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  • 1
    Language: English
    In: The Journal of biological chemistry, 09 November 2018, Vol.293(45), pp.17559-17573
    Description: The supramolecular organization of membrane proteins (MPs) is sensitive to environmental changes in photosynthetic organisms. Isolation of MP supercomplexes from the green algae , which are believed to contribute to cyclic electron flow (CEF) between the cytochrome complex (Cyt- ) and photosystem...
    Keywords: Chlamydomonas ; Cytochrome B6f Complex ; Electron Transfer Complex ; Membrane Protein ; Photosynthesis ; Protein Cross-Linking ; Chlamydomonas Reinhardtii -- Enzymology ; Cytochrome B6f Complex -- Metabolism ; Photosystem I Protein Complex -- Metabolism
    E-ISSN: 1083-351X
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  • 2
    Language: English
    In: PLoS ONE, 2012, Vol.7(7), p.e40717
    Description: The IGROVCDDP cisplatin-resistant ovarian cancer cell line is also resistant to paclitaxel and models the resistance phenotype of relapsed ovarian cancer patients after first-line platinum/taxane chemotherapy. A TaqMan low-density array (TLDA) was used to characterise the expression of 380 genes associated with chemotherapy resistance in IGROVCDDP cells. Paclitaxel resistance in IGROVCDDP is mediated by gene and protein overexpression of P-glycoprotein and the protein is functionally active. Cisplatin resistance was not reversed by elacridar, confirming that cisplatin is not a P-glycoprotein substrate. Cisplatin resistance in IGROVCDDP is multifactorial and is mediated in part by the glutathione pathway and decreased accumulation of drug. Total cellular glutathione was not increased. However, the enzyme activity of GSR and GGT1 were up-regulated. The cellular localisation of copper transporter CTR1 changed from membrane associated in IGROV-1 to cytoplasmic in IGROVCDDP. This may mediate the previously reported accumulation defect. There was decreased expression of the sodium potassium pump (ATP1A), MRP1 and FBP which all have been previously associated with platinum accumulation defects in platinum-resistant cell lines. Cellular localisation of MRP1 was also altered in IGROVCDDP shifting basolaterally, compared to IGROV-1. BRCA1 was also up-regulated at the gene and protein level. The overexpression of P-glycoprotein in a resistant model developed with cisplatin is unusual. This demonstrates that P-glycoprotein can be up-regulated as a generalised stress response rather than as a specific response to a substrate. Mechanisms characterised in IGROVCDDP cells may be applicable to relapsed ovarian cancer patients treated with frontline platinum/taxane chemotherapy.
    Keywords: Research Article ; Biology ; Chemistry ; Medicine ; Chemistry ; Molecular Biology ; Oncology ; Biochemistry
    E-ISSN: 1932-6203
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  • 3
    Language: English
    In: Molecular Plant, 07 August 2017, Vol.10(8), pp.1107-1125
    Description: Thiol-based redox post-translational modifications have emerged as important mechanisms of signaling and regulation in all organisms, and thioredoxin plays a key role by controlling the thiol-disulfide status of target proteins. Recent redox proteomic studies revealed hundreds of proteins regulated by glutathionylation and nitrosylation in the unicellular green alga , while much less is known about the thioredoxin interactome in this organism. By combining qualitative and quantitative proteomic analyses, we have comprehensively investigated the thioredoxome and 1188 targets have been identified. They participate in a wide range of metabolic pathways and cellular processes. This study broadens not only the redox regulation to new enzymes involved in well-known thioredoxin-regulated metabolic pathways but also sheds light on cellular processes for which data supporting redox regulation are scarce (aromatic amino acid biosynthesis, nuclear transport, etc). Moreover, we characterized 1052 thioredoxin-dependent regulatory sites and showed that these data constitute a valuable resource for future functional studies in . By comparing this thioredoxome with proteomic data for glutathionylation and nitrosylation at the protein and cysteine levels, this work confirms the existence of a complex redox regulation network in and provides evidence of a tremendous selectivity of redox post-translational modifications for specific cysteine residues. Thioredoxin plays a key role by controlling the thiol-disulfide status of their target proteins. We identified 1188 thioredoxin targets and 1052 regulatory sites in the unicellular green alga . By comparing this thioredoxome with other proteomic data for glutathionylation and nitrosylation at the protein and cysteine levels, this work confirms the existence of a complex redox regulation network in and provides evidence of a tremendous selectivity of redox post-translational modifications for specific cysteine residues.
    Keywords: Chlamydomonas Reinhardtii ; Disulfide Bond ; Isotope-Coded Affinity Tag ; Redox Proteomics ; Redox Regulation ; Thioredoxin Targets ; Botany
    ISSN: 1674-2052
    E-ISSN: 1752-9867
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  • 4
    Language: English
    In: International Journal of Evolutionary Biology, Annual, 2011
    Description: Miniature Inverted-repeat Transposable Elements (MITEs) are small nonautonomous class-II transposable elements distributed throughout eukaryotic genomes. We identified a novel family of MITEs (named Alex)inthe Coffea canephora genome often associated with expressed sequences. The Alex-1 element is inserted in an intron of a gene at the CcEIN4 locus. Its mobility was demonstrated by sequencing the insertion site in C. canephora accessions and Coffea species. Analysis of the insertion polymorphism of Alex-1 at this locus in Coffea species and in C. canephora showed that there was no relationship between the geographical distribution of the species, their phylogenetic relationships, and insertion polymorphism. The intraspecific distribution of C. canephora revealed an original situation within the E diversity group. These results suggest possibly greater gene flow between species than previously thought. This MITE family will enable the study of the C. canephora genome evolution, phylogenetic relationships, and possible gene flows within the Coffea genus.
    Keywords: Genetic Polymorphisms -- Identification And Classification ; Coffee (Plant) -- Genetic Aspects ; Gene Flow -- Research ; Transposons -- Identification And Classification
    ISSN: 2090-052X
    Source: Cengage Learning, Inc.
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  • 5
    Language: English
    In: International Journal of Evolutionary Biology, Annual, 2011
    Description: Miniature Inverted-repeat Transposable Elements (MITEs) are small nonautonomous class-II transposable elements distributed throughout eukaryotic genomes. We identified a novel family of MITEs (named Alex)inthe Coffea canephora genome often associated with expressed sequences. The Alex-1 element is inserted in an intron of a gene at the CcEIN4 locus. Its mobility was demonstrated by sequencing the insertion site in C. canephora accessions and Coffea species. Analysis of the insertion polymorphism of Alex-1 at this locus in Coffea species and in C. canephora showed that there was no relationship between the geographical distribution of the species, their phylogenetic relationships, and insertion polymorphism. The intraspecific distribution of C. canephora revealed an original situation within the E diversity group. These results suggest possibly greater gene flow between species than previously thought. This MITE family will enable the study of the C. canephora genome evolution, phylogenetic relationships, and possible gene flows within the Coffea genus.
    Keywords: Genetic Polymorphisms -- Identification And Classification ; Coffee (Plant) -- Genetic Aspects ; Gene Flow -- Research ; Transposons -- Identification And Classification
    ISSN: 2090-052X
    Source: Cengage Learning, Inc.
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  • 6
    Language: English
    In: BMC Plant Biology, 2009, Vol.9, p.22
    Description: BACKGROUND: Coffea canephora, also called Robusta, belongs to the Rubiaceae, the fourth largest angiosperm family. This diploid species (2x = 2n = 22) has a fairly small genome size of approximately 690 Mb and despite its extreme economic importance, particularly for developing countries,...
    Keywords: Chromosomes, Artificial, Bacterial ; Coffea ; Plant Proteins ; Receptors, Cell Surface ; Sequence Alignment ; Sequence Analysis, DNA ; Vitis ; Conserved Sequence ; DNA, Plant ; Evolution, Molecular ; Gene Library ; Genes, Plant ; Genome, Plant ; Molecular Sequence Data ; Physical Chromosome Mapping ; Life Sciences ; Biochemistry, Molecular Biology ; Life Sciences ; Cellular Biology ; Life Sciences
    ISSN: 1471-2229
    E-ISSN: 1471-2229
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  • 7
    Language: English
    Description: The rationale behind the research presented in this thesis grew from a perception that, historically, work that was seen to be
    Keywords: Dressmaker ; Dressmaking ; Fashion ; New Zealand
    Source: Networked Digital Library of Theses and Dissertations
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  • 8
    Language: English
    In: BMC Plant Biology, Feb 25, 2009, Vol.9, p.22
    Description: Background Coffea canephora, also called Robusta, belongs to the Rubiaceae, the fourth largest angiosperm family. This diploid species (2x = 2n = 22) has a fairly small genome size of [almost equal to] 690 Mb and despite its extreme economic importance, particularly for developing countries, knowledge on the genome composition, structure and evolution remain very limited. Here, we report the 160 kb of the first C. canephora Bacterial Artificial Chromosome (BAC) clone ever sequenced and its fine analysis. Results This clone contains the CcEIN4 gene, encoding an ethylene receptor, and twenty other predicted genes showing a high gene density of one gene per 7.8 kb. Most of them display perfect matches with C. canephora expressed sequence tags or show transcriptional activities through PCR amplifications on cDNA libraries. Twenty-three transposable elements, mainly Class II transposon derivatives, were identified at this locus. Most of these Class II elements are Miniature Inverted-repeat Transposable Elements (MITE) known to be closely associated with plant genes. This BAC composition gives a pattern similar to those found in gene rich regions of Solanum lycopersicum and Medicago truncatula genomes indicating that the CcEIN4 regions may belong to a gene rich region in the C. canephora genome. Comparative sequence analysis indicated an extensive conservation between C. canephora and most of the reference dicotyledonous genomes studied in this work, such as tomato (S. lycopersicum), grapevine (V. vinifera), barrel medic M. truncatula, black cottonwood (Populus trichocarpa) and Arabidopsis thaliana. The higher degree of microcollinearity was found between C. canephora and V. vinifera, which belong respectively to the Asterids and Rosids, two clades that diverged more than 114 million years ago. Conclusion This study provides a first glimpse of C. canephora genome composition and evolution. Our data revealed a remarkable conservation of the microcollinearity between C. canephora and V. vinifera and a high conservation with other distant dicotyledonous reference genomes. Altogether, these results provide valuable information to identify candidate genes in C. canephora genome and serve as a foundation to establish strategies for whole genome sequencing. Future large-scale sequence comparison between C. canephora and reference sequenced genomes will help in understanding the evolutionary history of dicotyledonous plants.
    Keywords: Coffee (Plant) -- Genetic Aspects ; Coffee (Plant) -- Natural History ; Coffee (Plant) -- Research ; Ethylene -- Genetic Aspects ; Ethylene -- Research ; Grapes -- Genetic Aspects ; Grapes -- Research
    ISSN: 1471-2229
    Source: Cengage Learning, Inc.
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  • 9
    Language: English
    In: BMC Plant Biology, Feb 25, 2009, Vol.9, p.22
    Description: Background Coffea canephora, also called Robusta, belongs to the Rubiaceae, the fourth largest angiosperm family. This diploid species (2x = 2n = 22) has a fairly small genome size of [almost equal to] 690 Mb and despite its extreme economic importance, particularly for developing countries, knowledge on the genome composition, structure and evolution remain very limited. Here, we report the 160 kb of the first C. canephora Bacterial Artificial Chromosome (BAC) clone ever sequenced and its fine analysis. Results This clone contains the CcEIN4 gene, encoding an ethylene receptor, and twenty other predicted genes showing a high gene density of one gene per 7.8 kb. Most of them display perfect matches with C. canephora expressed sequence tags or show transcriptional activities through PCR amplifications on cDNA libraries. Twenty-three transposable elements, mainly Class II transposon derivatives, were identified at this locus. Most of these Class II elements are Miniature Inverted-repeat Transposable Elements (MITE) known to be closely associated with plant genes. This BAC composition gives a pattern similar to those found in gene rich regions of Solanum lycopersicum and Medicago truncatula genomes indicating that the CcEIN4 regions may belong to a gene rich region in the C. canephora genome. Comparative sequence analysis indicated an extensive conservation between C. canephora and most of the reference dicotyledonous genomes studied in this work, such as tomato (S. lycopersicum), grapevine (V. vinifera), barrel medic M. truncatula, black cottonwood (Populus trichocarpa) and Arabidopsis thaliana. The higher degree of microcollinearity was found between C. canephora and V. vinifera, which belong respectively to the Asterids and Rosids, two clades that diverged more than 114 million years ago. Conclusion This study provides a first glimpse of C. canephora genome composition and evolution. Our data revealed a remarkable conservation of the microcollinearity between C. canephora and V. vinifera and a high conservation with other distant dicotyledonous reference genomes. Altogether, these results provide valuable information to identify candidate genes in C. canephora genome and serve as a foundation to establish strategies for whole genome sequencing. Future large-scale sequence comparison between C. canephora and reference sequenced genomes will help in understanding the evolutionary history of dicotyledonous plants.
    Keywords: Coffee (Plant) -- Genetic Aspects ; Coffee (Plant) -- Natural History ; Coffee (Plant) -- Research ; Ethylene -- Genetic Aspects ; Ethylene -- Research ; Grapes -- Genetic Aspects ; Grapes -- Research
    ISSN: 1471-2229
    Source: Cengage Learning, Inc.
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  • 10
    In: European Journal of Organic Chemistry, 01 September 2017, Vol.2017(32), pp.4803-4819
    Description: Mannosylglycerate (MG) is a compatible solute extracted from some red algae and bacteria. Thanks to its ability to protect proteins and stabilise enzymes, MG has been widely studied for its uses against neurodegenerative diseases, and also in biotechnology. More recently, its immunostimulating properties against chronic lymphocytic leukaemia have been investigated. In this paper, we report the synthesis of MG derivatives, and a preliminary biological study on their capacity to behave as immunostimulating agents. We investigated their toxicity against peripheral blood mononuclear cells from healthy donors and their ability to increase the phagocytosis of opsonised bacteria by polynuclear neutrophils. This resulted in the discovery of two new molecules as potential immunostimulating agents. Analogues of mannosylglycerate have been synthesised and evaluated as immunostimulating agents.
    Keywords: Carbohydrates ; Immunology ; Biological Activity ; Glycosides ; Structure‐Activity Relationships
    ISSN: 1434-193X
    E-ISSN: 1099-0690
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