Kooperativer Bibliotheksverbund

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Format: 1 videorecording (57 min., 23 sec.) , 005723

Note: Retrieved April 11, 2024, from https://hstalks.com/bs/406/. , Introduction -- Calcium is an ubiquitous messenger -- Spatial control of calcium -- Calcium-reporting dyes -- Basic mechanisms of calcium regulation -- Why have internal calcium stores and release? -- Some organelles that serve as calcium stores -- Ca mobilization by hormones/ neurotransmitters -- Calcium mobilizing messengers -- Intracellular signaling by IP3 -- Structures of calcium mobilizing messengers -- Multiple Ca release mechanisms -- cADPR activity was discovered in sea urchin eggs -- Studying Ca release in cell-free systems -- Ca release in urchin egg homogenates -- Role of cADPR in calcium signaling -- Second messenger roles for cADPR -- cADPR/NAADP synthesis, analogues and drugs -- Synthesis of cADPR and analogues -- Caged cADPR photolysis induces Ca waves -- Caged Ca photolysis evokes local Ca rise -- Potentiation of CICR by cADPR -- Ryanodine receptors: function -- Ryanodine receptors: imaging elementary events -- Photolysis released cADPR and Ca transients -- cADPR antagonist abolishes Ca waves -- cADPR increases the frequency of Ca sparks -- Mechanism of enhanced Ca release by cADPR -- Isoprenaline-evoked increases in cADPR levels -- Pharmacology of cADPR increases -- Role of cADPR in cardiac E-C coupling -- Summary of cADPR activity -- cADPR in Ca signaling -- What is NAADP -- Photolysing caged NAADP results in a Ca wave -- Spatial sensitivity to low [NAADP] -- Effect of CICR channels inhibition -- NAADP: spatial/ temporal aspects of Ca signals -- NAADP stores are separate from the ER -- Stratification of sea urchin eggs -- GPN rupture lysosomes -- Lysotracker -- GPN ruptures lysosomes and releases Ca -- NAADP is an initiator: Ca-induced Ca release -- NAADP releases Ca from lysosomal stores -- Organelle marker enzymes -- Receptor distribution -- Ca uptake into NAADP-sensitive stores -- NAADP: special channel or special regulation? -- Inhibition of NAADP-induced Ca release -- NAADP-induced calcium release -- NAADP-induced Ca release amplification by RyR -- Bafilomycin inhibits NAADP-induced Ca release -- Interactions between NAADP and CICR channels -- Auto-inactivation of NAADP-induced Ca release -- Specificity of receptor-mediated NAADP signaling -- NAADP triggers global Ca signals -- CCK-evoked calcium spiking blocked by GPN -- CCK-evoked calcium spiking blocked: bafilomycin -- Selectivity in GPN blockage -- Radio-receptor assay for NAADP levels -- CCK-induced changes in NAADP / cADPR levels -- NAADP: trigger hypothesis -- Receptor specificity in pancreatic acinar cells -- ARC can synthesize cADPR and NAADP -- NAADP changes in CD38-/- mouse -- NAADPR is easily solubilized as a native protein -- Affinity purification of the NAADP receptor -- NAADP binding proteins -- Summary.

Language: English