Kooperativer Bibliotheksverbund

Berlin Brandenburg

and
and

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Book
    Book
    Waidhofen a.d. Ybbs: Henneberg
    Description: 14, [1] p
    Description: Pr
    Keywords: French Language--Syntax
    Source: HathiTrust
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    Language: English
    In: Vaccine, 16 December 2013, Vol.31(52), pp.6194-6200
    Description: The non-structural protein NS1 of the influenza virus counteracts the interferon-mediated immune response of the host. We investigated the safety and immunogenicity of a trivalent formulation containing influenza H1N1, H3N2 and B strains lacking NS1 (delNS1-trivalent). Healthy adult study participants who were seronegative for at least one strain present in the vaccine formulation were randomized to receive a single intranasal dose of delNS1-trivalent vaccine at 7.0 log10 TCID50/subject ( = 39) or placebo ( = 41). Intranasal vaccination with the live replication-deficient delNS1-trivalent vaccine was well tolerated with no treatment-related serious adverse events. The most common adverse events identified, i.e. headache, oropharyngeal pain and rhinitis-like symptoms, were mainly mild and transient and distributed similarly in the treatment and placebo groups. Significant vaccine-specific immune responses were induced. Pre-existing low antibody titers or seronegativity for the corresponding vaccine strain yielded better response rates. We show that vaccination with a replication-deficient trivalent influenza vaccine containing H1N1, H3N2 and B strains lacking NS1 is safe and induces significant levels of antibodies (ClinicalTrials.gov identifier ).
    Keywords: Influenza ; Ns1 ; Intranasal ; Trivalent ; Live-Attenuated ; Reverse Genetics ; Medicine ; Biology ; Veterinary Medicine ; Pharmacy, Therapeutics, & Pharmacology
    ISSN: 0264-410X
    E-ISSN: 1873-2518
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 3
    Language: English
    In: The Journal of infectious diseases, 01 February 2010, Vol.201(3), pp.354-62
    Description: BACKGROUND. The nonstructural protein NS1 of influenza virus counteracts the interferon-mediated immune response of the host. By deleting the open reading frame of NS1, we have generated a novel type of influenza vaccine. We studied the safety and immunogenicity of an influenza strain lacking the NS1 gene (DeltaNS1-H1N1) in healthy volunteers. METHODS. Healthy seronegative adult volunteers were randomized to receive either a single intranasal dose of the DeltaNS1-H1N1 A/New Caledonia vaccine at 1 of 5 dose levels (6.4, 6.7, 7.0, 7.4, and 7.7 log(10) median tissue culture infective dose) (n = 36 recipients) or placebo (n = 12 recipients). RESULTS. Intranasal vaccination with the replication-deficient DeltaNS1-H1N1 vaccine was well tolerated. Rhinitis-like symptoms and headache were the most common adverse events identified during the 28-day observation period. Adverse events were similarly distributed between the treatment and placebo groups. Vaccine-specific local and serum antibodies were induced in a dose-dependent manner. In the highest dose group, vaccine-specific antibodies were detected in 10 of 12 volunteers. Importantly, the vaccine also induced neutralizing antibodies against heterologous drift variants. CONCLUSIONS. We show that vaccination with an influenza virus strain lacking the viral interferon antagonist NS1 induces statistically significant levels of strain-specific and cross-neutralizing antibodies despite the highly attenuated replication-deficient phenotype. Further studies are warranted to determine whether these results translate into protection from influenza virus infection. TRIAL REGISTRATION. ClinicalTrials.gov identifier: NCT00724997 .
    Keywords: Influenza A Virus, H1n1 Subtype -- Immunology ; Influenza Vaccines -- Immunology ; Influenza, Human -- Prevention & Control ; Vaccines, Attenuated -- Immunology ; Viral Nonstructural Proteins -- Genetics
    ISSN: 00221899
    E-ISSN: 1537-6613
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 4
    In: Analytical Chemistry, Dec 1, 1996, Vol.68(23), p.4300(4)
    Description: Capillary isoelectric focusing was applied to determine the pI value of human rhinovirus serotype 2 (HRV 2), a picornavirus of about 8 500 000 Da in size. Using fused silica capillaries dynamically coated with hydroxypropylmethyl cellulose (added at 0.08% to the catholyte), the virus zone failed to reach the steady state position in the pH gradient within times usually employed in focusing experiments, as the electroosmotic flow (EOF) pushed the analyte zone past the detector. Therefore, the residence time of the zones in the separation capillary was extended by applying hydrodynamic pressure at the detector side during focusing, thus pneumatically counteracting the EOF. After completion of focusing, the zones were mobilized by pressure maintaining the high voltage. For calibration of the pH gradient, low molecular mass pI marker substances were employed. Using the relation between the apparent pI value of the virus and the focusing time under counter pressure, the actual pI of HRV2 was determined as 6.8 by extrapolating to infinite time.
    Keywords: Rhinoviruses -- Analysis ; Isoelectric Focusing -- Usage
    ISSN: 0003-2700
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 5
    Language: English
    In: Analytical Chemistry, 01/1996, Vol.68(23), pp.4300-4303
    ISSN: 0003-2700
    E-ISSN: 1520-6882
    Source: American Chemical Society (via CrossRef)
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 6
    Language: English
    In: Journal of Chromatography A, 1996, Vol.738(1), pp.123-128
    Description: A theory of peak dispersion in capillary isoelectric focusing arising when mobilisation is carried out by pressure is presented and proved experimentally. Contributions of flow profile and wall adsorption to zone broadening are evaluated, varying the viscosity and surface activity by addition of a polymer (derivatized cellulose). The chromatographic model of the contribution of adsorption to the plate height is applied. The plate height is determined for a protein (myoglobin) as a function of pressure, voltage and concentration of polymer additive. According to the theoretical model, due to adsorption, increasing peak broadening is found with increasing velocity of mobilisation and decreasing polymer concentration. The predicted influence of the direction of pH gradient on peak dispersion during mobilisation is demonstrated.
    Keywords: Dispersion ; Adsorption ; Coating ; Zone Mobilisation ; Proteins ; Chemistry
    ISSN: 0021-9673
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 7
    Language: English
    In: Vaccine
    Description: Traditional inactivated influenza vaccines are the type of vaccines that were most frequently developed for immunization against the highly pathogenic avian H5N1 influenza virus. However, clinical trials with inactivated influenza vaccines for H5N1 indicated that high doses and at least two immunizations are required for an effective immune response (Nicholson et al., 2001; Treanor, Campbell et al., 2006; Treanor, Schiff et al., 2006; Ehrlich et al., 2008). We investigated the safety and immunogenicity of a live attenuated H5N1 vaccine (delNS1-H5N1) lacking the interferon antagonist nonstructural protein 1 (NS1). We conducted a double-blind, placebo-controlled, phase 1 study in healthy adult participants who were randomly assigned at a 2:1 ratio to receive two immunizations of delNS1-H5N1 vaccine at 6.8 log10 50% tissue culture infectious doses (TCID )/subject or 7.5 log10 TCID /subject, or placebo. Intranasal vaccination with the live attenuated delNS1-H5N1 vaccine was safe and well tolerated. The most common adverse events identified were symptoms associated with mild influenza infections, such as increased body temperature (〉37.0 °C), pharyngeal erythema, rhinitis and throat irritation, and were reported within 7 days after the first immunization. delNS1-H5N1 was able to induce significant vaccine-specific serum antibody titers even at the lower dose level of 6.8 log10 TCID /subject. Seroconversion occurred in 75% of study participants after only one immunization with 7.5 log10 TCID /subject. Vaccine-specific local IgA responses were observed in 41.7% of individuals that showed serum antibody responses after 2nd immunization. We show that vaccination with a live attenuated H5N1 influenza vaccine lacking NS1 is safe and induces significant levels of vaccine-specific antibodies even after one immunization. The safety and immunogenicity data indicate that delNS1-H5N1 has the potential to fulfil the unmet need for an effective influenza vaccine in pandemic situations. ( identifier NCT03745274).
    Keywords: Influenza ; Ns1 ; Intranasal ; H5n1 ; Live Attenuated ; Replication-Deficient ; Reverse Genetics ; Medicine ; Biology ; Veterinary Medicine ; Pharmacy, Therapeutics, & Pharmacology
    ISSN: 0264-410X
    E-ISSN: 1873-2518
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 8
    Language: English
    In: Journal of Neuroscience Methods, 2000, Vol.95(1), pp.47-53
    Description: We present a computer-guided microelectrode positioning system that is routinely used in our laboratory for intracellular electrophysiology and functional staining of retinal neurons. Wholemount preparations of isolated retina are kept in a superfusion chamber on the stage of an inverted microscope. Cells and layers of the retina are visualized by Nomarski interference contrast using infrared light in combination with a CCD camera system. After five-point calibration has been performed the electrode can be guided to any point inside the calibrated volume without moving the retina. Electrode deviations from target cells can be corrected by the software further improving the precision of this system. The good visibility of cells avoids prelabeling with fluorescent dyes and makes it possible to work under completely dark adapted conditions.
    Keywords: Retina ; Intracellular ; Inverted Microscope ; Infrared Interference Contrast ; Computer-Guided Cell Impalement ; Electrophysiology ; Medicine ; Anatomy & Physiology
    ISSN: 0165-0270
    E-ISSN: 1872-678X
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 9
Close ⊗
This website uses cookies and the analysis tool Matomo. Further information can be found on the KOBV privacy pages