Format:
1 Online-Ressource (212 pages)
ISBN:
9783319527154
Series Statement:
Current Topics in Microbiology and Immunology v.402
Content:
Preface -- Photorhabdus as a Bug for All Seasons -- Bugs that Kill Bugs -- Bugs that do Drugs -- Bugs that Infect Both Insects and Man -- Bugs that Just Keep Giving -- Contents -- Insecticidal Toxins and Virulence Regulation -- 55 Insecticidal Toxin Complexes from Photorhabdus luminescens -- Abstract -- 1 Introduction -- 1.1 The Life Cycle of Insecticidal Nematodes -- 1.2 Specificity of Insecticidal Toxins -- 2 'Toxin Complex' (Tc) Toxins -- 2.1 Nomenclature of Tc Toxins -- 2.2 TcA-like Proteins of the Toxin Complex -- 2.2.1 Interaction of TcA-like Proteins with the Host Cell Membrane -- 2.2.2 The Molecular and Atomic Structure of TcA-like Proteins -- 2.2.3 Is TcA per se Insecticidal? -- 2.3 TcB-like Proteins of the Toxin Complex -- 2.4 TcC-like Proteins of the Toxin Complex -- 2.5 Structure of the Complete TC Complex -- 3 Biological Activities of Tc Toxins -- 3.1 TccC3 Is an ADP-Ribosyltransferase that Targets Actin -- 3.1.1 Modification of Actin by TccC3 at Threonine148 -- 3.1.2 Photorhabdus Toxin Photox Modifies Actin at Arginine177 -- 3.2 TccC5 Is an ADP-Ribosyltransferase that Targets Rho Proteins -- 3.2.1 Rho Proteins Are Substrates of TccC5 -- 3.2.2 Functional Consequences of Rho ADP-Ribosylation by TccC5 -- 4 Conclusions -- References -- 51 Identifying Anti-host Effectors in Photorhabdus -- Abstract -- 1 Introduction -- 2 A Forward Genetics Approach -- 2.1 Rapid Virulence Annotation -- 2.2 Secondary Metabolites as Virulence Factors -- 2.3 Host Specificity -- 3 The Drosophila Embryo System -- 4 Immune Cell Phenotype Screen -- 5 In Silico Screens -- 5.1 Photox-A Toxin Unique to P. luminescens -- 5.2 PaTox-A Toxin Unique to P. asymbiotica -- 5.3 The Photorhabdus Virulence Cassettes -- 6 Future Approaches -- References
Content:
53 Flagellar Regulation and Virulence in the Entomopathogenic Bacteria-Xenorhabdus nematophila and Photorhabdus luminescens -- Abstract -- 1 Bacterial Motility and Virulence in Pathogenic Bacteria -- 2 Flagella Affect Virulence by Facilitating Motility -- 3 Flagellar Regulators Affect Virulence by Regulating Non-flagellar Virulence Factors -- 4 The Flagella Apparatus as Virulence Factor Secretion System -- 5 In Situ Flagellar Gene Expression During Invertebrate Infection -- 6 Phenotypic Variation and Bimodal Expression of Flagellar and Virulence Genes -- 7 Conclusions and Future Work on P. luminescens -- References -- Secondary Metabolism and its Regulation -- 24 Natural Products from Photorhabdus and Other Entomopathogenic Bacteria -- Abstract -- 1 Introduction -- 2 Non-ribosomal Peptide Synthetases (NRPS) and Related Biosynthesis Pathways -- 3 Overview on Biosynthesis Gene Clusters and Natural Products in Different Photorhabdus Strains -- 4 Tools for the Identification of Natural Products -- 4.1 Promoter Exchange -- 4.2 Manipulation of Regulatory Proteins -- 4.3 Heterologous Expression -- 4.4 Chemical Tools -- 5 Conclusions -- References -- 21 The Regulation of Secondary Metabolism in Photorhabdus -- Abstract -- 1 Introduction -- 2 The Photorhabdus Life Cycle -- 3 Secondary Metabolism, Pathogenicity and Mutualism -- 4 Phenotypic Variation, Phase Variation and Secondary Metabolism -- 5 The Regulation of Secondary Metabolism -- 5.1 Global Regulators -- 5.1.1 HexA -- 5.1.2 The BarA-UvrY Two-Component Pathway -- 5.1.3 Magic Spot-(P)ppGpp and Nutrient Limitation -- 5.1.4 Metabolism and the Metabolic Switch -- 5.1.5 Quorum Sensing and AI-2 -- 5.2 Pathway-specific Regulators -- 5.2.1 AQ -- 5.2.2 Stilbene -- 5.2.3 Bioluminescence -- 6 Conclusions -- References -- 28 Quorum Sensing and LuxR Solos in Photorhabdus -- Abstract
Content:
1 Quorum Sensing in Gram-Negative Bacteria -- 2 LuxR Solos -- 2.1 AHL-LuxR Solos -- 2.2 PluR/PauR-LuxR Solos -- 2.3 PAS4-LuxR Solos -- 3 Quorum Sensing in Photorhabdus -- 3.1 The PpyS/PluR Quorum-Sensing System of P. luminescens and P. temperata -- 3.2 The DarABC/PauR Quorum Sensing System of P. asymbiotica -- 4 Outlook and Future Perspectives -- Acknowledgments -- References -- Insect Immunity -- 52 Insect Immunity to Entomopathogenic Nematodes and Their Mutualistic Bacteria -- Abstract -- 1 Introduction -- 2 Entomopathogenic Nematode-Bacteria Complexes -- 3 Insect Innate Immune Responses -- 4 Insect Immune Response to Mutualistic Bacteria -- 5 Immune Evasion by Mutualistic Bacteria -- 6 Immune Defense Against Entomopathogenic Nematodes -- 7 Immune Evasion by Entomopathogenic Nematodes -- 8 Perspectives and Future Directions -- Acknowledgments -- References -- Photorhabdus Asymbiotica and Human Pathogenicity -- 29 Photorhabdus asymbiotica as an Insect and Human Pathogen -- Abstract -- 1 Introduction -- 2 Insights from the P. asymbiotica Genome -- 3 P. asymbiotica as an Insect Pathogen -- 4 The Human Host -- 4.1 Overcoming Host Defences -- 4.2 Metabolic Adaptations -- 5 Conclusions -- References -- 56 A Review of Clinical Cases of Infection with Photorhabdus Asymbiotica -- Abstract -- 1 Background -- 2 Epidemiology -- 2.1 Age and Gender -- 2.2 Pre-morbid Status -- 2.3 Geographical Distribution -- 2.4 Environmental Exposure -- 2.5 Incidence of Human Infection -- 3 Recognition of P. asymbiotica in the Clinical Diagnostic Laboratory -- 4 Clinical Features -- 5 Treatment -- 6 Conclusions -- References -- Practical Guides -- 57 A Practical Guide to Recombineering in Photorhabdus and Xenorhabdus -- Abstract -- 1 Introduction to 'Recombineering' -- 2 Reagents and Equipment Needed for Practical 'Recombineering' -- 2.1 Reagents
Content:
2.2 Equipment Needed for 'Recombineering' -- 3 Pluγβα Recombineering in Photorhabdus and Xenorhabdus -- 3.1 Materials Needed -- 3.2 Method -- 3.2.1 General Schema for the Pipeline -- 3.2.2 Preparation of 'Knock-In' or 'Knockout' Cassette -- 3.2.3 PCR Purification and Yield -- 3.2.4 Preparation of P. luminescens and X. stockiae Electrocompetent Cells -- 3.2.5 Transformation of the Recombinase Expression Plasmid -- 3.2.6 Identify the Transformants -- 3.2.7 Genome Engineering of the P. luminescens and X. stockiae -- 3.2.8 Seamless Mutagenesis of the P. luminescens and X. stockiae -- 4 Rapid Construction of Knock-in Vectors Using the recET System -- 4.1 Materials -- 4.2 Method -- 4.2.1 General Scheme of the Pipeline -- 4.2.2 Fragment Assembly -- 4.2.3 Multiple Fragments Assembly Using the recET System -- 5 Concluding Remarks -- References
Additional Edition:
9783319527147
Additional Edition:
Print version ffrench-Constant, Richard H The Molecular Biology of Photorhabdus Bacteria Cham : Springer International Publishing,c2017 9783319527147
Language:
English
URL:
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