Engineering in Life Sciences, 2015, Vol.15(2), p.243(11)
To purchase or authenticate to the full-text of this article, please visit this link: http://onlinelibrary.wiley.com/doi/10.1002/elsc.201400208/abstract Byline: Raghda Alakhras, Stamatia Bellou, Grammatiki Fotaki, Georgia Stephanou, Nikos A. Demopoulos, Seraphim Papanikolaou, George Aggelis Keywords: Cytotoxicity; Gamma linolenic acid; Genotoxicity; HL-60 leukemic cells; Lipid accumulation Polyunsaturated fatty acids, especially gamma linolenic acid (GLA), are potentially useful agents in the treatment of cancer. Cunninghamella echinulata, a fungus species that is able to synthesize GLA, when cultivated under nitrogen-limited conditions in a medium having glucose as carbon and energy source, accumulated 32-35% of lipids containing 11-18% GLA. The conversion yield of glucose to lipid was around 0.11 g per gram of glucose consumed while the lipid production was 5 g/L. Fatty acid lithium salts (FALS) were prepared from the total Cunninghamella lipids and studied for their effects on HL-60 human leukemic cells. Cytotoxicity of FALS on HL-60 leukemic cells was linearly related to the FALS concentration. High FALS concentration (i.e. 15 and 20 I1/4g/mL) induced DNA fragmentation, while concurrent treatment of cells with H.sub.2O.sub.2 (at 100 I1/4M) and FALS resulted in enhanced cytotoxicity of H.sub.2O.sub.2. However, when FALS were employed at low concentrations (i.e. 5 and 10 I1/4g/mL), they demonstrated a protective effect on HL-60 cells against H.sub.2O.sub.2 genotoxicity, whereas at 20 I1/4g/mL FALS enhanced the ability of H.sub.2O.sub.2 to induce DNA fragmentation. It is concluded that FALS derived from C. echinulata lipids could be an effective preparation against HL-60 human leukemic cells. Supporting information: Additional Supporting Information may be found in the online version of this article As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. CAPTION(S): Figure S1 Figure S2
Glucose ; Linolenic Acids ; Leukemia
Cengage Learning, Inc.