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  • 1
    Language: English
    In: Journal of the American Chemical Society, 12 September 2012, Vol.134(36), pp.14678-81
    Description: A simple and ultrasensitive colorimetric DNA assay based on the detection of the product of a ligation chain reaction (LCR) and the use of gold nanoparticles (AuNPs) as signal generators has been developed. During LCR, the AuNPs were ligated together, resulting in a distinct color change in real time after a sufficient number of thermal cycles. The cumulative nature of the protocol produced a detection limit of 20 aM with a selectivity factor of 10(3).
    Keywords: DNA -- Analysis ; Gold -- Chemistry ; Metal Nanoparticles -- Chemistry
    ISSN: 00027863
    E-ISSN: 1520-5126
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  • 2
    Language: English
    In: Applied Surface Science, 15 May 2012, Vol.258(15), pp.5574-5578
    Description: ► Polyamide 6 films were treated with different environmental humidity, respectively. ► We investigated the effect of different environmental humidity on the surface modification of atmospheric pressure plasma. ► The environmental humidity has effectiveness on the polymer surface modification with atmospheric pressure plasma. The environmental humidity (EH) may have potential influence on atmospheric pressure plasma treatment. In order to investigate how the environmental humidity affects atmospheric pressure plasma treatment, polyamide 6 (PA 6) films were treated by helium/oxygen (He/O ) plasmas using atmospheric pressure plasma jet (APPJ) at different environmental humidity. The plasma treated samples had lower contact angles than the control. Atomic force microscopy (AFM) showed increased surface roughness, while X-ray photoelectron spectroscopy (XPS) revealed increased oxygen contents after the plasma treatments. The plasma treated films had higher T-peel strength than that of the control as revealed by T-peel strength tests. It was shown that the addition of environmental humidity increased effectiveness of the plasma in polymer surface modification after the treatment.
    Keywords: Plasma ; Polyamide ; Environmental Humidity (Eh) ; Xps ; Engineering
    ISSN: 0169-4332
    E-ISSN: 1873-5584
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  • 3
    Language: English
    In: Analytical chemistry, 01 February 2011, Vol.83(3), pp.820-7
    Description: A highly sensitive microRNA (miRNA) biosensor that employs ruthenium oxide nanoparticle (RuO(2) NP)-initiated polymerization of 3,3'-dimethoxybenzidine (DB) and miRNA-templated deposition of an insulating poly(3,3'-dimethoxybenzidine) (PDB) film is described in this work. The biosensor was made of a mixed monolayer of oligonucleotide capture probes (CPs) and 4-mercaptoaniline on a gold electrode. Following hybridization with a RuO(2) NP-tagged target miRNA, a mixture of DB/H(2)O(2) in pH 5.0 0.10 M acetate buffer was applied to the biosensor. The RuO(2) NPs serve as polymerization initiator/catalyst for the polymerization of DB. And the hybridized anionic miRNA strands and free CPs serve as templates, guiding the deposition of PDB. The amount of the deposited PDB and its insulating power directly correlated to the concentration of the target miRNA in solution. Electrochemical impedance spectroscopic tests showed that a linear charge-transfer resistance-concentration relationship from 6.0 fM to 2.0 pM was attained after 60 min of incubation in the DB/H(2)O(2) mixture. There was no cross-hybridization between pre-miRNA and mature miRNA and very little cross-hybridization among closely related miRNA family members even at single-base-mismatched levels. This impedance-based biosensor offers an attractive alternative for miRNA expression profiling and may enable the development of a portable multiplexing miRNA profiling system.
    Keywords: Biosensing Techniques -- Methods ; Metal Nanoparticles -- Chemistry ; Micrornas -- Analysis ; Nucleic Acid Amplification Techniques -- Methods ; Nucleic Acid Hybridization -- Methods ; Ruthenium Compounds -- Chemistry
    ISSN: 00032700
    E-ISSN: 1520-6882
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  • 4
    Language: English
    In: Applied Surface Science, 2011, Vol.257(14), pp.6068-6072
    Description: ► We treated polyamide 6 films using pure helium (He), He/O and He/CF , respectively. ► We investigated the effect of the different plasma gases treatment on the surface modification of atmospheric pressure plasma. ► The addition of O or CF to He plasma had different effectiveness on the polymer surface modification. To investigate the effect of the different plasma gases treatment on the surface modification of atmospheric pressure plasma, polyamide 6 films were treated using pure helium (He), He/O and He/CF , respectively. Atomic force microscopy (AFM) showed rougher surface, while X-ray photoelectron spectroscopy (XPS) revealed increased oxygen and fluorine contents after the plasma treatments. The plasma treated samples had lower water contact angles and higher T-peel strength than that of the control. The addition of small amount of O or CF to He plasma increases the effectiveness of the plasma treatment in polymer surface modification in terms of surface roughness, surface hydrophilic groups, etching rate, water contact angle and bonding strength.
    Keywords: Atmospheric Pressure Plasma ; Polyamide 6 ; Etching Rate ; Afm ; Xps ; T-Peel Strength ; Engineering
    ISSN: 0169-4332
    E-ISSN: 1873-5584
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  • 5
    Language: English
    In: Applied Surface Science, 15 January 2011, Vol.257(7), pp.2531-2535
    Description: In this study, polyamide 6 films were treated with different jet-to-substrate distances to investigate how it influenced the etching effect of plasma treatment. When the samples were too close or too far from the nozzle, the etching rate was almost not measurable. When the distance was 2 mm, the etching rate was larger than that of other distance. Decrease in contact angle was observed under 2 mm or 3 mm of jet-to-substrate distance. However, the contact angle had no change when jet-to-substrate distance was 1 mm or 6 mm. It can be seen that the peel strength increased when jet-to-substrate distance was 2 mm or 3 mm, and the peel strength was the largest when jet-to-substrate distance was 2 mm. However, the peel strength had no change when jet-to-substrate distance was 1 mm or 6 mm. These results were corresponding to SEM and XPS results.
    Keywords: Polyamide 6 Film ; Atmospheric Pressure Plasma Jet (Appj) ; Etching Rate ; Influence ; Distance ; Engineering
    ISSN: 0169-4332
    E-ISSN: 1873-5584
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  • 6
    Language: English
    In: 2014, Vol.9(11), p.e113115
    Description: This study aimed to establish a 3-dimensional dynamic quantitative facial motion analysis system, and then determine its accuracy and test-retest reliability. The system could automatically reconstruct the motion of the observational points. Standardized T-shaped rod and L-shaped rods were used to evaluate the static and dynamic accuracy of the system. Nineteen healthy volunteers were recruited to test the reliability of the system. The average static distance error measurement was 0.19 mm, and the average angular error was 0.29°. The measuring results decreased with the increase of distance between the cameras and objects, 80 cm of which was considered to be optimal. It took only 58 seconds to perform the full facial measurement process. The average intra-class correlation coefficient for distance measurement and angular measurement was 0.973 and 0.794 respectively. The results demonstrated that we successfully established a practical 3-dimensional dynamic quantitative analysis system that is accurate and reliable enough to meet both clinical and research needs.
    Keywords: Research Article ; Biology And Life Sciences ; Computer And Information Sciences ; Engineering And Technology ; Medicine And Health Sciences
    E-ISSN: 1932-6203
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  • 7
    In: Chemical Communications, 2015, Vol.51(32), pp.6928-6931
    Description: On the basis of enzyme-catalysed reduction of silver ions and consequent deposition of ultrathin silver shells on gold nanorods, a highly efficient signal amplification method for immunoassay is developed. For a model analyte prostate-specific antigen, a 10 4 -fold improvement over conventional enzyme-linked immunosorbent assay is accomplished by leveraging on the cumulative nature of the enzymatic reaction and the sensitive response of plasnomic gold nanorods to the deposition the silver shells.
    Keywords: Amplification ; Antigens ; Translating ; Nanorods ; Strategy ; Gold ; Deposition ; Immunoassay ; Miscellaneous Sciences (So) ; Chemical and Electrochemical Properties (MD) ; Chemical and Electrochemical Properties (Ep) ; Chemical and Electrochemical Properties (Ed) ; Chemical and Electrochemical Properties (EC);
    ISSN: 1359-7345
    E-ISSN: 1364-548X
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  • 8
    Language: English
    In: Analytical chemistry, 07 May 2013, Vol.85(9), pp.4784-9
    Description: On the basis of hybridized target microRNA (miRNA) strand initiated cleavage of hybridized deoxyribonucleic acid (DNA) capture probes (CPs) by a duplex-specific nuclease (DSN), a highly sensitive and selective label-free miRNA biosensor is developed in this article. Briefly, thiolated DNA CPs are immobilized onto a gold electrode through self-assembly. The electrode is then hybridized to a sample solution containing the target miRNA. The hybridized CPs in the miRNA-CP duplexes are simultaneously cleaved by the DSN, releasing the target miRNA strands back to the sample solution. The released target miRNA strands again hybridize with the remaining CPs on the electrode, thus forming an isothermal amplification cycle. The distinct difference in electrochemical impedance between a control and the DSN cleaved biosensor allows label-free detection of miRNA down to femtomolar levels. The mismatch discrimination ability of the DSN permits miRNA expression to be profiled with high selectivity. The exceptional amplification power of the DSN along with the simple assay protocol makes direct miRNA expression profiling possible in real-world samples with minimal or no sample pretreatments. Attempts are made in direct profiling circulating miRNAs in serum and miRNAs in total RNA extracted from cancer cells.
    Keywords: Biosensing Techniques ; Electrochemical Techniques ; Micrornas -- Blood
    ISSN: 00032700
    E-ISSN: 1520-6882
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  • 9
    Language: English
    In: Analytical chemistry, 01 June 2011, Vol.83(11), pp.4090-4
    Description: Extensive secondary structures in nucleic acid targets seriously impede the binding of complementary oligonucleotide probes. We report here a method to conduct the detection under extremely low salt conditions where the secondary structures are less stable and more accessible. A new type of nanoparticle probes prepared by functionalizing gold nanoparticles with nonionic morpholino oligos is employed. Because of the salt-independent hybridization of the probes with nucleic acid targets, nanoparticle assemblies can be formed in 2 mM Tris buffer solutions containing 0-5 mM NaCl, leading to the colorimetric target recognition. The sharp melting transitions of the target-probe hybrids allow discrimination of single-base imperfection, including substitution, deletion, and insertion. The method works effectively in detecting sequences that are likely to form secondary structure. In addition, the study provides direct evidence of the relationship between the aggregate structure and the melting behavior of the DNA-linked nanoparticles.
    Keywords: Colorimetry -- Methods ; DNA -- Analysis ; Gold -- Chemistry ; Metal Nanoparticles -- Chemistry ; Sodium Chloride -- Chemistry
    ISSN: 00032700
    E-ISSN: 1520-6882
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  • 10
    In: Chemical Communications, 2013, Vol.49(43), pp.4959-4961
    Description: A real-time colorimetric assay utilizing gold nanoparticle networks and a duplex-specific nuclease (DSN) for label-free detection of microRNAs is proposed. Excellent sensitivity and selectivity were accomplished through the engagement of the DSN and a cumulative signal amplification process.
    Keywords: Gold -- Chemistry ; Metal Nanoparticles -- Chemistry ; Micrornas -- Analysis;
    ISSN: 1359-7345
    E-ISSN: 1364-548X
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