Environmental pollution, 2010, Vol.158, pp.977-982
Tropospheric ozone causes severe oxidative stress in plants. To investigate the transcriptional responsiveness of adult trees to ozone, fully-expanded sun and shade leaves of mature beech trees were harvested at four time points over the entire vegetation period in 2005 and 2006. Microarray analyses were conducted on leaves from trees grown in the field under ambient and twice-ambient ozone concentrations at Kranzberger Forst (Bavaria). Beech trees changed their transcript levels in response to ozone. In the years 2005 and 2006 different transcription patterns were observed; this may have been a result of different weather conditions and ozone uptake. Furthermore, we obtained differences in mRNA expression patterns between shade and sun leaves. In the ozone-treated sun leaves of 2005, slightly up- and down-regulated transcript levels were detected, particularly in the spring and autumn, whereas shade leaves clearly exhibited reduced mRNA levels, particularly at the end of the vegetation period. In 2006, this pattern could not be confirmed, and in the autumn, four other transcripts were slightly up-regulated in ozone-treated shade leaves. In addition, two other transcripts were found to be influenced in sun leaves in the spring/summer. While we detected changes in the levels of only a few transcripts, the observed effects were not identical in both years. In conclusion, elevated ozone exhibited very small influence on the transcription levels of genes of mature beech trees. At the transcriptional level, leaves of mature beech trees barely react to double ambient ozone concentrations; differences are detected primarily between sun/shade leaves and between different growing seasons. ; In the special issue: Facing the Future: Evidence from Joint AspenFACE, SoyFACE and SFB 607 Meeting / edited by Kevin Percy, John King and Rainer Matyssek. Paper presented at meeting held April 2-4, 2008, Rhinelander, Wisconsin. Includes references ; p. 977-982.
Ozone ; Microarray Technology ; Forest Trees ; Fagus Sylvatica ; Elevated Atmospheric Gases ; Leaves ; Transcription (Genetics) ; Reverse Transcriptase Polymerase Chain Reaction ; Air Pollution
AGRIS (Food and Agriculture Organization of the United Nations)
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