Kooperativer Bibliotheksverbund

UID:

Format: 1 online resource (297 p.)

Edition: 1st ed.

ISBN: 1-282-25742-0 , 9786613814999 , 0-12-385873-9

Content: The imaging of small cellular components requires powerful instruments, and an entire family of equipment and techniques based on the confocal principle has been developed over the past 30 years. Such methods are commonly used by neuroscience researchers, but the majority of these users do not have a microscopy or a cell biology backgrounds and do can encounter difficulties in obtaining and interpreting results. This volume brings experts in high-resolution optical microscopy applications in neuroscience and cell biology together to document the state of the art. Outlining what is currently

Note: Description based upon print version of record. , Front Cover; Cellular Imaging Techniques for Neuroscience and Beyond; Copyright Page; Contents; List of Contributors; 1 Confocal Laser Scanning: of Instrument, Computer Processing, and Men; Introduction; Pinhole, Depth of Focus, and Laser Illumination; When/Why Does One Need a CLSM?; Abbe, Shannon, and Nyquist; Imaging of a 2D Line and Deblurring; Axial Resolution; Resolution and Sampling; Signal Separation, Orders of Magnitude, and Resolution Limits; Confocal Microscopy Further Considered; Cross Talk Awareness; Excitation Cross Talk; Elimination of Cross Talk , Biological Objects Translated to PixelsHigh-probability Determination of Diameter; Best-Fit Object 3D Recognition; Automated Objective Threshold Analysis; Why Does a 3D Reconstructed Cell Resemble a Pancake?; Touch; Actual Experiment; Computer Software to Define a Contact; Synaptic Contacts: Extra Marker; Colocalization; Conclusion; Acknowledgments; References; 2 Beyond Abbe's Resolution Barrier: STED Microscopy; Introduction; A New Wave of Imaging; STED Microscopy: The Basic Concept; Implementation of STED Microscopy; The Microscope Base; Laser Sources; The Doughnut; Synchronization , Objective LensesEmission Detection; Scanning Schemes; Software; Stability Considerations; Checking the Resolution; Sine Qua Non: Speed, Color, Depth, Live Imaging; Temporal Resolution and Imaging Speed; Labeling Strategies; Multicolor Imaging; Depth Penetration and Spatial Resolution; Spatial Resolution in z; Live-cell Imaging; Summary and Outlook; Reversible Saturable Optical Fluorescence Transitions: A More General Principle for Nanoscopy; Other Areas of Development; References; 3 Enhancement of Optical Resolution by 4pi Single and Multiphoton Confocal Fluorescence Microscopy; Introduction , The 4pi Principle and SetupMicroscope Alignment; Initial Alignment of the Excitation by Eye; Optimizing the Coverslip Correction Ring; Alignment of the Bottom Lens (XYZ); Alignment of the Second Mirror (XYZ); 4pi Imaging; 4pi Deconvolution; Sample Preparation; Fixation; Selection of Fluorescent Dyes; Microtubule and Microtubule Plus End Imaging; Visualization of DNA; 4pi Imaging of Muntjac Chromosomes; Single-photon Excitation (Measurement of the Redox State in Dopamine Neurons); SYCP3 Axis as a Marker for Chromatin Organization in Mouse Spermatocytes; Microbubbles with Medicine , Future of 4pi ImagingAcknowledgment; References; 4 Nano Resolution Optical Imaging Through Localization Microscopy; Introduction; Superresolution Microscopy Techniques; The Concept Behind Localization Microscopy; Matters of Concern; Localization Precision; Linkage Error; Specificity; Labeled Fraction; Labeling Density; The Main Approaches to Single-molecule Localization-based Superresolution Microscopy; Using Fluorescent Proteins: PALM and FPALM; Using a Pair of Interacting Cyanine Dyes: STORM; Using Blinking Dyes: dSTORM; Using High-intensity Light: GSDIM; Using Targeted Molecules: BALM , Fluorescent Probes , English

Additional Edition: ISBN 0-12-385872-0

Language: English

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Format: XIV, 698 p. 190 illus., 71 illus. in color. , online resource.

Edition: 3rd ed. 2006.

ISBN: 9780387289427

Content: "The content of the book also serves to emphasize that neuroanatomy is, perhaps more than ever, a thriving and important part of the neurosciences...Most contributions have in common the combination of modern tract-tracing methods with other means of characterizing neural tissue, and thus the book also serves to highlight the gradual disappearance of borders between traditional neuroanatomy and other approaches to the study of the nervous system...this book should continue to be a useful source of information, and deserves to be available in all laboratories applying or considering to apply neuroanatomical methods." P. Brodal, Neuroscience Vol.40, No. 1 .

Note: Short Retrospection -- Preembedding Immunoelectron Microscopy: Applications for Studies of the Nervous System -- Postembedding Immunogold Cytochemistry of Membrane Molecules and Amino Acid Transmitters in the Central Nervous System -- Cell and Tissue Microdissection in Combination with Genomic and Proteomic Applications -- Molecules and Membrane Activity: Single-Cell RT-PCR and Patch-Clamp Recording from Central Neurons -- Merging Structure and Function: Combination of In Vivo Extracellular and Intracellular Electrophysiological Recordings with Neuroanatomical Techniques -- Juxtacellular Labeling of Individual Neurons In Vivo: From Electrophysiology to Synaptology -- Nonradioactive In Situ Hybridization in Combination with Tract-Tracing -- Viral Tracers for the Analysis of Neural Circuits -- Dextran Amines: Versatile Tools for Anterograde and Retrograde Studies of Nervous System Connectivity -- Multiple Neuroanatomical Tract-Tracing: Approaches for Multiple Tract-Tracing -- Tract-Tracing in Developing Systems and in Postmortem Human Material Using Carbocyanine Dyes -- Combined Fluorescence Methods to Determine Synapses in the Light Microscope: Multilabel Confocal Laser Scanning Microscopy -- Advances in Understanding Cortical Function Through Combined Voltage-Sensitive Dye Imaging, Whole-Cell Recordings, and Analysis of Cellular Morphology -- From Dendrites to Networks: Optically Probing the Living Brain Slice and Using Principal Component Analysis to Characterize Neuronal Morphology -- Stereology of Neural Connections: An Overview -- Three-Dimensional Computerized Reconstruction from Serial Sections: Cell Populations, Regions, and Whole Brain -- Atlases of the Human Brain: Tools for Functional Neuroimaging -- Neuron and Network Modeling -- Functional Connectivity of the Brain: Reconstruction from Static and Dynamic Data.

In: Springer Nature eBook

Additional Edition: Printed edition: ISBN 9781441939630

Additional Edition: Printed edition: ISBN 9780387509655

Additional Edition: Printed edition: ISBN 9780387289410

Language: English

DOI: 10.1007/0-387-28942-9

URL: https://doi.org/10.1007/0-387-28942-9

UID:

Format: II, 6 S , Ill., graph. Darst., Kt

Series Statement: Lithostratigraphic series 6

Language: Undetermined

UID:

Format: Losebl.-Ausg.

ISBN: 0-444-89539-6

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Format: 78 S. : , zahlr. Ill.

ISBN: 3-437-11348-8 , 1-56081-307-5

Series Statement: Progress in histochemistry and cytochemistry 22,1

Note: Literaturverz. S. 64 - 75

Language: English

Subjects: Biology

Keywords: Gartenbohne ; Leukoagglutinin ; Nervennetz ; Neuronenverschaltung ; Gartenbohne ; Leukoagglutinin ; Indikatormethode ; Nervennetz ; Anatomie ; Lectine

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Format: 1 online resource (xviii, 347 p., [5] p. of plates) : , ill. (some col.)

ISBN: 9780191724626 (ebook) : , 0191724629 (ebook) :

Content: This text examines the architecture and activity of parahippocampal area of the temporal lobe, describes the systems active in memory, perception and behaviour, and outlines the significance of its involvement in the progress of a range of disease states.

Additional Edition: Print version ISBN 9780198509172

Language: English

URL: Oxford scholarship online

UID:

Format: XVIII, 347 S , Ill., graph. Darst , 24 cm

ISBN: 0198509170

Note: Includes bibliographical references and index

Language: English

Subjects: Biology

Keywords: Entorhinalcortex ; Temporallappen ; Hippocampus

URL: Inhaltsverzeichnis

URL: Verlagsangaben

URL: http://www.loc.gov/catdir/enhancements/fy0615/2003276254-t.html