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  • 1
    In: EMBO Journal, 18 August 2010, Vol.29(16), pp.2675-2676
    Description: We are proud to announce our 2010 FOCUS ISSUE on the topic of Spatial Organization of Signalling. This editorial introduces you to the subjects that recognized experts discuss in their individual reviews. Sit back, read and enjoy….
    Keywords: Biology ; Chemistry;
    ISSN: 0261-4189
    E-ISSN: 1460-2075
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  • 2
    Language: English
    In: Nucleic acids research, August 2014, Vol.42(14), pp.8845-60
    Description: Phenotypically identical cells can dramatically vary with respect to behavior during their lifespan and this variation is reflected in their molecular composition such as the transcriptomic landscape. Single-cell transcriptomics using next-generation transcript sequencing (RNA-seq) is now emerging as a powerful tool to profile cell-to-cell variability on a genomic scale. Its application has already greatly impacted our conceptual understanding of diverse biological processes with broad implications for both basic and clinical research. Different single-cell RNA-seq protocols have been introduced and are reviewed here-each one with its own strengths and current limitations. We further provide an overview of the biological questions single-cell RNA-seq has been used to address, the major findings obtained from such studies, and current challenges and expected future developments in this booming field.
    Keywords: Gene Expression Profiling -- Methods ; High-Throughput Nucleotide Sequencing -- Methods ; Sequence Analysis, RNA -- Methods ; Single-Cell Analysis -- Methods
    ISSN: 03051048
    E-ISSN: 1362-4962
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  • 3
    Language: English
    In: Family Medicine & Primary Care Review, 01 September 2016, Vol.18(3), pp.387-390
    Description: The modern understanding of the quality of healthcare takes into account new roles that were granted to patients, including the possibility to assess healthcare based on their experience. A review of the literature shows that among the basic dimensions which serve to measure patients’ satisfaction, issues related to communication are essential. The aim of the study is to determine the aspects of communication with a family doctor which are particularly important from the perspective of the patient. A review of the literature from the years 2000–2016 on communication with family doctors was performed, including articles based on both quantitative and qualitative studies. The main source of the data was the English-language online database PubMed, in which articles were searched for based on such key words as: “medical consultation”, “family doctor”, “general practitioner”, “communication”, “patient’s perspective”. Furthermore, information was searched for in Polish-language journals, books and textbooks for physicians, using a method of manual screening. Analysis of the literature allowed for the identification of the following aspects of communicating with a family doctor perceived by patients: the experience of patients, establishing rapport with a patient, listening, informing a patient, non-verbal behavior, use of a computer during a visit, communication issues from older patients’ perspective, and the relationship between communication and dissatisfaction with care. In summary, the problems of communication between the patient and a family doctor are complex and diverse. The patients’ perspective, with regard to expectations and satisfaction with various aspects of care, including communication, is an important indicator of the quality of care delivered by a family physician.
    Keywords: Medical Consultation ; Family Doctor ; Communication ; Medicine
    ISSN: 1734-3402
    E-ISSN: 2449-8580
    Source: Directory of Open Access Journals (DOAJ)
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  • 4
    In: Nature, 2007, Vol.447(7145), p.730
    Description: DNA lesions interfere with DNA and RNA polymerase activity. Cyclobutane pyrimidine dimers and photoproducts generated by ultraviolet irradiation cause stalling of RNA polymerase II, activation of transcription-coupled repair enzymes, and inhibition of RNA synthesis. During the S phase of the cell cycle, collision of replication forks with damaged DNA blocks ongoing DNA replication while also triggering a biochemical signal that suppresses the firing of distant origins of replication. Whether the transcription machinery is affected by the presence of DNA double-strand breaks remains a long-standing question. Here we monitor RNA polymerase I (Pol I) activity in mouse cells exposed to genotoxic stress and show that induction of DNA breaks leads to a transient repression in Pol I transcription. Surprisingly, we find Pol I inhibition is not itself the direct result of DNA damage but is mediated by ATM kinase activity and the repair factor proteins NBS1 (also known as NLRP2) and MDC1. Using live-cell imaging, laser micro-irradiation, and photobleaching technology we demonstrate that DNA lesions interfere with Pol I initiation complex assembly and lead to a premature displacement of elongating holoenzymes from ribosomal DNA. Our data reveal a novel ATM/NBS1/MDC1-dependent pathway that shuts down ribosomal gene transcription in response to chromosome breaks. [PUBLICATION ]
    Keywords: Deoxyribonucleic Acid ; Ribonucleic Acids ; Breaking ; Polymerase ; Automated Teller Machines ; Replication ; Repair ; Inhibition ; General and Nonclassified (MD) ; General and Nonclassified (EC) ; General and Nonclassified (Ed) ; General and Nonclassified (Ep) ; Surveying, Theory, and Analysis (CE) ; Design Principles, Theory, and Analysis (Mt) ; Computing Milieux (General) (Ci) ; Electronics and Communications Milieux (General) (Ea) ; Solid State Milieux (General) (So) ; Article;
    ISSN: 0028-0836
    E-ISSN: 14764687
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  • 5
    Language: English
    In: Pediatria i Medycyna Rodzinna, 01 December 2015, Vol.11(4), pp.424-429
    Description: Erysipelas is  an acute, erythematous, rapidly spreading skin infection, usually caused by beta-haemolytic group A Streptococcus bacteria. The disease is usually located on the legs and toes, less frequently on the face. One of the predisposing factors for the development of erysipelas are coexisting infections. The aim of the work was to attempt to determine whether there was any connection between the atypical course of erysipelas and Helicobacter pylori infection in a 47-year-old female patient. The patient had a history of treatment for chronic otitis media with effusion and recurring abdominal pain. The current disease started abruptly with fever, erythematous skin lesion located on the right cheek and severe pain in the right ear. After a few days, the facial erythema got worse, oedema appeared on the right side of the face and redness, oedema and pain in the auricle could be observed. After examinations by an ENT specialist and a dermatologist, erysipelas of the face, auricle and external auditory meatus was diagnosed. The diagnosis was confirmed by bacteriological examination. As a result of antibiotic therapy skin lesions subsided and the patient’s general condition improved. As the dyspeptic symptoms exacerbated, a diagnostic test was performed and a coexisting Helicobacter pylori infection was diagnosed. The overall clinical picture and data obtained from medical literature suggest that the coexisting Helicobacter pylori infection could have contributed to both the chronic otitis media with effusion and atypical course of erysipelas. According to medical literature, in the case of patients with dyspeptic symptoms, Helicobacter pylori bacteria can be transferred from the lining of the stomach upwards to the oral cavity, middle ear and paranasal sinuses. Kariya et al. in their review of original work suggested that Helicobacter pylori may contribute to the exacerbation of an existing inflammation in the middle ear, which is a microaerophilic environment required for this bacteria to grow. In conclusion, it seems that constant stimulation of the immune system by Helicobacter pylori could have caused the system to become dysregulated and weakened, which probably accounted for the atypical course of erysipelas in the patient described.
    Keywords: Erysipelas ; Helicobacter Pylori ; Otitis Media With Effusion
    ISSN: 1734-1531
    E-ISSN: 2451-0742
    Source: Directory of Open Access Journals (DOAJ)
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  • 6
    In: Nature Reviews Microbiology, 2012, Vol.10(9), p.618
    Description: A comprehensive understanding of host-pathogen interactions requires a knowledge of the associated gene expression changes in both the pathogen and the host. Traditional, probe-dependent approaches using microarrays or reverse transcription PCR typically require the pathogen and host cells to be physically separated before gene expression analysis. However, the development of the probe-independent RNA sequencing (RNA-seq) approach has begun to revolutionize transcriptomics. Here, we assess the feasibility of taking transcriptomics one step further by performing 'dual RNA-seq', in which gene expression changes in both the pathogen and the host are analysed simultaneously.
    Keywords: Host-Pathogen Interactions ; Gene Expression Profiling -- Methods ; Sequence Analysis, RNA -- Methods;
    ISSN: 1740-1526
    E-ISSN: 17401534
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  • 7
    In: Problemy Pielęgniarstwa, 06/21/2016, Vol.1(1), pp.69-73
    Description: In recent years an increasing number of reports are being published which indicate that the occurrence of low back pain is a medical, social and professional issue among young people. Researchers express their concern about the fact that younger and younger generations complain about lower back pain, demonstrating the shift in the time of occurrence of this complaint. The aim of the study was to analyze the frequency of low back pain incidence among adolescents and young adults and to determine its cause and predisposing factors based on the review of medical literature. Polish and English-language study reports indicate that approximately 50% of people under 30 complain about low back pain. Additionally, over 60% of young people aged 14–17 admit to have had at least one incident of back pain in their life. One of the main reasons of low back pain among adolescents is a sedentary lifestyle. A number of studies emphasize how spinal diseases, including poor posture, affect the occurrence of lower back pain. Moreover, psychosocial factors are an important point of reference in the evaluation of non-specific lower back pain. Negative and depressive attitude as well as anxiety not only affect the occurrence of such condition at an early age, but also chronic lower back pain in adult life. Feeling of happiness, good sleep and good health are related to lack of complaints about lower back pain. The incidence of low back pain in adolescence is an important predictor of chronic lower back pain in adulthood. Therefore, it is necessary to take preventive measures related to lower back pain from the early school age. Nurses can play an important role in educating not only schoolchildren, but also their families.
    ISSN: 1233-9989
    E-ISSN: 2299-8284
    Source: CrossRef
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  • 8
    In: Nature Reviews Molecular Cell Biology, 2017
    Description: RNA is involved in the regulation of multiple cellular processes, often by forming sequence-specific base pairs with cellular RNA or DNA targets that must be identified among the large number of nucleic acids in a cell. Several RNA-based regulatory systems in eukaryotes, bacteria and archaea, including microRNAs (miRNAs), small interfering RNAs (siRNAs), CRISPR RNAs (crRNAs) and small RNAs (sRNAs) that are dependent on the RNA chaperone protein Hfq, achieve specificity using similar strategies. Central to their function is the presentation of short 'seed sequences' within a ribonucleoprotein complex to facilitate the search for and recognition of targets.
    Keywords: Micrornas -- Metabolism ; RNA, Small Interfering -- Metabolism;
    ISSN: 1471-0072
    E-ISSN: 1471-0080
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  • 9
    In: BioMed Research International, 2018, Vol.2018, 8 pages
    Description: . The sedentary lifestyle is defined as prolonged sitting both at work and during leisure time, with energy expenditures of below 600 MET · min/week. The sedentary lifestyle is a well-known predictor of obesity and other components of the metabolic syndrome. The influence of the sedentary lifestyle and associated factors on nsLBP is still being discussed.. The aim of this study was to assess the influence of a sedentary lifestyle and its associated metabolic predictors on the prevalence of nsLBP in nurses and paramedics.. The study included 609 participants, aged 30-60 years, who were residents of north-east Poland. Data was collected using a questionnaire (based, in part, on the Nordic Musculoskeletal Questionnaire), and included details of sociodemographic profile, chronic illnesses, and a short version of the International Physical Activity Questionnaire (IPAQ).. Nearly half (49.59%) of the respondents reported decreased physical activity, and in the group with recurring nsLBP this figure was 67.59%. Univariate logistic regression modelling found that leading a sedentary lifestyle caused a 3.5-fold increase in the incidence of recurring nsLBP (p〈0.001). Excessive coffee consumption significantly increased the likelihood of recurring LBP (OR=16.44, 95% CI: 8.55-31.61), and cigarette smoking increased the likelihood of both recurrent and chronic LBP. The likelihood of chronic low back pain was significantly increased by components of metabolic syndrome such as high blood pressure (over 9-fold), type 2 diabetes (over 3-fold), and hyperlipidemia (over 2-fold) (p〈0.001, p〈0.001, and p〈0.01, respectively).. A sedentary lifestyle significantly increased the incidence of recurring low back pain, while increased physical activity had a significant effect on the presence of chronic low back pain. In the sedentary lifestyle group, conditions classified within metabolic syndrome were found to significantly increase the chances of developing nonspecific low back pain.
    Keywords: Research Article
    ISSN: 2314-6133
    E-ISSN: 2314-6141
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  • 10
    Language: English
    In: Molecular Cell, 07 June 2018, Vol.70(5), pp.785-799
    Description: Bacteria are an exceedingly diverse group of organisms whose molecular exploration is experiencing a renaissance. While the classical view of bacterial gene expression was relatively simple, the emerging view is more complex, encompassing extensive post-transcriptional control involving riboswitches, RNA thermometers, and regulatory small RNAs (sRNAs) associated with the RNA-binding proteins CsrA, Hfq, and ProQ, as well as CRISPR/Cas systems that are programmed by RNAs. Moreover, increasing interest in members of the human microbiota and environmental microbial communities has highlighted the importance of understudied bacterial species with largely unknown transcriptome structures and RNA-based control mechanisms. Collectively, this creates a need for global RNA biology approaches that can rapidly and comprehensively analyze the RNA composition of a bacterium of interest. We review such approaches with a focus on RNA-seq as a versatile tool to investigate the different layers of gene expression in which RNA is made, processed, regulated, modified, translated, and turned over. RNA-seq-based approaches are revolutionizing how bacterial RNA biology can be studied. Hör, Gorski, and Vogel review the available global methods that can be used to chart the increasingly diverse number of RNA species and functions in any microbe of interest.
    Keywords: RNA-Seq ; Non-Coding RNA ; Small RNA ; Transcription ; RNA-Binding Protein ; Post-Transcriptional Control ; Biology
    ISSN: 1097-2765
    E-ISSN: 1097-4164
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