiate the synthesis of colanic acid (CA) capsule and holdfast polysaccharide, respectively. These proteins belong to a prokaryotic family of membrane enzymes that catalyze the formation of a phosphoanhydride bond joining a hexose-1-phosphate with undecaprenyl phosphate (Und-P). In this study, in vivo complementation assays of an E. coli K-12 wcaJ mutant demonstrated that WcaJ and PssY can complement CA synthesis. Furthermore, WcaJ can restore holdfast production in C. crescentus. In vitro transferase assays demonstrated that both WcaJ and PssY utilize UDP-glucose but not UDP-galactose. However, in a strain of Salmonella enterica serovar Typhimurium deficient in the WbaP O antigen initiating galactosyltransferase, complementation with WcaJ or PssY resulted in O-antigen production. Gas chromatography-mass spectrometry (GC-MS) analysis of the lipopolysaccharide (LPS) revealed the attachment of both CA and O-antigen molecules to lipid A-core oligosaccharide (OS). Therefore, while UDP-glucose is the preferred substrate of WcaJ and PssY, these enzymes can also utilize UDP-galactose. This unexpected feature of WcaJ and PssY may help to map specific residues responsible for the nucleotide diphosphate specificity of these or similar enzymes. Also, the reconstitution of O-antigen synthesis in Salmonella, CA capsule synthesis in E. coli, and holdfast synthesis provide biological assays of high sensitivity to examine the sugar-1-phosphate transferase specificity of heterologous proteins.
    Keywords: Escherichia Coli -- Genetic Aspects ; Caulobacter -- Genetic Aspects ; Microbiological Synthesis -- Research ; Gas Chromatography -- Usage ; Glycosyltransferases -- Research
    ISSN: 0021-9193
    Source: Cengage Learning, Inc.
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  • 3
    In: BioMed Research International, 2013, Vol.2013, 8 pages
    Description: . To evaluate the role of key enzymes in the methionine-homocysteine metabolism (MHM) in the physiopathology of preeclampsia (PE). . Plasma and placenta from pregnant women (32 controls and 16 PE patients) were analyzed after informed consent. Protein was quantified by western blot. RNA was obtained with RNA purification kit and was quantified by reverse transcritase followed by real-time PCR (RT-qPCR). Identification of the C677T and A1298C methylenetetrahydrofolate reductase (MTHFR) single-nucleotide polymorphisms (SNPs) and A2756G methionine synthase (MTR) SNP was performed using PCR followed by a high-resolution melting (HRM) analysis. S-adenosyl methionine (SAM) and S-adenosyl homocysteine (SAH) were measured in plasma using high-performance liquid chromatography-tandem mass spectrometry (HPLC/MS/MS). The SNP association analysis was carried out using Fisher’s exact test. Statistical analysis was performed using a Mann-Whitney test. . RNA expression of MTHFR and MTR was significantly higher in patients with PE as compared with controls. Protein, SAM, and SAH levels showed no significant difference between preeclamptic patients and controls. No statistical differences between controls and PE patients were observed with the different SNPs studied. . The RNA expression of MTHFR and MTR is elevated in placentas of PE patients, highlighting a potential compensation mechanism of the methionine-homocysteine metabolism in the physiopathology of this disease.
    Keywords: Research Article
    ISSN: 2314-6133
    E-ISSN: 2314-6141
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  • 4
    Language: Portuguese
    In: Revista médica de Chile, 01 October 2014, Vol.142(10), pp.1284-1290
    Description: Background: Early HIV (human immunodeficiency virus) diagnosis optimizes therapies aimed at reducing viral load, increasing survival, lowering health costs and reducing the number of people infected with the virus. In Chile, despite widespread and readily available HIV testing, infected people continue to get tested in a late fashion and are usually diagnosed in advanced stages of the disease. Aim: To determine the elements that facilitate or impede a timely HIV testing and to evaluate how to improve the access to HIV testing. Material and Methods: Descriptive, in-depth interviews to 30 participants with unknown serology, 15 participants diagnosed at AIDS stage and 15 health care professionals working at a primary healthcare settings. Results: Users and professionals formulated three suggestions to improve timely access to ELISA test for HIV diagnosis. Namely, to inform users and professionals about the characteristics of the disease and diagnostic test, to offer fast and easy access to HIV testing, and to train the whole healthcare team about obtaining informed consent for testing. Conclusions: These recommendations should be implemented at healthcare centers to attain a timely HIV diagnosis.
    Keywords: Medicine, General & Internal ; Early Diagnosis ; HIV ; Qualitative Research ; Medicine
    ISSN: 0034-9887
    E-ISSN: 0717-6163
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  • 5
    Language: English
    In: The Astrophysical Journal Letters, 2016, Vol.824(1), p.L1 (7pp)
    Description: Our current understanding of galaxy evolution still has many uncertainties associated with the details of the accretion, processing, and removal of gas across cosmic time. The next generation of radio telescopes will image the neutral hydrogen (H i ) in galaxies over large volumes at high redshifts, which will provide key insights into these processes. We are conducting the COSMOS H i Large Extragalactic Survey (CHILES) with the Karl G. Jansky Very Large Array, which is the first survey to simultaneously observe H i from z = 0 to z ∼ 0.5. Here, we report the highest redshift H i 21 cm detection in emission to date of the luminous infrared galaxy COSMOS J100054.83+023126.2 at z = 0.376 with the first 178 hr of CHILES data. The total H i mass is (2.9 ± 1.0) × 10 10 M ⊙ and the spatial distribution is asymmetric and extends beyond the galaxy. While optically the galaxy looks undisturbed, the H i distribution suggests an interaction with a candidate companion. In addition, we present follow-up Large Millimeter Telescope CO observations that show it is rich in molecular hydrogen, with a range of possible masses of (1.8–9.9) × 10 10 M ⊙ . This is the first study of the H i and CO in emission for a single galaxy beyond z ∼ 0.2.
    Keywords: Astrophysics - Astrophysics Of Galaxies;
    ISSN: 2041-8205
    E-ISSN: 2041-8213
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  • 6
    In: Monthly Notices of the Royal Astronomical Society, 2018, Vol. 484(2), pp.2234-2256
    Description: We present a study of 16 H   i -detected galaxies found in 178 h of observations from Epoch 1 of the COSMOS H  i Large Extragalactic Survey (CHILES). We focus on two redshift ranges between 0.108 ≤ $z$ ≤ 0.127 and 0.162 ≤ $z$ ≤ 0.183 which are among the worst affected by radio frequency interference (RFI). While this represents only 10 per cent of the total frequency coverage and 18 per  cent of the total expected time on source compared to what will be the full CHILES survey, we demonstrate that our data reduction pipeline recovers high-quality data even in regions severely impacted by RFI. We report on our in-depth testing of an automated spectral line source finder to produce H  i total intensity maps which we present side-by-side with significance maps to evaluate the reliability of the morphology recovered by the source finder. We recommend that this become a common place manner of presenting data from upcoming H  i surveys of resolved objects. We use the COSMOS 20k group catalogue and extract the filamentary structure using the topological DisPerSE algorithm to evaluate the H  i morphology in the context of both local and large-scale environments and we discuss the shortcomings of both methods. Many of the detections show disturbed H  i morphologies suggesting they have undergone a recent interaction which is not evident from deep optical imaging alone. Overall, the sample showcases the broad range of ways in which galaxies interact with their environment. This is a first look at the population of galaxies and their local and large-scale environments observed in H  i by CHILES at redshifts beyond the $z$  = 0.1 Universe.
    Keywords: Galaxies: Evolution ; Galaxies: Groups: General ; Galaxies: Ism ; Radio Lines: Galaxies
    ISSN: 0035-8711
    E-ISSN: 1365-2966
    Source: Oxford University Press
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  • 7
    In: Revista Chilena de Salud Pública, 05/09/2011, Vol.14(2/3)
    Description: Alimentación saludable en universitarios, casino Los Notros, Universidad de la Frontera, 2009 Centro de salud docente-asistencial rural modelo Universidad de la Frontera, Chile Diagnóstico de salud escolar utilizando ficha Clap-OPS Experiencia de interacción fronteriza en acciones de promoción y prevención en alcohol y drogas Fomento y promoción en salud escolar: Un desafío pendiente Fortalecimiento de maternidades cercanas a la familia: pabellón docente en el Servicio de Salud Ñuble Integralidad del modelo biopsicosocial en la formación de estudiantes de enfermería, Universidad Católica del Norte Jóvenes y niños insulino dependientes aprenden técnicas de autocuidado en campamento Odontología y nutrición: dos disciplinas complementarias y sinérgicas Proyectos de extensión universitaria desarrollados a través de prácticas profesionales de disciplinas de la salud
    ISSN: 0717-3652
    ISSN: 07195281
    E-ISSN: 0717-3652
    E-ISSN: 07195281
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  • 8
    Language: Spanish
    In: Archivos de Medicina Veterinaria, 1995, Vol.(1), pp.53-59
    Description: Se establecio la presencia de los factores enterotoxico y "cytolethal distending toxin" (CDLT) en 13 cepas de C. jejuni (8 correspondieron al biotipo I y 5 al II) y 8 cepas de C. coli (1 correspondio al biotipo I y 7 al II), aisladas de bovinos con y sin trastornos entericos. utilizando la tecnica del asa intestinal de rata y cultivos de celulas HeLa, respectivamente. El filtrado de cultivos del 77% de las cepas de C. jejuni y del 75% de C. coli presentaron capacidad enterotoxica. A su vez, el 69.2% de las suspensiones celulares de las cepas de C. jejuni y el 75% de C. coli presentaron capacidad acumuladora de fluido. En relacion al factor CLDT se pudo establecer que el 81% de las cepas ensayadas, todas las C. jejuni y el 50% de las de C. coli, presentaron este factor. De acuerdo a las propiedades patogenicas expresadas por las cepas en estudio, el patovar F-S+C+ fue el mas frecuente, correspondiendo a 8 cepas de C. jejuni y 1 de C. coli con capacidad acumuladora de fluido mediante sus suspensiones celulares y que, ademas, presentaron el factor CDLT ; Sumarios (En, Es) ; 30 ref.
    Keywords: Cattle ; Campylobacter Jejuni ; Enterotoxins ; Campylobacter ; Bovin ; Campylobacter Jejuni ; Enterotoxine ; Campylobacter
    ISSN: 0301-732X
    Source: AGRIS (Food and Agriculture Organization of the United Nations)
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  • 9
    Language: English
    In: Journal of Infection, December 2015, Vol.71(6), pp.627-641
    Description: To evaluate the course of left-sided infective endocarditis (LsIE) in patients with liver cirrhosis (LC) analyzing its influence on mortality and the impact of surgery. Prospective cohort study, conducted from 1984 to 2013 in 26 Spanish hospitals. A total of 3.136 patients with LsIE were enrolled and 308 had LC: 151 Child–Pugh A, 103 B, 34 C and 20 were excluded because of unknown stage. Mortality was significantly higher in the patients with LsIE and LC (42.5% vs. 28.4%; p 〈 0.01) and this condition was in general an independent worse factor for outcome (HR 1.51, 95% CI: 1.23–1.85; p 〈 0.001). However, patients in stage A had similar mortality to patients without cirrhosis (31.8% vs. 28.4% p = NS) and in this stage heart surgery had a protective effect (28% in operated patients vs. 60% in non-operated when it was indicated). Mortality was significantly higher in stages B (52.4%) and C (52.9%) and the prognosis was better for patients in stage B who underwent surgery immediately (mortality 50%) compared to those where surgery was delayed (58%) or not performed (74%). Only one patient in stage C underwent surgery. Patients with liver cirrhosis and infective endocarditis have a poorer prognosis only in stages B and C. Early surgery must be performed in stages A and although in selected patients in stage B when indicated.
    Keywords: Liver Cirrhosis ; Left-Sided Infectious Endocarditis ; Risk Stratification ; Prognostic ; Surgery ; Medicine ; Public Health
    ISSN: 0163-4453
    E-ISSN: 1532-2742
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  • 10
    Language: Spanish
    In: Homenaje a Pedro Montserrat, 1988-01-01, ISBN 84-86856-13-2, pags. 143-148, 1988, pp.143-148
    Source: Fundación Dialnet
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